################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspLU11I BsrDI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraII DrdI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflIII 122 ACGTGT A^C[GA][CT]GT AlwI 282 GATCC GGATC(4/5) Aor51HI 257 AGCGCT AGC^GCT Asp700I 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 144 GGTCC G^G[AT]CC BalI 320 TGGCCA TGG^CCA BanI 343 GGCACC G^G[CT][GA]CC BanII 39 GGGCTC G[GA]GC[CT]^C BcnI 310 CCCGG CC^[GC]GG BsaAI 121 TACGTG [CT]AC^GT[GA] BsiEI 290 CGGCCG CG[GA][CT]^CG Bsp1407I 125 TGTACA T^GTACA BspHI 185 TCATGA T^CATGA BspMI 141 GCAGGT ACCTGC(4/8) BsrBI 20 CCGCTC CCGCTC(-3/-3) BsrGI 125 TGTACA T^GTACA BstEII 354 GGTCACC G^GT[ACGT]ACC BstF5I 272 CATCC GGATG(2/0) BstNI 240 CCTGG CC^[AT]GG BstPI 354 GGTCACC G^GT[ACGT]ACC BstXI 170 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 281 AGATCC [GA]^GATC[CT] DpnII 282 GATC ^GATC DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 176 CCATGG C^C[GA][CT]GG EagI 290 CGGCCG C^GGCCG EclXI 290 CGGCCG C^GGCCG Eco47III 257 AGCGCT AGC^GCT Eco52I 290 CGGCCG C^GGCCG EcoO65I 354 GGTCACC G^GT[ACGT]ACC EcoRII 240 CCTGG ^CC[AT]GG FokI 272 CATCC GGATG(9/13) HaeII 257 AGCGCT [GA]GCGC^[CT] HincII 199 GTCAAC GT[CT]^[GA]AC HindII 199 GTCAAC GT[CT]^[GA]AC MaeII 122 ACGT A^CGT MboI 282 GATC ^GATC MflI 281 AGATCC [GA]^GATC[CT] MluNI 320 TGGCCA TGG^CCA MscI 320 TGGCCA TGG^CCA MslI 117 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 240 CCTGG CC^[AT]GG NciI 310 CCCGG CC^[GC]GG NcoI 176 CCATGG C^CATGG NdeII 282 GATC ^GATC RcaI 185 TCATGA T^CATGA Sau3AI 282 GATC ^GATC SspBI 125 TGTACA T^GTACA TaqI 212 TCGA T^CGA TthHB8I 212 TCGA T^CGA XhoII 281 AGATCC [GA]^GATC[CT] XmnI 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 222 CGCG CG^CG 277 CGCG AfaI 126 GTAC GT^AC 363 GTAC BmyI 39 GGGCTC G[AGT]GC[ACT]^C 342 GGGCAC Bsp1286I 39 GGGCTC G[AGT]GC[ACT]^C 342 GGGCAC BsrFI 9 ACCGGC [GA]^CCGG[CT] 157 ACCGGC BssKI 240 CCTGG ^CC[ACGT]GG 310 CCCGG BstUI 222 CGCG CG^CG 277 CGCG Cfr10I 9 ACCGGC [GA]^CCGG[CT] 157 ACCGGC Cfr13I 27 GGCCC G^G[ACGT]CC 144 GGTCC EaeI 290 CGGCCG [CT]^GGCC[GA] 320 TGGCCA HphI 56 TCACC GGTGA(8/7) 356 TCACC MaeIII 55 GTCAC ^GT[ACGT]AC 355 GTCAC MboII 69 GAAGA GAAGA(8/7) 209 TCTTC MnlI 229 GAGG CCTC(7/6) 340 GAGG MvnI 222 CGCG CG^CG 277 CGCG NlaIII 177 CATG CATG^ 186 CATG RsaI 126 GTAC GT^AC 363 GTAC Sau96I 27 GGCCC G^G[ACGT]CC 144 GGTCC ScrFI 240 CCTGG CC^[ACGT]GG 310 CCCGG Tsp45I 55 GTCAC ^GT[GC]AC 355 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI Cac8I CfoI EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MspI MwoI NlaIV SfaNI StyI TseI