Restriction map for pFN21AE1615
 (Circular) (Six-base) MAPSORT of: pFN21AE1615.seq  Check: 7835  from: 1  to: 5083



pFN21AE1615  5083 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:48  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2893     278 
   Size:         53      83     186     936    1357    2468

  Fragments arranged by size: 

                2468    1357     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2426    1937 
   Size:        489    4594
 
Acc65I G'GTAC_C

Cuts at:   2404    2404 
   Size:       5083
 
AclI AA'CG_TT

Cuts at:   1563    3210    3583    1563 
   Size:       1647     373    3063

  Fragments arranged by size: 

                3063    1647     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2162    3146    4530    4798    1632 
   Size:        530     984    1384     268    1917

  Fragments arranged by size: 

                1917    1384     984     530     268
 
AfeI AGC'GCT

Cuts at:   2264    2264 
   Size:       5083
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4886
 
AflIII A'CryG_T

Cuts at:   1203    2127    3982    4959    1203 
   Size:        924    1855     977    1327

  Fragments arranged by size: 

                1855    1327     977     924
 
AgeI A'CCGG_T

Cuts at:   3914    3914 
   Size:       5083
 
AhdI GACnn_n'nnGTC

Cuts at:   3812    3812 
   Size:       5083
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5083
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5083
 
AlwNI CAG_nnn'CTG

Cuts at:   2826    4398    2826 
   Size:       1572    3511
 
ApaLI G'TGCA_C

Cuts at:   3140    4296    3140 
   Size:       1156    3927
 
ApoI r'AATT_y

Cuts at:   1493    2392    2618    2770    4879    1493 
   Size:        899     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     899     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3637     160 
   Size:        872    2605    1606

  Fragments arranged by size: 

                2605    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5083
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2408    1870 
   Size:         73     465    4545

  Fragments arranged by size: 

                4545     465      73
 
BamHI G'GATC_C

Cuts at:   2413    2780    2413 
   Size:        367    4716
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2348    2404    3859     618 
   Size:        325     217     627     561      56    1455    1842

  Fragments arranged by size: 

                1842    1455     627     561     325     217      56
 
BanII G_rGCy'C

Cuts at:   1391    2048    2402    1391 
   Size:        657     354    4072

  Fragments arranged by size: 

                4072     657     354
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4112
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3274    3308    1903 
   Size:         34      50      34    1253      34    3678

  Fragments arranged by size: 

                3678    1253      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2975    4185    2975 
   Size:       1210    3873
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5083
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3694     136 
   Size:        107     122      71     631     605    2022    1525

  Fragments arranged by size: 

                2022    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5078    5078 
   Size:       5083
 
BlpI GC'TnA_GC

Cuts at:   2500    2500 
   Size:       5083
 
Bme1580I G_kGCm'C

Cuts at:   1790    2351    3144    4300    1790 
   Size:        561     793    1156    2573

  Fragments arranged by size: 

                2573    1156     793     561
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5083
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2814    3772     442 
   Size:        416      53      83     399      24    1397     958    1753

  Fragments arranged by size: 

                1753    1397     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5083
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2134    2134 
   Size:       5083
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3743    1239 
   Size:       2504    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3208    4073    4371    3208 
   Size:        865     298    3920

  Fragments arranged by size: 

                3920     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3746     882 
   Size:        617    2247    2219

  Fragments arranged by size: 

                2247    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2128    2874     493 
   Size:        711     924     746    2702

  Fragments arranged by size: 

                2702     924     746     711
 
BsaBI GATnn'nnATC

Cuts at:   5077    5077 
   Size:       5083
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2890    3272    3907     275 
   Size:         53      83     186     936    1357     382     635    1451

  Fragments arranged by size: 

                1451    1357     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3516    3914    4188    4335    4746    3516 
   Size:        398     274     147     411    3853

  Fragments arranged by size: 

                3853     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2249    2279    2360    2390    1178 
   Size:         30     165      30     846      30      81      30    3871

  Fragments arranged by size: 

                3871     846     165      81      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5083
 
BseYI C'CCAG_C

Cuts at:   4286    4782    4286 
   Size:        496    4587
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4510
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2298    2533    3294    4322    1540 
   Size:        275     186     297     235     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     297     275     235     186
 
BsiHKAI G_wGCw'C

Cuts at:   3144    3229    4300    3144 
   Size:         85    1071    3927

  Fragments arranged by size: 

                3927    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2594    2687    2594 
   Size:         93    4990
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2048    2351    2402    3144    3229    4300    1391 
   Size:        399     258     303      51     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     303     258      85      51
            
 
BspEI T'CCGG_A

Cuts at:   4746    4746 
   Size:       5083
 
BspHI T'CATG_A

Cuts at:   2190    2972    4702    2190 
   Size:        782    1730    2571

  Fragments arranged by size: 

                2571    1730     782
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2137    2438     844 
   Size:       1293     301    3489

  Fragments arranged by size: 

                3489    1293     301
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2027    2970    1394 
   Size:        429     204     943    3507

  Fragments arranged by size: 

                3507     943     429     204
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3578    3752      65 
   Size:       1183    2330     174    1396

  Fragments arranged by size: 

                2330    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2014    2162    3727    3914    1700 
   Size:        110     204     148    1565     187    2869

  Fragments arranged by size: 

                2869    1565     204     187     148     110
 
BsrGI T'GTAC_A

Cuts at:     96    2130      96 
   Size:       2034    3049
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5083
 
BssSI C'ACGA_G

Cuts at:   3143    4155    3143 
   Size:       1012    4071
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5083
 
BstBI TT'CG_AA

Cuts at:   3887    3887 
   Size:       5083
 
BstEII G'GTnAC_C

Cuts at:   1184    2359    1184 
   Size:       1175    3908
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2182    2182 
   Size:       5083
 
BstYI r'GATC_y

Cuts at:   1972    2286    2413    2780    3165    3182    4634    4720    4732 
   Size:        314     127     367     385      17    1452      86      12

Cuts at:   4732    5078    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     314     127      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2181     513 
   Size:        552    1116    3415

  Fragments arranged by size: 

                3415    1116     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2209     487 
   Size:        683      56     983    3361

  Fragments arranged by size: 

                3361     983     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2599    3393    3413    2599 
   Size:        794      20    4269

  Fragments arranged by size: 

                4269     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5083
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5048
 
DraI TTT'AAA

Cuts at:   2387    2387 
   Size:       5083
 
DraIII CAC_nnn'GTG

Cuts at:   2338    2338 
   Size:       5083
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4090     809 
   Size:        507    2774    1802

  Fragments arranged by size: 

                2774    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2295    2325    2530 
   Size:         54    1425      50     275      18     465      30     205

Cuts at:   2530    3419    5048       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     465     275     205      54      50
                  43      30      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2295    2530    1537 
   Size:        275     483     235    4090

  Fragments arranged by size: 

                4090     483     275     235
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3013    1353 
   Size:       1660    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3670    4044    4190     387 
   Size:       1064     138    2081     374     146    1280

  Fragments arranged by size: 

                2081    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2162    3146    3743    4530    4798    1239 
   Size:        393     530     984     597     787     268    1524

  Fragments arranged by size: 

                1524     984     787     597     530     393     268
 
EcoRI G'AATT_C

Cuts at:   2392    2770    2392 
   Size:        378    4705
 
FspI TGC'GCA

Cuts at:   3589    3589 
   Size:       5083
 
HaeII r_GCGC'y

Cuts at:   1678    2266    4230    1678 
   Size:        588    1964    2531

  Fragments arranged by size: 

                2531    1964     588
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3724    3756    3798    3830    1373 
   Size:         32     135      32    2152      32      42      32    2626

  Fragments arranged by size: 

                2626    2152     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2206    2427    2673     669 
   Size:        988     281     268     221     246    3079

  Fragments arranged by size: 

                3079     988     281     268     246     221
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5083
 
HpaI GTT'AAC

Cuts at:   2673    2673 
   Size:       5083
 
KpnI G_GTAC'C

Cuts at:   2408    2408 
   Size:       5083
 
MfeI C'AATT_G

Cuts at:   2682    2682 
   Size:       5083
 
MluI A'CGCG_T

Cuts at:   4959    4959 
   Size:       5083
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4197    4381    1305 
   Size:       2892     184    2007

  Fragments arranged by size: 

                2892    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2327      10 
   Size:         54    1425     343     495    2766

  Fragments arranged by size: 

                2766    1425     495     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2126    3041    3400    3559     518 
   Size:        648     960     915     359     159    2042

  Fragments arranged by size: 

                2042     960     915     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2499    3176    4324    4569    1606 
   Size:        893     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     893     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2181     513 
   Size:        552    1116    3415

  Fragments arranged by size: 

                3415    1116     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5083
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5083
 
NotI GC'GGCC_GC

Cuts at:   2530    2530 
   Size:       5083
 
NspI r_CATG'y

Cuts at:   1132    1229    2441    3986    1132 
   Size:         97    1212    1545    2229

  Fragments arranged by size: 

                2229    1545    1212      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5083
 
PciI A'CATG_T

Cuts at:   3982    3982 
   Size:       5083
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5083
 
PmeI GTTT'AAAC

Cuts at:   2387    2387 
   Size:       5083
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3152    3184    4665    4697    3152 
   Size:         32    1481      32    3538

  Fragments arranged by size: 

                3538    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5083
 
PsiI TTA'TAA

Cuts at:   2653    2653 
   Size:       5083
 
PstI C_TGCA'G

Cuts at:    830    2435     830 
   Size:       1605    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5083
 
SalI G'TCGA_C

Cuts at:   1936    2425    1936 
   Size:        489    4594
 
SbfI CC_TGCA'GG

Cuts at:   2435    2435 
   Size:       5083
 
ScaI AGT'ACT

Cuts at:   3331    5043    3331 
   Size:       1712    3371
 
SfcI C'TryA_G

Cuts at:    826    1045    2431    3566    4247    4438     826 
   Size:        219    1386    1135     681     191    1471

  Fragments arranged by size: 

                1471    1386    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5083
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5083
 
SmaI CCC'GGG

Cuts at:   1872    2410    1872 
   Size:        538    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3187    4088    4350     820 
   Size:        197     926    1244     901     262    1553

  Fragments arranged by size: 

                1553    1244     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2874     493 
   Size:       2381    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5083
 
SphI G_CATG'C

Cuts at:   1132    2441    1132 
   Size:       1309    3774
 
SspI AAT'ATT

Cuts at:      5      52    3007       5 
   Size:         47    2955    2081

  Fragments arranged by size: 

                2955    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5083
 
StyI C'CwwG_G

Cuts at:    513    1065    2175    2181    2352    2379    2522     513 
   Size:        552    1110       6     171      27     143    3074

  Fragments arranged by size: 

                3074    1110     552     171     143      27       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3110    3127    3280    3465    3910     325 
   Size:       1255    1530      17     153     185     445    1498

  Fragments arranged by size: 

                1530    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2130    2367    3329 
   Size:        274      80      33      51    1443     153     237     962

Cuts at:   3329    5041      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443     962     274     237     153     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2324    2356    5043    5075    2324 
   Size:         32    2687      32    2332

  Fragments arranged by size: 

                2687    2332      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5083
 
XbaI T'CTAG_A

Cuts at:   2419    2419 
   Size:       5083
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5083
 
XmaI C'CCGG_G

Cuts at:   1870    2408    1870 
   Size:        538    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2198    3212    2198 
   Size:       1014    4069
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2891     276 
   Size:         53      83     186     936    1357    2468

  Fragments arranged by size: 

                2468    1357     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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