(Linear) (Six-base) MAPSORT of: KIEE5328.seq Check: 194 from: 1 to: 816 KIEE5328.orf With 182 enzymes: SgfI * January 29, 2014 11:55 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 272 816 Size: 272 544 AhdI GACnn_n'nnGTC Cuts at: 0 24 816 Size: 24 792 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 383 415 816 Size: 383 32 401 Fragments arranged by size: 401 383 32 AlwNI CAG_nnn'CTG Cuts at: 0 418 816 Size: 418 398 AvaI C'yCGr_G Cuts at: 0 84 816 Size: 84 732 BanI G'GyrC_C Cuts at: 0 43 816 Size: 43 773 BanII G_rGCy'C Cuts at: 0 698 816 Size: 698 118 BbeI G_GCGC'C Cuts at: 0 47 816 Size: 47 769 BbsI GAAGACnn'nnnn_ Cuts at: 0 261 816 Size: 261 555 BfrBI ATG'CAT Cuts at: 0 727 816 Size: 727 89 Bpu10I CC'TnA_GC Cuts at: 0 692 757 816 Size: 692 65 59 Fragments arranged by size: 692 65 59 BsaI GGTCTCn'nnnn_ Cuts at: 0 530 816 Size: 530 286 BsaBI GATnn'nnATC Cuts at: 0 606 816 Size: 606 210 BsaHI Gr'CG_yC Cuts at: 0 44 816 Size: 44 772 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 617 816 Size: 617 199 BseYI C'CCAG_C Cuts at: 0 127 678 816 Size: 127 551 138 Fragments arranged by size: 551 138 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 38 816 Size: 38 778 BsiEI CG_ry'CG Cuts at: 0 21 816 Size: 21 795 BsiHKAI G_wGCw'C Cuts at: 0 98 301 368 816 Size: 98 203 67 448 Fragments arranged by size: 448 203 98 67 Bsp1286I G_dGCh'C Cuts at: 0 98 301 368 698 816 Size: 98 203 67 330 118 Fragments arranged by size: 330 203 118 98 67 BspHI T'CATG_A Cuts at: 0 402 816 Size: 402 414 BstEII G'GTnAC_C Cuts at: 0 423 816 Size: 423 393 Bsu36I CC'TnA_GG Cuts at: 0 356 816 Size: 356 460 BtgI C'CryG_G Cuts at: 0 39 816 Size: 39 777 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 754 816 Size: 754 62 DraI TTT'AAA Cuts at: 0 497 816 Size: 497 319 EaeI y'GGCC_r Cuts at: 0 75 816 Size: 75 741 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 272 816 Size: 272 544 EcoNI CCTnn'n_nnAGG Cuts at: 0 355 816 Size: 355 461 EcoO109I rG'GnC_Cy Cuts at: 0 281 559 816 Size: 281 278 257 Fragments arranged by size: 281 278 257 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 285 664 816 Size: 285 379 152 Fragments arranged by size: 379 285 152 HaeII r_GCGC'y Cuts at: 0 39 47 816 Size: 39 8 769 Fragments arranged by size: 769 39 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 10 42 163 195 816 Size: 10 32 121 32 621 Fragments arranged by size: 621 121 32 32 10 KasI G'GCGC_C Cuts at: 0 43 816 Size: 43 773 MfeI C'AATT_G Cuts at: 0 291 816 Size: 291 525 MslI CAynn'nnrTG Cuts at: 0 722 816 Size: 722 94 MspA1I CmG'CkG Cuts at: 0 245 816 Size: 245 571 NarI GG'CG_CC Cuts at: 0 44 816 Size: 44 772 NsiI A_TGCA'T Cuts at: 0 729 816 Size: 729 87 PasI CC'CwG_GG Cuts at: 0 160 556 816 Size: 160 396 260 Fragments arranged by size: 396 260 160 PfoI T'CCnGG_A Cuts at: 0 344 816 Size: 344 472 PpuMI rG'GwC_Cy Cuts at: 0 281 816 Size: 281 535 SfoI GGC'GCC Cuts at: 0 45 816 Size: 45 771 SmaI CCC'GGG Cuts at: 0 86 816 Size: 86 730 SspI AAT'ATT Cuts at: 0 666 816 Size: 666 150 StyI C'CwwG_G Cuts at: 0 47 816 Size: 47 769 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 630 816 Size: 630 186 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 291 323 379 411 816 Size: 291 32 56 32 405 Fragments arranged by size: 405 291 56 32 32 XmaI C'CCGG_G Cuts at: 0 84 816 Size: 84 732 Enzymes that do cut: AcuI AhdI AloI AlwNI AvaI BanI BanII BbeI BbsI BfrBI Bpu10I BsaI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BstEII Bsu36I BtgI BtgZI DraI EaeI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MfeI MslI MspA1I NarI NsiI PasI PfoI PpuMI SfoI SmaI SspI StyI TaqII TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I BtsI ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI