Restriction map for
pFN21AE1746
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I FbaI
FokI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 18 CGCG CG^CG
AflIII 682 ACATGT A^C[GA][CT]GT
AhdI 250 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 26 GGGCCC GGGCC^C
AspEI 250 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 26 GGGCCC G[GA]GC[CT]^C
BbvCI 39 CCTCAGC CCTCAGC(-5/-2)
BciVI 614 GTATCC GTATCC(6/5)
BmyI 26 GGGCCC G[AGT]GC[ACT]^C
BsgI 58 CTGCAC GTGCAG(16/14)
BsmAI 366 GTCTC GTCTC(1/5)
Bsp1286I 26 GGGCCC G[AGT]GC[ACT]^C
BspLU11I 682 ACATGT A^CATGT
BssKI 30 CCTGG ^CC[ACGT]GG
BstNI 30 CCTGG CC^[AT]GG
BstUI 18 CGCG CG^CG
DpnII 538 GATC ^GATC
Eam1105I 250 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 368 CTCTTC CTCTTC(1/4)
EcoRI 208 GAATTC G^AATTC
EcoRII 30 CCTGG ^CC[AT]GG
EcoT22I 423 ATGCAT ATGCA^T
HaeIII 27 GGCC GG^CC
HgaI 16 GACGC GACGC(5/10)
Ksp632I 368 CTCTTC CTCTTC(1/4)
MaeII 501 ACGT A^CGT
MboI 538 GATC ^GATC
MslI 640 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 30 CCTGG CC^[AT]GG
MvnI 18 CGCG CG^CG
NdeII 538 GATC ^GATC
NsiI 423 ATGCAT ATGCA^T
NspI 682 ACATGT [GA]CATG^[CT]
PleI 364 GAGTC GAGTC(4/5)
PvuII 78 CAGCTG CAG^CTG
Sau3AI 538 GATC ^GATC
ScrFI 30 CCTGG CC^[ACGT]GG
SmlI 361 CTTGAG C^T[CT][GA]AG
SspI 325 AATATT AAT^ATT
TaqI 453 TCGA T^CGA
TfiI 316 GAATC G^A[AT]TC
Tsp45I 719 GTGAC ^GT[GC]AC
TthHB8I 453 TCGA T^CGA
XcmI 90 CCAACAAATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 201 AAATTC [GA]^AATT[CT]
208 GAATTC
AluI 79 AGCT AG^CT
216 AGCT
ApoI 201 AAATTC [GA]^AATT[CT]
208 GAATTC
AvaII 36 GGTCC G^G[AT]CC
159 GGTCC
BbvI 623 GCAGC GCAGC(8/12)
57 GCTGC
BsaJI 29 CCCTGG C^C[ACGT][ACGT]GG
30 CCTGGG
BsiYI 39 CCTCAGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
619 CCAAGCAGCGG
BslI 39 CCTCAGCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
619 CCAAGCAGCGG
BsrDI 140 GCAATG GCAATG(2/0)
492 GCAATG
BsrI 195 ACTGG ACTGG(1/-1)
512 CCAGT
Cac8I 24 GCGGGC GC[ACGT]^[ACGT]GC
53 GCCTGC
DdeI 40 CTCAG C^T[ACGT]AG
565 CTTAG
DraI 150 TTTAAA TTT^AAA
229 TTTAAA
FauI 19 GCGGG CCCGC(4/6)
24 GCGGG
Fnu4HI 57 GCTGC GC^[ACGT]GC
623 GCAGC
HinfI 316 GAATC G^A[ACGT]TC
364 GAGTC
ItaI 57 GCTGC GC^[ACGT]GC
623 GCAGC
MspA1I 78 CAGCTG C[AC]G^C[GT]G
624 CAGCGG
MwoI 19 GCGGGGCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
44 GCGTGGTCTGC
NlaIV 26 GGGCCC GG[ACGT]^[ACGT]CC
35 GGGTCC
PpuMI 35 GGGTCCT [GA]G^G[AT]CC[CT]
158 AGGTCCT
TseI 57 GCTGC G^C[AT]GC
623 GCAGC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AfaI Cfr13I DraII EcoO109I
MaeIII MboII MnlI MseI NlaIII
RsaI Sau96I Tru9I Tsp509I TspRI