(Circular) (Six-base) MAPSORT of: pFN21AE1746.seq Check: 9864 from: 1 to: 5455 pFN21AE1746 5455 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3265 278 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2798 1937 Size: 861 4594 Acc65I G'GTAC_C Cuts at: 2776 2776 Size: 5455 AclI AA'CG_TT Cuts at: 1563 3582 3955 1563 Size: 2019 373 3063 Fragments arranged by size: 3063 2019 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3518 4902 5170 1632 Size: 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5258 AflIII A'CryG_T Cuts at: 1203 2687 4354 5331 1203 Size: 1484 1667 977 1327 Fragments arranged by size: 1667 1484 1327 977 AgeI A'CCGG_T Cuts at: 4286 4286 Size: 5455 AhdI GACnn_n'nnGTC Cuts at: 2260 4184 2260 Size: 1924 3531 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5455 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5455 AlwNI CAG_nnn'CTG Cuts at: 3198 4770 3198 Size: 1572 3883 ApaI G_GGCC'C Cuts at: 2035 2035 Size: 5455 ApaLI G'TGCA_C Cuts at: 3512 4668 3512 Size: 1156 4299 ApoI r'AATT_y Cuts at: 1493 2206 2213 2764 2990 3142 5251 1493 Size: 713 7 551 226 152 2109 1697 Fragments arranged by size: 2109 1697 713 551 226 152 7 AseI AT'TA_AT Cuts at: 160 1032 4009 160 Size: 872 2977 1606 Fragments arranged by size: 2977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 AvaI C'yCGr_G Cuts at: 1870 1943 2780 1870 Size: 73 837 4545 Fragments arranged by size: 4545 837 73 BamHI G'GATC_C Cuts at: 2785 3152 2785 Size: 367 5088 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2776 4231 618 Size: 325 217 627 989 1455 1842 Fragments arranged by size: 1842 1455 989 627 325 217 BanII G_rGCy'C Cuts at: 1391 2035 2774 1391 Size: 644 739 4072 Fragments arranged by size: 4072 739 644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4484 BbvCI CC'TCA_GC Cuts at: 2045 2045 Size: 5455 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3646 3680 1903 Size: 34 1709 34 3678 Fragments arranged by size: 3678 1709 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2630 3347 4557 2630 Size: 717 1210 3528 Fragments arranged by size: 3528 1210 717 BclI T'GATC_A Cuts at: 1548 1548 Size: 5455 BfrBI ATG'CAT Cuts at: 2430 2430 Size: 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4066 136 Size: 107 122 71 631 605 2394 1525 Fragments arranged by size: 2394 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5450 5450 Size: 5455 BlpI GC'TnA_GC Cuts at: 2872 2872 Size: 5455 Bme1580I G_kGCm'C Cuts at: 1790 2035 3516 4672 1790 Size: 245 1481 1156 2573 Fragments arranged by size: 2573 1481 1156 245 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5455 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3186 4144 442 Size: 416 53 83 399 24 1769 958 1753 Fragments arranged by size: 1769 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5455 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4115 1239 Size: 2876 2579 Bpu10I CC'TnA_GC Cuts at: 2045 2045 Size: 5455 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 3580 4445 4743 2387 Size: 1193 865 298 3099 Fragments arranged by size: 3099 1193 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4118 882 Size: 617 2619 2219 Fragments arranged by size: 2619 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3246 493 Size: 711 2042 2702 Fragments arranged by size: 2702 2042 711 BsaBI GATnn'nnATC Cuts at: 5449 5449 Size: 5455 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3262 3644 4279 275 Size: 53 83 186 936 1729 382 635 1451 Fragments arranged by size: 1729 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3888 4286 4560 4707 5118 3888 Size: 398 274 147 411 4225 Fragments arranged by size: 4225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5230 Fragments arranged by size: 5230 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5455 BseYI C'CCAG_C Cuts at: 2012 4658 5154 2012 Size: 2646 496 2313 Fragments arranged by size: 2646 2313 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 1163 Size: 573 312 4570 Fragments arranged by size: 4570 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3666 4694 1540 Size: 275 186 904 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3516 3601 4672 3516 Size: 85 1071 4299 Fragments arranged by size: 4299 1071 85 BsmI GAATG_Cn' Cuts at: 2966 3059 2966 Size: 93 5362 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2774 3516 3601 4672 1391 Size: 399 245 739 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 739 399 245 85 BspEI T'CCGG_A Cuts at: 5118 5118 Size: 5455 BspHI T'CATG_A Cuts at: 3344 5074 3344 Size: 1730 3725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2810 844 Size: 1966 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3342 1394 Size: 429 1519 3507 Fragments arranged by size: 3507 1519 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2152 2504 3950 4124 65 Size: 1183 904 352 1446 174 1396 Fragments arranged by size: 1446 1396 1183 904 352 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4099 4286 1700 Size: 110 2289 187 2869 Fragments arranged by size: 2869 2289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5455 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5455 BssSI C'ACGA_G Cuts at: 3515 4527 3515 Size: 1012 4443 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5455 BstBI TT'CG_AA Cuts at: 4259 4259 Size: 5455 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5455 BstYI r'GATC_y Cuts at: 1972 2785 3152 3537 3554 5006 5092 5104 5450 Size: 813 367 385 17 1452 86 12 346 Cuts at: 5450 1972 Size: 1977 Fragments arranged by size: 1977 1452 813 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3964 Fragments arranged by size: 3964 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4716 Fragments arranged by size: 4716 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2971 3765 3785 2065 Size: 906 794 20 3735 Fragments arranged by size: 3735 906 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5420 DraI TTT'AAA Cuts at: 2157 2236 2759 2157 Size: 79 523 4853 Fragments arranged by size: 4853 523 79 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4462 809 Size: 507 3146 1802 Fragments arranged by size: 3146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2902 3791 5420 Size: 54 1425 50 275 18 1072 889 1629 Cuts at: 5420 8 Size: 43 Fragments arranged by size: 1629 1425 1072 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2902 1537 Size: 275 1090 4090 Fragments arranged by size: 4090 1090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 3385 1353 Size: 1026 1006 3423 Fragments arranged by size: 3423 1026 1006 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4042 4416 4562 387 Size: 1064 138 2453 374 146 1280 Fragments arranged by size: 2453 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3518 4115 4902 5170 1239 Size: 393 1886 597 787 268 1524 Fragments arranged by size: 1886 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2032 2041 2164 2032 Size: 9 123 5323 Fragments arranged by size: 5323 123 9 EcoRI G'AATT_C Cuts at: 2213 2764 3142 2213 Size: 551 378 4526 Fragments arranged by size: 4526 551 378 FspI TGC'GCA Cuts at: 3961 3961 Size: 5455 HaeII r_GCGC'y Cuts at: 1678 4602 1678 Size: 2924 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 4096 4128 4170 Size: 32 135 32 674 32 1818 32 42 Cuts at: 4170 4202 1373 Size: 32 2626 Fragments arranged by size: 2626 1818 674 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2799 3045 669 Size: 988 281 861 246 3079 Fragments arranged by size: 3079 988 861 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5455 HpaI GTT'AAC Cuts at: 3045 3045 Size: 5455 KpnI G_GTAC'C Cuts at: 2780 2780 Size: 5455 MfeI C'AATT_G Cuts at: 3054 3054 Size: 5455 MluI A'CGCG_T Cuts at: 5331 5331 Size: 5455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4569 4753 1305 Size: 3264 184 2007 Fragments arranged by size: 3264 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3633 Fragments arranged by size: 3633 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2649 3413 3772 3931 518 Size: 648 1483 764 359 159 2042 Fragments arranged by size: 2042 1483 764 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2631 2871 3548 4696 4941 1606 Size: 479 546 240 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 546 479 245 240 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3964 Fragments arranged by size: 3964 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5455 NotI GC'GGCC_GC Cuts at: 2902 2902 Size: 5455 NsiI A_TGCA'T Cuts at: 2432 2432 Size: 5455 NspI r_CATG'y Cuts at: 1132 1229 2691 2813 4358 1132 Size: 97 1462 122 1545 2229 Fragments arranged by size: 2229 1545 1462 122 97 PasI CC'CwG_GG Cuts at: 1348 2035 1348 Size: 687 4768 PciI A'CATG_T Cuts at: 2687 4354 2687 Size: 1667 3788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5455 PmeI GTTT'AAAC Cuts at: 2759 2759 Size: 5455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3524 3556 5037 5069 3524 Size: 32 1481 32 3910 Fragments arranged by size: 3910 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2164 2041 Size: 123 5332 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5455 PsiI TTA'TAA Cuts at: 2468 3025 2468 Size: 557 4898 PspOMI G'GGCC_C Cuts at: 2031 2031 Size: 5455 PstI C_TGCA'G Cuts at: 830 2807 830 Size: 1977 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5455 PvuII CAG'CTG Cuts at: 2085 2085 Size: 5455 SalI G'TCGA_C Cuts at: 1936 2797 1936 Size: 861 4594 SbfI CC_TGCA'GG Cuts at: 2807 2807 Size: 5455 ScaI AGT'ACT Cuts at: 3703 5415 3703 Size: 1712 3743 SfcI C'TryA_G Cuts at: 826 1045 2803 3938 4619 4810 826 Size: 219 1758 1135 681 191 1471 Fragments arranged by size: 1758 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5455 SmaI CCC'GGG Cuts at: 1872 2782 1872 Size: 910 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 3559 4460 4722 820 Size: 197 926 423 1193 901 262 1553 Fragments arranged by size: 1553 1193 926 901 423 262 197 SnaBI TAC'GTA Cuts at: 493 3246 493 Size: 2753 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5455 SphI G_CATG'C Cuts at: 1132 2813 1132 Size: 1681 3774 SspI AAT'ATT Cuts at: 5 52 2332 3379 5 Size: 47 2280 1047 2081 Fragments arranged by size: 2280 2081 1047 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5455 StyI C'CwwG_G Cuts at: 513 1065 2004 2894 513 Size: 552 939 890 3074 Fragments arranged by size: 3074 939 890 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3482 3499 3652 3837 4282 325 Size: 1255 1902 17 153 185 445 1498 Fragments arranged by size: 1902 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2564 3701 Size: 274 80 33 51 1443 495 92 1137 Cuts at: 3701 5413 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1137 495 274 138 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5415 5447 5415 Size: 32 5423 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5455 XbaI T'CTAG_A Cuts at: 2791 2791 Size: 5455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2102 2102 Size: 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5455 XmaI C'CCGG_G Cuts at: 1870 2780 1870 Size: 910 4545 XmnI GAAnn'nnTTC Cuts at: 3584 3584 Size: 5455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3263 276 Size: 53 83 186 936 1729 2468 Fragments arranged by size: 2468 1729 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI