################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI Aor51HI ApaI AscI Asp700I Asp718I AvaI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BclI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsoBI BspDI BspEI BsrDI BssHII BstAPI BstEII BstPI BstUI CelII ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoT22I FbaI FseI FspI HgaI HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcyI 1951 GGCGCC G[GA]^CG[CT]C AflII 1321 CTTAAG C^TTAAG AhdI 505 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 413 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 346 CCTAAGG CC^T[ACGT]AGG AspEI 505 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 351 GGATCC G^GATCC BanI 1951 GGCGCC G^G[CT][GA]CC BbeI 1951 GGCGCC GGCGC^C BciVI 1362 GGATAC GTATCC(6/5) BcnI 962 CCCGG CC^[GC]GG BfrI 1321 CTTAAG C^TTAAG BsaHI 1951 GGCGCC G[GA]^CG[CT]C BsaI 82 GAGACC GGTCTC(1/5) BseRI 1077 GAGGAG GAGGAG(10/8) BsmBI 126 CGTCTC CGTCTC(1/5) BsmI 1680 GAATGC GAATGC(1/-1) Bsp1407I 1867 TGTACA T^GTACA BspHI 473 TCATGA T^CATGA BspMI 2130 ACCTGC ACCTGC(4/8) BsrBI 1065 GAGCGG CCGCTC(-3/-3) BsrFI 1301 ACCGGC [GA]^CCGG[CT] BsrGI 1867 TGTACA T^GTACA Bst1107I 424 GTATAC GTA^TAC BstBI 1480 TTCGAA TT^CGAA BstZ17I 424 GTATAC GTA^TAC Bsu36I 346 CCTAAGG CC^T[ACGT]AGG CfoI 1952 GCGC GCG^C Cfr10I 1301 ACCGGC [GA]^CCGG[CT] DraI 73 TTTAAA TTT^AAA Eam1105I 505 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 346 CCTAAGG CC^T[ACGT]AGG EcoRI 1477 GAATTC G^AATTC EcoRV 101 GATATC GAT^ATC EcoT14I 24 CCTTGG C^C[AT][AT]GG HaeII 1951 GGCGCC [GA]GCGC^[CT] HhaI 1952 GCGC GCG^C Hin1I 1951 GGCGCC G[GA]^CG[CT]C HinP1I 1952 GCGC G^CGC HincII 996 GTTGAC GT[CT]^[GA]AC HindII 996 GTTGAC GT[CT]^[GA]AC HindIII 68 AAGCTT A^AGCTT KasI 1951 GGCGCC G^GCGCC NarI 1951 GGCGCC GG^CGCC NciI 962 CCCGG CC^[GC]GG NspV 1480 TTCGAA TT^CGAA PflMI 196 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 473 TCATGA T^CATGA ScaI 1932 AGTACT AGT^ACT SfoI 1951 GGCGCC GGC^GCC SfuI 1480 TTCGAA TT^CGAA SspBI 1867 TGTACA T^GTACA StyI 24 CCTTGG C^C[AT][AT]GG Van91I 196 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 1000 ACATGT A^C[GA][CT]GT 1870 ACATGT Alw44I 282 GTGCAC G^TGCAC 1146 GTGCAC ApaLI 282 GTGCAC G^TGCAC 1146 GTGCAC AseI 915 ATTAAT AT^TAAT 1779 ATTAAT AsnI 915 ATTAAT AT^TAAT 1779 ATTAAT BglII 332 AGATCT A^GATCT 947 AGATCT BspLU11I 1000 ACATGT A^CATGT 1870 ACATGT BssSI 1243 CTCGTG CACGAG(-5/-1) 2098 CTCGTG BstXI 524 CCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1885 CCAGAAACATGG Cac8I 633 GCAGGC GC[ACGT]^[ACGT]GC 1640 GCAGGC EarI 477 GAAGAG CTCTTC(1/4) 2060 GAAGAG FauI 1067 GCGGG CCCGC(4/6) 1987 GCGGG HapII 963 CCGG C^CGG 1302 CCGG HpaII 963 CCGG C^CGG 1302 CCGG Ksp632I 477 GAAGAG CTCTTC(1/4) 2060 GAAGAG MspI 963 CCGG C^CGG 1302 CCGG PshBI 915 ATTAAT AT^TAAT 1779 ATTAAT SacI 1195 GAGCTC GAGCT^C 1254 GAGCTC XbaI 97 TCTAGA T^CTAGA 1380 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AspHI AspI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII