(Circular) (Six-base) MAPSORT of: pFN21AE2075.seq Check: 1626 from: 1 to: 6922 pFN21AE2075 6922 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 2661 2755 4265 1937 Size: 493 231 94 1510 4594 Fragments arranged by size: 4594 1510 493 231 94 Acc65I G'GTAC_C Cuts at: 4243 4243 Size: 6922 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3929 4985 6369 6637 1632 Size: 2297 1056 1384 268 1917 Fragments arranged by size: 2297 1917 1384 1056 268 AflII C'TTAA_G Cuts at: 820 1017 3326 820 Size: 197 2309 4416 Fragments arranged by size: 4416 2309 197 AflIII A'CryG_T Cuts at: 1203 3005 3875 5821 6798 1203 Size: 1802 870 1946 977 1327 Fragments arranged by size: 1946 1802 1327 977 870 AgeI A'CCGG_T Cuts at: 5753 5753 Size: 6922 AhdI GACnn_n'nnGTC Cuts at: 2515 5651 2515 Size: 3136 3786 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6922 AlwNI CAG_nnn'CTG Cuts at: 2423 4665 6237 2423 Size: 2242 1572 3108 Fragments arranged by size: 3108 2242 1572 ApaLI G'TGCA_C Cuts at: 2287 3151 4979 6135 2287 Size: 864 1828 1156 3074 Fragments arranged by size: 3074 1828 1156 864 ApoI r'AATT_y Cuts at: 1493 2492 2865 3482 4231 4457 4609 6718 1493 Size: 999 373 617 749 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 749 617 373 226 152 AseI AT'TA_AT Cuts at: 160 1032 2921 3785 5476 160 Size: 872 1889 864 1691 1606 Fragments arranged by size: 1889 1691 1606 872 864 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 4247 1870 Size: 73 2304 4545 Fragments arranged by size: 4545 2304 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3534 3567 3534 Size: 33 6889 BamHI G'GATC_C Cuts at: 2356 4252 4619 2356 Size: 1896 367 4659 Fragments arranged by size: 4659 1896 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3956 4243 5698 618 Size: 325 217 627 2169 287 1455 1842 Fragments arranged by size: 2169 1842 1455 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 3119 3204 3263 4241 1391 Size: 1728 85 59 978 4072 Fragments arranged by size: 4072 1728 978 85 59 BbeI G_GCGC'C Cuts at: 3960 3960 Size: 6922 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5951 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5113 5147 1903 Size: 34 50 34 3092 34 3678 Fragments arranged by size: 3678 3092 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3361 4814 6024 3361 Size: 1453 1210 4259 Fragments arranged by size: 4259 1453 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6922 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5533 136 Size: 107 122 71 631 605 3861 1525 Fragments arranged by size: 3861 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2337 2952 6917 2337 Size: 615 3965 2342 Fragments arranged by size: 3965 2342 615 BlpI GC'TnA_GC Cuts at: 4339 4339 Size: 6922 Bme1580I G_kGCm'C Cuts at: 1790 2291 2628 3155 4983 6139 1790 Size: 501 337 527 1828 1156 2573 Fragments arranged by size: 2573 1828 1156 527 501 337 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6922 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3973 4653 5611 Size: 416 53 83 399 24 2556 680 958 Cuts at: 5611 442 Size: 1753 Fragments arranged by size: 2556 1753 958 680 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6922 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2359 2397 2492 3223 2359 Size: 38 95 731 6058 Fragments arranged by size: 6058 731 95 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2468 2519 2748 2797 3064 3332 3365 Size: 1166 63 51 229 49 267 268 33 Cuts at: 3365 3596 5582 1239 Size: 231 1986 2579 Fragments arranged by size: 2579 1986 1166 268 267 231 229 63 51 49 33 Bpu10I CC'TnA_GC Cuts at: 3113 4095 3113 Size: 982 5940 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2055 3628 5047 5912 6210 2055 Size: 1573 1419 865 298 2767 Fragments arranged by size: 2767 1573 1419 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 5585 882 Size: 617 582 3504 2219 Fragments arranged by size: 3504 2219 617 582 BsaAI yAC'GTr Cuts at: 493 1204 4713 493 Size: 711 3509 2702 Fragments arranged by size: 3509 2702 711 BsaBI GATnn'nnATC Cuts at: 6916 6916 Size: 6922 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3957 4729 5111 5746 Size: 53 83 186 936 2424 772 382 635 Cuts at: 5746 275 Size: 1451 Fragments arranged by size: 2424 1451 936 772 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5355 5753 6027 6174 6585 5355 Size: 398 274 147 411 5692 Fragments arranged by size: 5692 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2536 2566 2979 3009 3843 Size: 30 165 30 1133 30 413 30 834 Cuts at: 3843 3873 3921 3951 1178 Size: 30 48 30 4149 Fragments arranged by size: 4149 1133 834 413 165 48 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3097 1186 Size: 1911 5011 BseYI C'CCAG_C Cuts at: 2390 6125 6621 2390 Size: 3735 496 2691 Fragments arranged by size: 3735 2691 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6349 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4372 5133 6161 1540 Size: 275 186 2371 761 1028 2301 Fragments arranged by size: 2371 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2291 3155 3204 3263 4110 4983 5068 6139 2291 Size: 864 49 59 847 873 85 1071 3074 Fragments arranged by size: 3074 1071 873 864 847 85 59 49 BsmI GAATG_Cn' Cuts at: 3691 4433 4526 3691 Size: 742 93 6087 Fragments arranged by size: 6087 742 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2137 2137 Size: 6922 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2291 2628 3119 3155 3204 3263 4110 Size: 399 501 337 491 36 49 59 847 Cuts at: 4110 4241 4983 5068 6139 1391 Size: 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 847 742 501 491 399 337 131 85 59 49 36 BspEI T'CCGG_A Cuts at: 6585 6585 Size: 6922 BspHI T'CATG_A Cuts at: 2478 4811 6541 2478 Size: 2333 1730 2859 Fragments arranged by size: 2859 2333 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4144 4277 844 Size: 3300 133 3489 Fragments arranged by size: 3489 3300 133 BsrBI CCG'CTC Cuts at: 1394 1823 3072 4809 1394 Size: 429 1249 1737 3507 Fragments arranged by size: 3507 1737 1249 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5417 5591 65 Size: 1183 4169 174 1396 Fragments arranged by size: 4169 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3306 5566 5753 1700 Size: 110 1496 2260 187 2869 Fragments arranged by size: 2869 2260 1496 187 110 BsrGI T'GTAC_A Cuts at: 96 3872 96 Size: 3776 3146 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6922 BssSI C'ACGA_G Cuts at: 3248 4103 4982 5994 3248 Size: 855 879 1012 4176 Fragments arranged by size: 4176 1012 879 855 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6922 BstBI TT'CG_AA Cuts at: 3486 5726 3486 Size: 2240 4682 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6922 BstXI CCAn_nnnn'nTGG Cuts at: 2536 3897 2536 Size: 1361 5561 BstYI r'GATC_y Cuts at: 1972 2337 2356 2952 3220 4252 4619 5004 5021 Size: 365 19 596 268 1032 367 385 17 Cuts at: 5021 6473 6559 6571 6917 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1032 596 385 367 365 346 268 86 19 17 12 BstZ17I GTA'TAC Cuts at: 2431 2431 Size: 6922 Bsu36I CC'TnA_GG Cuts at: 2352 2352 Size: 6922 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6370 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6183 Fragments arranged by size: 6183 683 56 BtsI GCAGTG_nn' Cuts at: 4438 5232 5252 4438 Size: 794 20 6108 Fragments arranged by size: 6108 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6922 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6887 DraI TTT'AAA Cuts at: 2080 4226 2080 Size: 2146 4776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5929 809 Size: 507 4613 1802 Fragments arranged by size: 4613 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4369 5258 6887 Size: 54 1425 50 275 18 2539 889 1629 Cuts at: 6887 8 Size: 43 Fragments arranged by size: 2539 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4369 1537 Size: 275 2557 4090 Fragments arranged by size: 4090 2557 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2477 4060 4852 1353 Size: 1124 1583 792 3423 Fragments arranged by size: 3423 1583 1124 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5509 5883 6029 387 Size: 1064 138 3920 374 146 1280 Fragments arranged by size: 3920 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3202 3261 3202 Size: 59 6863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2468 2519 2748 2797 3064 3332 Size: 393 773 63 51 229 49 267 268 Cuts at: 3332 3365 3596 3929 4985 5582 6369 6637 1239 Size: 33 231 333 1056 597 787 268 1524 Fragments arranged by size: 1524 1056 787 773 597 393 333 268 268 267 231 229 63 51 49 33 EcoRI G'AATT_C Cuts at: 3482 4231 4609 3482 Size: 749 378 5795 Fragments arranged by size: 5795 749 378 EcoRV GAT'ATC Cuts at: 2108 2108 Size: 6922 FspI TGC'GCA Cuts at: 5428 5428 Size: 6922 HaeII r_GCGC'y Cuts at: 1678 3960 6069 1678 Size: 2282 2109 2531 Fragments arranged by size: 2531 2282 2109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2182 2214 2236 Size: 32 135 32 545 32 33 32 22 Cuts at: 2236 2268 2501 2533 2962 2979 2994 3011 3365 Size: 32 233 32 429 17 15 17 354 Cuts at: 3365 3397 3843 3875 5563 5595 5637 5669 1373 Size: 32 446 32 1688 32 42 32 2626 Fragments arranged by size: 2626 1688 545 446 429 354 233 135 42 33 32 32 32 32 32 32 32 32 32 32 22 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3003 4266 4512 669 Size: 988 281 1065 1263 246 3079 Fragments arranged by size: 3079 1263 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 2073 748 Size: 1325 5597 HpaI GTT'AAC Cuts at: 4512 4512 Size: 6922 KasI G'GCGC_C Cuts at: 3956 3956 Size: 6922 KpnI G_GTAC'C Cuts at: 4247 4247 Size: 6922 MfeI C'AATT_G Cuts at: 4521 4521 Size: 6922 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6036 6220 1305 Size: 4731 184 2007 Fragments arranged by size: 4731 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5100 Fragments arranged by size: 5100 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2373 2607 3471 4145 4880 5239 5398 Size: 648 1207 234 864 674 735 359 159 Cuts at: 5398 518 Size: 2042 Fragments arranged by size: 2042 1207 864 735 674 648 359 234 159 MspA1I CmG'CkG Cuts at: 1606 4338 5015 6163 6408 1606 Size: 2732 677 1148 245 2120 Fragments arranged by size: 2732 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3957 3957 Size: 6922 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6370 NdeI CA'TA_TG Cuts at: 387 387 Size: 6922 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6922 NotI GC'GGCC_GC Cuts at: 4369 4369 Size: 6922 NspI r_CATG'y Cuts at: 1132 1229 3009 3606 3879 4280 5825 1132 Size: 97 1780 597 273 401 1545 2229 Fragments arranged by size: 2229 1780 1545 597 401 273 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6922 PciI A'CATG_T Cuts at: 3005 3875 5821 3005 Size: 870 1946 4106 Fragments arranged by size: 4106 1946 870 PflMI CCAn_nnn'nTGG Cuts at: 1108 2207 1108 Size: 1099 5823 PfoI T'CCnGG_A Cuts at: 3041 3573 3041 Size: 532 6390 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2261 2293 3125 3157 4991 5023 6504 6536 2261 Size: 32 832 32 1834 32 1481 32 2647 Fragments arranged by size: 2647 1834 1481 832 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6922 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PstI C_TGCA'G Cuts at: 830 4274 830 Size: 3444 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 SacI G_AGCT'C Cuts at: 3204 3263 3204 Size: 59 6863 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SbfI CC_TGCA'GG Cuts at: 4274 4274 Size: 6922 ScaI AGT'ACT Cuts at: 3939 5170 6882 3939 Size: 1231 1712 3979 Fragments arranged by size: 3979 1712 1231 SfcI C'TryA_G Cuts at: 826 1045 2804 3668 3948 4270 5405 6086 6277 Size: 219 1759 864 280 322 1135 681 191 Cuts at: 6277 826 Size: 1471 Fragments arranged by size: 1759 1471 1135 864 681 322 280 219 191 SfoI GGC'GCC Cuts at: 3958 3958 Size: 6922 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 4249 1872 Size: 2377 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2070 3326 3607 5026 5927 6189 Size: 197 926 127 1256 281 1419 901 262 Cuts at: 6189 820 Size: 1553 Fragments arranged by size: 1553 1419 1256 926 901 281 262 197 127 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 4280 1132 Size: 3148 3774 SspI AAT'ATT Cuts at: 5 52 4846 5 Size: 47 4794 2081 Fragments arranged by size: 4794 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6922 StyI C'CwwG_G Cuts at: 513 1065 2029 4361 513 Size: 552 964 2332 3074 Fragments arranged by size: 3074 2332 964 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2686 4949 4966 5119 5304 5749 325 Size: 1255 1106 2263 17 153 185 445 1498 Fragments arranged by size: 2263 1498 1255 1106 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3872 3937 5168 Size: 274 80 33 51 1443 1895 65 1231 Cuts at: 5168 6880 96 Size: 1712 138 Fragments arranged by size: 1895 1712 1443 1231 274 138 80 65 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2316 2348 2856 2888 3180 3212 6882 6914 2316 Size: 32 508 32 292 32 3670 32 2324 Fragments arranged by size: 3670 2324 508 292 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2129 2993 3857 1316 Size: 813 864 864 4381 Fragments arranged by size: 4381 864 864 813 XbaI T'CTAG_A Cuts at: 2102 3385 4258 2102 Size: 1283 873 4766 Fragments arranged by size: 4766 1283 873 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2175 2772 3039 2175 Size: 597 267 6058 Fragments arranged by size: 6058 597 267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6922 XmaI C'CCGG_G Cuts at: 1870 4247 1870 Size: 2377 4545 XmnI GAAnn'nnTTC Cuts at: 5051 5051 Size: 6922 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BsiWI DraIII EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI