################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRII EcoT14I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MamI MluI MluNI MroI MscI MspI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AocI 1093 CCTCAGG CC^T[ACGT]AGG AvaI 1718 CTCGAG C^[CT]CG[GA]G BanII 334 GAGCCC G[GA]GC[CT]^C BbsI 1039 GAAGAC GAAGAC(2/6) BclI 900 TGATCA T^GATCA BglII 1602 AGATCT A^GATCT BpmI 1015 CTGGAG CTGGAG(16/14) BpuAI 1039 GAAGAC GAAGAC(2/6) BsaAI 1304 CACGTA [CT]AC^GT[GA] BsgI 1376 GTGCAG GTGCAG(16/14) BsmAI 1132 GAGAC GTCTC(1/5) BsmI 1674 GCATTC GAATGC(1/-1) BsoBI 1718 CTCGAG C^[CT]CG[GA]G Bsp1407I 882 TGTACA T^GTACA BspLU11I 1286 ACATGT A^CATGT BsrGI 882 TGTACA T^GTACA BstXI 998 CCAAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1093 CCTCAGG CC^T[ACGT]AGG DraI 61 TTTAAA TTT^AAA DraII 1667 AGGTCCT [GA]G^G[ACGT]CC[CT] Eco81I 1093 CCTCAGG CC^T[ACGT]AGG EcoO109I 1667 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 351 GATATC GAT^ATC EcoT22I 105 ATGCAT ATGCA^T FbaI 900 TGATCA T^GATCA HindIII 642 AAGCTT A^AGCTT MaeII 1305 ACGT A^CGT MaeIII 1307 GTAAC ^GT[ACGT]AC MslI 1237 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1165 CAGCTG C[AC]G^C[GT]G NsiI 105 ATGCAT ATGCA^T NspI 1286 ACATGT [GA]CATG^[CT] PaeR7I 1718 CTCGAG C^TCGAG PpuMI 1667 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 1042 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1165 CAGCTG CAG^CTG ScaI 1061 AGTACT AGT^ACT SfcI 5 CTGTAG C^T[GA][CT]AG SspBI 882 TGTACA T^GTACA StuI 533 AGGCCT AGG^CCT XhoI 1718 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflII 918 CTTAAG C^TTAAG 1663 CTTAAG AflIII 1286 ACATGT A^C[GA][CT]GT 1303 ACACGT AlwI 703 GATCC GGATC(4/5) 1635 GATCC BbvI 1659 GCAGC GCAGC(8/12) 1011 GCTGC BfrI 918 CTTAAG C^TTAAG 1663 CTTAAG BstYI 1602 AGATCT [GA]^GATC[CT] 1634 AGATCC Fnu4HI 1011 GCTGC GC^[ACGT]GC 1659 GCAGC ItaI 1011 GCTGC GC^[ACGT]GC 1659 GCAGC MfeI 425 CAATTG C^AATTG 749 CAATTG MflI 1602 AGATCT [GA]^GATC[CT] 1634 AGATCC MunI 425 CAATTG C^AATTG 749 CAATTG MwoI 644 GCTTGAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 859 GCTTCTCTGGC NlaIII 784 CATG CATG^ 1287 CATG PleI 850 GACTC GAGTC(4/5) 985 GAGTC SapI 49 GAAGAGC GCTCTTC(1/4) 331 GAAGAGC SspI 1498 AATATT AAT^ATT 1768 AATATT TaqI 210 TCGA T^CGA 1719 TCGA TseI 1011 GCTGC G^C[AT]GC 1659 GCAGC TthHB8I 210 TCGA T^CGA 1719 TCGA XhoII 1602 AGATCT [GA]^GATC[CT] 1634 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccI AcsI AfaI AluI ApoI Asp700I AvaII BfaI BmyI Bsp1286I BsrI BstF5I Cfr13I DdeI DpnII EarI FokI HaeIII HinfI Ksp632I MaeI MboI MboII MnlI MseI NdeII RsaI Sau3AI Sau96I SfaNI SmlI TfiI Tru9I Tsp509I TspRI XmnI