(Circular) (Six-base) MAPSORT of: pFN21AE2018.seq Check: 711 from: 1 to: 6487 pFN21AE2018 6487 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4297 278 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2447 3055 3758 3830 1937 Size: 510 608 703 72 4594 Fragments arranged by size: 4594 703 608 510 72 Acc65I G'GTAC_C Cuts at: 3808 3808 Size: 6487 AclI AA'CG_TT Cuts at: 1563 4614 4987 1563 Size: 3051 373 3063 Fragments arranged by size: 3063 3051 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2006 2366 2712 3152 3786 4550 5934 6202 Size: 374 360 346 440 634 764 1384 268 Cuts at: 6202 1632 Size: 1917 Fragments arranged by size: 1917 1384 764 634 440 374 360 346 268 AflII C'TTAA_G Cuts at: 820 1017 2923 3668 820 Size: 197 1906 745 3639 Fragments arranged by size: 3639 1906 745 197 AflIII A'CryG_T Cuts at: 1203 3291 3308 5386 6363 1203 Size: 2088 17 2078 977 1327 Fragments arranged by size: 2088 2078 1327 977 17 AgeI A'CCGG_T Cuts at: 5318 5318 Size: 6487 AhdI GACnn_n'nnGTC Cuts at: 5216 5216 Size: 6487 AleI CACnn'nnGTG Cuts at: 1166 3246 1166 Size: 2080 4407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6487 AlwNI CAG_nnn'CTG Cuts at: 4230 5802 4230 Size: 1572 4915 ApaLI G'TGCA_C Cuts at: 4544 5700 4544 Size: 1156 5331 ApoI r'AATT_y Cuts at: 1493 2633 2822 3430 3796 4022 4174 6283 1493 Size: 1140 189 608 366 226 152 2109 1697 Fragments arranged by size: 2109 1697 1140 608 366 226 189 152 AseI AT'TA_AT Cuts at: 160 1032 5041 160 Size: 872 4009 1606 Fragments arranged by size: 4009 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 AvaI C'yCGr_G Cuts at: 1870 1943 3723 3812 1870 Size: 73 1780 89 4545 Fragments arranged by size: 4545 1780 89 73 BamHI G'GATC_C Cuts at: 3817 4184 3817 Size: 367 6120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3808 5263 618 Size: 325 217 627 2021 1455 1842 Fragments arranged by size: 2021 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2343 3806 1391 Size: 952 1463 4072 Fragments arranged by size: 4072 1463 952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3051 928 Size: 971 1152 4364 Fragments arranged by size: 4364 1152 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4678 4712 1903 Size: 34 2741 34 3678 Fragments arranged by size: 3678 2741 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4379 5589 4379 Size: 1210 5277 BclI T'GATC_A Cuts at: 1548 2905 1548 Size: 1357 5130 BfrBI ATG'CAT Cuts at: 2112 2112 Size: 6487 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5098 136 Size: 107 122 71 631 605 3426 1525 Fragments arranged by size: 3426 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3607 6482 3607 Size: 2875 3612 BlpI GC'TnA_GC Cuts at: 3904 3904 Size: 6487 Bme1580I G_kGCm'C Cuts at: 1790 2127 2961 4548 5704 1790 Size: 337 834 1587 1156 2573 Fragments arranged by size: 2573 1587 1156 834 337 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6487 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4218 5176 442 Size: 416 53 83 399 24 2801 958 1753 Fragments arranged by size: 2801 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6487 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3041 5147 1239 Size: 1802 2106 2579 Fragments arranged by size: 2579 2106 1802 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4612 5477 5775 4612 Size: 865 298 5324 Fragments arranged by size: 5324 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5150 882 Size: 617 3651 2219 Fragments arranged by size: 3651 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3311 4278 493 Size: 711 2107 967 2702 Fragments arranged by size: 2702 2107 967 711 BsaBI GATnn'nnATC Cuts at: 6481 6481 Size: 6487 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4294 4676 5311 275 Size: 53 83 186 936 2761 382 635 1451 Fragments arranged by size: 2761 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4920 5318 5592 5739 6150 4920 Size: 398 274 147 411 5257 Fragments arranged by size: 5257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3354 3384 1178 Size: 30 165 30 1951 30 4281 Fragments arranged by size: 4281 1951 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6487 BseYI C'CCAG_C Cuts at: 2099 5690 6186 2099 Size: 3591 496 2400 Fragments arranged by size: 3591 2400 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3402 1163 Size: 573 1666 4248 Fragments arranged by size: 4248 1666 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3937 4698 5726 1540 Size: 275 186 1936 761 1028 2301 Fragments arranged by size: 2301 1936 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4548 4633 5704 4548 Size: 85 1071 5331 Fragments arranged by size: 5331 1071 85 BsmI GAATG_Cn' Cuts at: 3679 3998 4091 3679 Size: 319 93 6075 Fragments arranged by size: 6075 319 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2343 2961 3806 4548 4633 5704 Size: 399 337 216 618 845 742 85 1071 Cuts at: 5704 1391 Size: 2174 Fragments arranged by size: 2174 1071 845 742 618 399 337 216 85 BspEI T'CCGG_A Cuts at: 6150 6150 Size: 6487 BspHI T'CATG_A Cuts at: 4376 6106 4376 Size: 1730 4757 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3842 844 Size: 2998 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4374 1394 Size: 429 2551 3507 Fragments arranged by size: 3507 2551 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4982 5156 65 Size: 1183 3734 174 1396 Fragments arranged by size: 3734 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5131 5318 1700 Size: 110 3321 187 2869 Fragments arranged by size: 3321 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2887 96 Size: 2791 3696 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6487 BssSI C'ACGA_G Cuts at: 4547 5559 4547 Size: 1012 5475 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6487 BstBI TT'CG_AA Cuts at: 5291 5291 Size: 6487 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6487 BstXI CCAn_nnnn'nTGG Cuts at: 3010 3010 Size: 6487 BstYI r'GATC_y Cuts at: 1972 3607 3639 3817 4184 4569 4586 6038 6124 Size: 1635 32 178 367 385 17 1452 86 Cuts at: 6124 6136 6482 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1635 1452 385 367 346 178 86 32 17 12 Bsu36I CC'TnA_GG Cuts at: 3099 3099 Size: 6487 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4996 Fragments arranged by size: 4996 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5748 Fragments arranged by size: 5748 683 56 BtsI GCAGTG_nn' Cuts at: 4003 4797 4817 4003 Size: 794 20 5673 Fragments arranged by size: 5673 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6487 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6452 DraI TTT'AAA Cuts at: 2068 3791 2068 Size: 1723 4764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5494 809 Size: 507 4178 1802 Fragments arranged by size: 4178 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3934 4823 6452 Size: 54 1425 50 275 18 2104 889 1629 Cuts at: 6452 8 Size: 43 Fragments arranged by size: 2104 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3934 1537 Size: 275 2122 4090 Fragments arranged by size: 4090 2122 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2067 2331 2475 2982 3630 4417 1353 Size: 696 18 264 144 507 648 787 3423 Fragments arranged by size: 3423 787 696 648 507 264 144 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5074 5448 5594 387 Size: 1064 138 3485 374 146 1280 Fragments arranged by size: 3485 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2006 2366 2712 3041 3152 3786 4550 Size: 393 374 360 346 329 111 634 764 Cuts at: 4550 5147 5934 6202 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 764 634 597 393 374 360 346 329 268 111 EcoO109I rG'GnC_Cy Cuts at: 3673 3673 Size: 6487 EcoRI G'AATT_C Cuts at: 3796 4174 3796 Size: 378 6109 EcoRV GAT'ATC Cuts at: 2358 2358 Size: 6487 FspI TGC'GCA Cuts at: 4993 4993 Size: 6487 HaeII r_GCGC'y Cuts at: 1678 5634 1678 Size: 3956 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 2770 2802 3624 Size: 32 135 32 459 32 707 32 822 Cuts at: 3624 3656 5128 5160 5202 5234 1373 Size: 32 1472 32 42 32 2626 Fragments arranged by size: 2626 1472 822 707 459 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3831 4077 669 Size: 988 281 1893 246 3079 Fragments arranged by size: 3079 1893 988 281 246 HindIII A'AGCT_T Cuts at: 748 2647 748 Size: 1899 4588 HpaI GTT'AAC Cuts at: 4077 4077 Size: 6487 KpnI G_GTAC'C Cuts at: 3812 3812 Size: 6487 MfeI C'AATT_G Cuts at: 2430 2754 4086 2430 Size: 324 1332 4831 Fragments arranged by size: 4831 1332 324 MluI A'CGCG_T Cuts at: 6363 6363 Size: 6487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3168 5601 5785 1305 Size: 1863 2433 184 2007 Fragments arranged by size: 2433 2007 1863 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4665 Fragments arranged by size: 4665 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3246 4445 4804 4963 518 Size: 648 2080 1199 359 159 2042 Fragments arranged by size: 2080 2042 1199 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3172 3903 4580 5728 5973 1606 Size: 1566 731 677 1148 245 2120 Fragments arranged by size: 2120 1566 1148 731 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4996 Fragments arranged by size: 4996 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6487 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6487 NotI GC'GGCC_GC Cuts at: 3934 3934 Size: 6487 NsiI A_TGCA'T Cuts at: 2114 2114 Size: 6487 NspI r_CATG'y Cuts at: 1132 1229 3295 3845 5390 1132 Size: 97 2066 550 1545 2229 Fragments arranged by size: 2229 2066 1545 550 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6487 PciI A'CATG_T Cuts at: 3291 5386 3291 Size: 2095 4392 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6487 PmeI GTTT'AAAC Cuts at: 3791 3791 Size: 6487 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4556 4588 6069 6101 4556 Size: 32 1481 32 4942 Fragments arranged by size: 4942 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3673 3673 Size: 6487 PshAI GACnn'nnGTC Cuts at: 1613 3051 1613 Size: 1438 5049 PsiI TTA'TAA Cuts at: 4057 4057 Size: 6487 PspXI vC'TCGA_Gb Cuts at: 3723 3723 Size: 6487 PstI C_TGCA'G Cuts at: 830 3839 830 Size: 3009 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6487 PvuII CAG'CTG Cuts at: 3172 3172 Size: 6487 SalI G'TCGA_C Cuts at: 1936 3829 1936 Size: 1893 4594 SapI GCTCTTCn'nnn_ Cuts at: 2049 2331 2049 Size: 282 6205 SbfI CC_TGCA'GG Cuts at: 3839 3839 Size: 6487 ScaI AGT'ACT Cuts at: 3068 4735 6447 3068 Size: 1667 1712 3108 Fragments arranged by size: 3108 1712 1667 SfcI C'TryA_G Cuts at: 826 1045 2010 3835 4970 5651 5842 826 Size: 219 965 1825 1135 681 191 1471 Fragments arranged by size: 1825 1471 1135 965 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6487 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6487 SmaI CCC'GGG Cuts at: 1872 3814 1872 Size: 1942 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2923 3668 3723 4591 5492 5754 Size: 197 926 980 745 55 868 901 262 Cuts at: 5754 820 Size: 1553 Fragments arranged by size: 1553 980 926 901 868 745 262 197 55 SnaBI TAC'GTA Cuts at: 493 4278 493 Size: 3785 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6487 SphI G_CATG'C Cuts at: 1132 3845 1132 Size: 2713 3774 SspI AAT'ATT Cuts at: 5 52 3505 3775 4411 5 Size: 47 3453 270 636 2081 Fragments arranged by size: 3453 2081 636 270 47 StuI AGG'CCT Cuts at: 1517 2540 1517 Size: 1023 5464 StyI C'CwwG_G Cuts at: 513 1065 2004 3926 513 Size: 552 939 1922 3074 Fragments arranged by size: 3074 1922 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4514 4531 4684 4869 5314 325 Size: 1255 2934 17 153 185 445 1498 Fragments arranged by size: 2934 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2887 3066 4733 Size: 274 80 33 51 1443 910 179 1667 Cuts at: 4733 6445 96 Size: 1712 138 Fragments arranged by size: 1712 1667 1443 910 274 179 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6447 6479 6447 Size: 32 6455 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6487 XbaI T'CTAG_A Cuts at: 3823 3823 Size: 6487 XhoI C'TCGA_G Cuts at: 1943 3723 1943 Size: 1780 4707 XmaI C'CCGG_G Cuts at: 1870 3812 1870 Size: 1942 4545 XmnI GAAnn'nnTTC Cuts at: 2148 3515 3714 4616 2148 Size: 1367 199 902 4019 Fragments arranged by size: 4019 1367 902 199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4295 276 Size: 53 83 186 936 2761 2468 Fragments arranged by size: 2761 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI