################################## # NO CUT ENZYMES ( 217 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AccII 73 CGCG CG^CG AfaI 164 GTAC GT^AC Asp700I 105 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BsaJI 86 CCCAGG C^C[ACGT][ACGT]GG BseRI 9 CTCCTC GAGGAG(10/8) BsiYI 51 CCGGCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 51 CCGGCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 123 GAATGC GAATGC(1/-1) BsrI 148 ACTGG ACTGG(1/-1) BssKI 87 CCAGG ^CC[ACGT]GG BstNI 87 CCAGG CC^[AT]GG BstUI 73 CGCG CG^CG EcoRII 87 CCAGG ^CC[AT]GG HaeIII 60 GGCC GG^CC HgaI 17 GCGTC GACGC(5/10) HinfI 65 GACTC G^A[ACGT]TC MspA1I 92 CAGCTG C[AC]G^C[GT]G MvaI 87 CCAGG CC^[AT]GG MvnI 73 CGCG CG^CG MwoI 8 GCTCCTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 133 CATG CATG^ NlaIV 7 GGCTCC GG[ACGT]^[ACGT]CC PleI 65 GACTC GAGTC(4/5) PvuII 92 CAGCTG CAG^CTG RsaI 164 GTAC GT^AC ScrFI 87 CCAGG CC^[ACGT]GG XmnI 105 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AciI 23 CCGC CCGC(-3/-1) 72 CCGC AluI 47 AGCT AG^CT 93 AGCT BbvI 91 GCAGC GCAGC(8/12) 94 GCTGC HapII 51 CCGG C^CGG 62 CCGG HpaII 51 CCGG C^CGG 62 CCGG MboII 30 GAAGA GAAGA(8/7) 157 TCTTC MspI 51 CCGG C^CGG 62 CCGG PstI 95 CTGCAG CTGCA^G 118 CTGCAG SfcI 95 CTGCAG C^T[GA][CT]AG 118 CTGCAG TseI 91 GCAGC G^C[AT]GC 94 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### BpmI Fnu4HI ItaI MnlI