Restriction map for
pFN21AE2195
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp718I AspEI AvaII AviII
AvrII BbrPI BbvCI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 511 ACGCGT A^C[GA][CT]GT
AlwNI 122 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 255 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1023 GGATCC G^GATCC
BfaI 656 CTAG C^TAG
BglI 472 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1016 AGATCT A^GATCT
BpmI 119 CTCCAG CTGGAG(16/14)
BseRI 426 CTCCTC GAGGAG(10/8)
BsmBI 835 GAGACG CGTCTC(1/5)
BsmI 970 GAATGC GAATGC(1/-1)
Bsp1407I 1223 TGTACA T^GTACA
BspMI 1037 ACCTGC ACCTGC(4/8)
BsrGI 1223 TGTACA T^GTACA
BstBI 1061 TTCGAA TT^CGAA
BstF5I 27 GGATG GGATG(2/0)
BstXI 443 CCAGCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 761 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1148 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 660 CTCTTC CTCTTC(1/4)
EcoO109I 761 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 27 GGATG GGATG(9/13)
HgaI 510 GACGC GACGC(5/10)
Ksp632I 660 CTCTTC CTCTTC(1/4)
MaeI 656 CTAG C^TAG
MaeII 616 ACGT A^CGT
MaeIII 730 GTCAC ^GT[ACGT]AC
MluI 511 ACGCGT A^CGCGT
MspA1I 774 CCGCTG C[AC]G^C[GT]G
NcoI 449 CCATGG C^CATGG
NspI 68 GCATGT [GA]CATG^[CT]
NspV 1061 TTCGAA TT^CGAA
PflFI 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1091 GACTC GAGTC(4/5)
PstI 1110 CTGCAG CTGCA^G
SapI 659 GCTCTTC GCTCTTC(1/4)
SexAI 296 ACCTGGT A^CC[AT]GGT
SfcI 1110 CTGCAG C^T[GA][CT]AG
SfuI 1061 TTCGAA TT^CGAA
SspBI 1223 TGTACA T^GTACA
TfiI 1232 GAATC G^A[AT]TC
Tsp45I 730 GTCAC ^GT[GC]AC
Tsp509I 1103 AATT ^AATT
Tth111I 295 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 255 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 1104 ATTAAT AT^TAAT
1216 ATTAAT
AsnI 1104 ATTAAT AT^TAAT
1216 ATTAAT
BanII 421 GGGCTC G[GA]GC[CT]^C
712 GGGCTC
BbsI 507 GAAGAC GAAGAC(2/6)
1088 GAAGAC
BpuAI 507 GAAGAC GAAGAC(2/6)
1088 GAAGAC
BsaI 7 GAGACC GGTCTC(1/5)
884 GGTCTC
BsiEI 323 CGGTCG CG[GA][CT]^CG
830 CGACCG
BsrBI 889 CCGCTC CCGCTC(-3/-3)
460 GAGCGG
BsrFI 230 ACCGGC [GA]^CCGG[CT]
573 GCCGGT
BssSI 214 CTCGTG CACGAG(-5/-1)
1204 CTCGTG
Cfr10I 230 ACCGGC [GA]^CCGG[CT]
573 GCCGGT
HinfI 1091 GACTC G^A[ACGT]TC
1232 GAATC
PflMI 121 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1014 CCAGATCTTGG
PshBI 1104 ATTAAT AT^TAAT
1216 ATTAAT
SmaI 11 CCCGGG CCC^GGG
610 CCCGGG
Van91I 121 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1014 CCAGATCTTGG
XcmI 755 CCATTAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
842 CCATTCAGCTTATGG
XmaCI 11 CCCGGG C^CCGGG
610 CCCGGG
XmaI 11 CCCGGG C^CCGGG
610 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI BalI BanI
BbeI BbvI BcgI BcnI BmyI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI StyI
TaqI Tru9I TseI TspRI TthHB8I
XhoII