(Circular) (Six-base) MAPSORT of: pFN21AE2195.seq Check: 6891 from: 1 to: 5959 pFN21AE2195 5959 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2516 4858 5835 1203 Size: 1313 2342 977 1327 Fragments arranged by size: 2342 1327 1313 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2514 1184 Size: 1330 4629 AlwNI CAG_nnn'CTG Cuts at: 2132 3702 5274 2132 Size: 1570 1572 2817 Fragments arranged by size: 2817 1572 1570 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3110 3222 4513 160 Size: 872 2078 112 1291 1606 Fragments arranged by size: 2078 1606 1291 872 112 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2084 2615 3284 1870 Size: 73 73 68 531 669 4545 Fragments arranged by size: 4545 669 531 73 73 68 BamHI G'GATC_C Cuts at: 3028 3289 3656 3028 Size: 261 367 5331 Fragments arranged by size: 5331 367 261 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2238 2485 3280 4735 Size: 325 217 627 293 158 247 795 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 795 627 325 293 247 217 158 BanII G_rGCy'C Cuts at: 1391 2430 2721 3278 1391 Size: 1039 291 557 4072 Fragments arranged by size: 4072 1039 557 291 BbeI G_GCGC'C Cuts at: 2084 2242 2489 2084 Size: 158 247 5554 Fragments arranged by size: 5554 247 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2519 3100 928 Size: 971 620 581 3787 Fragments arranged by size: 3787 971 620 581 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2158 2192 2383 2417 3057 3091 4150 Size: 34 221 34 191 34 640 34 1059 Cuts at: 4150 4184 1903 Size: 34 3678 Fragments arranged by size: 3678 1059 640 221 191 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2483 4570 136 Size: 107 122 71 631 605 811 2087 1525 Fragments arranged by size: 2087 1525 811 631 605 122 107 71 BglII A'GATC_T Cuts at: 3021 5954 3021 Size: 2933 3026 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2818 4020 5176 1790 Size: 1028 1202 1156 2573 Fragments arranged by size: 2573 1202 1156 1028 BmgBI CAC'GTC Cuts at: 1318 2622 1318 Size: 1304 4655 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2686 2812 3690 Size: 416 53 83 399 24 1269 126 878 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 1269 958 878 416 399 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2109 4619 1239 Size: 870 2510 2579 Fragments arranged by size: 2579 2510 870 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2006 2895 4622 882 Size: 617 507 889 1727 2219 Fragments arranged by size: 2219 1727 889 617 507 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 2239 2486 3766 Size: 53 83 186 936 548 158 247 1280 Cuts at: 3766 4148 4783 275 Size: 382 635 1451 Fragments arranged by size: 1451 1280 936 635 548 382 247 186 158 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2107 2137 1178 Size: 30 165 30 704 30 5000 Fragments arranged by size: 5000 704 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2422 1186 Size: 1236 4723 BseYI C'CCAG_C Cuts at: 2584 5162 5658 2584 Size: 2578 496 2885 Fragments arranged by size: 2885 2578 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2029 2377 3135 1163 Size: 573 293 348 758 3987 Fragments arranged by size: 3987 758 573 348 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2331 2838 3409 4170 5198 1540 Size: 275 186 330 507 571 761 1028 2301 Fragments arranged by size: 2301 1028 761 571 507 330 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2562 2946 4020 4105 5176 2370 Size: 192 384 1074 85 1071 3153 Fragments arranged by size: 3153 1074 1071 384 192 85 BsmI GAATG_Cn' Cuts at: 2981 3470 3563 2981 Size: 489 93 5377 Fragments arranged by size: 5377 489 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2834 2834 Size: 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2370 2430 2562 2721 2818 2946 3278 Size: 399 580 60 132 159 97 128 332 Cuts at: 3278 4020 4105 5176 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 580 399 332 159 132 128 97 85 60 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 3314 844 Size: 2207 263 3489 Fragments arranged by size: 3489 2207 263 BsrBI CCG'CTC Cuts at: 1394 1823 2467 2896 3846 1394 Size: 429 644 429 950 3507 Fragments arranged by size: 3507 950 644 429 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 2578 4603 4790 1700 Size: 110 425 343 2025 187 2869 Fragments arranged by size: 2869 2025 425 343 187 110 BsrGI T'GTAC_A Cuts at: 96 3228 96 Size: 3132 2827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2219 3209 4019 5031 2219 Size: 990 810 1012 3147 Fragments arranged by size: 3147 1012 990 810 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 3067 4763 3067 Size: 1696 4263 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 2455 2455 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3021 3028 3057 3289 3656 4041 4058 5510 Size: 1049 7 29 232 367 385 17 1452 Cuts at: 5510 5596 5608 5954 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1049 385 367 346 232 86 29 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2157 2454 2796 513 Size: 552 1092 297 342 3676 Fragments arranged by size: 3676 1092 552 342 297 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2428 487 Size: 683 56 1202 4018 Fragments arranged by size: 4018 1202 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3475 4269 4289 2944 Size: 531 794 20 4614 Fragments arranged by size: 4614 794 531 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DraIII CAC_nnn'GTG Cuts at: 3158 3158 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2457 2475 Size: 54 1425 50 275 18 378 249 18 Cuts at: 2475 2553 2754 2844 3406 4295 5924 8 Size: 78 201 90 562 889 1629 43 Fragments arranged by size: 1629 1425 889 562 378 275 249 201 90 78 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2671 3889 1353 Size: 1318 1218 3423 Fragments arranged by size: 3423 1318 1218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2083 4546 4920 5066 387 Size: 1064 138 494 2463 374 146 1280 Fragments arranged by size: 2463 1280 1064 494 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 4022 4619 5406 5674 1239 Size: 393 477 1913 597 787 268 1524 Fragments arranged by size: 1913 1524 787 597 477 393 268 EcoO109I rG'GnC_Cy Cuts at: 2767 2767 Size: 5959 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2281 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2084 2242 2294 2454 2474 2489 2729 5106 Size: 406 158 52 160 20 15 240 2377 Cuts at: 5106 1678 Size: 2531 Fragments arranged by size: 2531 2377 406 240 160 158 52 20 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2911 2943 3013 Size: 32 135 32 560 32 747 32 70 Cuts at: 3013 3045 4600 4632 4674 4706 1373 Size: 32 1555 32 42 32 2626 Fragments arranged by size: 2626 1555 747 560 135 70 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2080 2238 2485 2080 Size: 158 247 5554 Fragments arranged by size: 5554 247 158 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 2516 5835 2516 Size: 3319 2640 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2477 2756 10 Size: 54 1425 343 378 267 279 3213 Fragments arranged by size: 3213 1425 378 343 279 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2339 2496 2870 3224 3917 4276 Size: 648 906 267 157 374 354 693 359 Cuts at: 4276 4435 518 Size: 159 2042 Fragments arranged by size: 2042 906 693 648 374 359 354 267 159 157 MspA1I CmG'CkG Cuts at: 1606 2781 3375 4052 5200 5445 1606 Size: 1175 594 677 1148 245 2120 Fragments arranged by size: 2120 1175 1148 677 594 245 NarI GG'CG_CC Cuts at: 2081 2239 2486 2081 Size: 158 247 5554 Fragments arranged by size: 5554 247 158 NcoI C'CATG_G Cuts at: 513 1065 2454 513 Size: 552 1389 4018 Fragments arranged by size: 4018 1389 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 2077 3317 4862 1132 Size: 97 848 1240 1545 2229 Fragments arranged by size: 2229 1545 1240 848 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2132 3025 1108 Size: 1024 893 4042 Fragments arranged by size: 4042 1024 893 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 3119 3311 830 Size: 2289 192 3478 Fragments arranged by size: 3478 2289 192 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2671 2671 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SexAI A'CCwGG_T Cuts at: 2301 2301 Size: 5959 SfcI C'TryA_G Cuts at: 826 1045 3115 3307 4442 5123 5314 826 Size: 219 2070 192 1135 681 191 1471 Fragments arranged by size: 2070 1471 1135 681 219 192 191 SfoI GGC'GCC Cuts at: 2082 2240 2487 2082 Size: 158 247 5554 Fragments arranged by size: 5554 247 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 2018 2617 3286 1872 Size: 146 599 669 4545 Fragments arranged by size: 4545 669 599 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2454 2790 3032 3398 513 Size: 552 1389 336 242 366 3074 Fragments arranged by size: 3074 1389 552 366 336 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2852 2869 3986 4003 4156 4341 4786 Size: 1255 1272 17 1117 17 153 185 445 Cuts at: 4786 325 Size: 1498 Fragments arranged by size: 1498 1272 1255 1117 445 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2598 3166 3228 Size: 274 80 33 51 1443 621 568 62 Cuts at: 3228 4205 5917 96 Size: 977 1712 138 Fragments arranged by size: 1712 1443 977 621 568 274 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2107 2139 5919 5951 2107 Size: 32 3780 32 2115 Fragments arranged by size: 3780 2115 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2303 1316 Size: 987 4972 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2767 2854 2767 Size: 87 5872 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 2016 2615 3284 1870 Size: 146 599 669 4545 Fragments arranged by size: 4545 669 599 146 XmnI GAAnn'nnTTC Cuts at: 2264 4088 2264 Size: 1824 4135 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI