(Linear) (Six-base) MAPSORT of: KIEE2195.seq Check: 4298 from: 1 to: 1254 KIEE2195.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AflIII A'CryG_T Cuts at: 0 511 1254 Size: 511 743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 509 1254 Size: 509 745 AlwNI CAG_nnn'CTG Cuts at: 0 127 1254 Size: 127 1127 AseI AT'TA_AT Cuts at: 0 1105 1217 1254 Size: 1105 112 37 Fragments arranged by size: 1105 112 37 AvaI C'yCGr_G Cuts at: 0 11 79 610 1254 Size: 11 68 531 644 Fragments arranged by size: 644 531 68 11 BamHI G'GATC_C Cuts at: 0 1023 1254 Size: 1023 231 BanI G'GyrC_C Cuts at: 0 75 233 480 1254 Size: 75 158 247 774 Fragments arranged by size: 774 247 158 75 BanII G_rGCy'C Cuts at: 0 425 716 1254 Size: 425 291 538 Fragments arranged by size: 538 425 291 BbeI G_GCGC'C Cuts at: 0 79 237 484 1254 Size: 79 158 247 770 Fragments arranged by size: 770 247 158 79 BbsI GAAGACnn'nnnn_ Cuts at: 0 514 1095 1254 Size: 514 581 159 Fragments arranged by size: 581 514 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 153 187 378 412 1052 1086 1254 Size: 153 34 191 34 640 34 168 Fragments arranged by size: 640 191 168 153 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 478 1254 Size: 478 776 BglII A'GATC_T Cuts at: 0 1016 1254 Size: 1016 238 Bme1580I G_kGCm'C Cuts at: 0 813 1254 Size: 813 441 BmgBI CAC'GTC Cuts at: 0 617 1254 Size: 617 637 BmrI ACTGGGnnnn_n' Cuts at: 0 681 807 1254 Size: 681 126 447 Fragments arranged by size: 681 447 126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 1254 Size: 104 1150 BsaI GGTCTCn'nnnn_ Cuts at: 0 1 890 1254 Size: 1 889 364 Fragments arranged by size: 889 364 1 BsaHI Gr'CG_yC Cuts at: 0 76 234 481 1254 Size: 76 158 247 773 Fragments arranged by size: 773 247 158 76 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 102 132 1254 Size: 102 30 1122 Fragments arranged by size: 1122 102 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 417 1254 Size: 417 837 BseYI C'CCAG_C Cuts at: 0 579 1254 Size: 579 675 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 372 1130 1254 Size: 24 348 758 124 Fragments arranged by size: 758 348 124 24 BsiEI CG_ry'CG Cuts at: 0 326 833 1254 Size: 326 507 421 Fragments arranged by size: 507 421 326 BsiHKAI G_wGCw'C Cuts at: 0 365 557 941 1254 Size: 365 192 384 313 Fragments arranged by size: 384 365 313 192 BsmI GAATG_Cn' Cuts at: 0 976 1254 Size: 976 278 BsmBI CGTCTCn'nnnn_ Cuts at: 0 829 1254 Size: 829 425 Bsp1286I G_dGCh'C Cuts at: 0 365 425 557 716 813 941 1254 Size: 365 60 132 159 97 128 313 Fragments arranged by size: 365 313 159 132 128 97 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1046 1254 Size: 1046 208 BsrBI CCG'CTC Cuts at: 0 462 891 1254 Size: 462 429 363 Fragments arranged by size: 462 429 363 BsrFI r'CCGG_y Cuts at: 0 230 573 1254 Size: 230 343 681 Fragments arranged by size: 681 343 230 BsrGI T'GTAC_A Cuts at: 0 1223 1254 Size: 1223 31 BssSI C'ACGA_G Cuts at: 0 214 1204 1254 Size: 214 990 50 Fragments arranged by size: 990 214 50 BstBI TT'CG_AA Cuts at: 0 1062 1254 Size: 1062 192 BstXI CCAn_nnnn'nTGG Cuts at: 0 450 1254 Size: 450 804 BstYI r'GATC_y Cuts at: 0 1016 1023 1052 1254 Size: 1016 7 29 202 Fragments arranged by size: 1016 202 29 7 BtgI C'CryG_G Cuts at: 0 152 449 791 1254 Size: 152 297 342 463 Fragments arranged by size: 463 342 297 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 423 1254 Size: 423 831 BtsI GCAGTG_nn' Cuts at: 0 939 1254 Size: 939 315 DraIII CAC_nnn'GTG Cuts at: 0 1153 1254 Size: 1153 101 EaeI y'GGCC_r Cuts at: 0 203 452 470 548 749 839 1254 Size: 203 249 18 78 201 90 415 Fragments arranged by size: 415 249 203 201 90 78 18 EarI CTCTTCn'nnn_ Cuts at: 0 666 1254 Size: 666 588 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 78 1254 Size: 78 1176 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 1254 Size: 104 1150 EcoO109I rG'GnC_Cy Cuts at: 0 762 1254 Size: 762 492 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 276 1254 Size: 276 978 HaeII r_GCGC'y Cuts at: 0 79 237 289 449 469 484 724 1254 Size: 79 158 52 160 20 15 240 530 Fragments arranged by size: 530 240 160 158 79 52 20 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 127 159 906 938 1008 1040 1254 Size: 127 32 747 32 70 32 214 Fragments arranged by size: 747 214 127 70 32 32 32 KasI G'GCGC_C Cuts at: 0 75 233 480 1254 Size: 75 158 247 774 Fragments arranged by size: 774 247 158 75 MluI A'CGCG_T Cuts at: 0 511 1254 Size: 511 743 MscI TGG'CCA Cuts at: 0 205 472 751 1254 Size: 205 267 279 503 Fragments arranged by size: 503 279 267 205 MslI CAynn'nnrTG Cuts at: 0 67 334 491 865 1219 1254 Size: 67 267 157 374 354 35 Fragments arranged by size: 374 354 267 157 67 35 MspA1I CmG'CkG Cuts at: 0 776 1254 Size: 776 478 NarI GG'CG_CC Cuts at: 0 76 234 481 1254 Size: 76 158 247 773 Fragments arranged by size: 773 247 158 76 NcoI C'CATG_G Cuts at: 0 449 1254 Size: 449 805 NspI r_CATG'y Cuts at: 0 72 1254 Size: 72 1182 PflMI CCAn_nnn'nTGG Cuts at: 0 127 1020 1254 Size: 127 893 234 Fragments arranged by size: 893 234 127 PstI C_TGCA'G Cuts at: 0 1114 1254 Size: 1114 140 SapI GCTCTTCn'nnn_ Cuts at: 0 666 1254 Size: 666 588 SexAI A'CCwGG_T Cuts at: 0 296 1254 Size: 296 958 SfcI C'TryA_G Cuts at: 0 1110 1254 Size: 1110 144 SfoI GGC'GCC Cuts at: 0 77 235 482 1254 Size: 77 158 247 772 Fragments arranged by size: 772 247 158 77 SmaI CCC'GGG Cuts at: 0 13 612 1254 Size: 13 599 642 Fragments arranged by size: 642 599 13 StyI C'CwwG_G Cuts at: 0 449 785 1027 1254 Size: 449 336 242 227 Fragments arranged by size: 449 336 242 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 847 864 1254 Size: 847 17 390 Fragments arranged by size: 847 390 17 TatI w'GTAC_w Cuts at: 0 593 1161 1223 1254 Size: 593 568 62 31 Fragments arranged by size: 593 568 62 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 102 134 1254 Size: 102 32 1120 Fragments arranged by size: 1120 102 32 Tth111I GACn'n_nGTC Cuts at: 0 298 1254 Size: 298 956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 762 849 1254 Size: 762 87 405 Fragments arranged by size: 762 405 87 XmaI C'CCGG_G Cuts at: 0 11 610 1254 Size: 11 599 644 Fragments arranged by size: 644 599 11 XmnI GAAnn'nnTTC Cuts at: 0 259 1254 Size: 259 995 Enzymes that do cut: AflIII AlfI AlwNI AseI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BglII Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I KasI MluI MscI MslI MspA1I NarI NcoI NspI PflMI PstI SapI SexAI SfcI SfoI SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BlpI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrDI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI ZraI