################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 480 ACATGT A^C[GA][CT]GT AlwNI 1759 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1477 CCTGAGG CC^T[ACGT]AGG BalI 93 TGGCCA TGG^CCA BanI 1031 GGCACC G^G[CT][GA]CC BfaI 604 CTAG C^TAG BpmI 1372 CTGGAG CTGGAG(16/14) BsgI 300 CTGCAC GTGCAG(16/14) BsmFI 314 GTCCC GGGAC(10/14) BspLU11I 480 ACATGT A^CATGT BspMI 1275 GCAGGT ACCTGC(4/8) BsrDI 1217 GCAATG GCAATG(2/0) BstXI 1715 CCATGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1160 GGATCT [GA]^GATC[CT] Bsu36I 1477 CCTGAGG CC^T[ACGT]AGG DraIII 1736 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 93 TGGCCA [CT]^GGCC[GA] Eco81I 1477 CCTGAGG CC^T[ACGT]AGG EcoRV 365 GATATC GAT^ATC FauI 1389 CCCGC CCCGC(4/6) HaeIII 94 GGCC GG^CC MaeI 604 CTAG C^TAG MfeI 740 CAATTG C^AATTG MflI 1160 GGATCT [GA]^GATC[CT] MluNI 93 TGGCCA TGG^CCA MscI 93 TGGCCA TGG^CCA MspA1I 54 CAGCTG C[AC]G^C[GT]G MunI 740 CAATTG C^AATTG NdeI 824 CATATG CA^TATG PstI 662 CTGCAG CTGCA^G PvuII 54 CAGCTG CAG^CTG SapI 505 GAAGAGC GCTCTTC(1/4) ScaI 572 AGTACT AGT^ACT SmlI 525 CTCAAG C^T[CT][GA]AG SnaBI 868 TACGTA TAC^GTA TaqI 1406 TCGA T^CGA TthHB8I 1406 TCGA T^CGA XcmI 1712 CCACCATGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1160 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 370 CCGC CCGC(-3/-1) 1390 CCGC AseI 238 ATTAAT AT^TAAT 1822 ATTAAT AsnI 238 ATTAAT AT^TAAT 1822 ATTAAT BmyI 703 GTGCCC G[AGT]GC[ACT]^C 1030 GGGCAC BsaAI 472 TACGTG [CT]AC^GT[GA] 868 TACGTA BsiYI 96 CCACATCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1787 CCTGTTTGGGG BslI 96 CCACATCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1787 CCTGTTTGGGG Bsp1286I 703 GTGCCC G[AGT]GC[ACT]^C 1030 GGGCAC DraII 1277 AGGTCCT [GA]G^G[ACGT]CC[CT] 1365 AGGACCC EcoO109I 1277 AGGTCCT [GA]G^G[ACGT]CC[CT] 1365 AGGACCC EcoT14I 181 CCAAGG C^C[AT][AT]GG 916 CCTTGG MaeII 473 ACGT A^CGT 869 ACGT MslI 335 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 637 CACCTGGATG NspI 285 ACATGC [GA]CATG^[CT] 480 ACATGT PleI 1549 GACTC GAGTC(4/5) 616 GAGTC PpuMI 1277 AGGTCCT [GA]G^G[AT]CC[CT] 1365 AGGACCC PshBI 238 ATTAAT AT^TAAT 1822 ATTAAT StyI 181 CCAAGG C^C[AT][AT]GG 916 CCTTGG Tsp45I 10 GTCAC ^GT[GC]AC 964 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbvI BsaJI BseRI BsmAI BsmI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TfiI Tru9I TseI Tsp509I TspRI XmnI