Restriction map for pFN21AE2035
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoT22I     FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflIII        480  ACATGT               A^C[GA][CT]GT
AlwNI        1759  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1477  CCTGAGG              CC^T[ACGT]AGG
BalI           93  TGGCCA               TGG^CCA
BanI         1031  GGCACC               G^G[CT][GA]CC
BfaI          604  CTAG                 C^TAG
BpmI         1372  CTGGAG               CTGGAG(16/14)
BsgI          300  CTGCAC               GTGCAG(16/14)
BsmFI         314  GTCCC                GGGAC(10/14)
BspLU11I      480  ACATGT               A^CATGT
BspMI        1275  GCAGGT               ACCTGC(4/8)
BsrDI        1217  GCAATG               GCAATG(2/0)
BstXI        1715  CCATGCTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1160  GGATCT               [GA]^GATC[CT]
Bsu36I       1477  CCTGAGG              CC^T[ACGT]AGG
DraIII       1736  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           93  TGGCCA               [CT]^GGCC[GA]
Eco81I       1477  CCTGAGG              CC^T[ACGT]AGG
EcoRV         365  GATATC               GAT^ATC
FauI         1389  CCCGC                CCCGC(4/6)
HaeIII         94  GGCC                 GG^CC
MaeI          604  CTAG                 C^TAG
MfeI          740  CAATTG               C^AATTG
MflI         1160  GGATCT               [GA]^GATC[CT]
MluNI          93  TGGCCA               TGG^CCA
MscI           93  TGGCCA               TGG^CCA
MspA1I         54  CAGCTG               C[AC]G^C[GT]G
MunI          740  CAATTG               C^AATTG
NdeI          824  CATATG               CA^TATG
PstI          662  CTGCAG               CTGCA^G
PvuII          54  CAGCTG               CAG^CTG
SapI          505  GAAGAGC              GCTCTTC(1/4)
ScaI          572  AGTACT               AGT^ACT
SmlI          525  CTCAAG               C^T[CT][GA]AG
SnaBI         868  TACGTA               TAC^GTA
TaqI         1406  TCGA                 T^CGA
TthHB8I      1406  TCGA                 T^CGA
XcmI         1712  CCACCATGCTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1160  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI          370  CCGC                 CCGC(-3/-1)
             1390  CCGC                
AseI          238  ATTAAT               AT^TAAT
             1822  ATTAAT              
AsnI          238  ATTAAT               AT^TAAT
             1822  ATTAAT              
BmyI          703  GTGCCC               G[AGT]GC[ACT]^C
             1030  GGGCAC              
BsaAI         472  TACGTG               [CT]AC^GT[GA]
              868  TACGTA              
BsiYI          96  CCACATCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1787  CCTGTTTGGGG         
BslI           96  CCACATCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1787  CCTGTTTGGGG         
Bsp1286I      703  GTGCCC               G[AGT]GC[ACT]^C
             1030  GGGCAC              
DraII        1277  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1365  AGGACCC             
EcoO109I     1277  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1365  AGGACCC             
EcoT14I       181  CCAAGG               C^C[AT][AT]GG
              916  CCTTGG              
MaeII         473  ACGT                 A^CGT
              869  ACGT                
MslI          335  CACTGATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              637  CACCTGGATG          
NspI          285  ACATGC               [GA]CATG^[CT]
              480  ACATGT              
PleI         1549  GACTC                GAGTC(4/5)
              616  GAGTC               
PpuMI        1277  AGGTCCT              [GA]G^G[AT]CC[CT]
             1365  AGGACCC             
PshBI         238  ATTAAT               AT^TAAT
             1822  ATTAAT              
StyI          181  CCAAGG               C^C[AT][AT]GG
              916  CCTTGG              
Tsp45I         10  GTCAC                ^GT[GC]AC
              964  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AvaII       BbvI        BsaJI       BseRI       
BsmAI       BsmI        BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EarI        
EcoRI       EcoRII      Fnu4HI      FokI        HinfI       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XmnI        
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