(Circular) (Six-base) MAPSORT of: pFN21AE3761.seq Check: 3696 from: 1 to: 6361 pFN21AE3761 6361 bp With 182 enzymes: SgfI * March 28, 2012 12:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4171 278 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3704 1937 Size: 1767 4594 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 4488 4861 1563 Size: 2925 373 3063 Fragments arranged by size: 3063 2925 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2571 2604 2859 3276 3567 4424 5808 6076 Size: 939 33 255 417 291 857 1384 268 Cuts at: 6076 1632 Size: 1917 Fragments arranged by size: 1917 1384 939 857 417 291 268 255 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6164 AflIII A'CryG_T Cuts at: 1203 5260 6237 1203 Size: 4057 977 1327 Fragments arranged by size: 4057 1327 977 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 5090 5090 Size: 6361 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6361 AlwNI CAG_nnn'CTG Cuts at: 2374 2549 2582 3344 4104 5676 2374 Size: 175 33 762 760 1572 3059 Fragments arranged by size: 3059 1572 762 760 175 33 ApaLI G'TGCA_C Cuts at: 4418 5574 4418 Size: 1156 5205 ApoI r'AATT_y Cuts at: 1493 2196 2507 2900 3670 3896 4048 6157 1493 Size: 703 311 393 770 226 152 2109 1697 Fragments arranged by size: 2109 1697 770 703 393 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 3686 1870 Size: 73 1743 4545 Fragments arranged by size: 4545 1743 73 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2633 3682 5137 618 Size: 325 217 627 291 555 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 627 555 325 291 217 BanII G_rGCy'C Cuts at: 1391 3680 1391 Size: 2289 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2847 3573 928 Size: 971 948 726 3716 Fragments arranged by size: 3716 971 948 726 BbvCI CC'TCA_GC Cuts at: 3462 3462 Size: 6361 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4552 4586 1903 Size: 34 2615 34 3678 Fragments arranged by size: 3678 2615 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4253 5463 4253 Size: 1210 5151 BclI T'GATC_A Cuts at: 1548 2250 1548 Size: 702 5659 BfrBI ATG'CAT Cuts at: 2743 3076 3308 2743 Size: 333 232 5796 Fragments arranged by size: 5796 333 232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4972 136 Size: 107 122 71 631 605 3300 1525 Fragments arranged by size: 3300 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3443 6356 3443 Size: 2913 3448 BlpI GC'TnA_GC Cuts at: 3778 3778 Size: 6361 Bme1580I G_kGCm'C Cuts at: 1790 4422 5578 1790 Size: 2632 1156 2573 Fragments arranged by size: 2632 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2817 4092 5050 Size: 416 53 83 399 24 1400 1275 958 Cuts at: 5050 442 Size: 1753 Fragments arranged by size: 1753 1400 1275 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2316 2617 5021 1239 Size: 1077 301 2404 2579 Fragments arranged by size: 2579 2404 1077 301 Bpu10I CC'TnA_GC Cuts at: 3462 3462 Size: 6361 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2937 4486 5351 5649 2937 Size: 1549 865 298 3649 Fragments arranged by size: 3649 1549 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2412 5024 882 Size: 617 913 2612 2219 Fragments arranged by size: 2612 2219 913 617 BsaAI yAC'GTr Cuts at: 493 1204 2053 3355 4152 493 Size: 711 849 1302 797 2702 Fragments arranged by size: 2702 1302 849 797 711 BsaBI GATnn'nnATC Cuts at: 6355 6355 Size: 6361 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 4168 4550 5185 Size: 53 83 186 936 540 2095 382 635 Cuts at: 5185 275 Size: 1451 Fragments arranged by size: 2095 1451 936 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3566 4794 5192 5466 5613 6024 3566 Size: 1228 398 274 147 411 3903 Fragments arranged by size: 3903 1228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2590 2620 1178 Size: 30 165 30 1187 30 4919 Fragments arranged by size: 4919 1187 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6361 BseYI C'CCAG_C Cuts at: 2056 2068 5564 6060 2056 Size: 12 3496 496 2357 Fragments arranged by size: 3496 2357 496 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5788 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3811 4572 5600 1540 Size: 275 186 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4422 4507 5578 4422 Size: 85 1071 5205 Fragments arranged by size: 5205 1071 85 BsmI GAATG_Cn' Cuts at: 2464 3310 3872 3965 2464 Size: 846 562 93 4860 Fragments arranged by size: 4860 846 562 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3680 4422 4507 5578 1391 Size: 399 1890 742 85 1071 2174 Fragments arranged by size: 2174 1890 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6024 6024 Size: 6361 BspHI T'CATG_A Cuts at: 4250 5980 4250 Size: 1730 4631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2567 2743 3716 844 Size: 1723 176 973 3489 Fragments arranged by size: 3489 1723 973 176 BsrBI CCG'CTC Cuts at: 1394 1823 4248 1394 Size: 429 2425 3507 Fragments arranged by size: 3507 2425 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4856 5030 65 Size: 1183 3608 174 1396 Fragments arranged by size: 3608 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5005 5192 1700 Size: 110 3195 187 2869 Fragments arranged by size: 3195 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2909 96 Size: 2813 3548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6361 BssSI C'ACGA_G Cuts at: 4421 5433 4421 Size: 1012 5349 BstAPI GCAn_nnn'nTGC Cuts at: 794 2749 794 Size: 1955 4406 BstBI TT'CG_AA Cuts at: 5165 5165 Size: 6361 BstEII G'GTnAC_C Cuts at: 1184 2945 1184 Size: 1761 4600 BstYI r'GATC_y Cuts at: 1972 2528 2679 3443 3691 4058 4443 4460 5912 Size: 556 151 764 248 367 385 17 1452 Cuts at: 5912 5998 6010 6356 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 764 556 385 367 346 248 151 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3612 513 Size: 552 939 1608 3262 Fragments arranged by size: 3262 1608 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5622 Fragments arranged by size: 5622 683 56 BtsI GCAGTG_nn' Cuts at: 2973 3877 4671 4691 2973 Size: 904 794 20 4643 Fragments arranged by size: 4643 904 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6326 DraI TTT'AAA Cuts at: 3412 3665 3412 Size: 253 6108 DraIII CAC_nnn'GTG Cuts at: 2053 2053 Size: 6361 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5368 809 Size: 507 4052 1802 Fragments arranged by size: 4052 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3808 4697 6326 Size: 54 1425 50 275 18 1978 889 1629 Cuts at: 6326 8 Size: 43 Fragments arranged by size: 1978 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3808 1537 Size: 275 1996 4090 Fragments arranged by size: 4090 1996 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 2628 4291 1353 Size: 981 294 1663 3423 Fragments arranged by size: 3423 1663 981 294 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4948 5322 5468 387 Size: 1064 138 3359 374 146 1280 Fragments arranged by size: 3359 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 2571 2604 2617 2859 3276 3567 Size: 393 684 255 33 13 242 417 291 Cuts at: 3567 4424 5021 5808 6076 1239 Size: 857 597 787 268 1524 Fragments arranged by size: 1524 857 787 684 597 417 393 291 268 255 242 33 13 EcoO109I rG'GnC_Cy Cuts at: 2579 2579 Size: 6361 EcoRI G'AATT_C Cuts at: 3670 4048 3670 Size: 378 5983 EcoRV GAT'ATC Cuts at: 2593 2593 Size: 6361 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2978 2978 Size: 6361 FspI TGC'GCA Cuts at: 4867 4867 Size: 6361 HaeII r_GCGC'y Cuts at: 1678 5508 1678 Size: 3830 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2242 2274 2676 2708 2763 Size: 32 135 32 670 32 402 32 55 Cuts at: 2763 2795 3103 3135 3428 3460 5002 5034 5076 Size: 32 308 32 293 32 1542 32 42 Cuts at: 5076 5108 1373 Size: 32 2626 Fragments arranged by size: 2626 1542 670 402 308 293 135 55 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2758 3705 3951 669 Size: 988 281 820 947 246 3079 Fragments arranged by size: 3079 988 947 820 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6361 HpaI GTT'AAC Cuts at: 3951 3951 Size: 6361 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 3960 3960 Size: 6361 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2158 2285 2424 5475 5659 1305 Size: 853 127 139 3051 184 2007 Fragments arranged by size: 3051 2007 853 184 139 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4539 Fragments arranged by size: 4539 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2457 3318 4319 4678 4837 518 Size: 648 1291 861 1001 359 159 2042 Fragments arranged by size: 2042 1291 1001 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3280 3777 4454 5602 5847 1606 Size: 1674 497 677 1148 245 2120 Fragments arranged by size: 2120 1674 1148 677 497 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3612 513 Size: 552 939 1608 3262 Fragments arranged by size: 3262 1608 939 552 NdeI CA'TA_TG Cuts at: 387 2403 2745 387 Size: 2016 342 4003 Fragments arranged by size: 4003 2016 342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NsiI A_TGCA'T Cuts at: 2745 3078 3310 2745 Size: 333 232 5796 Fragments arranged by size: 5796 333 232 NspI r_CATG'y Cuts at: 1132 1229 2660 3719 5264 1132 Size: 97 1431 1059 1545 2229 Fragments arranged by size: 2229 1545 1431 1059 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6361 PciI A'CATG_T Cuts at: 5260 5260 Size: 6361 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 3344 1108 Size: 1020 1216 4125 Fragments arranged by size: 4125 1216 1020 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3433 3465 4430 4462 5943 5975 3433 Size: 32 965 32 1481 32 3819 Fragments arranged by size: 3819 1481 965 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2579 2579 Size: 6361 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6361 PsiI TTA'TAA Cuts at: 3931 3931 Size: 6361 PstI C_TGCA'G Cuts at: 830 2754 3526 3713 830 Size: 1924 772 187 3478 Fragments arranged by size: 3478 1924 772 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 PvuII CAG'CTG Cuts at: 3280 3280 Size: 6361 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SapI GCTCTTCn'nnn_ Cuts at: 2628 2628 Size: 6361 SbfI CC_TGCA'GG Cuts at: 3713 3713 Size: 6361 ScaI AGT'ACT Cuts at: 4609 6321 4609 Size: 1712 4649 SexAI A'CCwGG_T Cuts at: 2244 3378 2244 Size: 1134 5227 SfcI C'TryA_G Cuts at: 826 1045 2750 3522 3709 4844 5525 5716 826 Size: 219 1705 772 187 1135 681 191 1471 Fragments arranged by size: 1705 1471 1135 772 681 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 3688 1872 Size: 1816 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2952 4465 5366 5628 820 Size: 197 926 1009 1513 901 262 1553 Fragments arranged by size: 1553 1513 1009 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4152 493 Size: 3659 2702 SpeI A'CTAG_T Cuts at: 152 2519 152 Size: 2367 3994 SphI G_CATG'C Cuts at: 1132 3719 1132 Size: 2587 3774 SspI AAT'ATT Cuts at: 5 52 4285 5 Size: 47 4233 2081 Fragments arranged by size: 4233 2081 47 StuI AGG'CCT Cuts at: 1517 3090 1517 Size: 1573 4788 StyI C'CwwG_G Cuts at: 513 1065 2004 3612 3800 513 Size: 552 939 1608 188 3074 Fragments arranged by size: 3074 1608 939 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4388 4405 4558 4743 5188 325 Size: 1255 2808 17 153 185 445 1498 Fragments arranged by size: 2808 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2909 4607 6319 Size: 274 80 33 51 1443 932 1698 1712 Cuts at: 6319 96 Size: 138 Fragments arranged by size: 1712 1698 1443 932 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2037 2069 2590 2622 6321 6353 2037 Size: 32 521 32 3699 32 2045 Fragments arranged by size: 3699 2045 521 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6361 XbaI T'CTAG_A Cuts at: 3697 3697 Size: 6361 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3539 3539 Size: 6361 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6361 XmaI C'CCGG_G Cuts at: 1870 3686 1870 Size: 1816 4545 XmnI GAAnn'nnTTC Cuts at: 4490 4490 Size: 6361 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4169 276 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI