Restriction map for
pFN21AE1179
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRV EcoT22I FbaI FspI
HindIII HpaI Ksp632I MfeI MluI
MroI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1694 GGTACC G^GTACC
AccI 1949 GTCGAC GT^[AC][GT]AC
AcsI 1244 GAATTC [GA]^AATT[CT]
ApoI 1244 GAATTC [GA]^AATT[CT]
Asp718I 1694 GGTACC G^GTACC
AspHI 55 GTGCTC G[AT]GC[AT]^C
AvrII 805 CCTAGG C^CTAGG
BbeI 1450 GGCGCC GGCGC^C
BbsI 1299 GAAGAC GAAGAC(2/6)
BfaI 806 CTAG C^TAG
BlnI 805 CCTAGG C^CTAGG
BpuAI 1299 GAAGAC GAAGAC(2/6)
BsaAI 1409 TACGTG [CT]AC^GT[GA]
BsaI 1046 GAGACC GGTCTC(1/5)
BsgI 959 GTGCAG GTGCAG(16/14)
BsiHKAI 55 GTGCTC G[AT]GC[AT]^C
BsmBI 733 GAGACG CGTCTC(1/5)
BsrBI 91 GAGCGG CCGCTC(-3/-3)
DrdI 677 GACCACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1244 GAATTC G^AATTC
HgaI 1271 GCGTC GACGC(5/10)
HincII 1949 GTCGAC GT[CT]^[GA]AC
HindII 1949 GTCGAC GT[CT]^[GA]AC
KasI 1450 GGCGCC G^GCGCC
KpnI 1694 GGTACC GGTAC^C
KspI 1311 CCGCGG CCGC^GG
MaeI 806 CTAG C^TAG
MseI 253 TTAA T^TAA
NarI 1450 GGCGCC GG^CGCC
NspI 579 GCATGC [GA]CATG^[CT]
PflMI 489 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 562 CTGCAG CTGCA^G
SacII 1311 CCGCGG CCGC^GG
SalI 1949 GTCGAC G^TCGAC
SexAI 468 ACCTGGT A^CC[AT]GGT
SfcI 562 CTGCAG C^T[GA][CT]AG
SfoI 1450 GGCGCC GGC^GCC
SphI 579 GCATGC GCATG^C
Tru9I 253 TTAA T^TAA
Van91I 489 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 592 CGCG CG^CG
1312 CGCG
AcyI 1270 GGCGTC G[GA]^CG[CT]C
1450 GGCGCC
AfaI 1408 GTAC GT^AC
1695 GTAC
AlwNI 490 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2042 CAGAGCCTG
AocI 1201 CCTCAGG CC^T[ACGT]AGG
1219 CCTAAGG
BalI 612 TGGCCA TGG^CCA
1887 TGGCCA
BsaBI 396 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1909 GATGGAGATC
BsaHI 1270 GGCGTC G[GA]^CG[CT]C
1450 GGCGCC
BseRI 40 CTCCTC GAGGAG(10/8)
1124 GAGGAG
BspMI 1995 ACCTGC ACCTGC(4/8)
1945 GCAGGT
BsrFI 1339 GCCGGC [GA]^CCGG[CT]
1880 GCCGGC
BstEII 1162 GGTGACC G^GT[ACGT]ACC
1324 GGTGACC
BstPI 1162 GGTGACC G^GT[ACGT]ACC
1324 GGTGACC
BstUI 592 CGCG CG^CG
1312 CGCG
Bsu36I 1201 CCTCAGG CC^T[ACGT]AGG
1219 CCTAAGG
Cfr10I 1339 GCCGGC [GA]^CCGG[CT]
1880 GCCGGC
DdeI 1202 CTCAG C^T[ACGT]AG
1220 CTAAG
DraIII 788 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1570 CACATCGTG
Eco81I 1201 CCTCAGG CC^T[ACGT]AGG
1219 CCTAAGG
EcoO65I 1162 GGTGACC G^GT[ACGT]ACC
1324 GGTGACC
FseI 1338 GGCCGGCC GGCCGG^CC
1879 GGCCGGCC
Hin1I 1270 GGCGTC G[GA]^CG[CT]C
1450 GGCGCC
MaeII 1410 ACGT A^CGT
1797 ACGT
MamI 396 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1909 GATGGAGATC
MboII 1299 GAAGA GAAGA(8/7)
1590 GAAGA
MluNI 612 TGGCCA TGG^CCA
1887 TGGCCA
MscI 612 TGGCCA TGG^CCA
1887 TGGCCA
MspA1I 1311 CCGCGG C[AC]G^C[GT]G
1437 CAGCGG
MvnI 592 CGCG CG^CG
1312 CGCG
NaeI 1339 GCCGGC GCC^GGC
1880 GCCGGC
NcoI 281 CCATGG C^CATGG
1716 CCATGG
NgoMIV 1339 GCCGGC G^CCGGC
1880 GCCGGC
PaeR7I 1121 CTCGAG C^TCGAG
1490 CTCGAG
RsaI 1408 GTAC GT^AC
1695 GTAC
SmlI 1121 CTCGAG C^T[CT][GA]AG
1490 CTCGAG
Tsp509I 1131 AATT ^AATT
1245 AATT
XhoI 1121 CTCGAG C^TCGAG
1490 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
AvaII BamHI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
SfiI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI