(Circular) (Six-base) MAPSORT of: pFN21AE1179.seq Check: 7805 from: 1 to: 6772 pFN21AE1179 6772 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4582 278 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3955 4115 1937 Size: 2018 160 4594 Fragments arranged by size: 4594 2018 160 Acc65I G'GTAC_C Cuts at: 3699 4093 3699 Size: 394 6378 AclI AA'CG_TT Cuts at: 1563 4899 5272 1563 Size: 3336 373 3063 Fragments arranged by size: 3336 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3614 4835 6219 6487 1632 Size: 1982 1221 1384 268 1917 Fragments arranged by size: 1982 1917 1384 1221 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6575 AflIII A'CryG_T Cuts at: 1203 5671 6648 1203 Size: 4468 977 1327 Fragments arranged by size: 4468 1327 977 AgeI A'CCGG_T Cuts at: 5603 5603 Size: 6772 AhdI GACnn_n'nnGTC Cuts at: 5501 5501 Size: 6772 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6772 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6772 AlwNI CAG_nnn'CTG Cuts at: 2500 4052 4515 6087 2500 Size: 1552 463 1572 3185 Fragments arranged by size: 3185 1572 1552 463 ApaI G_GGCC'C Cuts at: 2105 2169 2348 2454 2730 2747 2909 3071 3224 Size: 64 179 106 276 17 162 162 153 Cuts at: 3224 3242 3261 3315 3396 3693 3719 3792 3861 Size: 18 19 54 81 297 26 73 69 Cuts at: 3861 2105 Size: 5016 Fragments arranged by size: 5016 297 276 179 162 162 153 106 81 73 69 64 54 26 19 18 17 ApaLI G'TGCA_C Cuts at: 4829 5985 4829 Size: 1156 5616 ApoI r'AATT_y Cuts at: 1493 3249 4081 4307 4459 6568 1493 Size: 1756 832 226 152 2109 1697 Fragments arranged by size: 2109 1756 1697 832 226 152 AseI AT'TA_AT Cuts at: 160 1032 5326 160 Size: 872 4294 1606 Fragments arranged by size: 4294 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2116 2243 2315 2482 3126 3495 Size: 73 164 9 127 72 167 644 369 Cuts at: 3495 3533 3738 3845 4097 1870 Size: 38 205 107 252 4545 Fragments arranged by size: 4545 644 369 252 205 167 164 127 107 73 72 38 9 AvrII C'CTAG_G Cuts at: 2810 2810 Size: 6772 BamHI G'GATC_C Cuts at: 2138 2292 3867 4055 4102 4469 2138 Size: 154 1575 188 47 367 4441 Fragments arranged by size: 4441 1575 367 188 154 47 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2654 2780 3455 3699 3726 Size: 325 217 627 867 126 675 244 27 Cuts at: 3726 3840 4093 5548 618 Size: 114 253 1455 1842 Fragments arranged by size: 1842 1455 867 675 627 325 253 244 217 126 114 27 BanII G_rGCy'C Cuts at: 1391 2105 2169 2348 2454 2730 2747 2909 3071 Size: 714 64 179 106 276 17 162 162 Cuts at: 3071 3224 3242 3261 3296 3315 3396 3693 3719 Size: 153 18 19 35 19 81 297 26 Cuts at: 3719 3792 3861 3940 4091 1391 Size: 73 69 79 151 4072 Fragments arranged by size: 4072 714 297 276 179 162 162 153 151 106 81 79 73 69 64 35 26 19 19 18 17 BbeI G_GCGC'C Cuts at: 3459 3459 Size: 6772 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3311 928 Size: 971 1412 4389 Fragments arranged by size: 4389 1412 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4963 4997 1903 Size: 34 3026 34 3678 Fragments arranged by size: 3678 3026 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4664 5874 4664 Size: 1210 5562 BclI T'GATC_A Cuts at: 1548 1548 Size: 6772 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 2227 2343 Size: 107 122 71 631 605 346 209 116 Cuts at: 2343 2913 3003 3075 3741 3891 3969 5383 136 Size: 570 90 72 666 150 78 1414 1525 Fragments arranged by size: 1525 1414 666 631 605 570 346 209 150 122 116 107 90 78 72 71 BglII A'GATC_T Cuts at: 6767 6767 Size: 6772 BlpI GC'TnA_GC Cuts at: 4189 4189 Size: 6772 Bme1580I G_kGCm'C Cuts at: 1790 2105 2169 2348 2454 2730 2747 2909 3071 Size: 315 64 179 106 276 17 162 162 Cuts at: 3071 3224 3242 3261 3315 3396 3693 3719 3729 Size: 153 18 19 54 81 297 26 10 Cuts at: 3729 3792 3843 3861 4833 5989 1790 Size: 63 51 18 972 1156 2573 Fragments arranged by size: 2573 1156 972 315 297 276 179 162 162 153 106 81 64 63 54 51 26 19 18 18 17 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6772 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2796 4503 5461 Size: 416 53 83 399 24 1379 1707 958 Cuts at: 5461 442 Size: 1753 Fragments arranged by size: 1753 1707 1379 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6772 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2283 2319 2682 3069 3660 3924 5432 Size: 794 250 36 363 387 591 264 1508 Cuts at: 5432 1239 Size: 2579 Fragments arranged by size: 2579 1508 794 591 387 363 264 250 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4897 5762 6060 4897 Size: 865 298 5609 Fragments arranged by size: 5609 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3045 5435 882 Size: 617 1546 2390 2219 Fragments arranged by size: 2390 2219 1546 617 BsaAI yAC'GTr Cuts at: 493 1204 3416 4563 493 Size: 711 2212 1147 2702 Fragments arranged by size: 2702 2212 1147 711 BsaBI GATnn'nnATC Cuts at: 2405 3918 6766 2405 Size: 1513 2848 2411 Fragments arranged by size: 2848 2411 1513 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3276 3456 4579 4961 Size: 53 83 186 936 1743 180 1123 382 Cuts at: 4961 5596 275 Size: 635 1451 Fragments arranged by size: 1743 1451 1123 936 635 382 186 180 83 53 BsaWI w'CCGG_w Cuts at: 5205 5603 5877 6024 6435 5205 Size: 398 274 147 411 5542 Fragments arranged by size: 5542 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3278 3308 1178 Size: 30 165 30 1875 30 4642 Fragments arranged by size: 4642 1875 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 3144 1186 Size: 850 1108 4814 Fragments arranged by size: 4814 1108 850 BseYI C'CCAG_C Cuts at: 2216 2831 3440 5975 6471 2216 Size: 615 609 2535 496 2517 Fragments arranged by size: 2535 2517 615 609 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2985 1163 Size: 573 1249 4950 Fragments arranged by size: 4950 1249 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4222 4983 6011 1540 Size: 275 186 2221 761 1028 2301 Fragments arranged by size: 2301 2221 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 4833 4918 5989 2064 Size: 2769 85 1071 2847 Fragments arranged by size: 2847 2769 1071 85 BsmI GAATG_Cn' Cuts at: 4283 4376 4283 Size: 93 6679 BsmBI CGTCTCn'nnnn_ Cuts at: 2732 2732 Size: 6772 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2105 2169 2348 2454 2730 2747 Size: 399 274 41 64 179 106 276 17 Cuts at: 2747 2909 3071 3224 3242 3261 3296 3315 3396 Size: 162 162 153 18 19 35 19 81 Cuts at: 3396 3693 3719 3729 3792 3843 3861 3940 4091 Size: 297 26 10 63 51 18 79 151 Cuts at: 4091 4833 4918 5989 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 297 276 274 179 162 162 153 151 106 85 81 79 64 63 51 41 35 26 19 19 18 18 17 10 BspEI T'CCGG_A Cuts at: 6435 6435 Size: 6772 BspHI T'CATG_A Cuts at: 4661 6391 4661 Size: 1730 5042 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3941 4009 4127 844 Size: 3097 68 118 3489 Fragments arranged by size: 3489 3097 118 68 BsrBI CCG'CTC Cuts at: 1394 1823 2098 4659 1394 Size: 429 275 2561 3507 Fragments arranged by size: 3507 2561 429 275 BsrDI GCAATG_nn' Cuts at: 65 1248 5267 5441 65 Size: 1183 4019 174 1396 Fragments arranged by size: 4019 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3344 3885 5416 5603 1700 Size: 110 1534 541 1531 187 2869 Fragments arranged by size: 2869 1534 1531 541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6772 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6772 BssSI C'ACGA_G Cuts at: 4832 5844 4832 Size: 1012 5760 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6772 BstBI TT'CG_AA Cuts at: 5576 5576 Size: 6772 BstEII G'GTnAC_C Cuts at: 1184 3167 3329 1184 Size: 1983 162 4627 Fragments arranged by size: 4627 1983 162 BstYI r'GATC_y Cuts at: 1972 2138 2292 3867 4055 4102 4469 4854 4871 Size: 166 154 1575 188 47 367 385 17 Cuts at: 4871 6323 6409 6421 6767 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1575 1452 385 367 346 188 166 154 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 3207 3225 3207 Size: 18 6754 BtgI C'CryG_G Cuts at: 513 1065 2004 2286 3316 3721 3765 3975 513 Size: 552 939 282 1030 405 44 210 3310 Fragments arranged by size: 3310 1030 939 552 405 282 210 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6033 Fragments arranged by size: 6033 683 56 BtsI GCAGTG_nn' Cuts at: 2515 2968 4288 5082 5102 2515 Size: 453 1320 794 20 4185 Fragments arranged by size: 4185 1320 794 453 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6772 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6737 DraI TTT'AAA Cuts at: 4076 4076 Size: 6772 DraIII CAC_nnn'GTG Cuts at: 2798 3580 2798 Size: 782 5990 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2688 5779 809 Size: 507 1372 3091 1802 Fragments arranged by size: 3091 1802 1372 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2617 3342 Size: 54 1425 50 275 18 177 610 725 Cuts at: 3342 3504 3883 3892 4219 5108 6737 8 Size: 162 379 9 327 889 1629 43 Fragments arranged by size: 1629 1425 889 725 610 379 327 275 177 162 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 4219 1537 Size: 275 2407 4090 Fragments arranged by size: 4090 2407 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4702 1353 Size: 3349 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5359 5733 5879 387 Size: 1064 138 3770 374 146 1280 Fragments arranged by size: 3770 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2283 2319 2682 3069 3614 3660 Size: 393 401 250 36 363 387 545 46 Cuts at: 3660 3924 4835 5432 6219 6487 1239 Size: 264 911 597 787 268 1524 Fragments arranged by size: 1524 911 787 597 545 401 393 387 363 268 264 250 46 36 EcoO109I rG'GnC_Cy Cuts at: 2101 2102 2111 2166 2192 2345 2414 2425 2450 Size: 1 9 55 26 153 69 11 25 Cuts at: 2450 2451 2459 2555 2636 2690 2726 2744 2807 Size: 1 8 96 81 54 36 18 63 Cuts at: 2807 2906 2934 3044 3076 3122 3203 3220 3221 Size: 99 28 110 32 46 81 17 1 Cuts at: 3221 3238 3239 3257 3258 3312 3392 3671 3680 Size: 17 1 18 1 54 80 279 9 Cuts at: 3680 3690 3715 3716 3788 3858 3927 3971 4067 Size: 10 25 1 72 70 69 44 96 Cuts at: 4067 2101 Size: 4806 Fragments arranged by size: 4806 279 153 110 99 96 96 81 81 80 72 70 69 69 63 55 54 54 46 44 36 32 28 26 25 25 18 18 17 17 11 10 9 9 8 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 3249 4081 4459 3249 Size: 832 378 5562 Fragments arranged by size: 5562 832 378 FseI GG_CCGG'CC Cuts at: 3348 3889 3348 Size: 541 6231 FspI TGC'GCA Cuts at: 5278 5278 Size: 6772 HaeII r_GCGC'y Cuts at: 1678 2775 3459 3593 5919 1678 Size: 1097 684 134 2326 2531 Fragments arranged by size: 2531 2326 1097 684 134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2068 2100 2429 2461 2731 Size: 32 135 32 496 32 329 32 270 Cuts at: 2731 2763 3278 3310 3658 3690 3911 3943 4040 Size: 32 515 32 348 32 221 32 97 Cuts at: 4040 4072 5413 5445 5487 5519 1373 Size: 32 1341 32 42 32 2626 Fragments arranged by size: 2626 1341 515 496 348 329 270 221 135 97 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3956 4116 4362 669 Size: 988 281 2018 160 246 3079 Fragments arranged by size: 3079 2018 988 281 246 160 HindIII A'AGCT_T Cuts at: 748 748 Size: 6772 HpaI GTT'AAC Cuts at: 4362 4362 Size: 6772 KasI G'GCGC_C Cuts at: 3455 3455 Size: 6772 KpnI G_GTAC'C Cuts at: 3703 4097 3703 Size: 394 6378 MfeI C'AATT_G Cuts at: 4371 4371 Size: 6772 MluI A'CGCG_T Cuts at: 6648 6648 Size: 6772 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 2552 5886 6070 1305 Size: 861 386 3334 184 2007 Fragments arranged by size: 3334 2007 861 386 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2619 3894 10 Size: 54 1425 343 787 1275 2888 Fragments arranged by size: 2888 1425 1275 787 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4730 5089 5248 518 Size: 648 3564 359 159 2042 Fragments arranged by size: 3564 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3318 3444 4188 4865 6013 6258 1606 Size: 1712 126 744 677 1148 245 2120 Fragments arranged by size: 2120 1712 1148 744 677 245 126 NaeI GCC'GGC Cuts at: 3346 3887 3346 Size: 541 6231 NarI GG'CG_CC Cuts at: 3456 3456 Size: 6772 NcoI C'CATG_G Cuts at: 513 1065 2004 2286 3721 513 Size: 552 939 282 1435 3564 Fragments arranged by size: 3564 1435 939 552 282 NdeI CA'TA_TG Cuts at: 387 387 Size: 6772 NgoMIV G'CCGG_C Cuts at: 3344 3885 3344 Size: 541 6231 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6772 NotI GC'GGCC_GC Cuts at: 4219 4219 Size: 6772 NspI r_CATG'y Cuts at: 1132 1229 2588 4130 5675 1132 Size: 97 1359 1542 1545 2229 Fragments arranged by size: 2229 1545 1542 1359 97 PasI CC'CwG_GG Cuts at: 1348 2465 2561 2695 2875 3101 3145 3299 3335 Size: 1117 96 134 180 226 44 154 36 Cuts at: 3335 3461 3649 3695 3863 3932 1348 Size: 126 188 46 168 69 4188 Fragments arranged by size: 4188 1117 226 188 180 168 154 134 126 96 69 46 44 36 PciI A'CATG_T Cuts at: 5671 5671 Size: 6772 PflMI CCAn_nnn'nTGG Cuts at: 1108 2500 1108 Size: 1392 5380 PfoI T'CCnGG_A Cuts at: 2188 2374 3082 3676 3820 3940 2188 Size: 186 708 594 144 120 5020 Fragments arranged by size: 5020 708 594 186 144 120 PmeI GTTT'AAAC Cuts at: 4076 4076 Size: 6772 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4841 4873 6354 6386 4841 Size: 32 1481 32 5227 Fragments arranged by size: 5227 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2111 2192 2425 2459 2555 2636 2690 2807 2934 Size: 81 233 34 96 81 54 117 127 Cuts at: 2934 3671 3680 3927 2111 Size: 737 9 247 4956 Fragments arranged by size: 4956 737 247 233 127 117 96 81 81 54 34 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6772 PsiI TTA'TAA Cuts at: 4342 4342 Size: 6772 PspOMI G'GGCC_C Cuts at: 2101 2165 2344 2450 2726 2743 2905 3067 3220 Size: 64 179 106 276 17 162 162 153 Cuts at: 3220 3238 3257 3311 3392 3689 3715 3788 3857 Size: 18 19 54 81 297 26 73 69 Cuts at: 3857 2101 Size: 5016 Fragments arranged by size: 5016 297 276 179 162 162 153 106 81 73 69 64 54 26 19 18 17 PspXI vC'TCGA_Gb Cuts at: 3126 3495 3126 Size: 369 6403 PstI C_TGCA'G Cuts at: 830 2571 4124 830 Size: 1741 1553 3478 Fragments arranged by size: 3478 1741 1553 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6772 SacII CC_GC'GG Cuts at: 3319 3319 Size: 6772 SalI G'TCGA_C Cuts at: 1936 3954 4114 1936 Size: 2018 160 4594 Fragments arranged by size: 4594 2018 160 SanDI GG'GwC_CC Cuts at: 2111 2192 2425 2636 2690 2934 3927 2111 Size: 81 233 211 54 244 993 4956 Fragments arranged by size: 4956 993 244 233 211 81 54 SbfI CC_TGCA'GG Cuts at: 4124 4124 Size: 6772 ScaI AGT'ACT Cuts at: 5020 6732 5020 Size: 1712 5060 SexAI A'CCwGG_T Cuts at: 2473 2473 Size: 6772 SfcI C'TryA_G Cuts at: 826 1045 2567 4120 5255 5936 6127 826 Size: 219 1522 1553 1135 681 191 1471 Fragments arranged by size: 1553 1522 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2227 2343 3075 3891 3969 2227 Size: 116 732 816 78 5030 Fragments arranged by size: 5030 816 732 116 78 SfoI GGC'GCC Cuts at: 3457 3457 Size: 6772 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6772 SmaI CCC'GGG Cuts at: 1872 2109 2118 2245 2317 2484 3535 3847 4099 Size: 237 9 127 72 167 1051 312 252 Cuts at: 4099 1872 Size: 4545 Fragments arranged by size: 4545 1051 312 252 237 167 127 72 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 3126 3495 4876 5777 6039 820 Size: 197 926 1183 369 1381 901 262 1553 Fragments arranged by size: 1553 1381 1183 926 901 369 262 197 SnaBI TAC'GTA Cuts at: 493 4563 493 Size: 4070 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6772 SphI G_CATG'C Cuts at: 1132 2588 4130 1132 Size: 1456 1542 3774 Fragments arranged by size: 3774 1542 1456 SrfI GCCC'GGGC Cuts at: 2317 2317 Size: 6772 SspI AAT'ATT Cuts at: 5 52 4696 5 Size: 47 4644 2081 Fragments arranged by size: 4644 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6772 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 2268 2286 2418 2640 2730 Size: 552 939 192 72 18 132 222 90 Cuts at: 2730 2810 3172 3262 3396 3721 3792 4012 4211 Size: 80 362 90 134 325 71 220 199 Cuts at: 4211 513 Size: 3074 Fragments arranged by size: 3074 939 552 362 325 222 220 199 192 134 132 90 90 80 72 71 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2597 2642 3317 3641 4799 4816 4969 Size: 1255 1017 45 675 324 1158 17 153 Cuts at: 4969 5154 5599 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1158 1017 675 445 324 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5018 6730 96 Size: 274 80 33 51 1443 3041 1712 138 Fragments arranged by size: 3041 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2507 2539 2593 2625 6732 6764 2507 Size: 32 54 32 4107 32 2515 Fragments arranged by size: 4107 2515 54 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6772 XbaI T'CTAG_A Cuts at: 4108 4108 Size: 6772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2647 3728 2497 Size: 150 1081 5541 Fragments arranged by size: 5541 1081 150 XhoI C'TCGA_G Cuts at: 1943 3126 3495 1943 Size: 1183 369 5220 Fragments arranged by size: 5220 1183 369 XmaI C'CCGG_G Cuts at: 1870 2107 2116 2243 2315 2482 3533 3845 4097 Size: 237 9 127 72 167 1051 312 252 Cuts at: 4097 1870 Size: 4545 Fragments arranged by size: 4545 1051 312 252 237 167 127 72 9 XmnI GAAnn'nnTTC Cuts at: 4901 4901 Size: 6772 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4580 276 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SapI SwaI