(Linear) (Six-base) MAPSORT of: KIEE1179.seq Check: 9151 from: 1 to: 2067 KIEE1179.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AccI GT'mk_AC Cuts at: 0 1950 2067 Size: 1950 117 Acc65I G'GTAC_C Cuts at: 0 1694 2067 Size: 1694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1609 2067 Size: 1609 458 AlwNI CAG_nnn'CTG Cuts at: 0 495 2047 2067 Size: 495 1552 20 Fragments arranged by size: 1552 495 20 ApaI G_GGCC'C Cuts at: 0 100 164 343 449 725 742 904 1066 Size: 100 64 179 106 276 17 162 162 Cuts at: 1066 1219 1237 1256 1310 1391 1688 1714 1787 Size: 153 18 19 54 81 297 26 73 Cuts at: 1787 1856 2067 Size: 69 211 Fragments arranged by size: 297 276 211 179 162 162 153 106 100 81 73 69 64 54 26 19 18 17 ApoI r'AATT_y Cuts at: 0 1244 2067 Size: 1244 823 AvaI C'yCGr_G Cuts at: 0 102 111 238 310 477 1121 1490 1528 Size: 102 9 127 72 167 644 369 38 Cuts at: 1528 1733 1840 2067 Size: 205 107 227 Fragments arranged by size: 644 369 227 205 167 127 107 102 72 38 9 AvrII C'CTAG_G Cuts at: 0 805 2067 Size: 805 1262 BamHI G'GATC_C Cuts at: 0 133 287 1862 2050 2067 Size: 133 154 1575 188 17 Fragments arranged by size: 1575 188 154 133 17 BanI G'GyrC_C Cuts at: 0 649 775 1450 1694 1721 1835 2067 Size: 649 126 675 244 27 114 232 Fragments arranged by size: 675 649 244 232 126 114 27 BanII G_rGCy'C Cuts at: 0 100 164 343 449 725 742 904 1066 Size: 100 64 179 106 276 17 162 162 Cuts at: 1066 1219 1237 1256 1291 1310 1391 1688 1714 Size: 153 18 19 35 19 81 297 26 Cuts at: 1714 1787 1856 1935 2067 Size: 73 69 79 132 Fragments arranged by size: 297 276 179 162 162 153 132 106 100 81 79 73 69 64 35 26 19 19 18 17 BbeI G_GCGC'C Cuts at: 0 1454 2067 Size: 1454 613 BbsI GAAGACnn'nnnn_ Cuts at: 0 1306 2067 Size: 1306 761 BglI GCCn_nnn'nGGC Cuts at: 0 13 222 338 908 998 1070 1736 1886 Size: 13 209 116 570 90 72 666 150 Cuts at: 1886 1964 2067 Size: 78 103 Fragments arranged by size: 666 570 209 150 116 103 90 78 72 13 Bme1580I G_kGCm'C Cuts at: 0 100 164 343 449 725 742 904 1066 Size: 100 64 179 106 276 17 162 162 Cuts at: 1066 1219 1237 1256 1310 1391 1688 1714 1724 Size: 153 18 19 54 81 297 26 10 Cuts at: 1724 1787 1838 1856 2067 Size: 63 51 18 211 Fragments arranged by size: 297 276 211 179 162 162 153 106 100 81 64 63 54 51 26 19 18 18 17 10 BmrI ACTGGGnnnn_n' Cuts at: 0 791 2067 Size: 791 1276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 278 314 677 1064 1655 1919 2067 Size: 28 250 36 363 387 591 264 148 Fragments arranged by size: 591 387 363 264 250 148 36 28 BsaI GGTCTCn'nnnn_ Cuts at: 0 1040 2067 Size: 1040 1027 BsaAI yAC'GTr Cuts at: 0 1411 2067 Size: 1411 656 BsaBI GATnn'nnATC Cuts at: 0 400 1913 2067 Size: 400 1513 154 Fragments arranged by size: 1513 400 154 BsaHI Gr'CG_yC Cuts at: 0 1271 1451 2067 Size: 1271 180 616 Fragments arranged by size: 1271 616 180 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1273 1303 2067 Size: 1273 30 764 Fragments arranged by size: 1273 764 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 31 1139 2067 Size: 31 1108 928 Fragments arranged by size: 1108 928 31 BseYI C'CCAG_C Cuts at: 0 211 826 1435 2067 Size: 211 615 609 632 Fragments arranged by size: 632 615 609 211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 980 2067 Size: 980 1087 BsiHKAI G_wGCw'C Cuts at: 0 59 2067 Size: 59 2008 BsmBI CGTCTCn'nnnn_ Cuts at: 0 727 2067 Size: 727 1340 Bsp1286I G_dGCh'C Cuts at: 0 59 100 164 343 449 725 742 904 Size: 59 41 64 179 106 276 17 162 Cuts at: 904 1066 1219 1237 1256 1291 1310 1391 1688 Size: 162 153 18 19 35 19 81 297 Cuts at: 1688 1714 1724 1787 1838 1856 1935 2067 Size: 26 10 63 51 18 79 132 Fragments arranged by size: 297 276 179 162 162 153 132 106 81 79 64 63 59 51 41 35 26 19 19 18 18 17 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1936 2004 2067 Size: 1936 68 63 Fragments arranged by size: 1936 68 63 BsrBI CCG'CTC Cuts at: 0 93 2067 Size: 93 1974 BsrFI r'CCGG_y Cuts at: 0 1339 1880 2067 Size: 1339 541 187 Fragments arranged by size: 1339 541 187 BstEII G'GTnAC_C Cuts at: 0 1162 1324 2067 Size: 1162 162 743 Fragments arranged by size: 1162 743 162 BstYI r'GATC_y Cuts at: 0 133 287 1862 2050 2067 Size: 133 154 1575 188 17 Fragments arranged by size: 1575 188 154 133 17 Bsu36I CC'TnA_GG Cuts at: 0 1202 1220 2067 Size: 1202 18 847 Fragments arranged by size: 1202 847 18 BtgI C'CryG_G Cuts at: 0 281 1311 1716 1760 1970 2067 Size: 281 1030 405 44 210 97 Fragments arranged by size: 1030 405 281 210 97 44 BtsI GCAGTG_nn' Cuts at: 0 510 963 2067 Size: 510 453 1104 Fragments arranged by size: 1104 510 453 DraIII CAC_nnn'GTG Cuts at: 0 793 1575 2067 Size: 793 782 492 Fragments arranged by size: 793 782 492 DrdI GACnn_nn'nnGTC Cuts at: 0 683 2067 Size: 683 1384 EaeI y'GGCC_r Cuts at: 0 2 612 1337 1499 1878 1887 2067 Size: 2 610 725 162 379 9 180 Fragments arranged by size: 725 610 379 180 162 9 2 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 278 314 677 1064 1609 1655 1919 Size: 28 250 36 363 387 545 46 264 Cuts at: 1919 2067 Size: 148 Fragments arranged by size: 545 387 363 264 250 148 46 36 28 EcoO109I rG'GnC_Cy Cuts at: 0 96 97 106 161 187 340 409 420 Size: 96 1 9 55 26 153 69 11 Cuts at: 420 445 446 454 550 631 685 721 739 Size: 25 1 8 96 81 54 36 18 Cuts at: 739 802 901 929 1039 1071 1117 1198 1215 Size: 63 99 28 110 32 46 81 17 Cuts at: 1215 1216 1233 1234 1252 1253 1307 1387 1666 Size: 1 17 1 18 1 54 80 279 Cuts at: 1666 1675 1685 1710 1711 1783 1853 1922 1966 Size: 9 10 25 1 72 70 69 44 Cuts at: 1966 2062 2067 Size: 96 5 Fragments arranged by size: 279 153 110 99 96 96 96 81 81 80 72 70 69 69 63 55 54 54 46 44 36 32 28 26 25 25 18 18 17 17 11 10 9 9 8 5 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 0 1244 2067 Size: 1244 823 FseI GG_CCGG'CC Cuts at: 0 1343 1884 2067 Size: 1343 541 183 Fragments arranged by size: 1343 541 183 HaeII r_GCGC'y Cuts at: 0 770 1454 1588 2067 Size: 770 684 134 479 Fragments arranged by size: 770 684 479 134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 63 95 424 456 726 758 1273 1305 Size: 63 32 329 32 270 32 515 32 Cuts at: 1305 1653 1685 1906 1938 2035 2067 Size: 348 32 221 32 97 32 Fragments arranged by size: 515 348 329 270 221 97 63 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1951 2067 Size: 1951 116 KasI G'GCGC_C Cuts at: 0 1450 2067 Size: 1450 617 KpnI G_GTAC'C Cuts at: 0 1698 2067 Size: 1698 369 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 161 547 2067 Size: 161 386 1520 Fragments arranged by size: 1520 386 161 MscI TGG'CCA Cuts at: 0 614 1889 2067 Size: 614 1275 178 Fragments arranged by size: 1275 614 178 MspA1I CmG'CkG Cuts at: 0 1313 1439 2067 Size: 1313 126 628 Fragments arranged by size: 1313 628 126 NaeI GCC'GGC Cuts at: 0 1341 1882 2067 Size: 1341 541 185 Fragments arranged by size: 1341 541 185 NarI GG'CG_CC Cuts at: 0 1451 2067 Size: 1451 616 NcoI C'CATG_G Cuts at: 0 281 1716 2067 Size: 281 1435 351 Fragments arranged by size: 1435 351 281 NgoMIV G'CCGG_C Cuts at: 0 1339 1880 2067 Size: 1339 541 187 Fragments arranged by size: 1339 541 187 NspI r_CATG'y Cuts at: 0 583 2067 Size: 583 1484 PasI CC'CwG_GG Cuts at: 0 460 556 690 870 1096 1140 1294 1330 Size: 460 96 134 180 226 44 154 36 Cuts at: 1330 1456 1644 1690 1858 1927 2067 Size: 126 188 46 168 69 140 Fragments arranged by size: 460 226 188 180 168 154 140 134 126 96 69 46 44 36 PflMI CCAn_nnn'nTGG Cuts at: 0 495 2067 Size: 495 1572 PfoI T'CCnGG_A Cuts at: 0 183 369 1077 1671 1815 1935 2067 Size: 183 186 708 594 144 120 132 Fragments arranged by size: 708 594 186 183 144 132 120 PpuMI rG'GwC_Cy Cuts at: 0 106 187 420 454 550 631 685 802 Size: 106 81 233 34 96 81 54 117 Cuts at: 802 929 1666 1675 1922 2067 Size: 127 737 9 247 145 Fragments arranged by size: 737 247 233 145 127 117 106 96 81 81 54 34 9 PspOMI G'GGCC_C Cuts at: 0 96 160 339 445 721 738 900 1062 Size: 96 64 179 106 276 17 162 162 Cuts at: 1062 1215 1233 1252 1306 1387 1684 1710 1783 Size: 153 18 19 54 81 297 26 73 Cuts at: 1783 1852 2067 Size: 69 215 Fragments arranged by size: 297 276 215 179 162 162 153 106 96 81 73 69 64 54 26 19 18 17 PspXI vC'TCGA_Gb Cuts at: 0 1121 1490 2067 Size: 1121 369 577 Fragments arranged by size: 1121 577 369 PstI C_TGCA'G Cuts at: 0 566 2067 Size: 566 1501 SacII CC_GC'GG Cuts at: 0 1314 2067 Size: 1314 753 SalI G'TCGA_C Cuts at: 0 1949 2067 Size: 1949 118 SanDI GG'GwC_CC Cuts at: 0 106 187 420 631 685 929 1922 2067 Size: 106 81 233 211 54 244 993 145 Fragments arranged by size: 993 244 233 211 145 106 81 54 SexAI A'CCwGG_T Cuts at: 0 468 2067 Size: 468 1599 SfcI C'TryA_G Cuts at: 0 562 2067 Size: 562 1505 SfiI GGCCn_nnn'nGGCC Cuts at: 0 222 338 1070 1886 1964 2067 Size: 222 116 732 816 78 103 Fragments arranged by size: 816 732 222 116 103 78 SfoI GGC'GCC Cuts at: 0 1452 2067 Size: 1452 615 SmaI CCC'GGG Cuts at: 0 104 113 240 312 479 1530 1842 2067 Size: 104 9 127 72 167 1051 312 225 Fragments arranged by size: 1051 312 225 167 127 104 72 9 SmlI C'TyrA_G Cuts at: 0 1121 1490 2067 Size: 1121 369 577 Fragments arranged by size: 1121 577 369 SphI G_CATG'C Cuts at: 0 583 2067 Size: 583 1484 SrfI GCCC'GGGC Cuts at: 0 312 2067 Size: 312 1755 StyI C'CwwG_G Cuts at: 0 191 263 281 413 635 725 805 1167 Size: 191 72 18 132 222 90 80 362 Cuts at: 1167 1257 1391 1716 1787 2007 2067 Size: 90 134 325 71 220 60 Fragments arranged by size: 362 325 222 220 191 134 132 90 90 80 72 71 60 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 592 637 1312 1636 2067 Size: 592 45 675 324 431 Fragments arranged by size: 675 592 431 324 45 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 502 534 588 620 2067 Size: 502 32 54 32 1447 Fragments arranged by size: 1447 502 54 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 492 642 1723 2067 Size: 492 150 1081 344 Fragments arranged by size: 1081 492 344 150 XhoI C'TCGA_G Cuts at: 0 1121 1490 2067 Size: 1121 369 577 Fragments arranged by size: 1121 577 369 XmaI C'CCGG_G Cuts at: 0 102 111 238 310 477 1528 1840 2067 Size: 102 9 127 72 167 1051 312 227 Fragments arranged by size: 1051 312 227 167 127 102 72 9 Enzymes that do cut: AccI Acc65I AcuI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI Eco57MI EcoO109I EcoRI FseI HaeII Hin4I HincII KasI KpnI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PspXI PstI SacII SalI SanDI SexAI SfcI SfiI SfoI SmaI SmlI SphI SrfI StyI TaqII TstI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EarI EciI EcoICRI EcoNI EcoRV FalI FspI FspAI HindIII HpaI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI Tth111I XbaI XmnI ZraI