################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI AspEI AspI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsiYI BslI BsmFI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI Ksp632I KspI MaeI MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 229 GGTACC G^GTACC AciI 246 GCGG CCGC(-3/-1) AluI 254 AGCT AG^CT Alw44I 128 GTGCAC G^TGCAC ApaLI 128 GTGCAC G^TGCAC Asp700I 21 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 229 GGTACC G^GTACC AspHI 128 GTGCAC G[AT]GC[AT]^C AvaI 149 CTCGGG C^[CT]CG[GA]G BanI 229 GGTACC G^G[CT][GA]CC BbvI 243 GCAGC GCAGC(8/12) BmyI 128 GTGCAC G[AGT]GC[ACT]^C BsaJI 310 CCTCGG C^C[ACGT][ACGT]GG BsgI 224 GTGCAG GTGCAG(16/14) BsiHKAI 128 GTGCAC G[AT]GC[AT]^C BsmAI 219 GAGAC GTCTC(1/5) BsmBI 219 GAGACG CGTCTC(1/5) BsoBI 149 CTCGGG C^[CT]CG[GA]G Bsp1286I 128 GTGCAC G[AGT]GC[ACT]^C BspDI 44 ATCGAT AT^CGAT BspMI 226 GCAGGT ACCTGC(4/8) BstF5I 15 CATCC GGATG(2/0) Cfr13I 314 GGCCC G^G[ACGT]CC ClaI 44 ATCGAT AT^CGAT DpnII 43 GATC ^GATC FauI 246 GCGGG CCCGC(4/6) Fnu4HI 243 GCAGC GC^[ACGT]GC FokI 15 CATCC GGATG(9/13) HincII 51 GTTGAC GT[CT]^[GA]AC HindII 51 GTTGAC GT[CT]^[GA]AC HinfI 137 GAATC G^A[ACGT]TC ItaI 243 GCAGC GC^[ACGT]GC KpnI 229 GGTACC GGTAC^C MaeII 222 ACGT A^CGT MboI 43 GATC ^GATC MseI 35 TTAA T^TAA MspA1I 244 CAGCGG C[AC]G^C[GT]G MwoI 246 GCGGGATAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 43 GATC ^GATC Sau3AI 43 GATC ^GATC Sau96I 314 GGCCC G^G[ACGT]CC SfaNI 95 GATGC GCATC(5/9) TfiI 137 GAATC G^A[AT]TC Tru9I 35 TTAA T^TAA TseI 243 GCAGC G^C[AT]GC XmnI 21 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AfaI 230 GTAC GT^AC 273 GTAC HaeIII 31 GGCC GG^CC 314 GGCC HphI 67 GGTGA GGTGA(8/7) 288 TCACC NlaIII 100 CATG CATG^ 133 CATG NlaIV 229 GGTACC GG[ACGT]^[ACGT]CC 306 GGAGCC RsaI 230 GTAC GT^AC 273 GTAC TaqI 45 TCGA T^CGA 93 TCGA TthHB8I 45 TCGA T^CGA 93 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### MnlI