################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwI AlwNI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CfoI ClaI CpoI DpnII DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### Alw44I 149 GTGCAC G^TGCAC AocI 207 CCTCAGG CC^T[ACGT]AGG ApaI 276 GGGCCC GGGCC^C ApaLI 149 GTGCAC G^TGCAC BbvCI 328 GCTGAGG CCTCAGC(-5/-2) BcgI 683 CGACCAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 480 GCTTAGC GC^T[ACGT]AGC Bpu1102I 480 GCTTAGC GC^T[ACGT]AGC BsgI 436 GTGCAG GTGCAG(16/14) BsrFI 131 ACCGGC [GA]^CCGG[CT] BsrI 587 ACTGG ACTGG(1/-1) Bsu36I 207 CCTCAGG CC^T[ACGT]AGG CelII 480 GCTTAGC GC^T[ACGT]AGC Cfr10I 131 ACCGGC [GA]^CCGG[CT] DraIII 586 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 207 CCTCAGG CC^T[ACGT]AGG FauI 52 CCCGC CCCGC(4/6) HincII 730 GTTGAC GT[CT]^[GA]AC HindII 730 GTTGAC GT[CT]^[GA]AC MaeIII 313 GTGAC ^GT[ACGT]AC NspI 755 ACATGC [GA]CATG^[CT] PleI 460 GAGTC GAGTC(4/5) PpuMI 698 GGGACCT [GA]G^G[AT]CC[CT] PshAI 642 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 232 CAGCTG CAG^CTG SacI 75 GAGCTC GAGCT^C SfaNI 307 GATGC GCATC(5/9) TfiI 360 GATTC G^A[AT]TC Tsp45I 313 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BalI 188 TGGCCA TGG^CCA 396 TGGCCA BcnI 168 CCGGG CC^[GC]GG 336 CCCGG BseRI 652 CTCCTC GAGGAG(10/8) 370 GAGGAG BstXI 191 CCAAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 220 CCATCCTTCTGG EaeI 188 TGGCCA [CT]^GGCC[GA] 396 TGGCCA EcoNI 203 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 570 CCTGCAGCAGG HinfI 360 GATTC G^A[ACGT]TC 460 GAGTC MluNI 188 TGGCCA TGG^CCA 396 TGGCCA MscI 188 TGGCCA TGG^CCA 396 TGGCCA NciI 168 CCGGG CC^[GC]GG 336 CCCGG NlaIII 29 CATG CATG^ 756 CATG PstI 262 CTGCAG CTGCA^G 571 CTGCAG SfcI 262 CTGCAG C^T[GA][CT]AG 571 CTGCAG StuI 173 AGGCCT AGG^CCT 540 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AspHI AvaII BanI BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MwoI NlaIV Sau96I ScrFI SmlI StyI TseI TspRI