################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BalI BamHI BanI BbeI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaBI BsaHI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau3AI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 720 GTAC GT^AC ApaI 739 GGGCCC GGGCC^C Asp700I 225 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 266 TGCGCA TGC^GCA BbrPI 79 CACGTG CAC^GTG BcgI 758 GCAGCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 472 CTAG C^TAG BlpI 808 GCTCAGC GC^T[ACGT]AGC Bpu1102I 808 GCTCAGC GC^T[ACGT]AGC BsaAI 79 CACGTG [CT]AC^GT[GA] BsaI 645 GGTCTC GGTCTC(1/5) BsiEI 484 CGGCCG CG[GA][CT]^CG BsmAI 646 GTCTC GTCTC(1/5) BspHI 149 TCATGA T^CATGA CelII 808 GCTCAGC GC^T[ACGT]AGC CfoI 267 GCGC GCG^C DrdI 376 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 484 CGGCCG C^GGCCG EclXI 484 CGGCCG C^GGCCG Eco52I 484 CGGCCG C^GGCCG EcoNI 174 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 194 CCAAGG C^C[AT][AT]GG FspI 266 TGCGCA TGC^GCA HhaI 267 GCGC GCG^C HinP1I 267 GCGC G^CGC HinfI 746 GAGTC G^A[ACGT]TC MaeI 472 CTAG C^TAG MaeII 80 ACGT A^CGT MslI 75 CACGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PleI 746 GAGTC GAGTC(4/5) PmaCI 79 CACGTG CAC^GTG PmlI 79 CACGTG CAC^GTG PpuMI 134 GGGACCT [GA]G^G[AT]CC[CT] PshAI 639 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 120 CTGCAG CTGCA^G RcaI 149 TCATGA T^CATGA RsaI 720 GTAC GT^AC ScaI 719 AGTACT AGT^ACT SfaNI 4 GATGC GCATC(5/9) SfcI 120 CTGCAG C^T[GA][CT]AG SmaI 671 CCCGGG CCC^GGG SmlI 421 CTCAAG C^T[CT][GA]AG StuI 794 AGGCCT AGG^CCT StyI 194 CCAAGG C^C[AT][AT]GG Tsp509I 306 AATT ^AATT TspRI 619 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 17 CCAAAGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 671 CCCGGG C^CCGGG XmaI 671 CCCGGG C^CCGGG XmnI 225 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 94 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 123 CAGCCTCTG BcnI 671 CCCGG CC^[GC]GG 672 CCGGG BpmI 64 CTGGAG CTGGAG(16/14) 350 CTGGAG BsgI 534 CTGCAC GTGCAG(16/14) 553 GTGCAG DdeI 660 CTCAG C^T[ACGT]AG 809 CTCAG DraII 134 GGGACCT [GA]G^G[ACGT]CC[CT] 739 GGGCCCC DsaI 212 CCGTGG C^C[GA][CT]GG 357 CCACGG EaeI 215 TGGCCG [CT]^GGCC[GA] 484 CGGCCG EarI 691 CTCTTC CTCTTC(1/4) 283 GAAGAG EcoO109I 134 GGGACCT [GA]G^G[ACGT]CC[CT] 739 GGGCCCC HphI 191 TCACC GGTGA(8/7) 749 TCACC Ksp632I 691 CTCTTC CTCTTC(1/4) 283 GAAGAG MseI 38 TTAA T^TAA 500 TTAA NciI 671 CCCGG CC^[GC]GG 672 CCGGG TaqI 143 TCGA T^CGA 593 TCGA Tru9I 38 TTAA T^TAA 500 TTAA TthHB8I 143 TCGA T^CGA 593 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AvaI AvaII BanII BbvI BmyI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV PflMI PvuII Sau96I ScrFI TseI Tsp45I Van91I