Restriction map for
pFN21AE1815
(Circular) (Six-base) MAPSORT of: pFN21AE1815.seq Check: 8522 from: 1 to: 5698
pFN21AE1815 5698 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2239 2673 3041 1937
Size: 302 434 368 4594
Fragments arranged by size:
4594 434 368 302
Acc65I G'GTAC_C
Cuts at: 2029 3019 2029
Size: 990 4708
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 4597 5574 1203
Size: 3394 977 1327
Fragments arranged by size:
3394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 2210 1166
Size: 1044 4654
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 2039 2515 2578 3441 5013 2039
Size: 476 63 863 1572 2724
Fragments arranged by size:
2724 1572 863 476 63
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2665 2698 2665
Size: 33 5665
BamHI G'GATC_C
Cuts at: 2989 3028 3395 2989
Size: 39 367 5292
Fragments arranged by size:
5292 367 39
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 2204 3019 4474 618
Size: 325 217 627 242 175 815 1455 1842
Fragments arranged by size:
1842 1455 815 627 325 242 217 175
BanII G_rGCy'C
Cuts at: 1391 2883 3017 1391
Size: 1492 134 4072
Fragments arranged by size:
4072 1492 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 2808 2852 928
Size: 971 231 678 44 3774
Fragments arranged by size:
3774 971 678 231 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2404 5693 2404
Size: 3289 2409
BlpI GC'TnA_GC
Cuts at: 2762 3115 2762
Size: 353 5345
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2842 3429 4387
Size: 416 53 83 399 24 1425 587 958
Cuts at: 4387 442
Size: 1753
Fragments arranged by size:
1753 1425 958 587 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 2576 2739 4358 1239
Size: 1187 150 163 1619 2579
Fragments arranged by size:
2579 1619 1187 163 150
Bpu10I CC'TnA_GC
Cuts at: 2478 2973 2478
Size: 495 5203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2687 4361 882
Size: 617 1188 1674 2219
Fragments arranged by size:
2219 1674 1188 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 3505 3887 4522
Size: 53 83 186 936 489 1483 382 635
Cuts at: 4522 275
Size: 1451
Fragments arranged by size:
1483 1451 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2049 2922 4131 4529 4803 4950 5361 2049
Size: 873 1209 398 274 147 411 2386
Fragments arranged by size:
2386 1209 873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5473
Fragments arranged by size:
5473 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2447 2672 2689 1186
Size: 960 301 225 17 4195
Fragments arranged by size:
4195 960 301 225 17
BseYI C'CCAG_C
Cuts at: 4901 5397 4901
Size: 496 5202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2718 1163
Size: 573 982 4143
Fragments arranged by size:
4143 982 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2883 3759 3844 4915 2883
Size: 876 85 1071 3666
Fragments arranged by size:
3666 1071 876 85
BsmI GAATG_Cn'
Cuts at: 2914 3209 3302 2914
Size: 295 93 5310
Fragments arranged by size:
5310 295 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2883 3017 3759 3844 4915 1391
Size: 399 1093 134 742 85 1071 2174
Fragments arranged by size:
2174 1093 1071 742 399 134 85
BspEI T'CCGG_A
Cuts at: 2049 2922 5361 2049
Size: 873 2439 2386
Fragments arranged by size:
2439 2386 873
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2970 3053 844
Size: 2126 83 3489
Fragments arranged by size:
3489 2126 83
BsrBI CCG'CTC
Cuts at: 1394 1823 2875 3585 1394
Size: 429 1052 710 3507
Fragments arranged by size:
3507 1052 710 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2578 2911 794
Size: 1784 333 3581
Fragments arranged by size:
3581 1784 333
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 2011 2791 2957 1184
Size: 827 780 166 3925
Fragments arranged by size:
3925 827 780 166
BstXI CCAn_nnnn'nTGG
Cuts at: 2815 2815
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2046 2404 2989 3028 3395 3780 3797 5249
Size: 74 358 585 39 367 385 17 1452
Cuts at: 5249 5335 5347 5693 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 585 385 367 358 346 86
74 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2129 2129
Size: 5698
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DraIII CAC_nnn'GTG
Cuts at: 2104 2957 2104
Size: 853 4845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2154 2385
Size: 54 1425 50 275 18 276 48 231
Cuts at: 2385 3145 4034 5663 8
Size: 760 889 1629 43
Fragments arranged by size:
1629 1425 889 760 276 275 231 54
50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2628 3628 1353
Size: 1275 1000 3423
Fragments arranged by size:
3423 1275 1000
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2881 2881
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 2576 2739 3761 4358 5145 5413
Size: 393 794 150 163 1022 597 787 268
Cuts at: 5413 1239
Size: 1524
Fragments arranged by size:
1524 1022 794 787 597 393 268 163
150
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2429 2477 2195
Size: 234 48 5416
Fragments arranged by size:
5416 234 48
EcoO109I rG'GnC_Cy
Cuts at: 2437 2437
Size: 5698
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 4339 4371 4413
Size: 32 135 32 466 32 2269 32 42
Cuts at: 4413 4445 1373
Size: 32 2626
Fragments arranged by size:
2626 2269 466 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2455 3042 3288 669
Size: 988 281 517 587 246 3079
Fragments arranged by size:
3079 988 587 517 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 2033 3023 2033
Size: 990 4708
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2554 4812 4996 1305
Size: 1249 2258 184 2007
Fragments arranged by size:
2258 2007 1249 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 2387 10
Size: 54 1425 343 324 231 3321
Fragments arranged by size:
3321 1425 343 324 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2210 3656 4015 4174 518
Size: 648 843 201 1446 359 159 2042
Fragments arranged by size:
2042 1446 843 648 359 201 159
MspA1I CmG'CkG
Cuts at: 1606 2358 2395 2512 2584 3114 3791 4939 5184
Size: 752 37 117 72 530 677 1148 245
Cuts at: 5184 1606
Size: 2120
Fragments arranged by size:
2120 1148 752 677 530 245 117 72
37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 3056 4601 1132
Size: 97 1827 1545 2229
Fragments arranged by size:
2229 1827 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2039 2599 1108
Size: 931 560 4207
Fragments arranged by size:
4207 931 560
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2437 2437
Size: 5698
PshAI GACnn'nnGTC
Cuts at: 1613 2177 1613
Size: 564 5134
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 2520 2583 2898 2967 3050 830
Size: 1690 63 315 69 83 3478
Fragments arranged by size:
3478 1690 315 83 69 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
PvuII CAG'CTG
Cuts at: 2395 2584 2395
Size: 189 5509
SacI G_AGCT'C
Cuts at: 2883 2883
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SexAI A'CCwGG_T
Cuts at: 2953 2953
Size: 5698
SfcI C'TryA_G
Cuts at: 826 1045 2083 2516 2579 2894 2963 3046 4181
Size: 219 1038 433 63 315 69 83 1135
Cuts at: 4181 4862 5053 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1038 681 433 315 219 191
83 69 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3802 4703 4965 820
Size: 197 926 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 2141 2867 2945 1517
Size: 624 726 78 4270
Fragments arranged by size:
4270 726 624 78
StyI C'CwwG_G
Cuts at: 513 1065 2004 2796 3137 513
Size: 552 939 792 341 3074
Fragments arranged by size:
3074 939 792 552 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3944 5656 96
Size: 274 80 33 51 1443 1967 1712 138
Fragments arranged by size:
1967 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI