(Circular) (Six-base) MAPSORT of: pFN21AE1815.seq Check: 8522 from: 1 to: 5698 pFN21AE1815 5698 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2239 2673 3041 1937 Size: 302 434 368 4594 Fragments arranged by size: 4594 434 368 302 Acc65I G'GTAC_C Cuts at: 2029 3019 2029 Size: 990 4708 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 5145 5413 1632 Size: 2129 1384 268 1917 Fragments arranged by size: 2129 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 2210 1166 Size: 1044 4654 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2039 2515 2578 3441 5013 2039 Size: 476 63 863 1572 2724 Fragments arranged by size: 2724 1572 863 476 63 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2665 2698 2665 Size: 33 5665 BamHI G'GATC_C Cuts at: 2989 3028 3395 2989 Size: 39 367 5292 Fragments arranged by size: 5292 367 39 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2204 3019 4474 618 Size: 325 217 627 242 175 815 1455 1842 Fragments arranged by size: 1842 1455 815 627 325 242 217 175 BanII G_rGCy'C Cuts at: 1391 2883 3017 1391 Size: 1492 134 4072 Fragments arranged by size: 4072 1492 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 2808 2852 928 Size: 971 231 678 44 3774 Fragments arranged by size: 3774 971 678 231 44 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2404 5693 2404 Size: 3289 2409 BlpI GC'TnA_GC Cuts at: 2762 3115 2762 Size: 353 5345 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2842 3429 4387 Size: 416 53 83 399 24 1425 587 958 Cuts at: 4387 442 Size: 1753 Fragments arranged by size: 1753 1425 958 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2716 2716 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2426 2576 2739 4358 1239 Size: 1187 150 163 1619 2579 Fragments arranged by size: 2579 1619 1187 163 150 Bpu10I CC'TnA_GC Cuts at: 2478 2973 2478 Size: 495 5203 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2687 4361 882 Size: 617 1188 1674 2219 Fragments arranged by size: 2219 1674 1188 617 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 3505 3887 4522 Size: 53 83 186 936 489 1483 382 635 Cuts at: 4522 275 Size: 1451 Fragments arranged by size: 1483 1451 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2049 2922 4131 4529 4803 4950 5361 2049 Size: 873 1209 398 274 147 411 2386 Fragments arranged by size: 2386 1209 873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5473 Fragments arranged by size: 5473 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 2447 2672 2689 1186 Size: 960 301 225 17 4195 Fragments arranged by size: 4195 960 301 225 17 BseYI C'CCAG_C Cuts at: 4901 5397 4901 Size: 496 5202 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2718 1163 Size: 573 982 4143 Fragments arranged by size: 4143 982 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2883 3759 3844 4915 2883 Size: 876 85 1071 3666 Fragments arranged by size: 3666 1071 876 85 BsmI GAATG_Cn' Cuts at: 2914 3209 3302 2914 Size: 295 93 5310 Fragments arranged by size: 5310 295 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2883 3017 3759 3844 4915 1391 Size: 399 1093 134 742 85 1071 2174 Fragments arranged by size: 2174 1093 1071 742 399 134 85 BspEI T'CCGG_A Cuts at: 2049 2922 5361 2049 Size: 873 2439 2386 Fragments arranged by size: 2439 2386 873 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2970 3053 844 Size: 2126 83 3489 Fragments arranged by size: 3489 2126 83 BsrBI CCG'CTC Cuts at: 1394 1823 2875 3585 1394 Size: 429 1052 710 3507 Fragments arranged by size: 3507 1052 710 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 2578 2911 794 Size: 1784 333 3581 Fragments arranged by size: 3581 1784 333 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 2011 2791 2957 1184 Size: 827 780 166 3925 Fragments arranged by size: 3925 827 780 166 BstXI CCAn_nnnn'nTGG Cuts at: 2815 2815 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2046 2404 2989 3028 3395 3780 3797 5249 Size: 74 358 585 39 367 385 17 1452 Cuts at: 5249 5335 5347 5693 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 585 385 367 358 346 86 74 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2129 2129 Size: 5698 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DraIII CAC_nnn'GTG Cuts at: 2104 2957 2104 Size: 853 4845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2154 2385 Size: 54 1425 50 275 18 276 48 231 Cuts at: 2385 3145 4034 5663 8 Size: 760 889 1629 43 Fragments arranged by size: 1629 1425 889 760 276 275 231 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2628 3628 1353 Size: 1275 1000 3423 Fragments arranged by size: 3423 1275 1000 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2881 2881 Size: 5698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2426 2576 2739 3761 4358 5145 5413 Size: 393 794 150 163 1022 597 787 268 Cuts at: 5413 1239 Size: 1524 Fragments arranged by size: 1524 1022 794 787 597 393 268 163 150 EcoNI CCTnn'n_nnAGG Cuts at: 2195 2429 2477 2195 Size: 234 48 5416 Fragments arranged by size: 5416 234 48 EcoO109I rG'GnC_Cy Cuts at: 2437 2437 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2038 2070 4339 4371 4413 Size: 32 135 32 466 32 2269 32 42 Cuts at: 4413 4445 1373 Size: 32 2626 Fragments arranged by size: 2626 2269 466 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2455 3042 3288 669 Size: 988 281 517 587 246 3079 Fragments arranged by size: 3079 988 587 517 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 2033 3023 2033 Size: 990 4708 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2554 4812 4996 1305 Size: 1249 2258 184 2007 Fragments arranged by size: 2258 2007 1249 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 2387 10 Size: 54 1425 343 324 231 3321 Fragments arranged by size: 3321 1425 343 324 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2210 3656 4015 4174 518 Size: 648 843 201 1446 359 159 2042 Fragments arranged by size: 2042 1446 843 648 359 201 159 MspA1I CmG'CkG Cuts at: 1606 2358 2395 2512 2584 3114 3791 4939 5184 Size: 752 37 117 72 530 677 1148 245 Cuts at: 5184 1606 Size: 2120 Fragments arranged by size: 2120 1148 752 677 530 245 117 72 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 3056 4601 1132 Size: 97 1827 1545 2229 Fragments arranged by size: 2229 1827 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 2039 2599 1108 Size: 931 560 4207 Fragments arranged by size: 4207 931 560 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2437 2437 Size: 5698 PshAI GACnn'nnGTC Cuts at: 1613 2177 1613 Size: 564 5134 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 2520 2583 2898 2967 3050 830 Size: 1690 63 315 69 83 3478 Fragments arranged by size: 3478 1690 315 83 69 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2395 2584 2395 Size: 189 5509 SacI G_AGCT'C Cuts at: 2883 2883 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SexAI A'CCwGG_T Cuts at: 2953 2953 Size: 5698 SfcI C'TryA_G Cuts at: 826 1045 2083 2516 2579 2894 2963 3046 4181 Size: 219 1038 433 63 315 69 83 1135 Cuts at: 4181 4862 5053 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1038 681 433 315 219 191 83 69 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 2141 2867 2945 1517 Size: 624 726 78 4270 Fragments arranged by size: 4270 726 624 78 StyI C'CwwG_G Cuts at: 513 1065 2004 2796 3137 513 Size: 552 939 792 341 3074 Fragments arranged by size: 3074 939 792 552 341 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI