################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp718I AspEI AvrII BbrPI BcgI BciVI BfrI BlnI BsiWI BsmI BspDI BspHI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I ClaI DraI DrdI Eam1105I EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI KspI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SapI SbfI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 2435 ACATGT A^C[GA][CT]GT Alw44I 2125 GTGCAC G^TGCAC ApaLI 2125 GTGCAC G^TGCAC Asp700I 1788 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2629 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 242 TGCGCA TGC^GCA BclI 212 TGATCA T^GATCA BsaAI 679 TACGTG [CT]AC^GT[GA] BsaBI 2163 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2242 GAGACC GGTCTC(1/5) BsmBI 1666 GAGACG CGTCTC(1/5) Bsp1407I 2438 TGTACA T^GTACA BspLU11I 2435 ACATGT A^CATGT BsrGI 2438 TGTACA T^GTACA BstAPI 378 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2454 GGTGACC G^GT[ACGT]ACC BstPI 2454 GGTGACC G^GT[ACGT]ACC CpoI 340 CGGACCG CG^G[AT]CCG DraIII 379 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1779 GAAGAG CTCTTC(1/4) EcoO65I 2454 GGTGACC G^GT[ACGT]ACC FbaI 212 TGATCA T^GATCA FspI 242 TGCGCA TGC^GCA HincII 638 GTCAAC GT[CT]^[GA]AC HindII 638 GTCAAC GT[CT]^[GA]AC Ksp632I 1779 GAAGAG CTCTTC(1/4) MamI 2163 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 578 TTAA T^TAA NcoI 2495 CCATGG C^CATGG NheI 822 GCTAGC G^CTAGC PflFI 2629 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT] RsrII 340 CGGACCG CG^G[AT]CCG ScaI 620 AGTACT AGT^ACT SfiI 18 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 2438 TGTACA T^GTACA Tru9I 578 TTAA T^TAA Tsp509I 1865 AATT ^AATT Tth111I 2629 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1788 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 772 CCTGAGG CC^T[ACGT]AGG 1965 CCTCAGG BamHI 1023 GGATCC G^GATCC 1421 GGATCC BbeI 43 GGCGCC GGCGC^C 1278 GGCGCC BbvCI 2091 CCTCAGC CCTCAGC(-5/-2) 2285 CCTCAGC BglII 1161 AGATCT A^GATCT 1550 AGATCT BsrBI 2368 CCGCTC CCGCTC(-3/-3) 1891 GAGCGG BsrFI 858 GCCGGC [GA]^CCGG[CT] 2134 GCCGGC BstXI 1379 CCAGGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2297 CCACGCCCGTGG Bsu36I 772 CCTGAGG CC^T[ACGT]AGG 1965 CCTCAGG Cfr10I 858 GCCGGC [GA]^CCGG[CT] 2134 GCCGGC Eco81I 772 CCTGAGG CC^T[ACGT]AGG 1965 CCTCAGG EcoNI 240 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2511 CCTGGAGGAGG FauI 1251 GCGGG CCCGC(4/6) 2099 GCGGG KasI 43 GGCGCC G^GCGCC 1278 GGCGCC NaeI 858 GCCGGC GCC^GGC 2134 GCCGGC NarI 43 GGCGCC GG^CGCC 1278 GGCGCC NgoMIV 858 GCCGGC G^CCGGC 2134 GCCGGC PaeR7I 817 CTCGAG C^TCGAG 839 CTCGAG PflMI 1224 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2379 CCAGCAGGTGG SexAI 380 ACCTGGT A^CC[AT]GGT 1718 ACCTGGT SfoI 43 GGCGCC GGC^GCC 1278 GGCGCC Van91I 1224 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2379 CCAGCAGGTGG XbaI 520 TCTAGA T^CTAGA 1044 TCTAGA XcmI 2070 CCAGCTCAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2294 CCACCACGCCCGTGG XhoI 817 CTCGAG C^TCGAG 839 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI Aor51HI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco47III Eco52I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII