(Linear) (Six-base) MAPSORT of: KIEE2123.seq Check: 5561 from: 1 to: 2742 KIEE2123.orf With 182 enzymes: SgfI * February 3, 2015 16:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1348 2373 2742 Size: 1348 1025 369 Fragments arranged by size: 1348 1025 369 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1510 2593 2659 2742 Size: 1510 1083 66 83 Fragments arranged by size: 1510 1083 83 66 AfeI AGC'GCT Cuts at: 0 283 1999 2329 2742 Size: 283 1716 330 413 Fragments arranged by size: 1716 413 330 283 AflIII A'CryG_T Cuts at: 0 2435 2742 Size: 2435 307 AleI CACnn'nnGTG Cuts at: 0 2302 2742 Size: 2302 440 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 463 495 2194 2226 2742 Size: 463 32 1699 32 516 Fragments arranged by size: 1699 516 463 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 468 772 1542 2742 Size: 468 304 770 1200 Fragments arranged by size: 1200 770 468 304 ApaLI G'TGCA_C Cuts at: 0 2125 2742 Size: 2125 617 AvaI C'yCGr_G Cuts at: 0 289 817 839 1345 2742 Size: 289 528 22 506 1397 Fragments arranged by size: 1397 528 506 289 22 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2382 2415 2742 Size: 2382 33 327 Fragments arranged by size: 2382 327 33 BamHI G'GATC_C Cuts at: 0 1023 1421 2742 Size: 1023 398 1321 Fragments arranged by size: 1321 1023 398 BanI G'GyrC_C Cuts at: 0 43 384 604 1215 1278 1606 1684 2742 Size: 43 341 220 611 63 328 78 1058 Fragments arranged by size: 1058 611 341 328 220 78 63 43 BanII G_rGCy'C Cuts at: 0 67 187 818 944 1237 1650 1940 2033 Size: 67 120 631 126 293 413 290 93 Cuts at: 2033 2345 2742 Size: 312 397 Fragments arranged by size: 631 413 397 312 293 290 126 120 93 67 BbeI G_GCGC'C Cuts at: 0 47 1282 2742 Size: 47 1235 1460 Fragments arranged by size: 1460 1235 47 BbsI GAAGACnn'nnnn_ Cuts at: 0 1501 1511 2589 2742 Size: 1501 10 1078 153 Fragments arranged by size: 1501 1078 153 10 BbvCI CC'TCA_GC Cuts at: 0 2092 2286 2742 Size: 2092 194 456 Fragments arranged by size: 2092 456 194 BclI T'GATC_A Cuts at: 0 212 2742 Size: 212 2530 BglI GCCn_nnn'nGGC Cuts at: 0 25 511 1092 2742 Size: 25 486 581 1650 Fragments arranged by size: 1650 581 486 25 BglII A'GATC_T Cuts at: 0 1161 1550 2742 Size: 1161 389 1192 Fragments arranged by size: 1192 1161 389 BlpI GC'TnA_GC Cuts at: 0 1897 2119 2259 2742 Size: 1897 222 140 483 Fragments arranged by size: 1897 483 222 140 Bme1580I G_kGCm'C Cuts at: 0 607 723 1220 1609 1687 1705 2129 2742 Size: 607 116 497 389 78 18 424 613 Fragments arranged by size: 613 607 497 424 389 116 78 18 BmgBI CAC'GTC Cuts at: 0 1499 2154 2421 2742 Size: 1499 655 267 321 Fragments arranged by size: 1499 655 321 267 BmrI ACTGGGnnnn_n' Cuts at: 0 847 1299 2742 Size: 847 452 1443 Fragments arranged by size: 1443 847 452 BmtI G_CTAG'C Cuts at: 0 826 2742 Size: 826 1916 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1572 1626 2742 Size: 1572 54 1116 Fragments arranged by size: 1572 1116 54 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 382 730 763 794 848 884 908 Size: 124 258 348 33 31 54 36 24 Cuts at: 908 928 938 968 998 1046 1072 1142 1648 Size: 20 10 30 30 48 26 70 506 Cuts at: 1648 1954 2022 2250 2337 2533 2742 Size: 306 68 228 87 196 209 Fragments arranged by size: 506 348 306 258 228 209 196 124 87 70 68 54 48 36 33 31 30 30 26 24 20 10 Bpu10I CC'TnA_GC Cuts at: 0 2074 2092 2286 2742 Size: 2074 18 194 456 Fragments arranged by size: 2074 456 194 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 1411 2704 2742 Size: 646 765 1293 38 Fragments arranged by size: 1293 765 646 38 BsaI GGTCTCn'nnnn_ Cuts at: 0 2236 2742 Size: 2236 506 BsaAI yAC'GTr Cuts at: 0 681 2742 Size: 681 2061 BsaBI GATnn'nnATC Cuts at: 0 2167 2742 Size: 2167 575 BsaHI Gr'CG_yC Cuts at: 0 44 92 113 503 1279 2742 Size: 44 48 21 390 776 1463 Fragments arranged by size: 1463 776 390 48 44 21 BsaWI w'CCGG_w Cuts at: 0 645 936 1526 2564 2742 Size: 645 291 590 1038 178 Fragments arranged by size: 1038 645 590 291 178 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 220 250 882 912 2341 2371 2654 2684 Size: 220 30 632 30 1429 30 283 30 Cuts at: 2684 2742 Size: 58 Fragments arranged by size: 1429 632 283 220 58 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 220 507 582 1144 1797 2130 2173 2274 Size: 220 287 75 562 653 333 43 101 Cuts at: 2274 2530 2533 2536 2742 Size: 256 3 3 206 Fragments arranged by size: 653 562 333 287 256 220 206 101 75 43 3 3 BseYI C'CCAG_C Cuts at: 0 737 847 1247 2020 2742 Size: 737 110 400 773 722 Fragments arranged by size: 773 737 722 400 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 124 241 271 703 2742 Size: 25 99 117 30 432 2039 Fragments arranged by size: 2039 432 117 99 30 25 BsiEI CG_ry'CG Cuts at: 0 29 1660 2010 2135 2742 Size: 29 1631 350 125 607 Fragments arranged by size: 1631 607 350 125 29 BsiHKAI G_wGCw'C Cuts at: 0 187 380 749 818 846 944 1274 1394 Size: 187 193 369 69 28 98 330 120 Cuts at: 1394 2033 2129 2742 Size: 639 96 613 Fragments arranged by size: 639 613 369 330 193 187 120 98 96 69 28 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1660 2742 Size: 1660 1082 Bsp1286I G_dGCh'C Cuts at: 0 67 187 380 607 723 749 818 846 Size: 67 120 193 227 116 26 69 28 Cuts at: 846 944 1220 1237 1274 1394 1609 1650 1687 Size: 98 276 17 37 120 215 41 37 Cuts at: 1687 1705 1940 2033 2129 2345 2742 Size: 18 235 93 96 216 397 Fragments arranged by size: 397 276 235 227 216 215 193 120 120 116 98 96 93 69 67 41 37 37 28 26 18 17 BspEI T'CCGG_A Cuts at: 0 645 936 1526 2742 Size: 645 291 590 1216 Fragments arranged by size: 1216 645 590 291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 176 482 1348 2373 2742 Size: 176 306 866 1025 369 Fragments arranged by size: 1025 866 369 306 176 BsrBI CCG'CTC Cuts at: 0 1893 2370 2742 Size: 1893 477 372 Fragments arranged by size: 1893 477 372 BsrFI r'CCGG_y Cuts at: 0 858 2134 2742 Size: 858 1276 608 Fragments arranged by size: 1276 858 608 BsrGI T'GTAC_A Cuts at: 0 2438 2742 Size: 2438 304 BstAPI GCAn_nnn'nTGC Cuts at: 0 384 2742 Size: 384 2358 BstEII G'GTnAC_C Cuts at: 0 2454 2742 Size: 2454 288 BstXI CCAn_nnnn'nTGG Cuts at: 0 1386 2304 2742 Size: 1386 918 438 Fragments arranged by size: 1386 918 438 BstYI r'GATC_y Cuts at: 0 1023 1161 1421 1529 1550 2576 2742 Size: 1023 138 260 108 21 1026 166 Fragments arranged by size: 1026 1023 260 166 138 108 21 Bsu36I CC'TnA_GG Cuts at: 0 773 1966 2742 Size: 773 1193 776 Fragments arranged by size: 1193 776 773 BtgI C'CryG_G Cuts at: 0 261 1886 2303 2495 2742 Size: 261 1625 417 192 247 Fragments arranged by size: 1625 417 261 247 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1833 2091 2742 Size: 1833 258 651 Fragments arranged by size: 1833 651 258 BtsI GCAGTG_nn' Cuts at: 0 1224 1733 2742 Size: 1224 509 1009 Fragments arranged by size: 1224 1009 509 DraIII CAC_nnn'GTG Cuts at: 0 384 2742 Size: 384 2358 EaeI y'GGCC_r Cuts at: 0 26 1319 1406 1657 2132 2196 2742 Size: 26 1293 87 251 475 64 546 Fragments arranged by size: 1293 546 475 251 87 64 26 EagI C'GGCC_G Cuts at: 0 26 1657 2132 2742 Size: 26 1631 475 610 Fragments arranged by size: 1631 610 475 26 EarI CTCTTCn'nnn_ Cuts at: 0 1774 2742 Size: 1774 968 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 87 2742 Size: 87 2655 EcoICRI GAG'CTC Cuts at: 0 185 816 942 2031 2742 Size: 185 631 126 1089 711 Fragments arranged by size: 1089 711 631 185 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 382 730 763 794 848 884 908 Size: 124 258 348 33 31 54 36 24 Cuts at: 908 928 938 968 998 1046 1072 1142 1510 Size: 20 10 30 30 48 26 70 368 Cuts at: 1510 1648 1954 2022 2250 2337 2533 2593 2659 Size: 138 306 68 228 87 196 60 66 Cuts at: 2659 2742 Size: 83 Fragments arranged by size: 368 348 306 258 228 196 138 124 87 83 70 68 66 60 54 48 36 33 31 30 30 26 24 20 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 244 2515 2742 Size: 244 2271 227 Fragments arranged by size: 2271 244 227 EcoO109I rG'GnC_Cy Cuts at: 0 465 1871 2016 2742 Size: 465 1406 145 726 Fragments arranged by size: 1406 726 465 145 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2667 2742 Size: 2667 75 FspI TGC'GCA Cuts at: 0 244 2742 Size: 244 2498 HaeII r_GCGC'y Cuts at: 0 47 285 717 991 1069 1282 2001 2331 Size: 47 238 432 274 78 213 719 330 Cuts at: 2331 2742 Size: 411 Fragments arranged by size: 719 432 411 330 274 238 213 78 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 289 321 607 639 766 798 1927 1959 Size: 289 32 286 32 127 32 1129 32 Cuts at: 1959 2654 2686 2742 Size: 695 32 56 Fragments arranged by size: 1129 695 289 286 127 56 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 640 2742 Size: 640 2102 KasI G'GCGC_C Cuts at: 0 43 1278 2742 Size: 43 1235 1464 Fragments arranged by size: 1464 1235 43 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 572 913 2174 2742 Size: 572 341 1261 568 Fragments arranged by size: 1261 572 568 341 MscI TGG'CCA Cuts at: 0 1321 1408 2198 2742 Size: 1321 87 790 544 Fragments arranged by size: 1321 790 544 87 MslI CAynn'nnrTG Cuts at: 0 1738 2302 2452 2742 Size: 1738 564 150 290 Fragments arranged by size: 1738 564 290 150 MspA1I CmG'CkG Cuts at: 0 54 125 255 669 741 964 1251 2099 Size: 54 71 130 414 72 223 287 848 Cuts at: 2099 2742 Size: 643 Fragments arranged by size: 848 643 414 287 223 130 72 71 54 NaeI GCC'GGC Cuts at: 0 860 2136 2742 Size: 860 1276 606 Fragments arranged by size: 1276 860 606 NarI GG'CG_CC Cuts at: 0 44 1279 2742 Size: 44 1235 1463 Fragments arranged by size: 1463 1235 44 NcoI C'CATG_G Cuts at: 0 2495 2742 Size: 2495 247 NgoMIV G'CCGG_C Cuts at: 0 858 2134 2742 Size: 858 1276 608 Fragments arranged by size: 1276 858 608 NheI G'CTAG_C Cuts at: 0 822 2742 Size: 822 1920 NspI r_CATG'y Cuts at: 0 15 696 2439 2742 Size: 15 681 1743 303 Fragments arranged by size: 1743 681 303 15 PciI A'CATG_T Cuts at: 0 2435 2742 Size: 2435 307 PflMI CCAn_nnn'nTGG Cuts at: 0 1230 2385 2742 Size: 1230 1155 357 Fragments arranged by size: 1230 1155 357 PfoI T'CCnGG_A Cuts at: 0 467 2180 2742 Size: 467 1713 562 Fragments arranged by size: 1713 562 467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1558 1590 2742 Size: 1558 32 1152 Fragments arranged by size: 1558 1152 32 PpuMI rG'GwC_Cy Cuts at: 0 465 2742 Size: 465 2277 PspXI vC'TCGA_Gb Cuts at: 0 817 839 2742 Size: 817 22 1903 Fragments arranged by size: 1903 817 22 PstI C_TGCA'G Cuts at: 0 1469 1826 2549 2742 Size: 1469 357 723 193 Fragments arranged by size: 1469 723 357 193 PvuII CAG'CTG Cuts at: 0 125 255 669 741 964 2742 Size: 125 130 414 72 223 1778 Fragments arranged by size: 1778 414 223 130 125 72 RsrII CG'GwC_CG Cuts at: 0 341 2742 Size: 341 2401 SacI G_AGCT'C Cuts at: 0 187 818 944 2033 2742 Size: 187 631 126 1089 709 Fragments arranged by size: 1089 709 631 187 126 ScaI AGT'ACT Cuts at: 0 622 2742 Size: 622 2120 SexAI A'CCwGG_T Cuts at: 0 380 1718 2742 Size: 380 1338 1024 Fragments arranged by size: 1338 1024 380 SfcI C'TryA_G Cuts at: 0 438 1465 1822 1842 2545 2742 Size: 438 1027 357 20 703 197 Fragments arranged by size: 1027 703 438 357 197 20 SfiI GGCCn_nnn'nGGCC Cuts at: 0 25 2742 Size: 25 2717 SfoI GGC'GCC Cuts at: 0 45 1280 2742 Size: 45 1235 1462 Fragments arranged by size: 1462 1235 45 SmlI C'TyrA_G Cuts at: 0 661 817 839 1426 2719 2742 Size: 661 156 22 587 1293 23 Fragments arranged by size: 1293 661 587 156 23 22 StyI C'CwwG_G Cuts at: 0 2495 2657 2729 2742 Size: 2495 162 72 13 Fragments arranged by size: 2495 162 72 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 472 861 1318 2335 2742 Size: 472 389 457 1017 407 Fragments arranged by size: 1017 472 457 407 389 TatI w'GTAC_w Cuts at: 0 620 2438 2742 Size: 620 1818 304 Fragments arranged by size: 1818 620 304 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2044 2076 2742 Size: 2044 32 666 Fragments arranged by size: 2044 666 32 Tth111I GACn'n_nGTC Cuts at: 0 2632 2742 Size: 2632 110 XbaI T'CTAG_A Cuts at: 0 520 1044 2742 Size: 520 524 1698 Fragments arranged by size: 1698 524 520 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2077 2301 2742 Size: 2077 224 441 Fragments arranged by size: 2077 441 224 XhoI C'TCGA_G Cuts at: 0 817 839 2742 Size: 817 22 1903 Fragments arranged by size: 1903 817 22 XmnI GAAnn'nnTTC Cuts at: 0 1792 2742 Size: 1792 950 Enzymes that do cut: AarI AcuI AfeI AflIII AleI AloI AlwNI ApaLI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI PfoI PpiI PpuMI PspXI PstI PvuII RsrII SacI ScaI SexAI SfcI SfiI SfoI SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AlfI ApaI ApoI AscI AseI AsiSI AvrII BcgI BciVI BfrBI BsiWI BsmI BspHI BsrDI BssHII BssSI BstBI BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI NotI NruI NsiI PacI PasI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI SacII SalI SanDI SapI SbfI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XmaI ZraI