(Circular) (Six-base) MAPSORT of: pFN21AB7666.seq Check: 9463 from: 1 to: 5818 pFN21AB7666 5818 bp With 182 enzymes: SgfI * January 9, 2015 16:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3881 5265 5533 1632 Size: 2249 1384 268 1917 Fragments arranged by size: 2249 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 2379 4717 5694 1203 Size: 1176 2338 977 1327 Fragments arranged by size: 2338 1327 1176 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 2164 4547 2164 Size: 2383 3435 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AlwNI CAG_nnn'CTG Cuts at: 2576 3561 5133 2576 Size: 985 1572 3261 Fragments arranged by size: 3261 1572 985 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 3127 3353 3505 5614 1493 Size: 1634 226 152 2109 1697 Fragments arranged by size: 2109 1697 1634 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2329 2410 3143 1870 Size: 73 386 81 733 4545 Fragments arranged by size: 4545 733 386 81 73 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3139 4594 618 Size: 325 217 627 1352 1455 1842 Fragments arranged by size: 1842 1455 1352 627 325 217 BanII G_rGCy'C Cuts at: 1391 2250 3137 1391 Size: 859 887 4072 Fragments arranged by size: 4072 887 859 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 928 Size: 971 261 4586 Fragments arranged by size: 4586 971 261 BbvCI CC'TCA_GC Cuts at: 2772 2772 Size: 5818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4009 4043 1903 Size: 34 50 34 1988 34 3678 Fragments arranged by size: 3678 1988 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4429 136 Size: 107 122 71 631 605 2757 1525 Fragments arranged by size: 2757 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2112 5813 2112 Size: 3701 2117 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 2433 3879 5035 1790 Size: 643 1446 1156 2573 Fragments arranged by size: 2573 1446 1156 643 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5818 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3549 4507 442 Size: 416 53 83 399 24 2132 958 1753 Fragments arranged by size: 2132 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2487 2553 4478 1239 Size: 1248 66 1925 2579 Fragments arranged by size: 2579 1925 1248 66 Bpu10I CC'TnA_GC Cuts at: 2772 2772 Size: 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4481 882 Size: 617 2982 2219 Fragments arranged by size: 2982 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 3609 493 Size: 711 1560 845 2702 Fragments arranged by size: 2702 1560 845 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3625 4007 4642 275 Size: 53 83 186 936 2092 382 635 1451 Fragments arranged by size: 2092 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2018 4251 4649 4923 5070 5481 2018 Size: 2233 398 274 147 411 2355 Fragments arranged by size: 2355 2233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5593 Fragments arranged by size: 5593 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2037 1186 Size: 851 4967 BseYI C'CCAG_C Cuts at: 5021 5517 5021 Size: 496 5322 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 2919 1163 Size: 573 723 460 4062 Fragments arranged by size: 4062 723 573 460 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3268 4029 5057 1540 Size: 275 186 1267 761 1028 2301 Fragments arranged by size: 2301 1267 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2496 3879 3964 5035 2496 Size: 1383 85 1071 3279 Fragments arranged by size: 3279 1383 1071 85 BsmI GAATG_Cn' Cuts at: 2836 3329 3422 2836 Size: 493 93 5232 Fragments arranged by size: 5232 493 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2433 2496 3137 3879 3964 5035 Size: 399 460 183 63 641 742 85 1071 Cuts at: 5035 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 641 460 399 183 85 63 BspEI T'CCGG_A Cuts at: 2018 5481 2018 Size: 3463 2355 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3173 844 Size: 2329 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3705 1394 Size: 429 1882 3507 Fragments arranged by size: 3507 1882 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4462 4649 1700 Size: 110 2652 187 2869 Fragments arranged by size: 2869 2652 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5818 BssSI C'ACGA_G Cuts at: 2213 3878 4890 2213 Size: 1665 1012 3141 Fragments arranged by size: 3141 1665 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstXI CCAn_nnnn'nTGG Cuts at: 2687 2918 2687 Size: 231 5587 BstYI r'GATC_y Cuts at: 1972 2112 2390 3148 3515 3900 3917 5369 5455 Size: 140 278 758 367 385 17 1452 86 Cuts at: 5455 5467 5813 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 758 385 367 346 278 140 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2718 2718 Size: 5818 BtgI C'CryG_G Cuts at: 513 1065 2061 2433 513 Size: 552 996 372 3898 Fragments arranged by size: 3898 996 552 372 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DraIII CAC_nnn'GTG Cuts at: 2218 2218 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2220 3265 Size: 54 1425 50 275 18 369 21 1045 Cuts at: 3265 4154 5783 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1045 889 369 275 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3265 1537 Size: 275 1453 4090 Fragments arranged by size: 4090 1453 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2943 3044 3050 3748 1353 Size: 780 810 101 6 698 3423 Fragments arranged by size: 3423 810 780 698 101 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2553 3881 4478 5265 5533 1239 Size: 393 855 66 1328 597 787 268 1524 Fragments arranged by size: 1524 1328 855 787 597 393 268 66 EcoO109I rG'GnC_Cy Cuts at: 2044 2318 2044 Size: 274 5544 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 EcoRV GAT'ATC Cuts at: 2581 2581 Size: 5818 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2188 Size: 5818 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 4965 1678 Size: 3287 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2116 2148 2150 2182 2525 Size: 32 135 32 544 32 2 32 343 Cuts at: 2525 2557 2650 2682 2853 2885 4459 4491 4533 Size: 32 93 32 171 32 1574 32 42 Cuts at: 4533 4565 1373 Size: 32 2626 Fragments arranged by size: 2626 1574 544 343 171 135 93 42 32 32 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 2539 3162 3408 669 Size: 988 281 601 623 246 3079 Fragments arranged by size: 3079 988 623 601 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2596 4932 5116 1305 Size: 1291 2336 184 2007 Fragments arranged by size: 2336 2007 1291 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 10 Size: 54 1425 343 390 3606 Fragments arranged by size: 3606 1425 390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2277 2295 2351 3776 4135 4294 518 Size: 648 1111 18 56 1425 359 159 2042 Fragments arranged by size: 2042 1425 1111 648 359 159 56 18 MspA1I CmG'CkG Cuts at: 1606 3107 3234 3911 5059 5304 1606 Size: 1501 127 677 1148 245 2120 Fragments arranged by size: 2120 1501 1148 677 245 127 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5266 NdeI CA'TA_TG Cuts at: 387 2655 2997 387 Size: 2268 342 3208 Fragments arranged by size: 3208 2268 342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 2383 2557 2936 3176 4721 1132 Size: 97 1154 174 379 240 1545 2229 Fragments arranged by size: 2229 1545 1154 379 240 174 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 2379 4717 2379 Size: 2338 3480 PflMI CCAn_nnn'nTGG Cuts at: 1108 2636 1108 Size: 1528 4290 PfoI T'CCnGG_A Cuts at: 2503 2503 Size: 5818 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PmlI CAC'GTG Cuts at: 2764 2764 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2044 2318 2044 Size: 274 5544 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PspXI vC'TCGA_Gb Cuts at: 2329 2329 Size: 5818 PstI C_TGCA'G Cuts at: 830 3170 830 Size: 2340 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 PvuII CAG'CTG Cuts at: 3107 3107 Size: 5818 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SanDI GG'GwC_CC Cuts at: 2044 2318 2044 Size: 274 5544 SapI GCTCTTCn'nnn_ Cuts at: 2943 2943 Size: 5818 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 3068 3166 4301 4982 5173 826 Size: 219 2023 98 1135 681 191 1471 Fragments arranged by size: 2023 1471 1135 681 219 191 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 3145 1872 Size: 1273 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2329 3922 4823 5085 820 Size: 197 926 386 1593 901 262 1553 Fragments arranged by size: 1593 1553 926 901 386 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 2936 3176 1132 Size: 1804 240 3774 Fragments arranged by size: 3774 1804 240 SspI AAT'ATT Cuts at: 5 52 2963 3742 5 Size: 47 2911 779 2081 Fragments arranged by size: 2911 2081 779 47 StuI AGG'CCT Cuts at: 1517 2304 1517 Size: 787 5031 StyI C'CwwG_G Cuts at: 513 1065 2172 3257 513 Size: 552 1107 1085 3074 Fragments arranged by size: 3074 1107 1085 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2419 3845 3862 4015 4200 4645 325 Size: 1255 839 1426 17 153 185 445 1498 Fragments arranged by size: 1498 1426 1255 839 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2882 4064 5776 Size: 274 80 33 51 1443 905 1182 1712 Cuts at: 5776 96 Size: 138 Fragments arranged by size: 1712 1443 1182 905 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2202 2234 5778 5810 2202 Size: 32 3544 32 2210 Fragments arranged by size: 3544 2210 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2637 2637 Size: 5818 XhoI C'TCGA_G Cuts at: 1943 2329 1943 Size: 386 5432 XmaI C'CCGG_G Cuts at: 1870 3143 1870 Size: 1273 4545 XmnI GAAnn'nnTTC Cuts at: 3947 3947 Size: 5818 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI