################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AviII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrBI BssHII BstAPI BstEII BstPI BstUI CfoI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 1338 GGTACC G^GTACC AccI 240 GTATAC GT^[AC][GT]AC AciI 2217 CCGC CCGC(-3/-1) Asp718I 1338 GGTACC G^GTACC AspI 2197 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 24 CCTAGG C^CTAGG BalI 854 TGGCCA TGG^CCA BbsI 898 GTCTTC GAAGAC(2/6) BlnI 24 CCTAGG C^CTAGG BlpI 156 GCTGAGC GC^T[ACGT]AGC Bpu1102I 156 GCTGAGC GC^T[ACGT]AGC BpuAI 898 GTCTTC GAAGAC(2/6) BseRI 1380 CTCCTC GAGGAG(10/8) BsgI 2119 GTGCAG GTGCAG(16/14) BsmI 2124 GAATGC GAATGC(1/-1) BspHI 1961 TCATGA T^CATGA BsrFI 43 GCCGGT [GA]^CCGG[CT] BssSI 914 CACGAG CACGAG(-5/-1) Bst1107I 240 GTATAC GTA^TAC BstBI 1059 TTCGAA TT^CGAA BstXI 2466 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 240 GTATAC GTA^TAC Cac8I 847 GCTGGC GC[ACGT]^[ACGT]GC CelII 156 GCTGAGC GC^T[ACGT]AGC Cfr10I 43 GCCGGT [GA]^CCGG[CT] DraIII 1709 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1874 CCACGG C^C[GA][CT]GG EcoNI 111 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1219 GATATC GAT^ATC HphI 113 TCACC GGTGA(8/7) KpnI 1338 GGTACC GGTAC^C MluNI 854 TGGCCA TGG^CCA MscI 854 TGGCCA TGG^CCA NspV 1059 TTCGAA TT^CGAA PaeR7I 568 CTCGAG C^TCGAG PflFI 2197 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1961 TCATGA T^CATGA SfuI 1059 TTCGAA TT^CGAA SpeI 1242 ACTAGT A^CTAGT SspI 543 AATATT AAT^ATT Tth111I 2197 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1311 TCTAGA T^CTAGA XhoI 568 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 1078 CCTAAGG CC^T[ACGT]AGG 2455 CCTGAGG Asp700I 59 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 660 GAACCCATTC AvaI 229 CTCGGG C^[CT]CG[GA]G 568 CTCGAG BcnI 800 CCGGG CC^[GC]GG 1989 CCGGG BsaI 5 GAGACC GGTCTC(1/5) 388 GGTCTC BsoBI 229 CTCGGG C^[CT]CG[GA]G 568 CTCGAG Bsp1407I 2069 TGTACA T^GTACA 2427 TGTACA BsrGI 2069 TGTACA T^GTACA 2427 TGTACA Bsu36I 1078 CCTAAGG CC^T[ACGT]AGG 2455 CCTGAGG DraI 429 TTTAAA TTT^AAA 1362 TTTAAA EarI 1861 CTCTTC CTCTTC(1/4) 1891 GAAGAG Eco81I 1078 CCTAAGG CC^T[ACGT]AGG 2455 CCTGAGG EcoRI 59 GAATTC G^AATTC 507 GAATTC Ksp632I 1861 CTCTTC CTCTTC(1/4) 1891 GAAGAG MspA1I 211 CAGCTG C[AC]G^C[GT]G 217 CAGCTG NciI 800 CCGGG CC^[GC]GG 1989 CCGGG PflMI 786 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2022 CCAGAGACTGG PstI 214 CTGCAG CTGCA^G 1814 CTGCAG PvuII 211 CAGCTG CAG^CTG 217 CAGCTG SspBI 2069 TGTACA T^GTACA 2427 TGTACA StuI 266 AGGCCT AGG^CCT 761 AGGCCT TaqI 569 TCGA T^CGA 1060 TCGA TthHB8I 569 TCGA T^CGA 1060 TCGA Van91I 786 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2022 CCAGAGACTGG XmnI 59 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 660 GAACCCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII