(Circular) (Six-base) MAPSORT of: pFN21AE2134.seq Check: 3768 from: 1 to: 7279 pFN21AE2134 7279 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4326 4326 Size: 7279 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5089 278 Size: 53 83 186 936 3553 2468 Fragments arranged by size: 3553 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2246 4622 1937 Size: 309 2376 4594 Fragments arranged by size: 4594 2376 309 Acc65I G'GTAC_C Cuts at: 3343 4600 3343 Size: 1257 6022 AclI AA'CG_TT Cuts at: 1563 5406 5779 1563 Size: 3843 373 3063 Fragments arranged by size: 3843 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2260 3396 4084 4303 5342 6726 6994 1632 Size: 628 1136 688 219 1039 1384 268 1917 Fragments arranged by size: 1917 1384 1136 1039 688 628 268 219 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7082 AflIII A'CryG_T Cuts at: 1203 6178 7155 1203 Size: 4975 977 1327 Fragments arranged by size: 4975 1327 977 AgeI A'CCGG_T Cuts at: 6110 6110 Size: 7279 AhdI GACnn_n'nnGTC Cuts at: 6008 6008 Size: 7279 AleI CACnn'nnGTG Cuts at: 1166 4121 1166 Size: 2955 4324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2358 1184 Size: 1174 6105 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3423 3455 3423 Size: 32 7247 AlwNI CAG_nnn'CTG Cuts at: 2189 2227 2356 4033 4067 5022 6594 2189 Size: 38 129 1677 34 955 1572 2874 Fragments arranged by size: 2874 1677 1572 955 129 38 34 ApaLI G'TGCA_C Cuts at: 5336 6492 5336 Size: 1156 6123 ApoI r'AATT_y Cuts at: 1493 2064 2431 2512 3067 3146 4588 4814 4966 Size: 571 367 81 555 79 1442 226 152 Cuts at: 4966 7075 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1442 571 555 367 226 152 81 79 AseI AT'TA_AT Cuts at: 160 1032 5833 160 Size: 872 4801 1606 Fragments arranged by size: 4801 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7279 AvaI C'yCGr_G Cuts at: 1870 1943 2234 2573 4604 1870 Size: 73 291 339 2031 4545 Fragments arranged by size: 4545 2031 339 291 73 AvrII C'CTAG_G Cuts at: 2029 2029 Size: 7279 BamHI G'GATC_C Cuts at: 4609 4976 4609 Size: 367 6912 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2801 3343 4305 4600 6055 Size: 325 217 627 1014 542 962 295 1455 Cuts at: 6055 618 Size: 1842 Fragments arranged by size: 1842 1455 1014 962 627 542 325 295 217 BanII G_rGCy'C Cuts at: 1391 2235 2574 2580 3483 4598 1391 Size: 844 339 6 903 1115 4072 Fragments arranged by size: 4072 1115 903 844 339 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2896 928 Size: 971 997 5311 Fragments arranged by size: 5311 997 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5470 5504 1903 Size: 34 3533 34 3678 Fragments arranged by size: 3678 3533 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5171 6381 5171 Size: 1210 6069 BclI T'GATC_A Cuts at: 1548 1548 Size: 7279 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5890 136 Size: 107 122 71 631 605 4218 1525 Fragments arranged by size: 4218 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7274 7274 Size: 7279 BlpI GC'TnA_GC Cuts at: 2162 4696 2162 Size: 2534 4745 Bme1580I G_kGCm'C Cuts at: 1790 4061 5340 6496 1790 Size: 2271 1279 1156 2573 Fragments arranged by size: 2573 2271 1279 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7279 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2659 4387 5010 Size: 416 53 83 399 24 1242 1728 623 Cuts at: 5010 5968 442 Size: 958 1753 Fragments arranged by size: 1753 1728 1242 958 623 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7279 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2057 2057 Size: 7279 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2708 3338 4454 5939 1239 Size: 1010 459 630 1116 1485 2579 Fragments arranged by size: 2579 1485 1116 1010 630 459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 3253 5404 6269 6567 2921 Size: 332 2151 865 298 3633 Fragments arranged by size: 3633 2151 865 332 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2004 2399 5942 882 Size: 617 505 395 3543 2219 Fragments arranged by size: 3543 2219 617 505 395 BsaAI yAC'GTr Cuts at: 493 1204 5070 493 Size: 711 3866 2702 Fragments arranged by size: 3866 2702 711 BsaBI GATnn'nnATC Cuts at: 7273 7273 Size: 7279 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5086 5468 6103 275 Size: 53 83 186 936 3553 382 635 1451 Fragments arranged by size: 3553 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5712 6110 6384 6531 6942 5712 Size: 398 274 147 411 6049 Fragments arranged by size: 6049 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3336 3366 1178 Size: 30 165 30 1933 30 5091 Fragments arranged by size: 5091 1933 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3376 1186 Size: 2190 5089 BseYI C'CCAG_C Cuts at: 3422 3809 6482 6978 3422 Size: 387 2673 496 3723 Fragments arranged by size: 3723 2673 496 387 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4145 1163 Size: 573 2409 4297 Fragments arranged by size: 4297 2409 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4729 5490 6518 1540 Size: 275 186 2728 761 1028 2301 Fragments arranged by size: 2728 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2235 2574 3483 4363 5340 5425 6496 2043 Size: 192 339 909 880 977 85 1071 2826 Fragments arranged by size: 2826 1071 977 909 880 339 192 85 BsmI GAATG_Cn' Cuts at: 4135 4790 4883 4135 Size: 655 93 6531 Fragments arranged by size: 6531 655 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2235 2574 2580 3483 4061 4363 Size: 399 253 192 339 6 903 578 302 Cuts at: 4363 4598 5340 5425 6496 1391 Size: 235 742 85 1071 2174 Fragments arranged by size: 2174 1071 903 742 578 399 339 302 253 235 192 85 6 BspEI T'CCGG_A Cuts at: 6942 6942 Size: 7279 BspHI T'CATG_A Cuts at: 3966 5168 6898 3966 Size: 1202 1730 4347 Fragments arranged by size: 4347 1730 1202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2292 3652 4326 4634 844 Size: 1448 1360 674 308 3489 Fragments arranged by size: 3489 1448 1360 674 308 BsrBI CCG'CTC Cuts at: 1394 1823 5166 1394 Size: 429 3343 3507 Fragments arranged by size: 3507 3343 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2752 3117 4224 5774 5948 65 Size: 1183 1504 365 1107 1550 174 1396 Fragments arranged by size: 1550 1504 1396 1183 1107 365 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 5923 6110 1700 Size: 110 238 3875 187 2869 Fragments arranged by size: 3875 2869 238 187 110 BsrGI T'GTAC_A Cuts at: 96 4074 4432 96 Size: 3978 358 2943 Fragments arranged by size: 3978 2943 358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7279 BssSI C'ACGA_G Cuts at: 2919 5339 6351 2919 Size: 2420 1012 3847 Fragments arranged by size: 3847 2420 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7279 BstBI TT'CG_AA Cuts at: 3065 6083 3065 Size: 3018 4261 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7279 BstXI CCAn_nnnn'nTGG Cuts at: 4478 4478 Size: 7279 BstYI r'GATC_y Cuts at: 1972 2455 2830 2884 3172 3657 4465 4609 4976 Size: 483 375 54 288 485 808 144 367 Cuts at: 4976 5361 5378 6830 6916 6928 7274 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 808 485 483 385 375 367 346 288 144 86 54 17 12 BstZ17I GTA'TAC Cuts at: 2247 2247 Size: 7279 Bsu36I CC'TnA_GG Cuts at: 3084 4461 3084 Size: 1377 5902 BtgI C'CryG_G Cuts at: 513 1065 2004 3879 513 Size: 552 939 1875 3913 Fragments arranged by size: 3913 1875 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6540 Fragments arranged by size: 6540 683 56 BtsI GCAGTG_nn' Cuts at: 2187 4795 5589 5609 2187 Size: 2608 794 20 3857 Fragments arranged by size: 3857 2608 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7279 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7244 DraI TTT'AAA Cuts at: 2436 3369 4583 2436 Size: 933 1214 5132 Fragments arranged by size: 5132 1214 933 DraIII CAC_nnn'GTG Cuts at: 3719 3719 Size: 7279 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6286 809 Size: 507 4970 1802 Fragments arranged by size: 4970 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2086 2859 Size: 54 1425 50 275 18 216 40 773 Cuts at: 2859 4726 5615 7244 8 Size: 1867 889 1629 43 Fragments arranged by size: 1867 1629 1425 889 773 275 216 54 50 43 40 18 EagI C'GGCC_G Cuts at: 1537 1812 4726 1537 Size: 275 2914 4090 Fragments arranged by size: 4090 2914 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3872 3891 5209 1353 Size: 2519 19 1318 3423 Fragments arranged by size: 3423 2519 1318 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5866 6240 6386 387 Size: 1064 138 4277 374 146 1280 Fragments arranged by size: 4277 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2233 2572 3481 2233 Size: 339 909 6031 Fragments arranged by size: 6031 909 339 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2260 2708 3338 3396 4084 4303 Size: 393 617 11 448 630 58 688 219 Cuts at: 4303 4454 5342 5939 6726 6994 1239 Size: 151 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 688 630 617 597 448 393 268 219 151 58 11 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2120 Size: 7279 EcoO109I rG'GnC_Cy Cuts at: 2125 2134 4383 2125 Size: 9 2249 5021 Fragments arranged by size: 5021 2249 9 EcoRI G'AATT_C Cuts at: 2064 2512 4588 4966 2064 Size: 448 2076 378 4377 Fragments arranged by size: 4377 2076 448 378 EcoRV GAT'ATC Cuts at: 3226 3226 Size: 7279 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2290 2344 2290 Size: 54 7225 FspI TGC'GCA Cuts at: 5785 5785 Size: 7279 HaeII r_GCGC'y Cuts at: 1678 6426 1678 Size: 4748 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 3157 3189 3585 Size: 32 135 32 950 32 603 32 396 Cuts at: 3585 3617 3836 3868 4191 4223 4255 4287 4396 Size: 32 219 32 323 32 32 32 109 Cuts at: 4396 4428 5920 5952 5994 6026 1373 Size: 32 1492 32 42 32 2626 Fragments arranged by size: 2626 1492 950 603 396 323 219 135 109 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4623 4869 669 Size: 988 281 2685 246 3079 Fragments arranged by size: 3079 2685 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7279 HpaI GTT'AAC Cuts at: 4869 4869 Size: 7279 KpnI G_GTAC'C Cuts at: 3347 4604 3347 Size: 1257 6022 MfeI C'AATT_G Cuts at: 4878 4878 Size: 7279 MluI A'CGCG_T Cuts at: 7155 7155 Size: 7279 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 3633 6393 6577 1305 Size: 1260 1068 2760 184 2007 Fragments arranged by size: 2760 2007 1260 1068 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2861 10 Size: 54 1425 343 1029 4428 Fragments arranged by size: 4428 1425 1029 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2340 3795 4121 4370 4373 5237 5596 Size: 648 1174 1455 326 249 3 864 359 Cuts at: 5596 5755 518 Size: 159 2042 Fragments arranged by size: 2042 1455 1174 864 648 359 326 249 159 3 MspA1I CmG'CkG Cuts at: 1606 2218 2224 4695 5372 6520 6765 1606 Size: 612 6 2471 677 1148 245 2120 Fragments arranged by size: 2471 2120 1148 677 612 245 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5788 Fragments arranged by size: 5788 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7279 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7279 NotI GC'GGCC_GC Cuts at: 4726 4726 Size: 7279 NspI r_CATG'y Cuts at: 1132 1229 4637 6182 1132 Size: 97 3408 1545 2229 Fragments arranged by size: 3408 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4054 4330 1348 Size: 2706 276 4297 Fragments arranged by size: 4297 2706 276 PciI A'CATG_T Cuts at: 6178 6178 Size: 7279 PflMI CCAn_nnn'nTGG Cuts at: 1108 2797 4033 1108 Size: 1689 1236 4354 Fragments arranged by size: 4354 1689 1236 PfoI T'CCnGG_A Cuts at: 3150 3660 3671 4236 4470 3150 Size: 510 11 565 234 5959 Fragments arranged by size: 5959 565 510 234 11 PmeI GTTT'AAAC Cuts at: 4583 4583 Size: 7279 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2556 2588 5348 5380 6861 6893 2556 Size: 32 2760 32 1481 32 2942 Fragments arranged by size: 2942 2760 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2134 4383 2125 Size: 9 2249 5021 Fragments arranged by size: 5021 2249 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7279 PsiI TTA'TAA Cuts at: 4017 4849 4017 Size: 832 6447 PspXI vC'TCGA_Gb Cuts at: 2573 2573 Size: 7279 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2948 2980 2948 Size: 32 7247 PstI C_TGCA'G Cuts at: 830 2223 3823 4631 830 Size: 1393 1600 808 3478 Fragments arranged by size: 3478 1600 1393 808 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7279 PvuII CAG'CTG Cuts at: 2218 2224 2218 Size: 6 7273 SacI G_AGCT'C Cuts at: 2235 2574 3483 2235 Size: 339 909 6031 Fragments arranged by size: 6031 909 339 SalI G'TCGA_C Cuts at: 1936 4621 1936 Size: 2685 4594 SbfI CC_TGCA'GG Cuts at: 4631 4631 Size: 7279 ScaI AGT'ACT Cuts at: 5527 7239 5527 Size: 1712 5567 SfcI C'TryA_G Cuts at: 826 1045 2219 3391 3804 3819 4563 4627 5762 Size: 219 1174 1172 413 15 744 64 1135 Cuts at: 5762 6443 6634 826 Size: 681 191 1471 Fragments arranged by size: 1471 1174 1172 1135 744 681 413 219 191 64 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7279 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7279 SmaI CCC'GGG Cuts at: 1872 4606 1872 Size: 2734 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2573 2936 3232 5383 6284 6546 Size: 197 926 630 363 296 2151 901 262 Cuts at: 6546 820 Size: 1553 Fragments arranged by size: 2151 1553 926 901 630 363 296 262 197 SnaBI TAC'GTA Cuts at: 493 5070 493 Size: 4577 2702 SpeI A'CTAG_T Cuts at: 152 3247 152 Size: 3095 4184 SphI G_CATG'C Cuts at: 1132 4637 1132 Size: 3505 3774 SspI AAT'ATT Cuts at: 5 52 2550 5203 5 Size: 47 2498 2653 2081 Fragments arranged by size: 2653 2498 2081 47 StuI AGG'CCT Cuts at: 1517 2273 2768 1517 Size: 756 495 6028 Fragments arranged by size: 6028 756 495 StyI C'CwwG_G Cuts at: 513 1065 2004 2029 3168 3442 4718 513 Size: 552 939 25 1139 274 1276 3074 Fragments arranged by size: 3074 1276 1139 939 552 274 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 2789 4111 5306 5323 5476 5661 Size: 1255 555 654 1322 1195 17 153 185 Cuts at: 5661 6106 325 Size: 445 1498 Fragments arranged by size: 1498 1322 1255 1195 654 555 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4074 4432 5525 Size: 274 80 33 51 1443 2097 358 1093 Cuts at: 5525 7237 96 Size: 1712 138 Fragments arranged by size: 2097 1712 1443 1093 358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7239 7271 7239 Size: 32 7247 Tth111I GACn'n_nGTC Cuts at: 1316 4205 1316 Size: 2889 4390 XbaI T'CTAG_A Cuts at: 3316 4615 3316 Size: 1299 5980 XhoI C'TCGA_G Cuts at: 1943 2573 1943 Size: 630 6649 XmaI C'CCGG_G Cuts at: 1870 4604 1870 Size: 2734 4545 XmnI GAAnn'nnTTC Cuts at: 2068 2669 5408 2068 Size: 601 2739 3939 Fragments arranged by size: 3939 2739 601 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5087 276 Size: 53 83 186 936 3553 2468 Fragments arranged by size: 3553 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI