(Linear) (Six-base) MAPSORT of: KIEE2134.seq Check: 6225 from: 1 to: 2574 KIEE2134.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2321 2574 Size: 2321 253 AccI GT'mk_AC Cuts at: 0 241 2574 Size: 241 2333 Acc65I G'GTAC_C Cuts at: 0 1338 2574 Size: 1338 1236 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 255 1391 2079 2298 2574 Size: 255 1136 688 219 276 Fragments arranged by size: 1136 688 276 255 219 AleI CACnn'nnGTG Cuts at: 0 2116 2574 Size: 2116 458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 353 2574 Size: 353 2221 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1418 1450 2574 Size: 1418 32 1124 Fragments arranged by size: 1418 1124 32 AlwNI CAG_nnn'CTG Cuts at: 0 184 222 351 2028 2062 2574 Size: 184 38 129 1677 34 512 Fragments arranged by size: 1677 512 184 129 38 34 ApoI r'AATT_y Cuts at: 0 59 426 507 1062 1141 2574 Size: 59 367 81 555 79 1433 Fragments arranged by size: 1433 555 367 81 79 59 AvaI C'yCGr_G Cuts at: 0 229 568 2574 Size: 229 339 2006 Fragments arranged by size: 2006 339 229 AvrII C'CTAG_G Cuts at: 0 24 2574 Size: 24 2550 BanI G'GyrC_C Cuts at: 0 796 1338 2300 2574 Size: 796 542 962 274 Fragments arranged by size: 962 796 542 274 BanII G_rGCy'C Cuts at: 0 230 569 575 1478 2574 Size: 230 339 6 903 1096 Fragments arranged by size: 1096 903 339 230 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 891 2574 Size: 891 1683 BlpI GC'TnA_GC Cuts at: 0 157 2574 Size: 157 2417 Bme1580I G_kGCm'C Cuts at: 0 2056 2574 Size: 2056 518 BmrI ACTGGGnnnn_n' Cuts at: 0 654 2382 2574 Size: 654 1728 192 Fragments arranged by size: 1728 654 192 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 52 2574 Size: 52 2522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 703 1333 2449 2574 Size: 244 459 630 1116 125 Fragments arranged by size: 1116 630 459 244 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 916 1248 2574 Size: 916 332 1326 Fragments arranged by size: 1326 916 332 BsaI GGTCTCn'nnnn_ Cuts at: 0 394 2574 Size: 394 2180 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1331 1361 2574 Size: 1331 30 1213 Fragments arranged by size: 1331 1213 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1371 2574 Size: 1371 1203 BseYI C'CCAG_C Cuts at: 0 1417 1804 2574 Size: 1417 387 770 Fragments arranged by size: 1417 770 387 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2140 2574 Size: 2140 434 BsiHKAI G_wGCw'C Cuts at: 0 38 230 569 1478 2358 2574 Size: 38 192 339 909 880 216 Fragments arranged by size: 909 880 339 216 192 38 BsmI GAATG_Cn' Cuts at: 0 2130 2574 Size: 2130 444 Bsp1286I G_dGCh'C Cuts at: 0 38 230 569 575 1478 2056 2358 2574 Size: 38 192 339 6 903 578 302 216 Fragments arranged by size: 903 578 339 302 216 192 38 6 BspHI T'CATG_A Cuts at: 0 1961 2574 Size: 1961 613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 287 1647 2321 2574 Size: 287 1360 674 253 Fragments arranged by size: 1360 674 287 253 BsrDI GCAATG_nn' Cuts at: 0 747 1112 2219 2574 Size: 747 365 1107 355 Fragments arranged by size: 1107 747 365 355 BsrFI r'CCGG_y Cuts at: 0 43 2574 Size: 43 2531 BsrGI T'GTAC_A Cuts at: 0 2069 2427 2574 Size: 2069 358 147 Fragments arranged by size: 2069 358 147 BssSI C'ACGA_G Cuts at: 0 914 2574 Size: 914 1660 BstBI TT'CG_AA Cuts at: 0 1060 2574 Size: 1060 1514 BstXI CCAn_nnnn'nTGG Cuts at: 0 2473 2574 Size: 2473 101 BstYI r'GATC_y Cuts at: 0 450 825 879 1167 1652 2460 2574 Size: 450 375 54 288 485 808 114 Fragments arranged by size: 808 485 450 375 288 114 54 BstZ17I GTA'TAC Cuts at: 0 242 2574 Size: 242 2332 Bsu36I CC'TnA_GG Cuts at: 0 1079 2456 2574 Size: 1079 1377 118 Fragments arranged by size: 1377 1079 118 BtgI C'CryG_G Cuts at: 0 1874 2574 Size: 1874 700 BtsI GCAGTG_nn' Cuts at: 0 182 2574 Size: 182 2392 DraI TTT'AAA Cuts at: 0 431 1364 2574 Size: 431 933 1210 Fragments arranged by size: 1210 933 431 DraIII CAC_nnn'GTG Cuts at: 0 1714 2574 Size: 1714 860 EaeI y'GGCC_r Cuts at: 0 41 81 854 2574 Size: 41 40 773 1720 Fragments arranged by size: 1720 773 41 40 EarI CTCTTCn'nnn_ Cuts at: 0 1867 1886 2574 Size: 1867 19 688 Fragments arranged by size: 1867 688 19 EcoICRI GAG'CTC Cuts at: 0 228 567 1476 2574 Size: 228 339 909 1098 Fragments arranged by size: 1098 909 339 228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 255 703 1333 1391 2079 2298 2449 Size: 244 11 448 630 58 688 219 151 Cuts at: 2449 2574 Size: 125 Fragments arranged by size: 688 630 448 244 219 151 125 58 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 115 2574 Size: 115 2459 EcoO109I rG'GnC_Cy Cuts at: 0 120 129 2378 2574 Size: 120 9 2249 196 Fragments arranged by size: 2249 196 120 9 EcoRI G'AATT_C Cuts at: 0 59 507 2574 Size: 59 448 2067 Fragments arranged by size: 2067 448 59 EcoRV GAT'ATC Cuts at: 0 1221 2574 Size: 1221 1353 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 285 339 2574 Size: 285 54 2235 Fragments arranged by size: 2235 285 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 517 549 1152 1184 1580 1612 1831 1863 Size: 517 32 603 32 396 32 219 32 Cuts at: 1863 2186 2218 2250 2282 2391 2423 2574 Size: 323 32 32 32 109 32 151 Fragments arranged by size: 603 517 396 323 219 151 109 32 32 32 32 32 32 32 32 KpnI G_GTAC'C Cuts at: 0 1342 2574 Size: 1342 1232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 560 1628 2574 Size: 560 1068 946 Fragments arranged by size: 1068 946 560 MscI TGG'CCA Cuts at: 0 856 2574 Size: 856 1718 MslI CAynn'nnrTG Cuts at: 0 335 1790 2116 2365 2368 2574 Size: 335 1455 326 249 3 206 Fragments arranged by size: 1455 335 326 249 206 3 MspA1I CmG'CkG Cuts at: 0 213 219 2574 Size: 213 6 2355 Fragments arranged by size: 2355 213 6 PasI CC'CwG_GG Cuts at: 0 2049 2325 2574 Size: 2049 276 249 Fragments arranged by size: 2049 276 249 PflMI CCAn_nnn'nTGG Cuts at: 0 792 2028 2574 Size: 792 1236 546 Fragments arranged by size: 1236 792 546 PfoI T'CCnGG_A Cuts at: 0 1145 1655 1666 2231 2465 2574 Size: 1145 510 11 565 234 109 Fragments arranged by size: 1145 565 510 234 109 11 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 551 583 2574 Size: 551 32 1991 Fragments arranged by size: 1991 551 32 PpuMI rG'GwC_Cy Cuts at: 0 120 129 2378 2574 Size: 120 9 2249 196 Fragments arranged by size: 2249 196 120 9 PsiI TTA'TAA Cuts at: 0 2012 2574 Size: 2012 562 PspXI vC'TCGA_Gb Cuts at: 0 568 2574 Size: 568 2006 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 943 975 2574 Size: 943 32 1599 Fragments arranged by size: 1599 943 32 PstI C_TGCA'G Cuts at: 0 218 1818 2574 Size: 218 1600 756 Fragments arranged by size: 1600 756 218 PvuII CAG'CTG Cuts at: 0 213 219 2574 Size: 213 6 2355 Fragments arranged by size: 2355 213 6 SacI G_AGCT'C Cuts at: 0 230 569 1478 2574 Size: 230 339 909 1096 Fragments arranged by size: 1096 909 339 230 SfcI C'TryA_G Cuts at: 0 214 1386 1799 1814 2558 2574 Size: 214 1172 413 15 744 16 Fragments arranged by size: 1172 744 413 214 16 15 SmlI C'TyrA_G Cuts at: 0 568 931 1227 2574 Size: 568 363 296 1347 Fragments arranged by size: 1347 568 363 296 SpeI A'CTAG_T Cuts at: 0 1242 2574 Size: 1242 1332 SspI AAT'ATT Cuts at: 0 545 2574 Size: 545 2029 StuI AGG'CCT Cuts at: 0 268 763 2574 Size: 268 495 1811 Fragments arranged by size: 1811 495 268 StyI C'CwwG_G Cuts at: 0 24 1163 1437 2574 Size: 24 1139 274 1137 Fragments arranged by size: 1139 1137 274 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 130 784 2106 2574 Size: 130 654 1322 468 Fragments arranged by size: 1322 654 468 130 TatI w'GTAC_w Cuts at: 0 2069 2427 2574 Size: 2069 358 147 Fragments arranged by size: 2069 358 147 Tth111I GACn'n_nGTC Cuts at: 0 2200 2574 Size: 2200 374 XbaI T'CTAG_A Cuts at: 0 1311 2574 Size: 1311 1263 XhoI C'TCGA_G Cuts at: 0 568 2574 Size: 568 2006 XmnI GAAnn'nnTTC Cuts at: 0 63 664 2574 Size: 63 601 1910 Fragments arranged by size: 1910 601 63 Enzymes that do cut: AarI AccI Acc65I AcuI AleI AlfI AloI AlwNI ApoI AvaI AvrII BanI BanII BbsI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I KpnI MmeI MscI MslI MspA1I PasI PflMI PfoI PpiI PpuMI PsiI PspXI PsrI PstI PvuII SacI SfcI SmlI SpeI SspI StuI StyI TaqII TatI Tth111I XbaI XhoI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BstAPI BstEII BtgZI ClaI CspCI DrdI EagI EciI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PspOMI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TstI XcmI XmaI ZraI