Restriction map for pFN21AE9290
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BcgI        BciVI       
BcnI        BfrI        BglI        BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRII      EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tth111I     TthHB8I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AlwNI         282  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         666  GGTCC                G^G[AT]CC
BbvCI         303  CCTCAGC              CCTCAGC(-5/-2)
BclI          734  TGATCA               T^GATCA
BglII         119  AGATCT               A^GATCT
BpmI          159  CTCCAG               CTGGAG(16/14)
BsaI          156  GGTCTC               GGTCTC(1/5)
BsmBI         919  CGTCTC               CGTCTC(1/5)
BsrDI         511  GCAATG               GCAATG(2/0)
BsrI          896  ACTGG                ACTGG(1/-1)
DraII         402  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          633  CCATGG               C^C[GA][CT]GG
EcoO109I      402  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         576  GATATC               GAT^ATC
FbaI          734  TGATCA               T^GATCA
HaeIII        404  GGCC                 GG^CC
HincII        130  GTTGAC               GT[CT]^[GA]AC
HindII        130  GTTGAC               GT[CT]^[GA]AC
HindIII       677  AAGCTT               A^AGCTT
MaeII         482  ACGT                 A^CGT
MslI          193  CATTGGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        285  CAGCTG               C[AC]G^C[GT]G
MwoI          762  GCTGGAAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          633  CCATGG               C^CATGG
NheI          543  GCTAGC               G^CTAGC
NlaIV         402  GGGGCC               GG[ACGT]^[ACGT]CC
PflMI          95  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          531  GACTC                GAGTC(4/5)
PvuII         285  CAGCTG               CAG^CTG
SfaNI         624  GATGC                GCATC(5/9)
TspRI         144  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I         95  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           29  CCATTCTAATAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          154  GCGG                 CCGC(-3/-1)
              882  GCGG                
AlwI          652  GATCC                GGATC(4/5)
              643  GGATC               
BbsI          778  GTCTTC               GAAGAC(2/6)
              952  GTCTTC              
BbvI          284  GCAGC                GCAGC(8/12)
              627  GCAGC               
BfaI          299  CTAG                 C^TAG
              544  CTAG                
BpuAI         778  GTCTTC               GAAGAC(2/6)
              952  GTCTTC              
BsaJI         633  CCATGG               C^C[ACGT][ACGT]GG
              662  CCAAGG              
BseRI         366  GAGGAG               GAGGAG(10/8)
              504  GAGGAG              
BsiYI          95  CCATTGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              834  CCTGATTTTGG         
BslI           95  CCATTGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              834  CCTGATTTTGG         
BstF5I        831  CATCC                GGATG(2/0)
              447  GGATG               
Cfr13I        403  GGGCC                G^G[ACGT]CC
              666  GGTCC               
EcoT14I       633  CCATGG               C^C[AT][AT]GG
              662  CCAAGG              
FokI          831  CATCC                GGATG(9/13)
              447  GGATG               
HphI          390  GGTGA                GGTGA(8/7)
              900  GGTGA               
MaeI          299  CTAG                 C^TAG
              544  CTAG                
MseI          374  TTAA                 T^TAA
              592  TTAA                
NspI          308  GCATGT               [GA]CATG^[CT]
              885  GCATGT              
Sau96I        403  GGGCC                G^G[ACGT]CC
              666  GGTCC               
SmlI          616  CTCAAG               C^T[CT][GA]AG
              647  CTCAAG              
StyI          633  CCATGG               C^C[AT][AT]GG
              662  CCAAGG              
TfiI          213  GAATC                G^A[AT]TC
              929  GATTC               
Tru9I         374  TTAA                 T^TAA
              592  TTAA                
TseI          284  GCAGC                G^C[AT]GC
              627  GCAGC               
Tsp45I        484  GTGAC                ^GT[GC]AC
              691  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsmAI       
BstYI       Cac8I       DdeI        DpnII       Fnu4HI      
HinfI       ItaI        MaeIII      MboI        MboII       
MflI        MnlI        NdeII       NlaIII      RsaI        
Sau3AI      Tsp509I     XhoII       
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