################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvaI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AlwNI 282 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 666 GGTCC G^G[AT]CC BbvCI 303 CCTCAGC CCTCAGC(-5/-2) BclI 734 TGATCA T^GATCA BglII 119 AGATCT A^GATCT BpmI 159 CTCCAG CTGGAG(16/14) BsaI 156 GGTCTC GGTCTC(1/5) BsmBI 919 CGTCTC CGTCTC(1/5) BsrDI 511 GCAATG GCAATG(2/0) BsrI 896 ACTGG ACTGG(1/-1) DraII 402 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 633 CCATGG C^C[GA][CT]GG EcoO109I 402 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 576 GATATC GAT^ATC FbaI 734 TGATCA T^GATCA HaeIII 404 GGCC GG^CC HincII 130 GTTGAC GT[CT]^[GA]AC HindII 130 GTTGAC GT[CT]^[GA]AC HindIII 677 AAGCTT A^AGCTT MaeII 482 ACGT A^CGT MslI 193 CATTGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 285 CAGCTG C[AC]G^C[GT]G MwoI 762 GCTGGAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 633 CCATGG C^CATGG NheI 543 GCTAGC G^CTAGC NlaIV 402 GGGGCC GG[ACGT]^[ACGT]CC PflMI 95 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 531 GACTC GAGTC(4/5) PvuII 285 CAGCTG CAG^CTG SfaNI 624 GATGC GCATC(5/9) TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 95 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 29 CCATTCTAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 154 GCGG CCGC(-3/-1) 882 GCGG AlwI 652 GATCC GGATC(4/5) 643 GGATC BbsI 778 GTCTTC GAAGAC(2/6) 952 GTCTTC BbvI 284 GCAGC GCAGC(8/12) 627 GCAGC BfaI 299 CTAG C^TAG 544 CTAG BpuAI 778 GTCTTC GAAGAC(2/6) 952 GTCTTC BsaJI 633 CCATGG C^C[ACGT][ACGT]GG 662 CCAAGG BseRI 366 GAGGAG GAGGAG(10/8) 504 GAGGAG BsiYI 95 CCATTGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCTGATTTTGG BslI 95 CCATTGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCTGATTTTGG BstF5I 831 CATCC GGATG(2/0) 447 GGATG Cfr13I 403 GGGCC G^G[ACGT]CC 666 GGTCC EcoT14I 633 CCATGG C^C[AT][AT]GG 662 CCAAGG FokI 831 CATCC GGATG(9/13) 447 GGATG HphI 390 GGTGA GGTGA(8/7) 900 GGTGA MaeI 299 CTAG C^TAG 544 CTAG MseI 374 TTAA T^TAA 592 TTAA NspI 308 GCATGT [GA]CATG^[CT] 885 GCATGT Sau96I 403 GGGCC G^G[ACGT]CC 666 GGTCC SmlI 616 CTCAAG C^T[CT][GA]AG 647 CTCAAG StyI 633 CCATGG C^C[AT][AT]GG 662 CCAAGG TfiI 213 GAATC G^A[AT]TC 929 GATTC Tru9I 374 TTAA T^TAA 592 TTAA TseI 284 GCAGC G^C[AT]GC 627 GCAGC Tsp45I 484 GTGAC ^GT[GC]AC 691 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AcsI AfaI AluI ApoI BsmAI BstYI Cac8I DdeI DpnII Fnu4HI HinfI ItaI MaeIII MboI MboII MflI MnlI NdeII NlaIII RsaI Sau3AI Tsp509I XhoII