Restriction map for pFN21AE9290
 (Circular) (Six-base) MAPSORT of: pFN21AE9290.seq  Check: 6215  from: 1  to: 5662



pFN21AE9290  5662 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:55  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3472     278 
   Size:         53      83     186     936    1936    2468

  Fragments arranged by size: 

                2468    1936     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3005    1937 
   Size:       1068    4594
 
Acc65I G'GTAC_C

Cuts at:   2983    2983 
   Size:       5662
 
AclI AA'CG_TT

Cuts at:   1563    3789    4162    1563 
   Size:       2226     373    3063

  Fragments arranged by size: 

                3063    2226     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3725    5109    5377    1632 
   Size:       2093    1384     268    1917

  Fragments arranged by size: 

                2093    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5465
 
AflIII A'CryG_T

Cuts at:   1203    4561    5538    1203 
   Size:       3358     977    1327

  Fragments arranged by size: 

                3358    1327     977
 
AgeI A'CCGG_T

Cuts at:   4493    4493 
   Size:       5662
 
AhdI GACnn_n'nnGTC

Cuts at:   4391    4391 
   Size:       5662
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5662
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5662
 
AlwNI CAG_nnn'CTG

Cuts at:   2292    3405    4977    2292 
   Size:       1113    1572    2977

  Fragments arranged by size: 

                2977    1572    1113
 
ApaLI G'TGCA_C

Cuts at:   3719    4875    3719 
   Size:       1156    4506
 
ApoI r'AATT_y

Cuts at:   1493    2065    2095    2142    2971    3197    3349    5458    1493 
   Size:        572      30      47     829     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     829     572     226     152      47      30
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4216     160 
   Size:        872    3184    1606

  Fragments arranged by size: 

                3184    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5662
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2987    1870 
   Size:         73    1044    4545

  Fragments arranged by size: 

                4545    1044      73
 
BamHI G'GATC_C

Cuts at:   2992    3359    2992 
   Size:        367    5295
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2983    4438     618 
   Size:        325     217     627    1196    1455    1842

  Fragments arranged by size: 

                1842    1455    1196     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2981    1391 
   Size:       1590    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2776    2950     928 
   Size:        971     877     174    3640

  Fragments arranged by size: 

                3640     971     877     174
 
BbvCI CC'TCA_GC

Cuts at:   2309    2309 
   Size:       5662
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3853    3887    1903 
   Size:         34    1916      34    3678

  Fragments arranged by size: 

                3678    1916      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3554    4764    3554 
   Size:       1210    4452
 
BclI T'GATC_A

Cuts at:   1548    2739    1548 
   Size:       1191    4471
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4273     136 
   Size:        107     122      71     631     605    2601    1525

  Fragments arranged by size: 

                2601    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2124    5657    2124 
   Size:       3533    2129
 
BlpI GC'TnA_GC

Cuts at:   3079    3079 
   Size:       5662
 
Bme1580I G_kGCm'C

Cuts at:   1790    3723    4879    1790 
   Size:       1933    1156    2573

  Fragments arranged by size: 

                2573    1933    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5662
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2911    3393    4351 
   Size:        416      53      83     399      24    1494     482     958

Cuts at:   4351     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1494     958     482     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    2552    1056 
   Size:       1496    4166
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2149    4322    1239 
   Size:        910    2173    2579

  Fragments arranged by size: 

                2579    2173     910
 
Bpu10I CC'TnA_GC

Cuts at:   2309    2327    2309 
   Size:         18    5644
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2606    2637    3787    4652    4950    2606 
   Size:         31    1150     865     298    3318

  Fragments arranged by size: 

                3318    1150     865     298      31
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2167    4325     882 
   Size:        617     668    2158    2219

  Fragments arranged by size: 

                2219    2158     668     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3453     493 
   Size:        711    2249    2702

  Fragments arranged by size: 

                2702    2249     711
 
BsaBI GATnn'nnATC

Cuts at:   5656    5656 
   Size:       5662
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3469    3851    4486     275 
   Size:         53      83     186     936    1936     382     635    1451

  Fragments arranged by size: 

                1936    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4095    4493    4767    4914    5325    4095 
   Size:        398     274     147     411    4432

  Fragments arranged by size: 

                4432     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5437

  Fragments arranged by size: 

                5437     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2386    2524    1186 
   Size:       1200     138    4324

  Fragments arranged by size: 

                4324    1200     138
 
BseYI C'CCAG_C

Cuts at:   2660    4865    5361    2660 
   Size:       2205     496    2961

  Fragments arranged by size: 

                2961    2205     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5089
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3112    3873    4901    1540 
   Size:        275     186    1111     761    1028    2301

  Fragments arranged by size: 

                2301    1111    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3723    3808    4879    3723 
   Size:         85    1071    4506

  Fragments arranged by size: 

                4506    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3173    3266    3173 
   Size:         93    5569
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2930    2930 
   Size:       5662
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2981    3723    3808    4879    1391 
   Size:        399    1191     742      85    1071    2174

  Fragments arranged by size: 

                2174    1191    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5325    5325 
   Size:       5662
 
BspHI T'CATG_A

Cuts at:   3551    5281    3551 
   Size:       1730    3932
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3017     844 
   Size:       2173    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3549    1394 
   Size:        429    1726    3507

  Fragments arranged by size: 

                3507    1726     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2523    4157    4331      65 
   Size:       1183    1275    1634     174    1396

  Fragments arranged by size: 

                1634    1396    1275    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4306    4493    1700 
   Size:        110    2496     187    2869

  Fragments arranged by size: 

                2869    2496     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5662
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5662
 
BssSI C'ACGA_G

Cuts at:   3722    4734    3722 
   Size:       1012    4650
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5662
 
BstBI TT'CG_AA

Cuts at:   4466    4466 
   Size:       5662
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5662
 
BstYI r'GATC_y

Cuts at:   1972    2124    2648    2656    2992    3359    3744    3761    5213 
   Size:        152     524       8     336     367     385      17    1452

Cuts at:   5213    5299    5311    5657    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     524     385     367     346     336     152
                  86      17      12       8
 
BtgI C'CryG_G

Cuts at:    513    1065    2638     513 
   Size:        552    1573    3537

  Fragments arranged by size: 

                3537    1573     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4923

  Fragments arranged by size: 

                4923     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2148    3178    3972    3992    2148 
   Size:       1030     794      20    3818

  Fragments arranged by size: 

                3818    1030     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5662
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5627
 
DraI TTT'AAA

Cuts at:   2966    2966 
   Size:       5662
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4669     809 
   Size:        507    3353    1802

  Fragments arranged by size: 

                3353    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3109    3998    5627 
   Size:         54    1425      50     275      18    1279     889    1629

Cuts at:   5627       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1279     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3109    1537 
   Size:        275    1297    4090

  Fragments arranged by size: 

                4090    1297     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3592    1353 
   Size:       2239    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4249    4623    4769     387 
   Size:       1064     138    2660     374     146    1280

  Fragments arranged by size: 

                2660    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2149    3725    4322    5109    5377    1239 
   Size:        393     517    1576     597     787     268    1524

  Fragments arranged by size: 

                1576    1524     787     597     517     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2408    2408 
   Size:       5662
 
EcoRI G'AATT_C

Cuts at:   2971    3349    2971 
   Size:        378    5284
 
EcoRV GAT'ATC

Cuts at:   2583    2583 
   Size:       5662
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2146    2146 
   Size:       5662
 
FspI TGC'GCA

Cuts at:   4168    4168 
   Size:       5662
 
HaeII r_GCGC'y

Cuts at:   1678    4809    1678 
   Size:       3131    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2109    2141    4303    4335    4377 
   Size:         32     135      32     537      32    2162      32      42

Cuts at:   4377    4409    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2162     537     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2137    3006    3252     669 
   Size:        988     281     199     869     246    3079

  Fragments arranged by size: 

                3079     988     869     281     246     199
 
HindIII A'AGCT_T

Cuts at:    748    2682     748 
   Size:       1934    3728
 
HpaI GTT'AAC

Cuts at:   3252    3252 
   Size:       5662
 
KpnI G_GTAC'C

Cuts at:   2987    2987 
   Size:       5662
 
MfeI C'AATT_G

Cuts at:   3261    3261 
   Size:       5662
 
MluI A'CGCG_T

Cuts at:   5538    5538 
   Size:       5662
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2370    4776    4960    1305 
   Size:       1065    2406     184    2007

  Fragments arranged by size: 

                2406    2007    1065     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3840

  Fragments arranged by size: 

                3840    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2202    3620    3979    4138     518 
   Size:        648    1036    1418     359     159    2042

  Fragments arranged by size: 

                2042    1418    1036     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2292    3078    3755    4903    5148    1606 
   Size:        686     786     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     786     686     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2638     513 
   Size:        552    1573    3537

  Fragments arranged by size: 

                3537    1573     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5662
 
NheI G'CTAG_C

Cuts at:   1052    2548    1052 
   Size:       1496    4166
 
NotI GC'GGCC_GC

Cuts at:   3109    3109 
   Size:       5662
 
NspI r_CATG'y

Cuts at:   1132    1229    2317    2894    3020    4565    1132 
   Size:         97    1088     577     126    1545    2229

  Fragments arranged by size: 

                2229    1545    1088     577     126      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5662
 
PciI A'CATG_T

Cuts at:   4561    4561 
   Size:       5662
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2106    1108 
   Size:        998    4664
 
PmeI GTTT'AAAC

Cuts at:   2966    2966 
   Size:       5662
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3731    3763    5244    5276    3731 
   Size:         32    1481      32    4117

  Fragments arranged by size: 

                4117    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5662
 
PsiI TTA'TAA

Cuts at:   2259    3232    2259 
   Size:        973    4689
 
PstI C_TGCA'G

Cuts at:    830    3014     830 
   Size:       2184    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5662
 
PvuII CAG'CTG

Cuts at:   2292    2292 
   Size:       5662
 
SalI G'TCGA_C

Cuts at:   1936    3004    1936 
   Size:       1068    4594
 
SbfI CC_TGCA'GG

Cuts at:   3014    3014 
   Size:       5662
 
ScaI AGT'ACT

Cuts at:   3910    5622    3910 
   Size:       1712    3950
 
SfcI C'TryA_G

Cuts at:    826    1045    3010    4145    4826    5017     826 
   Size:        219    1965    1135     681     191    1471

  Fragments arranged by size: 

                1965    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5662
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5662
 
SmaI CCC'GGG

Cuts at:   1872    2989    1872 
   Size:       1117    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2621    2652    3766    4667    4929     820 
   Size:        197     926     678      31    1114     901     262    1553

  Fragments arranged by size: 

                1553    1114     926     901     678     262     197      31
            
 
SnaBI TAC'GTA

Cuts at:    493    3453     493 
   Size:       2960    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5662
 
SphI G_CATG'C

Cuts at:   1132    3020    1132 
   Size:       1888    3774
 
SspI AAT'ATT

Cuts at:      5      52    3586       5 
   Size:         47    3534    2081

  Fragments arranged by size: 

                3534    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5662
 
StyI C'CwwG_G

Cuts at:    513    1065    2638    2667    3101     513 
   Size:        552    1573      29     434    3074

  Fragments arranged by size: 

                3074    1573     552     434      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2173    2844    2893    3689    3706    3859    4044 
   Size:       1255     593     671      49     796      17     153     185

Cuts at:   4044    4489     325 
   Size:        445    1498

  Fragments arranged by size: 

                1498    1255     796     671     593     445     185     153
                  49      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3908    5620      96 
   Size:        274      80      33      51    1443    1931    1712     138

  Fragments arranged by size: 

                1931    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5622    5654    5622 
   Size:         32    5630
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5662
 
XbaI T'CTAG_A

Cuts at:   2998    2998 
   Size:       5662
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2041    2041 
   Size:       5662
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5662
 
XmaI C'CCGG_G

Cuts at:   1870    2987    1870 
   Size:       1117    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3791    3791 
   Size:       5662
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3470     276 
   Size:         53      83     186     936    1936    2468

  Fragments arranged by size: 

                2468    1936     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI
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