(Linear) (Six-base) MAPSORT of: KIEE5974.seq Check: 3281 from: 1 to: 936 KIEE5974.orf With 182 enzymes: SgfI * December 5, 2014 13:17 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 936 Size: 294 642 AflIII A'CryG_T Cuts at: 0 218 936 Size: 218 718 AlwNI CAG_nnn'CTG Cuts at: 0 75 936 Size: 75 861 ApaLI G'TGCA_C Cuts at: 0 324 936 Size: 324 612 ApoI r'AATT_y Cuts at: 0 886 936 Size: 886 50 AvaI C'yCGr_G Cuts at: 0 411 936 Size: 411 525 BanII G_rGCy'C Cuts at: 0 193 808 936 Size: 193 615 128 Fragments arranged by size: 615 193 128 BbsI GAAGACnn'nnnn_ Cuts at: 0 741 936 Size: 741 195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 261 295 936 Size: 261 34 641 Fragments arranged by size: 641 261 34 BclI T'GATC_A Cuts at: 0 470 936 Size: 470 466 BglI GCCn_nnn'nGGC Cuts at: 0 352 936 Size: 352 584 BglII A'GATC_T Cuts at: 0 209 936 Size: 209 727 Bme1580I G_kGCm'C Cuts at: 0 328 936 Size: 328 608 BmgBI CAC'GTC Cuts at: 0 399 936 Size: 399 537 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 168 226 244 265 409 474 723 936 Size: 168 58 18 21 144 65 249 213 Fragments arranged by size: 249 213 168 144 65 58 21 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 936 Size: 538 398 BsaAI yAC'GTr Cuts at: 0 219 369 936 Size: 219 150 567 Fragments arranged by size: 567 219 150 BsaHI Gr'CG_yC Cuts at: 0 896 936 Size: 896 40 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 199 229 936 Size: 199 30 707 Fragments arranged by size: 707 199 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 76 247 936 Size: 76 171 689 Fragments arranged by size: 689 171 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 549 936 Size: 549 387 BsiEI CG_ry'CG Cuts at: 0 363 936 Size: 363 573 BsiHKAI G_wGCw'C Cuts at: 0 193 328 808 936 Size: 193 135 480 128 Fragments arranged by size: 480 193 135 128 Bsp1286I G_dGCh'C Cuts at: 0 193 328 808 936 Size: 193 135 480 128 Fragments arranged by size: 480 193 135 128 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 83 727 936 Size: 83 644 209 Fragments arranged by size: 644 209 83 BsrBI CCG'CTC Cuts at: 0 630 936 Size: 630 306 BsrFI r'CCGG_y Cuts at: 0 694 936 Size: 694 242 BsrGI T'GTAC_A Cuts at: 0 830 936 Size: 830 106 BstAPI GCAn_nnn'nTGC Cuts at: 0 75 936 Size: 75 861 BstEII G'GTnAC_C Cuts at: 0 741 936 Size: 741 195 BstXI CCAn_nnnn'nTGG Cuts at: 0 243 936 Size: 243 693 BstYI r'GATC_y Cuts at: 0 209 936 Size: 209 727 Bsu36I CC'TnA_GG Cuts at: 0 874 936 Size: 874 62 BtgI C'CryG_G Cuts at: 0 53 350 737 936 Size: 53 297 387 199 Fragments arranged by size: 387 297 199 53 BtsI GCAGTG_nn' Cuts at: 0 578 936 Size: 578 358 DraIII CAC_nnn'GTG Cuts at: 0 332 936 Size: 332 604 EaeI y'GGCC_r Cuts at: 0 128 371 635 689 936 Size: 128 243 264 54 247 Fragments arranged by size: 264 247 243 128 54 EarI CTCTTCn'nnn_ Cuts at: 0 85 94 936 Size: 85 9 842 Fragments arranged by size: 842 85 9 EcoICRI GAG'CTC Cuts at: 0 191 806 936 Size: 191 615 130 Fragments arranged by size: 615 191 130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 168 226 244 265 294 409 474 723 Size: 168 58 18 21 29 115 65 249 Cuts at: 723 936 Size: 213 Fragments arranged by size: 249 213 168 115 65 58 29 21 18 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 915 936 Size: 915 21 FspI TGC'GCA Cuts at: 0 679 936 Size: 679 257 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 536 833 936 Size: 536 297 103 Fragments arranged by size: 536 297 103 MscI TGG'CCA Cuts at: 0 130 373 637 936 Size: 130 243 264 299 Fragments arranged by size: 299 264 243 130 MslI CAynn'nnrTG Cuts at: 0 40 466 619 936 Size: 40 426 153 317 Fragments arranged by size: 426 317 153 40 MspA1I CmG'CkG Cuts at: 0 387 429 936 Size: 387 42 507 Fragments arranged by size: 507 387 42 NcoI C'CATG_G Cuts at: 0 350 936 Size: 350 586 PasI CC'CwG_GG Cuts at: 0 61 936 Size: 61 875 PflMI CCAn_nnn'nTGG Cuts at: 0 432 936 Size: 432 504 PfoI T'CCnGG_A Cuts at: 0 203 936 Size: 203 733 PstI C_TGCA'G Cuts at: 0 71 585 936 Size: 71 514 351 Fragments arranged by size: 514 351 71 PvuII CAG'CTG Cuts at: 0 429 936 Size: 429 507 SacI G_AGCT'C Cuts at: 0 193 808 936 Size: 193 615 128 Fragments arranged by size: 615 193 128 SapI GCTCTTCn'nnn_ Cuts at: 0 85 936 Size: 85 851 SfcI C'TryA_G Cuts at: 0 67 581 936 Size: 67 514 355 Fragments arranged by size: 514 355 67 SfiI GGCCn_nnn'nGGCC Cuts at: 0 352 936 Size: 352 584 SmaI CCC'GGG Cuts at: 0 413 936 Size: 413 523 SmlI C'TyrA_G Cuts at: 0 553 936 Size: 553 383 StuI AGG'CCT Cuts at: 0 910 936 Size: 910 26 StyI C'CwwG_G Cuts at: 0 20 350 911 936 Size: 20 330 561 25 Fragments arranged by size: 561 330 25 20 TatI w'GTAC_w Cuts at: 0 176 830 936 Size: 176 654 106 Fragments arranged by size: 654 176 106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 201 555 585 936 Size: 201 354 30 351 Fragments arranged by size: 354 351 201 30 XmaI C'CCGG_G Cuts at: 0 411 936 Size: 411 525 XmnI GAAnn'nnTTC Cuts at: 0 526 936 Size: 526 410 Enzymes that do cut: AcuI AflIII AlwNI ApaLI ApoI AvaI BanII BbsI BcgI BclI BglI BglII Bme1580I BmgBI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI FalI FspI MmeI MscI MslI MspA1I NcoI PasI PflMI PfoI PstI PvuII SacI SapI SfcI SfiI SmaI SmlI StuI StyI TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BfrBI BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaWI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI ZraI