################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflII 867 CTTAAG C^TTAAG AlwI 316 GGATC GGATC(4/5) AlwNI 432 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG BbsI 220 GTCTTC GAAGAC(2/6) BbvI 546 GCTGC GCAGC(8/12) BfrI 867 CTTAAG C^TTAAG BpmI 504 CTCCAG CTGGAG(16/14) BpuAI 220 GTCTTC GAAGAC(2/6) BsmAI 257 GAGAC GTCTC(1/5) BspHI 398 TCATGA T^CATGA BspMI 879 GCAGGT ACCTGC(4/8) BsrDI 246 CATTGC GCAATG(2/0) BsrI 489 ACTGG ACTGG(1/-1) BssKI 437 CCTGG ^CC[ACGT]GG BstF5I 654 CATCC GGATG(2/0) BstNI 437 CCTGG CC^[AT]GG DraII 890 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 284 CCATGG C^C[GA][CT]GG EcoO109I 890 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 437 CCTGG ^CC[AT]GG Fnu4HI 546 GCTGC GC^[ACGT]GC FokI 654 CATCC GGATG(9/13) HincII 135 GTTAAC GT[CT]^[GA]AC HindII 135 GTTAAC GT[CT]^[GA]AC HpaI 135 GTTAAC GTT^AAC ItaI 546 GCTGC GC^[ACGT]GC MvaI 437 CCTGG CC^[AT]GG MwoI 280 GCTTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 284 CCATGG C^CATGG NdeI 724 CATATG CA^TATG NheI 597 GCTAGC G^CTAGC PleI 502 GACTC GAGTC(4/5) RcaI 398 TCATGA T^CATGA ScrFI 437 CCTGG CC^[ACGT]GG SfcI 251 CTACAG C^T[GA][CT]AG SmlI 867 CTTAAG C^T[CT][GA]AG TseI 546 GCTGC G^C[AT]GC Tsp45I 500 GTGAC ^GT[GC]AC TspRI 818 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 576 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 433 AGCT AG^CT 581 AGCT AocI 180 CCTCAGG CC^T[ACGT]AGG 894 CCTTAGG Asp700I 25 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 325 GAATTTTTTC BsaJI 284 CCATGG C^C[ACGT][ACGT]GG 629 CCTTGG BsiYI 483 CCTCCAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 686 CCCAGCAAAGG BslI 483 CCTCCAACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 686 CCCAGCAAAGG Bsu36I 180 CCTCAGG CC^T[ACGT]AGG 894 CCTTAGG Eco81I 180 CCTCAGG CC^T[ACGT]AGG 894 CCTTAGG EcoRI 25 GAATTC G^AATTC 901 GAATTC EcoT14I 284 CCATGG C^C[AT][AT]GG 629 CCTTGG HphI 882 GGTGA GGTGA(8/7) 905 TCACC SspI 148 AATATT AAT^ATT 915 AATATT StyI 284 CCATGG C^C[AT][AT]GG 629 CCTTGG XmnI 25 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 325 GAATTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AcsI ApoI BclI BfaI Cac8I Cfr13I DdeI DpnII FbaI HaeIII HinfI MaeI MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII Sau3AI Sau96I TfiI Tru9I Tsp509I