(Circular) (Six-base) MAPSORT of: pFN21AE5953.seq Check: 1055 from: 1 to: 5641 pFN21AE5953 5641 bp With 182 enzymes: SgfI * December 8, 2014 13:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 5641 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2559 3704 5088 5356 1632 Size: 927 1145 1384 268 1917 Fragments arranged by size: 1917 1384 1145 927 268 AflII C'TTAA_G Cuts at: 820 1017 2929 820 Size: 197 1912 3532 Fragments arranged by size: 3532 1912 197 AflIII A'CryG_T Cuts at: 1203 2728 4540 5517 1203 Size: 1525 1812 977 1327 Fragments arranged by size: 1812 1525 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2228 1166 Size: 1062 4579 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2496 1184 Size: 1312 4329 AlwNI CAG_nnn'CTG Cuts at: 3384 4956 3384 Size: 1572 4069 ApaLI G'TGCA_C Cuts at: 2508 3698 4854 2508 Size: 1190 1156 3295 Fragments arranged by size: 3295 1190 1156 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 AvrII C'CTAG_G Cuts at: 2041 2041 Size: 5641 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2068 2469 2899 2960 1391 Size: 677 401 430 61 4072 Fragments arranged by size: 4072 677 430 401 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2572 928 Size: 971 673 3997 Fragments arranged by size: 3997 971 673 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4252 136 Size: 107 122 71 631 605 2580 1525 Fragments arranged by size: 2580 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2298 5636 2298 Size: 3338 2303 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 2235 2512 3702 4858 1790 Size: 445 277 1190 1156 2573 Fragments arranged by size: 2573 1190 1156 445 277 BmgBI CAC'GTC Cuts at: 1318 2209 1318 Size: 891 4750 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3372 4330 442 Size: 416 53 83 399 24 1955 958 1753 Fragments arranged by size: 1955 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4301 1239 Size: 3062 2579 Bpu10I CC'TnA_GC Cuts at: 2186 2489 2186 Size: 303 5338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2660 2773 4304 882 Size: 617 1161 113 1531 2219 Fragments arranged by size: 2219 1531 1161 617 113 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2258 4074 4472 4746 4893 5304 2258 Size: 1816 398 274 147 411 2595 Fragments arranged by size: 2595 1816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5641 BseYI C'CCAG_C Cuts at: 4844 5340 4844 Size: 496 5145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 2475 2660 1163 Size: 573 579 160 185 4144 Fragments arranged by size: 4144 579 573 185 160 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2512 3702 3787 4858 2512 Size: 1190 85 1071 3295 Fragments arranged by size: 3295 1190 1071 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2068 2235 2469 2512 2899 2960 3702 Size: 399 278 167 234 43 387 61 742 Cuts at: 3702 3787 4858 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 278 234 167 85 61 43 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 2082 2130 2403 3530 5260 2082 Size: 48 273 1127 1730 2463 Fragments arranged by size: 2463 1730 1127 273 48 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2726 2996 844 Size: 1882 270 3489 Fragments arranged by size: 3489 1882 270 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2157 4285 4472 1700 Size: 110 347 2128 187 2869 Fragments arranged by size: 2869 2128 347 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 2513 3701 4713 2513 Size: 1188 1012 3441 Fragments arranged by size: 3441 1188 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 2583 4445 2583 Size: 1862 3779 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2298 2971 3338 3723 3740 5192 5278 5290 Size: 326 673 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 673 385 367 346 326 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2423 2423 Size: 5641 BtgI C'CryG_G Cuts at: 513 1065 2004 2349 513 Size: 552 939 345 3805 Fragments arranged by size: 3805 939 552 345 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 2520 1554 Size: 966 4675 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2576 4648 809 Size: 507 1260 2072 1802 Fragments arranged by size: 2072 1802 1260 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3088 3977 5606 Size: 54 1425 50 275 18 1258 889 1629 Cuts at: 5606 8 Size: 43 Fragments arranged by size: 1629 1425 1258 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2728 3571 1353 Size: 731 644 843 3423 Fragments arranged by size: 3423 843 731 644 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2559 3704 4301 5088 5356 1239 Size: 393 927 1145 597 787 268 1524 Fragments arranged by size: 1524 1145 927 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2539 2539 Size: 5641 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 EcoRV GAT'ATC Cuts at: 2393 2393 Size: 5641 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2725 2725 Size: 5641 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2207 2228 2402 3599 3958 4117 Size: 648 1005 36 21 174 1197 359 159 Cuts at: 4117 518 Size: 2042 Fragments arranged by size: 2042 1197 1005 648 359 174 159 36 21 MspA1I CmG'CkG Cuts at: 1606 2416 2459 3057 3734 4882 5127 1606 Size: 810 43 598 677 1148 245 2120 Fragments arranged by size: 2120 1148 810 677 598 245 43 NcoI C'CATG_G Cuts at: 513 1065 2004 2349 513 Size: 552 939 345 3805 Fragments arranged by size: 3805 939 552 345 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2683 2732 2999 4544 1132 Size: 97 1454 49 267 1545 2229 Fragments arranged by size: 2229 1545 1454 267 97 49 PasI CC'CwG_GG Cuts at: 1348 2317 1348 Size: 969 4672 PciI A'CATG_T Cuts at: 2728 4540 2728 Size: 1812 3829 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PstI C_TGCA'G Cuts at: 830 2475 2993 830 Size: 1645 518 3478 Fragments arranged by size: 3478 1645 518 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2416 2459 2416 Size: 43 5598 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SapI GCTCTTCn'nnn_ Cuts at: 2728 2728 Size: 5641 SbfI CC_TGCA'GG Cuts at: 2475 2993 2475 Size: 518 5123 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2471 2688 2866 2989 4124 4805 4996 Size: 219 1426 217 178 123 1135 681 191 Cuts at: 4996 826 Size: 1471 Fragments arranged by size: 1471 1426 1135 681 219 217 191 178 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2929 3745 4646 4908 820 Size: 197 926 986 816 901 262 1553 Fragments arranged by size: 1553 986 926 901 816 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 2708 1517 Size: 1191 4450 StyI C'CwwG_G Cuts at: 513 1065 2004 2041 2349 3080 513 Size: 552 939 37 308 731 3074 Fragments arranged by size: 3074 939 731 552 308 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2309 2397 2441 3668 3685 3838 4023 Size: 1255 729 88 44 1227 17 153 185 Cuts at: 4023 4468 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1227 729 445 185 153 88 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 3887 5599 Size: 274 80 33 51 1443 198 1712 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1712 1712 1443 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 2434 1316 Size: 1118 4523 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI