(Circular) (Six-base) MAPSORT of: pFN21AE5563.seq Check: 4027 from: 1 to: 5941 pFN21AE5563 5941 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2427 3751 278 Size: 53 83 186 936 891 1324 2468 Fragments arranged by size: 2468 1324 936 891 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2044 820 Size: 197 1027 4717 Fragments arranged by size: 4717 1027 197 AflIII A'CryG_T Cuts at: 1203 2896 4840 5817 1203 Size: 1693 1944 977 1327 Fragments arranged by size: 1944 1693 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 3033 1166 Size: 1867 4074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 3233 3684 5256 3233 Size: 451 1572 3918 Fragments arranged by size: 3918 1572 451 ApaI G_GGCC'C Cuts at: 2672 2672 Size: 5941 ApaLI G'TGCA_C Cuts at: 3188 3998 5154 3188 Size: 810 1156 3975 Fragments arranged by size: 3975 1156 810 ApoI r'AATT_y Cuts at: 1493 2397 2456 3182 3250 3476 3628 5737 1493 Size: 904 59 726 68 226 152 2109 1697 Fragments arranged by size: 2109 1697 904 726 226 152 68 59 AseI AT'TA_AT Cuts at: 160 1032 2610 4495 160 Size: 872 1578 1885 1606 Fragments arranged by size: 1885 1606 1578 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 3266 1870 Size: 73 1323 4545 Fragments arranged by size: 4545 1323 73 BamHI G'GATC_C Cuts at: 2485 3271 3638 2485 Size: 786 367 4788 Fragments arranged by size: 4788 786 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3262 4717 618 Size: 325 217 627 1475 1455 1842 Fragments arranged by size: 1842 1475 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2672 3260 1391 Size: 1281 588 4072 Fragments arranged by size: 4072 1281 588 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2724 2994 928 Size: 971 825 270 3875 Fragments arranged by size: 3875 971 825 270 BbvCI CC'TCA_GC Cuts at: 2417 2555 2417 Size: 138 5803 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 2676 2739 2929 1548 Size: 1128 63 190 4560 Fragments arranged by size: 4560 1128 190 63 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2672 2953 3192 4002 5158 1790 Size: 882 281 239 810 1156 2573 Fragments arranged by size: 2573 1156 882 810 281 239 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2925 4601 1239 Size: 1686 1676 2579 Fragments arranged by size: 2579 1686 1676 Bpu10I CC'TnA_GC Cuts at: 2145 2417 2555 2145 Size: 272 138 5531 Fragments arranged by size: 5531 272 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2169 4066 4931 5229 2169 Size: 1897 865 298 2881 Fragments arranged by size: 2881 1897 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2415 4604 882 Size: 617 916 2189 2219 Fragments arranged by size: 2219 2189 916 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2424 3748 4130 4765 Size: 53 83 186 936 891 1324 382 635 Cuts at: 4765 275 Size: 1451 Fragments arranged by size: 1451 1324 936 891 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2430 2460 1178 Size: 30 165 30 1027 30 4659 Fragments arranged by size: 4659 1027 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5941 BseYI C'CCAG_C Cuts at: 2952 5144 5640 2952 Size: 2192 496 3253 Fragments arranged by size: 3253 2192 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3057 1163 Size: 573 1321 4047 Fragments arranged by size: 4047 1321 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2200 2603 3192 4002 4087 5158 2200 Size: 403 589 810 85 1071 2983 Fragments arranged by size: 2983 1071 810 589 403 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2603 2672 2953 3192 3260 4002 Size: 399 410 403 69 281 239 68 742 Cuts at: 4002 4087 5158 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 403 399 281 239 85 69 68 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2376 2655 4436 4610 65 Size: 1183 1128 279 1781 174 1396 Fragments arranged by size: 1781 1396 1183 1128 279 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 3129 794 Size: 2335 3606 BstBI TT'CG_AA Cuts at: 2860 4745 2860 Size: 1885 4056 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2073 2485 3271 3638 4023 4040 5492 5578 Size: 101 412 786 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 786 412 385 367 346 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2354 2685 513 Size: 552 1289 331 3769 Fragments arranged by size: 3769 1289 552 331 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 3028 3040 3457 4251 4271 3028 Size: 12 417 794 20 4698 Fragments arranged by size: 4698 794 417 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2783 3388 4277 Size: 54 1425 50 275 18 953 605 889 Cuts at: 4277 5906 8 Size: 1629 43 Fragments arranged by size: 1629 1425 953 889 605 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2272 3871 1353 Size: 919 1599 3423 Fragments arranged by size: 3423 1599 919 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2925 4004 4601 5388 5656 1239 Size: 393 1293 1079 597 787 268 1524 Fragments arranged by size: 1524 1293 1079 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2152 2668 2669 3014 2152 Size: 516 1 345 5079 Fragments arranged by size: 5079 516 345 1 EcoRI G'AATT_C Cuts at: 2397 2456 3250 3628 2397 Size: 59 794 378 4710 Fragments arranged by size: 4710 794 378 59 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3011 3011 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 5088 1678 Size: 3410 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373 Size: 32 135 32 3010 32 42 32 2626 Fragments arranged by size: 3010 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3086 3285 3531 669 Size: 988 281 1148 199 246 3079 Fragments arranged by size: 3079 1148 988 281 246 199 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2164 2310 5055 5239 1305 Size: 859 146 2745 184 2007 Fragments arranged by size: 2745 2007 859 184 146 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3033 3899 4258 4417 518 Size: 648 1867 866 359 159 2042 Fragments arranged by size: 2042 1867 866 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2416 3091 3357 4034 5182 5427 1606 Size: 810 675 266 677 1148 245 2120 Fragments arranged by size: 2120 1148 810 677 675 266 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5389 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2900 3299 4844 1132 Size: 97 1671 399 1545 2229 Fragments arranged by size: 2229 1671 1545 399 97 PasI CC'CwG_GG Cuts at: 1348 3004 1348 Size: 1656 4285 PciI A'CATG_T Cuts at: 2896 4840 2896 Size: 1944 3997 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 1108 Size: 1026 4915 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2668 2668 Size: 5941 PstI C_TGCA'G Cuts at: 830 2385 3035 3293 830 Size: 1555 650 258 3478 Fragments arranged by size: 3478 1555 650 258 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2416 3091 2416 Size: 675 5266 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2381 2632 2963 3031 3289 4424 5105 Size: 219 1336 251 331 68 258 1135 681 Cuts at: 5105 5296 826 Size: 191 1471 Fragments arranged by size: 1471 1336 1135 681 331 258 251 219 191 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2044 2184 4045 4946 5208 820 Size: 197 926 101 140 1861 901 262 1553 Fragments arranged by size: 1861 1553 926 901 262 197 140 101 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 3380 513 Size: 552 2315 3074 Fragments arranged by size: 3074 2315 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2906 3968 3985 4138 4323 4768 325 Size: 1255 1326 1062 17 153 185 445 1498 Fragments arranged by size: 1498 1326 1255 1062 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4187 5899 96 Size: 274 80 33 51 1443 2210 1712 138 Fragments arranged by size: 2210 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 5901 5933 2430 Size: 32 3439 32 2438 Fragments arranged by size: 3439 2438 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 2031 2651 4070 2031 Size: 620 1419 3902 Fragments arranged by size: 3902 1419 620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2425 3749 276 Size: 53 83 186 936 891 1324 2468 Fragments arranged by size: 2468 1324 936 891 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI