Restriction map for pFN21AE5064
 (Circular) (Six-base) MAPSORT of: pFN21AE5064.seq  Check: 8102  from: 1  to: 6367



pFN21AE5064  6367 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:38  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4177     278 
   Size:         53      83     186     936    2641    2468

  Fragments arranged by size: 

                2641    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3710    1937 
   Size:       1773    4594
 
Acc65I G'GTAC_C

Cuts at:   2023    3688    2023 
   Size:       1665    4702
 
AclI AA'CG_TT

Cuts at:   1563    4494    4867    1563 
   Size:       2931     373    3063

  Fragments arranged by size: 

                3063    2931     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2284    2389    3144    3393    4430    5814    6082    1632 
   Size:        652     105     755     249    1037    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1037     755     652     268     249     105
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6170
 
AflIII A'CryG_T

Cuts at:   1203    3435    5266    6243    1203 
   Size:       2232    1831     977    1327

  Fragments arranged by size: 

                2232    1831    1327     977
 
AgeI A'CCGG_T

Cuts at:   5198    5198 
   Size:       6367
 
AhdI GACnn_n'nnGTC

Cuts at:   2622    5096    2622 
   Size:       2474    3893
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6367
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6367
 
AlwNI CAG_nnn'CTG

Cuts at:   4110    5682    4110 
   Size:       1572    4795
 
ApaLI G'TGCA_C

Cuts at:   2134    2919    4424    5580    2134 
   Size:        785    1505    1156    2921

  Fragments arranged by size: 

                2921    1505    1156     785
 
ApoI r'AATT_y

Cuts at:   1493    2143    2349    2570    3676    3902    4054    6163    1493 
   Size:        650     206     221    1106     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1106     650     226     221     206     152
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4921     160 
   Size:        872    3889    1606

  Fragments arranged by size: 

                3889    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6367
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3692    1870 
   Size:         73    1749    4545

  Fragments arranged by size: 

                4545    1749      73
 
BamHI G'GATC_C

Cuts at:   3697    4064    3697 
   Size:        367    6000
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2023    3688    5143     618 
   Size:        325     217     627     236    1665    1455    1842

  Fragments arranged by size: 

                1842    1665    1455     627     325     236     217
 
BanII G_rGCy'C

Cuts at:   1391    3686    1391 
   Size:       2295    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2649    3071     928 
   Size:        971     750     422    4224

  Fragments arranged by size: 

                4224     971     750     422
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2999    3033    4558    4592    1903 
   Size:         34    1062      34    1525      34    3678

  Fragments arranged by size: 

                3678    1525    1062      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4259    5469    4259 
   Size:       1210    5157
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6367
 
BfrBI ATG'CAT

Cuts at:   2558    2558 
   Size:       6367
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4978     136 
   Size:        107     122      71     631     605    3306    1525

  Fragments arranged by size: 

                3306    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6362    6362 
   Size:       6367
 
BlpI GC'TnA_GC

Cuts at:   3784    3784 
   Size:       6367
 
Bme1580I G_kGCm'C

Cuts at:   1790    2138    2923    4428    5584    1790 
   Size:        348     785    1505    1156    2573

  Fragments arranged by size: 

                2573    1505    1156     785     348
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6367
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3501    4098    5056 
   Size:        416      53      83     399      24    2084     597     958

Cuts at:   5056     442 
   Size:       1753

  Fragments arranged by size: 

                2084    1753     958     597     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6367
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3084    5027    1239 
   Size:       1845    1943    2579

  Fragments arranged by size: 

                2579    1943    1845
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2811    4492    5357    5655    2811 
   Size:       1681     865     298    3523

  Fragments arranged by size: 

                3523    1681     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5030     882 
   Size:        617    3531    2219

  Fragments arranged by size: 

                3531    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3436    4158     493 
   Size:        711    2232     722    2702

  Fragments arranged by size: 

                2702    2232     722     711
 
BsaBI GATnn'nnATC

Cuts at:   6361    6361 
   Size:       6367
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4174    4556    5191     275 
   Size:         53      83     186     936    2641     382     635    1451

  Fragments arranged by size: 

                2641    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4800    5198    5472    5619    6030    4800 
   Size:        398     274     147     411    5137

  Fragments arranged by size: 

                5137     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2117    2147    3499    3529    1178 
   Size:         30     165      30     714      30    1352      30    4016

  Fragments arranged by size: 

                4016    1352     714     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2138    3501    3504    1186 
   Size:        952    1363       3    4049

  Fragments arranged by size: 

                4049    1363     952       3
 
BseYI C'CCAG_C

Cuts at:   5570    6066    5570 
   Size:        496    5871
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3125    1163 
   Size:        573    1389    4405

  Fragments arranged by size: 

                4405    1389     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3817    4578    5606    1540 
   Size:        275     186    1816     761    1028    2301

  Fragments arranged by size: 

                2301    1816    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2138    2923    3355    4428    4513    5584    2138 
   Size:        785     432    1073      85    1071    2921

  Fragments arranged by size: 

                2921    1073    1071     785     432      85
 
BsmI GAATG_Cn'

Cuts at:   3878    3971    3878 
   Size:         93    6274
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2138    2923    3355    3686    4428    4513    5584 
   Size:        399     348     785     432     331     742      85    1071

Cuts at:   5584    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     785     742     432     399     348     331
                  85
 
BspEI T'CCGG_A

Cuts at:   6030    6030 
   Size:       6367
 
BspHI T'CATG_A

Cuts at:   4256    5986    4256 
   Size:       1730    4637
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3722     844 
   Size:       2878    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4254    1394 
   Size:        429    2431    3507

  Fragments arranged by size: 

                3507    2431     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4862    5036      65 
   Size:       1183    3614     174    1396

  Fragments arranged by size: 

                3614    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5011    5198    1700 
   Size:        110    3201     187    2869

  Fragments arranged by size: 

                3201    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6367
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6367
 
BssSI C'ACGA_G

Cuts at:   4427    5439    4427 
   Size:       1012    5355
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6367
 
BstBI TT'CG_AA

Cuts at:   5171    5171 
   Size:       6367
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6367
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2530    2530 
   Size:       6367
 
BstYI r'GATC_y

Cuts at:   1972    3416    3539    3697    4064    4449    4466    5918    6004 
   Size:       1444     123     158     367     385      17    1452      86

Cuts at:   6004    6016    6362    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1444     385     367     346     158     123
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3081    3081 
   Size:       6367
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2544    2958     513 
   Size:        552     939     540     414    3922

  Fragments arranged by size: 

                3922     939     552     540     414
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5628

  Fragments arranged by size: 

                5628     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3883    4677    4697    3883 
   Size:        794      20    5553

  Fragments arranged by size: 

                5553     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6367
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6332
 
DraI TTT'AAA

Cuts at:   3671    3671 
   Size:       6367
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2240    5374     809 
   Size:        507     924    3134    1802

  Fragments arranged by size: 

                3134    1802     924     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3814    4703    6332 
   Size:         54    1425      50     275      18    1984     889    1629

Cuts at:   6332       8 
   Size:         43

  Fragments arranged by size: 

                1984    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3814    1537 
   Size:        275    2002    4090

  Fragments arranged by size: 

                4090    2002     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4297    1353 
   Size:       2944    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2032    4954    5328    5474     387 
   Size:       1064     138     443    2922     374     146    1280

  Fragments arranged by size: 

                2922    1280    1064     443     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2284    2389    3084    3144    3393    4430    5027 
   Size:        393     652     105     695      60     249    1037     597

Cuts at:   5027    5814    6082    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1037     787     695     652     597     393     268
                 249     105      60
 
EcoO109I rG'GnC_Cy

Cuts at:   2210    2624    2210 
   Size:        414    5953
 
EcoRI G'AATT_C

Cuts at:   3676    4054    3676 
   Size:        378    5989
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3258    3258 
   Size:       6367
 
FspI TGC'GCA

Cuts at:   4873    4873 
   Size:       6367
 
HaeII r_GCGC'y

Cuts at:   1678    5514    1678 
   Size:       3836    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2608    2640    2809    2841    5008 
   Size:         32     135      32    1036      32     169      32    2167

Cuts at:   5008    5040    5082    5114    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2167    1036     169     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3025    3711    3957     669 
   Size:        988     281    1087     686     246    3079

  Fragments arranged by size: 

                3079    1087     988     686     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2453     748 
   Size:       1705    4662
 
HpaI GTT'AAC

Cuts at:   3957    3957 
   Size:       6367
 
KpnI G_GTAC'C

Cuts at:   2027    3692    2027 
   Size:       1665    4702
 
MfeI C'AATT_G

Cuts at:   3966    3966 
   Size:       6367
 
MluI A'CGCG_T

Cuts at:   6243    6243 
   Size:       6367
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3394    5481    5665    1305 
   Size:       2089    2087     184    2007

  Fragments arranged by size: 

                2089    2087    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4545

  Fragments arranged by size: 

                4545    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    4325    4684    4843     518 
   Size:        648    3159     359     159    2042

  Fragments arranged by size: 

                3159    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3095    3329    3783    4460    5608    5853    1606 
   Size:       1489     234     454     677    1148     245    2120

  Fragments arranged by size: 

                2120    1489    1148     677     454     245     234
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2544    2958     513 
   Size:        552     939     540     414    3922

  Fragments arranged by size: 

                3922     939     552     540     414
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6367
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6367
 
NotI GC'GGCC_GC

Cuts at:   3814    3814 
   Size:       6367
 
NsiI A_TGCA'T

Cuts at:   2560    2560 
   Size:       6367
 
NspI r_CATG'y

Cuts at:   1132    1229    2562    3725    5270    1132 
   Size:         97    1333    1163    1545    2229

  Fragments arranged by size: 

                2229    1545    1333    1163      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6367
 
PciI A'CATG_T

Cuts at:   5266    5266 
   Size:       6367
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2964    3536    1108 
   Size:       1856     572    3939

  Fragments arranged by size: 

                3939    1856     572
 
PfoI T'CCnGG_A

Cuts at:   2157    2197    2157 
   Size:         40    6327
 
PmeI GTTT'AAAC

Cuts at:   3671    3671 
   Size:       6367
 
PmlI CAC'GTG

Cuts at:   3436    3436 
   Size:       6367
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4436    4468    5949    5981    4436 
   Size:         32    1481      32    4822

  Fragments arranged by size: 

                4822    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2210    2624    2210 
   Size:        414    5953
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6367
 
PsiI TTA'TAA

Cuts at:   3937    3937 
   Size:       6367
 
PstI C_TGCA'G

Cuts at:    830    3138    3346    3456    3719     830 
   Size:       2308     208     110     263    3478

  Fragments arranged by size: 

                3478    2308     263     208     110
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6367
 
PvuII CAG'CTG

Cuts at:   3329    3329 
   Size:       6367
 
SalI G'TCGA_C

Cuts at:   1936    3709    1936 
   Size:       1773    4594
 
SbfI CC_TGCA'GG

Cuts at:   3719    3719 
   Size:       6367
 
ScaI AGT'ACT

Cuts at:   4615    6327    4615 
   Size:       1712    4655
 
SfcI C'TryA_G

Cuts at:    826    1045    3134    3342    3452    3715    4850    5531    5722 
   Size:        219    2089     208     110     263    1135     681     191

Cuts at:   5722     826 
   Size:       1471

  Fragments arranged by size: 

                2089    1471    1135     681     263     219     208     191
                 110
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6367
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6367
 
SmaI CCC'GGG

Cuts at:   1872    3694    1872 
   Size:       1822    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2826    4471    5372    5634     820 
   Size:        197     926     883    1645     901     262    1553

  Fragments arranged by size: 

                1645    1553     926     901     883     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4158     493 
   Size:       3665    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6367
 
SphI G_CATG'C

Cuts at:   1132    2562    3725    1132 
   Size:       1430    1163    3774

  Fragments arranged by size: 

                3774    1430    1163
 
SspI AAT'ATT

Cuts at:      5      52    3040    4291       5 
   Size:         47    2988    1251    2081

  Fragments arranged by size: 

                2988    2081    1251      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6367
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2544    2958    3806     513 
   Size:        552     939     540     414     848    3074

  Fragments arranged by size: 

                3074     939     848     552     540     414
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3270    4394    4411    4564    4749    5194     325 
   Size:       1255    1690    1124      17     153     185     445    1498

  Fragments arranged by size: 

                1690    1498    1255    1124     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4613    6325      96 
   Size:        274      80      33      51    1443    2636    1712     138

  Fragments arranged by size: 

                2636    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2117    2149    6327    6359    2117 
   Size:         32    4178      32    2125

  Fragments arranged by size: 

                4178    2125      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6367
 
XbaI T'CTAG_A

Cuts at:   3703    3703 
   Size:       6367
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2530    3043    2530 
   Size:        513    5854
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6367
 
XmaI C'CCGG_G

Cuts at:   1870    3692    1870 
   Size:       1822    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2856    3229    3406    4496    2856 
   Size:        373     177    1090    4727

  Fragments arranged by size: 

                4727    1090     373     177
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4175     276 
   Size:         53      83     186     936    2641    2468

  Fragments arranged by size: 

                2641    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI
    EcoNI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     PacI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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