Restriction map for
pFN21AB5896
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I CelII CfoI Cfr10I
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1270 GTCGAC GT^[AC][GT]AC
AccII 554 CGCG CG^CG
AflIII 627 ACATGT A^C[GA][CT]GT
AocI 358 CCTCAGG CC^T[ACGT]AGG
AseI 838 ATTAAT AT^TAAT
AsnI 838 ATTAAT AT^TAAT
AspHI 241 GAGCAC G[AT]GC[AT]^C
BalI 1505 TGGCCA TGG^CCA
BanI 1230 GGCACC G^G[CT][GA]CC
BanII 305 GGGCTC G[GA]GC[CT]^C
BbsI 669 GAAGAC GAAGAC(2/6)
BpuAI 669 GAAGAC GAAGAC(2/6)
BsaI 1280 GGTCTC GGTCTC(1/5)
BsaWI 558 ACCGGA [AT]^CCGG[AT]
BsgI 608 CTGCAC GTGCAG(16/14)
BsiEI 556 CGACCG CG[GA][CT]^CG
BsiHKAI 241 GAGCAC G[AT]GC[AT]^C
BsmFI 813 GGGAC GGGAC(10/14)
BsmI 471 GAATGC GAATGC(1/-1)
BspDI 1433 ATCGAT AT^CGAT
BspLU11I 627 ACATGT A^CATGT
BsrDI 1555 GCAATG GCAATG(2/0)
BstEII 727 GGTGACC G^GT[ACGT]ACC
BstPI 727 GGTGACC G^GT[ACGT]ACC
BstUI 554 CGCG CG^CG
BstXI 295 CCAGAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1138 GGATCT [GA]^GATC[CT]
Bsu36I 358 CCTCAGG CC^T[ACGT]AGG
Cac8I 290 GCATGC GC[ACGT]^[ACGT]GC
ClaI 1433 ATCGAT AT^CGAT
DraII 1348 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 1205 CCATGG C^C[GA][CT]GG
Eco81I 358 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1348 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 727 GGTGACC G^GT[ACGT]ACC
EcoRI 280 GAATTC G^AATTC
HapII 559 CCGG C^CGG
HindIII 1253 AAGCTT A^AGCTT
HpaI 919 GTTAAC GTT^AAC
HpaII 559 CCGG C^CGG
MflI 1138 GGATCT [GA]^GATC[CT]
MluNI 1505 TGGCCA TGG^CCA
MscI 1505 TGGCCA TGG^CCA
MspI 559 CCGG C^CGG
MvnI 554 CGCG CG^CG
NcoI 1205 CCATGG C^CATGG
NdeI 1275 CATATG CA^TATG
PleI 391 GACTC GAGTC(4/5)
PshBI 838 ATTAAT AT^TAAT
SalI 1270 GTCGAC G^TCGAC
SexAI 938 ACCTGGT A^CC[AT]GGT
SfcI 316 CTACAG C^T[GA][CT]AG
SmlI 1344 CTTGAG C^T[CT][GA]AG
SphI 290 GCATGC GCATG^C
SspI 1413 AATATT AAT^ATT
XhoII 1138 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 494 CCGC CCGC(-3/-1)
553 CCGC
AcsI 218 AAATTT [GA]^AATT[CT]
280 GAATTC
AhdI 85 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1273 GACATATGGTC
ApoI 218 AAATTT [GA]^AATT[CT]
280 GAATTC
AspEI 85 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1273 GACATATGGTC
BclI 869 TGATCA T^GATCA
1535 TGATCA
BfaI 340 CTAG C^TAG
1520 CTAG
BssKI 146 CCAGG ^CC[ACGT]GG
939 CCTGG
BstAPI 466 GCACTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1027 GCACAGATTGC
BstNI 146 CCAGG CC^[AT]GG
939 CCTGG
Cfr13I 1261 GGCCC G^G[ACGT]CC
1349 GGGCC
EaeI 211 CGGCCA [CT]^GGCC[GA]
1505 TGGCCA
Eam1105I 85 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1273 GACATATGGTC
EcoRII 146 CCAGG ^CC[AT]GG
939 CCTGG
EcoT14I 476 CCAAGG C^C[AT][AT]GG
1205 CCATGG
FauI 493 CCCGC CCCGC(4/6)
552 CCCGC
FbaI 869 TGATCA T^GATCA
1535 TGATCA
HincII 919 GTTAAC GT[CT]^[GA]AC
1270 GTCGAC
HindII 919 GTTAAC GT[CT]^[GA]AC
1270 GTCGAC
MaeI 340 CTAG C^TAG
1520 CTAG
MaeII 803 ACGT A^CGT
1012 ACGT
MvaI 146 CCAGG CC^[AT]GG
939 CCTGG
NlaIV 1230 GGCACC GG[ACGT]^[ACGT]CC
1375 GGAACC
Sau96I 1261 GGCCC G^G[ACGT]CC
1349 GGGCC
ScrFI 146 CCAGG CC^[ACGT]GG
939 CCTGG
SfaNI 265 GATGC GCATC(5/9)
1488 GCATC
StyI 476 CCAAGG C^C[AT][AT]GG
1205 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AlwI BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BstF5I DdeI
DpnII EarI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIII NspI RsaI
Sau3AI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I