(Circular) (Six-base) MAPSORT of: pFN21AB5896.seq Check: 8207 from: 1 to: 6277 pFN21AB5896 6277 bp With 182 enzymes: SgfI * December 3, 2014 10:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3276 3620 1937 Size: 1339 344 4594 Fragments arranged by size: 4594 1339 344 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3377 4340 5724 5992 1632 Size: 1745 963 1384 268 1917 Fragments arranged by size: 1917 1745 1384 963 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 2632 5176 6153 1203 Size: 1429 2544 977 1327 Fragments arranged by size: 2544 1429 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 2095 3283 5006 2095 Size: 1188 1723 3366 Fragments arranged by size: 3366 1723 1188 AleI CACnn'nnGTG Cuts at: 1166 3002 1166 Size: 1836 4441 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 4020 5592 4020 Size: 1572 4705 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2223 2285 3586 3812 3964 6073 1493 Size: 730 62 1301 226 152 2109 1697 Fragments arranged by size: 2109 1697 1301 730 226 152 62 AseI AT'TA_AT Cuts at: 160 1032 2844 4831 160 Size: 872 1812 1987 1606 Fragments arranged by size: 1987 1812 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3602 1870 Size: 73 1659 4545 Fragments arranged by size: 4545 1659 73 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3235 3598 5053 618 Size: 325 217 627 1448 363 1455 1842 Fragments arranged by size: 1842 1455 1448 627 363 325 217 BanII G_rGCy'C Cuts at: 1391 2314 3596 1391 Size: 923 1282 4072 Fragments arranged by size: 4072 1282 923 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2681 928 Size: 971 782 4524 Fragments arranged by size: 4524 971 782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4169 5379 4169 Size: 1210 5067 BclI T'GATC_A Cuts at: 1548 2874 3540 1548 Size: 1326 666 4285 Fragments arranged by size: 4285 1326 666 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4888 136 Size: 107 122 71 631 605 3216 1525 Fragments arranged by size: 3216 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 3178 4338 5494 1790 Size: 1388 1160 1156 2573 Fragments arranged by size: 2573 1388 1160 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2825 4008 4966 Size: 416 53 83 399 24 1408 1183 958 Cuts at: 4966 442 Size: 1753 Fragments arranged by size: 1753 1408 1183 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2448 2448 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2022 2100 2301 4937 1239 Size: 783 78 201 2636 2579 Fragments arranged by size: 2636 2579 783 201 78 Bpu10I CC'TnA_GC Cuts at: 3189 3189 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3370 4402 5267 5565 3370 Size: 1032 865 298 4082 Fragments arranged by size: 4082 1032 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3291 4940 882 Size: 617 1792 1649 2219 Fragments arranged by size: 2219 1792 1649 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2563 4710 5108 5382 5529 5940 2563 Size: 2147 398 274 147 411 2900 Fragments arranged by size: 2900 2147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2527 2557 1178 Size: 30 165 30 1124 30 4898 Fragments arranged by size: 4898 1124 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2220 2424 2528 2531 2532 1186 Size: 1034 204 104 3 1 4931 Fragments arranged by size: 4931 1034 204 104 3 1 BseYI C'CCAG_C Cuts at: 2984 5480 5976 2984 Size: 2496 496 3285 Fragments arranged by size: 3285 2496 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2598 1163 Size: 573 862 4842 Fragments arranged by size: 4842 862 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2564 3727 4488 5516 1540 Size: 275 186 563 1163 761 1028 2301 Fragments arranged by size: 2301 1163 1028 761 563 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 4338 4423 5494 2250 Size: 2088 85 1071 3033 Fragments arranged by size: 3033 2088 1071 85 BsmI GAATG_Cn' Cuts at: 2482 3788 3881 2482 Size: 1306 93 4878 Fragments arranged by size: 4878 1306 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2314 3178 3596 4338 4423 5494 Size: 399 460 64 864 418 742 85 1071 Cuts at: 5494 1391 Size: 2174 Fragments arranged by size: 2174 1071 864 742 460 418 399 85 64 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3632 844 Size: 2788 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4164 1394 Size: 429 2341 3507 Fragments arranged by size: 3507 2341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3567 4772 4946 65 Size: 1183 2319 1205 174 1396 Fragments arranged by size: 2319 1396 1205 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4921 5108 1700 Size: 110 3111 187 2869 Fragments arranged by size: 3111 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 2477 3038 794 Size: 1683 561 4033 Fragments arranged by size: 4033 1683 561 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 2732 1184 Size: 1548 4729 BstXI CCAn_nnnn'nTGG Cuts at: 2307 2307 Size: 6277 BstYI r'GATC_y Cuts at: 1972 3143 3607 3974 4359 4376 5828 5914 5926 Size: 1171 464 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1171 464 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2364 2364 Size: 6277 BtgI C'CryG_G Cuts at: 513 1065 3210 513 Size: 552 2145 3580 Fragments arranged by size: 3580 2145 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 2796 3793 4587 4607 2796 Size: 997 794 20 4466 Fragments arranged by size: 4466 997 794 20 ClaI AT'CG_AT Cuts at: 1554 3439 1554 Size: 1885 4392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2216 3510 3724 Size: 54 1425 50 275 18 386 1294 214 Cuts at: 3724 4613 6242 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1294 889 386 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2526 2688 3560 4207 1353 Size: 780 393 162 872 647 3423 Fragments arranged by size: 3423 872 780 647 393 162 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 2100 2301 3377 4340 4937 5724 Size: 393 390 78 201 1076 963 597 787 Cuts at: 5724 5992 1239 Size: 268 1524 Fragments arranged by size: 1524 1076 963 787 597 393 390 268 201 78 EcoO109I rG'GnC_Cy Cuts at: 3354 3354 Size: 6277 EcoRI G'AATT_C Cuts at: 2285 3586 3964 2285 Size: 1301 378 4598 Fragments arranged by size: 4598 1301 378 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 5424 1678 Size: 3746 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2527 2559 3269 Size: 32 135 32 509 32 414 32 710 Cuts at: 3269 3301 4918 4950 4992 5024 1373 Size: 32 1617 32 42 32 2626 Fragments arranged by size: 2626 1617 710 509 414 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2926 3277 3621 3867 669 Size: 988 281 988 351 344 246 3079 Fragments arranged by size: 3079 988 988 351 344 281 246 HindIII A'AGCT_T Cuts at: 748 3258 748 Size: 2510 3767 HpaI GTT'AAC Cuts at: 2926 3867 2926 Size: 941 5336 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2424 3196 5391 5575 1305 Size: 1119 772 2195 184 2007 Fragments arranged by size: 2195 2007 1119 772 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3512 10 Size: 54 1425 343 1680 2775 Fragments arranged by size: 2775 1680 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 2637 3002 4235 4594 4753 518 Size: 648 1128 343 365 1233 359 159 2042 Fragments arranged by size: 2042 1233 1128 648 365 359 343 159 MspA1I CmG'CkG Cuts at: 1606 3693 4370 5518 5763 1606 Size: 2087 677 1148 245 2120 Fragments arranged by size: 2120 2087 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3210 513 Size: 552 2145 3580 Fragments arranged by size: 3580 2145 552 NdeI CA'TA_TG Cuts at: 387 3281 387 Size: 2894 3383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2299 2636 3086 3635 5180 1132 Size: 97 1070 337 450 549 1545 2229 Fragments arranged by size: 2229 1545 1070 549 450 337 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6277 PciI A'CATG_T Cuts at: 2632 5176 2632 Size: 2544 3733 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PstI C_TGCA'G Cuts at: 830 3629 830 Size: 2799 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3275 3619 1936 Size: 1339 344 4594 Fragments arranged by size: 4594 1339 344 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SexAI A'CCwGG_T Cuts at: 2943 2943 Size: 6277 SfcI C'TryA_G Cuts at: 826 1045 2321 3625 4760 5441 5632 826 Size: 219 1276 1304 1135 681 191 1471 Fragments arranged by size: 1471 1304 1276 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3349 4381 5282 5544 820 Size: 197 926 1406 1032 901 262 1553 Fragments arranged by size: 1553 1406 1032 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 2299 3635 1132 Size: 1167 1336 3774 Fragments arranged by size: 3774 1336 1167 SspI AAT'ATT Cuts at: 5 52 3420 4201 5 Size: 47 3368 781 2081 Fragments arranged by size: 3368 2081 781 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6277 StyI C'CwwG_G Cuts at: 513 1065 2481 3210 3716 513 Size: 552 1416 729 506 3074 Fragments arranged by size: 3074 1416 729 552 506 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4304 4321 4474 4659 5104 325 Size: 1255 2724 17 153 185 445 1498 Fragments arranged by size: 2724 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3124 4523 6235 Size: 274 80 33 51 1443 1147 1399 1712 Cuts at: 6235 96 Size: 138 Fragments arranged by size: 1712 1443 1399 1147 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 6237 Size: 32 6245 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 4406 4406 Size: 6277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI