Restriction map for pFN21AE5571
 (Circular) (Six-base) MAPSORT of: pFN21AE5571.seq  Check: 3778  from: 1  to: 6064



pFN21AE5571  6064 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:23  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3874     278 
   Size:         53      83     186     936    2338    2468

  Fragments arranged by size: 

                2468    2338     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2234    3407    1937 
   Size:        297    1173    4594

  Fragments arranged by size: 

                4594    1173     297
 
Acc65I G'GTAC_C

Cuts at:   3385    3385 
   Size:       6064
 
AclI AA'CG_TT

Cuts at:   1563    4191    4564    1563 
   Size:       2628     373    3063

  Fragments arranged by size: 

                3063    2628     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2193    2395    2978    3134    4127    5511    5779    1632 
   Size:        561     202     583     156     993    1384     268    1917

  Fragments arranged by size: 

                1917    1384     993     583     561     268     202     156
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5867
 
AflIII A'CryG_T

Cuts at:   1203    4963    5940    1203 
   Size:       3760     977    1327

  Fragments arranged by size: 

                3760    1327     977
 
AgeI A'CCGG_T

Cuts at:   4895    4895 
   Size:       6064
 
AhdI GACnn_n'nnGTC

Cuts at:   4793    4793 
   Size:       6064
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6064
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6064
 
AlwNI CAG_nnn'CTG

Cuts at:   3807    5379    3807 
   Size:       1572    4492
 
ApaI G_GGCC'C

Cuts at:   2211    2211 
   Size:       6064
 
ApaLI G'TGCA_C

Cuts at:   4121    5277    4121 
   Size:       1156    4908
 
ApoI r'AATT_y

Cuts at:   1493    3270    3373    3599    3751    5860    1493 
   Size:       1777     103     226     152    2109    1697

  Fragments arranged by size: 

                2109    1777    1697     226     152     103
 
AseI AT'TA_AT

Cuts at:    160    1032    2796    4618     160 
   Size:        872    1764    1822    1606

  Fragments arranged by size: 

                1822    1764    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6064
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3389    1870 
   Size:         73    1446    4545

  Fragments arranged by size: 

                4545    1446      73
 
AvrII C'CTAG_G

Cuts at:   2056    2056 
   Size:       6064
 
BamHI G'GATC_C

Cuts at:   2106    3394    3761    2106 
   Size:       1288     367    4409

  Fragments arranged by size: 

                4409    1288     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3242    3385    4840     618 
   Size:        325     217     627    1455     143    1455    1842

  Fragments arranged by size: 

                1842    1455    1455     627     325     217     143
 
BanII G_rGCy'C

Cuts at:   1391    2211    3383    1391 
   Size:        820    1172    4072

  Fragments arranged by size: 

                4072    1172     820
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2938     928 
   Size:        971    1039    4054

  Fragments arranged by size: 

                4054    1039     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4255    4289    1903 
   Size:         34    2318      34    3678

  Fragments arranged by size: 

                3678    2318      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3956    5166    3956 
   Size:       1210    4854
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6064
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4675     136 
   Size:        107     122      71     631     605    3003    1525

  Fragments arranged by size: 

                3003    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6059    6059 
   Size:       6064
 
BlpI GC'TnA_GC

Cuts at:   3481    3481 
   Size:       6064
 
Bme1580I G_kGCm'C

Cuts at:   1790    2211    2875    4125    5281    1790 
   Size:        421     664    1250    1156    2573

  Fragments arranged by size: 

                2573    1250    1156     664     421
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6064
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3275    3322    3795 
   Size:        416      53      83     399      24    1858      47     473

Cuts at:   3795    4753     442 
   Size:        958    1753

  Fragments arranged by size: 

                1858    1753     958     473     416     399      83      53
                  47      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6064
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2606    4724    1239 
   Size:       1367    2118    2579

  Fragments arranged by size: 

                2579    2118    1367
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2504    4189    5054    5352    2504 
   Size:       1685     865     298    3216

  Fragments arranged by size: 

                3216    1685     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4727     882 
   Size:        617    3228    2219

  Fragments arranged by size: 

                3228    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2565    3855     493 
   Size:        711    1361    1290    2702

  Fragments arranged by size: 

                2702    1361    1290     711
 
BsaBI GATnn'nnATC

Cuts at:   6058    6058 
   Size:       6064
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2009    2342    3871    4253 
   Size:         53      83     186     936     476     333    1529     382

Cuts at:   4253    4888     275 
   Size:        635    1451

  Fragments arranged by size: 

                1529    1451     936     635     476     382     333     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   4497    4895    5169    5316    5727    4497 
   Size:        398     274     147     411    4834

  Fragments arranged by size: 

                4834     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2151    2181    2216    2246    1178 
   Size:         30     165      30     748      30      35      30    4996

  Fragments arranged by size: 

                4996     748     165      35      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6064
 
BseYI C'CCAG_C

Cuts at:   2812    5267    5763    2812 
   Size:       2455     496    3113

  Fragments arranged by size: 

                3113    2455     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5491
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2150    3514    4275    5303    1540 
   Size:        275     186     149    1364     761    1028    2301

  Fragments arranged by size: 

                2301    1364    1028     761     275     186     149
 
BsiHKAI G_wGCw'C

Cuts at:   3177    4125    4210    5281    3177 
   Size:        948      85    1071    3960

  Fragments arranged by size: 

                3960    1071     948      85
 
BsmI GAATG_Cn'

Cuts at:   2939    3575    3668    2939 
   Size:        636      93    5335

  Fragments arranged by size: 

                5335     636      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2019    2019 
   Size:       6064
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2211    2875    3177    3383    4125    4210    5281 
   Size:        399     421     664     302     206     742      85    1071

Cuts at:   5281    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     664     421     399     302     206
                  85
 
BspEI T'CCGG_A

Cuts at:   5727    5727 
   Size:       6064
 
BspHI T'CATG_A

Cuts at:   2733    3953    5683    2733 
   Size:       1220    1730    3114

  Fragments arranged by size: 

                3114    1730    1220
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3419     844 
   Size:       2575    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3951    1394 
   Size:        429    2128    3507

  Fragments arranged by size: 

                3507    2128     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2376    4559    4733      65 
   Size:       1183    1128    2183     174    1396

  Fragments arranged by size: 

                2183    1396    1183    1128     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4708    4895    1700 
   Size:        110    2898     187    2869

  Fragments arranged by size: 

                2898    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6064
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6064
 
BssSI C'ACGA_G

Cuts at:   3178    4124    5136    3178 
   Size:        946    1012    4106

  Fragments arranged by size: 

                4106    1012     946
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2884    3026     794 
   Size:       2090     142    3832

  Fragments arranged by size: 

                3832    2090     142
 
BstBI TT'CG_AA

Cuts at:   4868    4868 
   Size:       6064
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6064
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2482    2911    2482 
   Size:        429    5635
 
BstYI r'GATC_y

Cuts at:   1972    2106    3394    3761    4146    4163    5615    5701    5713 
   Size:        134    1288     367     385      17    1452      86      12

Cuts at:   5713    6059    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1288     385     367     346     134      86
                  17      12
 
BstZ17I GTA'TAC

Cuts at:   2235    2235 
   Size:       6064
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2910     513 
   Size:        552     939     906    3667

  Fragments arranged by size: 

                3667     939     906     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5325

  Fragments arranged by size: 

                5325     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2904    3361    3580    4374    4394    2904 
   Size:        457     219     794      20    4574

  Fragments arranged by size: 

                4574     794     457     219      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6064
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6029
 
DraI TTT'AAA

Cuts at:   3368    3368 
   Size:       6064
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5071     809 
   Size:        507    3755    1802

  Fragments arranged by size: 

                3755    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2147    3511    4400 
   Size:         54    1425      50     275      18     317    1364     889

Cuts at:   4400    6029       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1364     889     317     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2147    3511    1537 
   Size:        275     335    1364    4090

  Fragments arranged by size: 

                4090    1364     335     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2414    3281    3994    1353 
   Size:       1061     867     713    3423

  Fragments arranged by size: 

                3423    1061     867     713
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2243    4651    5025    5171     387 
   Size:       1064     138     654    2408     374     146    1280

  Fragments arranged by size: 

                2408    1280    1064     654     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2193    2395    2606    2978    3134    4127    4724 
   Size:        393     561     202     211     372     156     993     597

Cuts at:   4724    5511    5779    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     993     787     597     561     393     372     268
                 211     202     156
 
EcoRI G'AATT_C

Cuts at:   3270    3373    3751    3270 
   Size:        103     378    5583

  Fragments arranged by size: 

                5583     378     103
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2597    3144    2597 
   Size:        547    5517
 
FspI TGC'GCA

Cuts at:   4570    4570 
   Size:       6064
 
HaeII r_GCGC'y

Cuts at:   1678    3188    5211    1678 
   Size:       1510    2023    2531

  Fragments arranged by size: 

                2531    2023    1510
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2151    2183    2293    2325    2734 
   Size:         32     135      32     579      32     110      32     409

Cuts at:   2734    2766    3115    3147    3174    3206    4705    4737    4779 
   Size:         32     349      32      27      32    1499      32      42

Cuts at:   4779    4811    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1499     579     409     349     135     110      42
                  32      32      32      32      32      32      32      32
                  32      27
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2488    2977    3408    3654     669 
   Size:        988     281     550     489     431     246    3079

  Fragments arranged by size: 

                3079     988     550     489     431     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6064
 
HpaI GTT'AAC

Cuts at:   2488    3654    2488 
   Size:       1166    4898
 
KpnI G_GTAC'C

Cuts at:   3389    3389 
   Size:       6064
 
MfeI C'AATT_G

Cuts at:   3663    3663 
   Size:       6064
 
MluI A'CGCG_T

Cuts at:   5940    5940 
   Size:       6064
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2084    5178    5362    1305 
   Size:        779    3094     184    2007

  Fragments arranged by size: 

                3094    2007     779     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4242

  Fragments arranged by size: 

                4242    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2192    2909    4022    4381    4540     518 
   Size:        648    1026     717    1113     359     159    2042

  Fragments arranged by size: 

                2042    1113    1026     717     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2972    3480    4157    5305    5550    1606 
   Size:       1366     508     677    1148     245    2120

  Fragments arranged by size: 

                2120    1366    1148     677     508     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2910     513 
   Size:        552     939     906    3667

  Fragments arranged by size: 

                3667     939     906     552
 
NdeI CA'TA_TG

Cuts at:    387    2899     387 
   Size:       2512    3552
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6064
 
NotI GC'GGCC_GC

Cuts at:   3511    3511 
   Size:       6064
 
NspI r_CATG'y

Cuts at:   1132    1229    2510    3422    4967    1132 
   Size:         97    1281     912    1545    2229

  Fragments arranged by size: 

                2229    1545    1281     912      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6064
 
PciI A'CATG_T

Cuts at:   4963    4963 
   Size:       6064
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2916    3236    1108 
   Size:       1808     320    3936

  Fragments arranged by size: 

                3936    1808     320
 
PfoI T'CCnGG_A

Cuts at:   2109    2109 
   Size:       6064
 
PmeI GTTT'AAAC

Cuts at:   3368    3368 
   Size:       6064
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4133    4165    5646    5678    4133 
   Size:         32    1481      32    4519

  Fragments arranged by size: 

                4519    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6064
 
PsiI TTA'TAA

Cuts at:   2690    3634    2690 
   Size:        944    5120
 
PspOMI G'GGCC_C

Cuts at:   2207    2207 
   Size:       6064
 
PstI C_TGCA'G

Cuts at:    830    3416     830 
   Size:       2586    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6064
 
PvuII CAG'CTG

Cuts at:   2972    2972 
   Size:       6064
 
SalI G'TCGA_C

Cuts at:   1936    3406    1936 
   Size:       1470    4594
 
SbfI CC_TGCA'GG

Cuts at:   3416    3416 
   Size:       6064
 
ScaI AGT'ACT

Cuts at:   4312    6024    4312 
   Size:       1712    4352
 
SfcI C'TryA_G

Cuts at:    826    1045    3412    4547    5228    5419     826 
   Size:        219    2367    1135     681     191    1471

  Fragments arranged by size: 

                2367    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6064
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6064
 
SmaI CCC'GGG

Cuts at:   1872    3391    1872 
   Size:       1519    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2519    4168    5069    5331     820 
   Size:        197     926     576    1649     901     262    1553

  Fragments arranged by size: 

                1649    1553     926     901     576     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3855     493 
   Size:       3362    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6064
 
SphI G_CATG'C

Cuts at:   1132    2510    3422    1132 
   Size:       1378     912    3774

  Fragments arranged by size: 

                3774    1378     912
 
SspI AAT'ATT

Cuts at:      5      52    3988       5 
   Size:         47    3936    2081

  Fragments arranged by size: 

                3936    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6064
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2056    2910    3503     513 
   Size:        552     939      52     854     593    3074

  Fragments arranged by size: 

                3074     939     854     593     552      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3052    4091    4108    4261    4446    4891     325 
   Size:       1255    1472    1039      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1472    1255    1039     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3309    4310    6022 
   Size:        274      80      33      51    1443    1332    1001    1712

Cuts at:   6022      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1332    1001     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6024    6056    6024 
   Size:         32    6032
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6064
 
XbaI T'CTAG_A

Cuts at:   3400    3400 
   Size:       6064
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3337    3337 
   Size:       6064
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6064
 
XmaI C'CCGG_G

Cuts at:   1870    3389    1870 
   Size:       1519    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4193    4193 
   Size:       6064
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3872     276 
   Size:         53      83     186     936    2338    2468

  Fragments arranged by size: 

                2468    2338     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI    PpuMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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