(Linear) (Six-base) MAPSORT of: KIEE5571.seq Check: 3220 from: 1 to: 1359 KIEE5571.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AccI GT'mk_AC Cuts at: 0 229 1359 Size: 229 1130 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 390 973 1129 1359 Size: 188 202 583 156 230 Fragments arranged by size: 583 230 202 188 156 ApaI G_GGCC'C Cuts at: 0 206 1359 Size: 206 1153 ApoI r'AATT_y Cuts at: 0 1265 1359 Size: 1265 94 AseI AT'TA_AT Cuts at: 0 791 1359 Size: 791 568 AvrII C'CTAG_G Cuts at: 0 51 1359 Size: 51 1308 BamHI G'GATC_C Cuts at: 0 101 1359 Size: 101 1258 BanI G'GyrC_C Cuts at: 0 1237 1359 Size: 1237 122 BanII G_rGCy'C Cuts at: 0 206 1359 Size: 206 1153 BbsI GAAGACnn'nnnn_ Cuts at: 0 933 1359 Size: 933 426 Bme1580I G_kGCm'C Cuts at: 0 206 870 1359 Size: 206 664 489 Fragments arranged by size: 664 489 206 BmrI ACTGGGnnnn_n' Cuts at: 0 1270 1317 1359 Size: 1270 47 42 Fragments arranged by size: 1270 47 42 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 601 1359 Size: 601 758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 1359 Size: 499 860 BsaAI yAC'GTr Cuts at: 0 560 1359 Size: 560 799 BsaHI Gr'CG_yC Cuts at: 0 4 337 1359 Size: 4 333 1022 Fragments arranged by size: 1022 333 4 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 146 176 211 241 1359 Size: 146 30 35 30 1118 Fragments arranged by size: 1118 146 35 30 30 BseYI C'CCAG_C Cuts at: 0 807 1359 Size: 807 552 BsiEI CG_ry'CG Cuts at: 0 145 1359 Size: 145 1214 BsiHKAI G_wGCw'C Cuts at: 0 1172 1359 Size: 1172 187 BsmI GAATG_Cn' Cuts at: 0 934 1359 Size: 934 425 BsmBI CGTCTCn'nnnn_ Cuts at: 0 14 1359 Size: 14 1345 Bsp1286I G_dGCh'C Cuts at: 0 206 870 1172 1359 Size: 206 664 302 187 Fragments arranged by size: 664 302 206 187 BspHI T'CATG_A Cuts at: 0 728 1359 Size: 728 631 BsrDI GCAATG_nn' Cuts at: 0 371 1359 Size: 371 988 BssSI C'ACGA_G Cuts at: 0 1173 1359 Size: 1173 186 BstAPI GCAn_nnn'nTGC Cuts at: 0 879 1021 1359 Size: 879 142 338 Fragments arranged by size: 879 338 142 BstXI CCAn_nnnn'nTGG Cuts at: 0 477 906 1359 Size: 477 429 453 Fragments arranged by size: 477 453 429 BstYI r'GATC_y Cuts at: 0 101 1359 Size: 101 1258 BstZ17I GTA'TAC Cuts at: 0 230 1359 Size: 230 1129 BtgI C'CryG_G Cuts at: 0 905 1359 Size: 905 454 BtsI GCAGTG_nn' Cuts at: 0 899 1356 1359 Size: 899 457 3 Fragments arranged by size: 899 457 3 EaeI y'GGCC_r Cuts at: 0 142 1359 Size: 142 1217 EagI C'GGCC_G Cuts at: 0 142 1359 Size: 142 1217 EarI CTCTTCn'nnn_ Cuts at: 0 409 1276 1359 Size: 409 867 83 Fragments arranged by size: 867 409 83 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 238 1359 Size: 238 1121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 390 601 973 1129 1359 Size: 188 202 211 372 156 230 Fragments arranged by size: 372 230 211 202 188 156 EcoRI G'AATT_C Cuts at: 0 1265 1359 Size: 1265 94 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 592 1139 1359 Size: 592 547 220 Fragments arranged by size: 592 547 220 HaeII r_GCGC'y Cuts at: 0 1183 1359 Size: 1183 176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 146 178 288 320 729 761 1110 1142 Size: 146 32 110 32 409 32 349 32 Cuts at: 1142 1169 1201 1359 Size: 27 32 158 Fragments arranged by size: 409 349 158 146 110 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 0 483 972 1359 Size: 483 489 387 Fragments arranged by size: 489 483 387 HpaI GTT'AAC Cuts at: 0 483 1359 Size: 483 876 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1359 Size: 79 1280 MslI CAynn'nnrTG Cuts at: 0 187 904 1359 Size: 187 717 455 Fragments arranged by size: 717 455 187 MspA1I CmG'CkG Cuts at: 0 967 1359 Size: 967 392 NcoI C'CATG_G Cuts at: 0 905 1359 Size: 905 454 NdeI CA'TA_TG Cuts at: 0 894 1359 Size: 894 465 NspI r_CATG'y Cuts at: 0 505 1359 Size: 505 854 PflMI CCAn_nnn'nTGG Cuts at: 0 911 1231 1359 Size: 911 320 128 Fragments arranged by size: 911 320 128 PfoI T'CCnGG_A Cuts at: 0 104 1359 Size: 104 1255 PsiI TTA'TAA Cuts at: 0 685 1359 Size: 685 674 PspOMI G'GGCC_C Cuts at: 0 202 1359 Size: 202 1157 PvuII CAG'CTG Cuts at: 0 967 1359 Size: 967 392 SmlI C'TyrA_G Cuts at: 0 514 1359 Size: 514 845 SphI G_CATG'C Cuts at: 0 505 1359 Size: 505 854 StyI C'CwwG_G Cuts at: 0 51 905 1359 Size: 51 854 454 Fragments arranged by size: 854 454 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1047 1359 Size: 1047 312 TatI w'GTAC_w Cuts at: 0 1304 1359 Size: 1304 55 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1332 1359 Size: 1332 27 Enzymes that do cut: AccI AcuI ApaI ApoI AseI AvrII BamHI BanI BanII BbsI Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BssSI BstAPI BstXI BstYI BstZ17I BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoRI FalI HaeII Hin4I HincII HpaI MmeI MslI MspA1I NcoI NdeI NspI PflMI PfoI PsiI PspOMI PvuII SmlI SphI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvaI BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaWI BseRI BsgI BsiWI BspEI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI