################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRII EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwNI 282 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 666 GGTCC G^G[AT]CC BbsI 778 GTCTTC GAAGAC(2/6) BbvCI 303 CCTCAGC CCTCAGC(-5/-2) BbvI 627 GCAGC GCAGC(8/12) BcgI 792 CGAAGCTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 119 AGATCT A^GATCT BpmI 159 CTCCAG CTGGAG(16/14) BpuAI 778 GTCTTC GAAGAC(2/6) BsaJI 633 CCATGG C^C[ACGT][ACGT]GG BsmI 802 GCATTC GAATGC(1/-1) BsrDI 511 GCAATG GCAATG(2/0) BstEII 534 GGTAACC G^GT[ACGT]ACC BstPI 534 GGTAACC G^GT[ACGT]ACC Cac8I 291 GCTTGC GC[ACGT]^[ACGT]GC DraII 402 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 633 CCATGG C^C[GA][CT]GG EcoO109I 402 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 534 GGTAACC G^GT[ACGT]ACC EcoRV 576 GATATC GAT^ATC EcoT14I 633 CCATGG C^C[AT][AT]GG Fnu4HI 627 GCAGC GC^[ACGT]GC HaeIII 404 GGCC GG^CC HindIII 677 AAGCTT A^AGCTT ItaI 627 GCAGC GC^[ACGT]GC MslI 732 CATAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 633 CCATGG C^CATGG NlaIV 402 GGGGCC GG[ACGT]^[ACGT]CC NspI 885 GCATGT [GA]CATG^[CT] PflMI 95 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 759 GAGTC GAGTC(4/5) SfaNI 624 GATGC GCATC(5/9) StyI 633 CCATGG C^C[AT][AT]GG TseI 627 GCAGC G^C[AT]GC Tsp45I 691 GTGAC ^GT[GC]AC TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 95 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 29 CCATTCTAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 516 GTAC GT^AC 894 GTAC AlwI 652 GATCC GGATC(4/5) 643 GGATC BfaI 299 CTAG C^TAG 544 CTAG BseRI 366 GAGGAG GAGGAG(10/8) 504 GAGGAG BsiYI 95 CCATTGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCTGATTTGGG BslI 95 CCATTGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCTGATTTGGG BsmAI 111 GAGAC GTCTC(1/5) 157 GTCTC BsrI 287 ACTGG ACTGG(1/-1) 896 ACTGG BstF5I 540 CATCC GGATG(2/0) 831 CATCC Cfr13I 403 GGGCC G^G[ACGT]CC 666 GGTCC FokI 540 CATCC GGATG(9/13) 831 CATCC HphI 390 GGTGA GGTGA(8/7) 900 GGTGA MaeI 299 CTAG C^TAG 544 CTAG RsaI 516 GTAC GT^AC 894 GTAC Sau96I 403 GGGCC G^G[ACGT]CC 666 GGTCC SmlI 322 CTCAAG C^T[CT][GA]AG 647 CTCAAG TfiI 213 GAATC G^A[AT]TC 447 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AcsI AluI ApoI BstYI DdeI DpnII HinfI MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII Sau3AI Tru9I Tsp509I XhoII