Restriction map for pFN21AE5463
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstNI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRII      EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AlwNI         282  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         666  GGTCC                G^G[AT]CC
BbsI          778  GTCTTC               GAAGAC(2/6)
BbvCI         303  CCTCAGC              CCTCAGC(-5/-2)
BbvI          627  GCAGC                GCAGC(8/12)
BcgI          792  CGAAGCTATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         119  AGATCT               A^GATCT
BpmI          159  CTCCAG               CTGGAG(16/14)
BpuAI         778  GTCTTC               GAAGAC(2/6)
BsaJI         633  CCATGG               C^C[ACGT][ACGT]GG
BsmI          802  GCATTC               GAATGC(1/-1)
BsrDI         511  GCAATG               GCAATG(2/0)
BstEII        534  GGTAACC              G^GT[ACGT]ACC
BstPI         534  GGTAACC              G^GT[ACGT]ACC
Cac8I         291  GCTTGC               GC[ACGT]^[ACGT]GC
DraII         402  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          633  CCATGG               C^C[GA][CT]GG
EcoO109I      402  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       534  GGTAACC              G^GT[ACGT]ACC
EcoRV         576  GATATC               GAT^ATC
EcoT14I       633  CCATGG               C^C[AT][AT]GG
Fnu4HI        627  GCAGC                GC^[ACGT]GC
HaeIII        404  GGCC                 GG^CC
HindIII       677  AAGCTT               A^AGCTT
ItaI          627  GCAGC                GC^[ACGT]GC
MslI          732  CATAATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          633  CCATGG               C^CATGG
NlaIV         402  GGGGCC               GG[ACGT]^[ACGT]CC
NspI          885  GCATGT               [GA]CATG^[CT]
PflMI          95  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          759  GAGTC                GAGTC(4/5)
SfaNI         624  GATGC                GCATC(5/9)
StyI          633  CCATGG               C^C[AT][AT]GG
TseI          627  GCAGC                G^C[AT]GC
Tsp45I        691  GTGAC                ^GT[GC]AC
TspRI         144  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I         95  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           29  CCATTCTAATAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          516  GTAC                 GT^AC
              894  GTAC                
AlwI          652  GATCC                GGATC(4/5)
              643  GGATC               
BfaI          299  CTAG                 C^TAG
              544  CTAG                
BseRI         366  GAGGAG               GAGGAG(10/8)
              504  GAGGAG              
BsiYI          95  CCATTGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              834  CCTGATTTGGG         
BslI           95  CCATTGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              834  CCTGATTTGGG         
BsmAI         111  GAGAC                GTCTC(1/5)
              157  GTCTC               
BsrI          287  ACTGG                ACTGG(1/-1)
              896  ACTGG               
BstF5I        540  CATCC                GGATG(2/0)
              831  CATCC               
Cfr13I        403  GGGCC                G^G[ACGT]CC
              666  GGTCC               
FokI          540  CATCC                GGATG(9/13)
              831  CATCC               
HphI          390  GGTGA                GGTGA(8/7)
              900  GGTGA               
MaeI          299  CTAG                 C^TAG
              544  CTAG                
RsaI          516  GTAC                 GT^AC
              894  GTAC                
Sau96I        403  GGGCC                G^G[ACGT]CC
              666  GGTCC               
SmlI          322  CTCAAG               C^T[CT][GA]AG
              647  CTCAAG              
TfiI          213  GAATC                G^A[AT]TC
              447  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AcsI        AluI        ApoI        BstYI       DdeI        
DpnII       HinfI       MaeIII      MboI        MboII       
MflI        MnlI        MseI        NdeII       NlaIII      
Sau3AI      Tru9I       Tsp509I     XhoII       
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