Restriction map for pFN21AE5463
 (Circular) (Six-base) MAPSORT of: pFN21AE5463.seq  Check: 3682  from: 1  to: 5632



pFN21AE5463  5632 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:20  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3442     278 
   Size:         53      83     186     936    1906    2468

  Fragments arranged by size: 

                2468    1906     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2975    1937 
   Size:       1038    4594
 
Acc65I G'GTAC_C

Cuts at:   2953    2953 
   Size:       5632
 
AclI AA'CG_TT

Cuts at:   1563    3759    4132    1563 
   Size:       2196     373    3063

  Fragments arranged by size: 

                3063    2196     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2913    3695    5079    5347    1632 
   Size:       1281     782    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1281     782     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5435
 
AflIII A'CryG_T

Cuts at:   1203    4531    5508    1203 
   Size:       3328     977    1327

  Fragments arranged by size: 

                3328    1327     977
 
AgeI A'CCGG_T

Cuts at:   4463    4463 
   Size:       5632
 
AhdI GACnn_n'nnGTC

Cuts at:   4361    4361 
   Size:       5632
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5632
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5632
 
AlwNI CAG_nnn'CTG

Cuts at:   2292    3375    4947    2292 
   Size:       1083    1572    2977

  Fragments arranged by size: 

                2977    1572    1083
 
ApaLI G'TGCA_C

Cuts at:   3689    4845    3689 
   Size:       1156    4476
 
ApoI r'AATT_y

Cuts at:   1493    2065    2095    2142    2941    3167    3319    5428    1493 
   Size:        572      30      47     799     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     799     572     226     152      47      30
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4186     160 
   Size:        872    3154    1606

  Fragments arranged by size: 

                3154    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5632
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2957    1870 
   Size:         73    1014    4545

  Fragments arranged by size: 

                4545    1014      73
 
BamHI G'GATC_C

Cuts at:   2962    3329    2962 
   Size:        367    5265
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2953    4408     618 
   Size:        325     217     627    1166    1455    1842

  Fragments arranged by size: 

                1842    1455    1166     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2951    1391 
   Size:       1560    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2776     928 
   Size:        971     877    3784

  Fragments arranged by size: 

                3784     971     877
 
BbvCI CC'TCA_GC

Cuts at:   2309    2309 
   Size:       5632
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2786    2820    3823    3857    1903 
   Size:         34     849      34    1003      34    3678

  Fragments arranged by size: 

                3678    1003     849      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3524    4734    3524 
   Size:       1210    4422
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5632
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4243     136 
   Size:        107     122      71     631     605    2571    1525

  Fragments arranged by size: 

                2571    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2124    5627    2124 
   Size:       3503    2129
 
BlpI GC'TnA_GC

Cuts at:   3049    3049 
   Size:       5632
 
Bme1580I G_kGCm'C

Cuts at:   1790    3693    4849    1790 
   Size:       1903    1156    2573

  Fragments arranged by size: 

                2573    1903    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5632
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2911    3363    4321 
   Size:        416      53      83     399      24    1494     452     958

Cuts at:   4321     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1494     958     452     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5632
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2149    4292    1239 
   Size:        910    2143    2579

  Fragments arranged by size: 

                2579    2143     910
 
Bpu10I CC'TnA_GC

Cuts at:   2309    2309 
   Size:       5632
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2312    2637    3757    4622    4920    2312 
   Size:        325    1120     865     298    3024

  Fragments arranged by size: 

                3024    1120     865     325     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4295     882 
   Size:        617    2796    2219

  Fragments arranged by size: 

                2796    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3423     493 
   Size:        711    2219    2702

  Fragments arranged by size: 

                2702    2219     711
 
BsaBI GATnn'nnATC

Cuts at:   5626    5626 
   Size:       5632
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3439    3821    4456     275 
   Size:         53      83     186     936    1906     382     635    1451

  Fragments arranged by size: 

                1906    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4065    4463    4737    4884    5295    4065 
   Size:        398     274     147     411    4402

  Fragments arranged by size: 

                4402     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5407

  Fragments arranged by size: 

                5407     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2386    2524    1186 
   Size:       1200     138    4294

  Fragments arranged by size: 

                4294    1200     138
 
BseYI C'CCAG_C

Cuts at:   2660    4835    5331    2660 
   Size:       2175     496    2961

  Fragments arranged by size: 

                2961    2175     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5059
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3082    3843    4871    1540 
   Size:        275     186    1081     761    1028    2301

  Fragments arranged by size: 

                2301    1081    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3693    3778    4849    3693 
   Size:         85    1071    4476

  Fragments arranged by size: 

                4476    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2807    3143    3236    2807 
   Size:        336      93    5203

  Fragments arranged by size: 

                5203     336      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2951    3693    3778    4849    1391 
   Size:        399    1161     742      85    1071    2174

  Fragments arranged by size: 

                2174    1161    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5295    5295 
   Size:       5632
 
BspHI T'CATG_A

Cuts at:   3521    5251    3521 
   Size:       1730    3902
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2987     844 
   Size:       2143    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3519    1394 
   Size:        429    1696    3507

  Fragments arranged by size: 

                3507    1696     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2523    4127    4301      65 
   Size:       1183    1275    1604     174    1396

  Fragments arranged by size: 

                1604    1396    1275    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4276    4463    1700 
   Size:        110    2466     187    2869

  Fragments arranged by size: 

                2869    2466     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5632
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5632
 
BssSI C'ACGA_G

Cuts at:   3692    4704    3692 
   Size:       1012    4620
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5632
 
BstBI TT'CG_AA

Cuts at:   4436    4436 
   Size:       5632
 
BstEII G'GTnAC_C

Cuts at:   1184    2539    1184 
   Size:       1355    4277
 
BstYI r'GATC_y

Cuts at:   1972    2124    2648    2656    2962    3329    3714    3731    5183 
   Size:        152     524       8     306     367     385      17    1452

Cuts at:   5183    5269    5281    5627    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     524     385     367     346     306     152
                  86      17      12       8
 
BtgI C'CryG_G

Cuts at:    513    1065    2638     513 
   Size:        552    1573    3507

  Fragments arranged by size: 

                3507    1573     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4893

  Fragments arranged by size: 

                4893     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2148    3148    3942    3962    2148 
   Size:       1000     794      20    3818

  Fragments arranged by size: 

                3818    1000     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5632
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5597
 
DraI TTT'AAA

Cuts at:   2936    2936 
   Size:       5632
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4639     809 
   Size:        507    3323    1802

  Fragments arranged by size: 

                3323    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3079    3968    5597 
   Size:         54    1425      50     275      18    1249     889    1629

Cuts at:   5597       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1249     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3079    1537 
   Size:        275    1267    4090

  Fragments arranged by size: 

                4090    1267     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3562    1353 
   Size:       2209    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4219    4593    4739     387 
   Size:       1064     138    2630     374     146    1280

  Fragments arranged by size: 

                2630    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2149    2913    3695    4292    5079    5347    1239 
   Size:        393     517     764     782     597     787     268    1524

  Fragments arranged by size: 

                1524     787     782     764     597     517     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2408    2408 
   Size:       5632
 
EcoRI G'AATT_C

Cuts at:   2941    3319    2941 
   Size:        378    5254
 
EcoRV GAT'ATC

Cuts at:   2583    2583 
   Size:       5632
 
FspI TGC'GCA

Cuts at:   4138    4138 
   Size:       5632
 
HaeII r_GCGC'y

Cuts at:   1678    4779    1678 
   Size:       3101    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2109    2141    4273    4305    4347 
   Size:         32     135      32     537      32    2132      32      42

Cuts at:   4347    4379    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2132     537     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2976    3222     669 
   Size:        988     281    1038     246    3079

  Fragments arranged by size: 

                3079    1038     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2682     748 
   Size:       1934    3698
 
HpaI GTT'AAC

Cuts at:   3222    3222 
   Size:       5632
 
KpnI G_GTAC'C

Cuts at:   2957    2957 
   Size:       5632
 
MfeI C'AATT_G

Cuts at:   3231    3231 
   Size:       5632
 
MluI A'CGCG_T

Cuts at:   5508    5508 
   Size:       5632
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2370    4746    4930    1305 
   Size:       1065    2376     184    2007

  Fragments arranged by size: 

                2376    2007    1065     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3810

  Fragments arranged by size: 

                3810    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2741    3590    3949    4108     518 
   Size:        648    1575     849     359     159    2042

  Fragments arranged by size: 

                2042    1575     849     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3048    3725    4873    5118    1606 
   Size:       1442     677    1148     245    2120

  Fragments arranged by size: 

                2120    1442    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2638     513 
   Size:        552    1573    3507

  Fragments arranged by size: 

                3507    1573     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5632
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5632
 
NotI GC'GGCC_GC

Cuts at:   3079    3079 
   Size:       5632
 
NspI r_CATG'y

Cuts at:   1132    1229    2894    2990    4535    1132 
   Size:         97    1665      96    1545    2229

  Fragments arranged by size: 

                2229    1665    1545      97      96
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5632
 
PciI A'CATG_T

Cuts at:   4531    4531 
   Size:       5632
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2106    1108 
   Size:        998    4634
 
PmeI GTTT'AAAC

Cuts at:   2936    2936 
   Size:       5632
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3701    3733    5214    5246    3701 
   Size:         32    1481      32    4087

  Fragments arranged by size: 

                4087    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5632
 
PsiI TTA'TAA

Cuts at:   3202    3202 
   Size:       5632
 
PstI C_TGCA'G

Cuts at:    830    2984     830 
   Size:       2154    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5632
 
SalI G'TCGA_C

Cuts at:   1936    2974    1936 
   Size:       1038    4594
 
SbfI CC_TGCA'GG

Cuts at:   2984    2984 
   Size:       5632
 
ScaI AGT'ACT

Cuts at:   3880    5592    3880 
   Size:       1712    3920
 
SfcI C'TryA_G

Cuts at:    826    1045    2980    4115    4796    4987     826 
   Size:        219    1935    1135     681     191    1471

  Fragments arranged by size: 

                1935    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5632
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5632
 
SmaI CCC'GGG

Cuts at:   1872    2959    1872 
   Size:       1087    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2327    2652    3736    4637    4899     820 
   Size:        197     926     384     325    1084     901     262    1553

  Fragments arranged by size: 

                1553    1084     926     901     384     325     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3423     493 
   Size:       2930    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5632
 
SphI G_CATG'C

Cuts at:   1132    2990    1132 
   Size:       1858    3774
 
SspI AAT'ATT

Cuts at:      5      52    3556       5 
   Size:         47    3504    2081

  Fragments arranged by size: 

                3504    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5632
 
StyI C'CwwG_G

Cuts at:    513    1065    2638    3071     513 
   Size:        552    1573     433    3074

  Fragments arranged by size: 

                3074    1573     552     433
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2844    2893    3659    3676    3829    4014    4459 
   Size:       1255    1264      49     766      17     153     185     445

Cuts at:   4459     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1264    1255     766     445     185     153      49
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3878    5590      96 
   Size:        274      80      33      51    1443    1901    1712     138

  Fragments arranged by size: 

                1901    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5592    5624    5592 
   Size:         32    5600
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5632
 
XbaI T'CTAG_A

Cuts at:   2968    2968 
   Size:       5632
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2041    2041 
   Size:       5632
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5632
 
XmaI C'CCGG_G

Cuts at:   1870    2957    1870 
   Size:       1087    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3761    3761 
   Size:       5632
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3440     276 
   Size:         53      83     186     936    1906    2468

  Fragments arranged by size: 

                2468    1906     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI
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