################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 412 GCGG CCGC(-3/-1) AcsI 322 AAATTC [GA]^AATT[CT] AlwNI 343 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 322 AAATTC [GA]^AATT[CT] Asp700I 435 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 40 GTGCTC G[AT]GC[AT]^C BfaI 316 CTAG C^TAG BglII 260 AGATCT A^GATCT BlpI 42 GCTCAGC GC^T[ACGT]AGC BmyI 40 GTGCTC G[AGT]GC[ACT]^C BpmI 224 CTCCAG CTGGAG(16/14) Bpu1102I 42 GCTCAGC GC^T[ACGT]AGC BsaI 85 GAGACC GGTCTC(1/5) BsgI 327 CTGCAC GTGCAG(16/14) BsiHKAI 40 GTGCTC G[AT]GC[AT]^C BsiYI 90 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 90 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 85 GAGAC GTCTC(1/5) BsmI 408 GAATGC GAATGC(1/-1) Bsp1286I 40 GTGCTC G[AGT]GC[ACT]^C BspHI 515 TCATGA T^CATGA BsrI 210 CCAGT ACTGG(1/-1) BstF5I 303 GGATG GGATG(2/0) CelII 42 GCTCAGC GC^T[ACGT]AGC FokI 303 GGATG GGATG(9/13) HaeIII 6 GGCC GG^CC MaeI 316 CTAG C^TAG MseI 556 TTAA T^TAA NlaIV 222 GGCTCC GG[ACGT]^[ACGT]CC PflMI 90 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 415 GACTC GAGTC(4/5) RcaI 515 TCATGA T^CATGA Tru9I 556 TTAA T^TAA Tsp509I 323 AATT ^AATT Van91I 90 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 435 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwI 252 GATCC GGATC(4/5) 521 GATCC BsaJI 188 CCCAGG C^C[ACGT][ACGT]GG 491 CCTGGG BseRI 193 GAGGAG GAGGAG(10/8) 379 GAGGAG BstYI 260 AGATCT [GA]^GATC[CT] 520 AGATCC DdeI 43 CTCAG C^T[ACGT]AG 83 CTGAG DraII 109 AGGACCT [GA]G^G[ACGT]CC[CT] 308 AGGACCT EcoO109I 109 AGGACCT [GA]G^G[ACGT]CC[CT] 308 AGGACCT MflI 260 AGATCT [GA]^GATC[CT] 520 AGATCC MspA1I 45 CAGCTG C[AC]G^C[GT]G 346 CAGCTG PpuMI 109 AGGACCT [GA]G^G[AT]CC[CT] 308 AGGACCT PvuII 45 CAGCTG CAG^CTG 346 CAGCTG SfaNI 117 GATGC GCATC(5/9) 372 GATGC XhoII 260 AGATCT [GA]^GATC[CT] 520 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AluI AvaII BbvI BssKI BstNI Cfr13I DpnII EcoRII Fnu4HI HinfI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI TfiI TseI