Restriction map for pFN21AE5366
 (Circular) (Six-base) MAPSORT of: pFN21AE5366.seq  Check: 4675  from: 1  to: 5275



pFN21AE5366  5275 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:18  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3085     278 
   Size:         53      83     186     936    1549    2468

  Fragments arranged by size: 

                2468    1549     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2618    1937 
   Size:        681    4594
 
Acc65I G'GTAC_C

Cuts at:   2596    2596 
   Size:       5275
 
AclI AA'CG_TT

Cuts at:   1563    3402    3775    1563 
   Size:       1839     373    3063

  Fragments arranged by size: 

                3063    1839     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3338    4722    4990    1632 
   Size:       1706    1384     268    1917

  Fragments arranged by size: 

                1917    1706    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5078
 
AflIII A'CryG_T

Cuts at:   1203    4174    5151    1203 
   Size:       2971     977    1327

  Fragments arranged by size: 

                2971    1327     977
 
AgeI A'CCGG_T

Cuts at:   4106    4106 
   Size:       5275
 
AhdI GACnn_n'nnGTC

Cuts at:   4004    4004 
   Size:       5275
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5275
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5275
 
AlwNI CAG_nnn'CTG

Cuts at:   2353    3018    4590    2353 
   Size:        665    1572    3038

  Fragments arranged by size: 

                3038    1572     665
 
ApaLI G'TGCA_C

Cuts at:   3332    4488    3332 
   Size:       1156    4119
 
ApoI r'AATT_y

Cuts at:   1493    2327    2584    2810    2962    5071    1493 
   Size:        834     257     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     834     257     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3829     160 
   Size:        872    2797    1606

  Fragments arranged by size: 

                2797    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5275
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2600    1870 
   Size:         73     657    4545

  Fragments arranged by size: 

                4545     657      73
 
BamHI G'GATC_C

Cuts at:   2605    2972    2605 
   Size:        367    4908
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2596    4051     618 
   Size:        325     217     627     809    1455    1842

  Fragments arranged by size: 

                1842    1455     809     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2594    1391 
   Size:       1203    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4304
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3466    3500    1903 
   Size:         34    1529      34    3678

  Fragments arranged by size: 

                3678    1529      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3167    4377    3167 
   Size:       1210    4065
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5275
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    3886     136 
   Size:        107     122      71     631     605     337    1877    1525

  Fragments arranged by size: 

                1877    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2265    5270    2265 
   Size:       3005    2270
 
BlpI GC'TnA_GC

Cuts at:   2048    2692    2048 
   Size:        644    4631
 
Bme1580I G_kGCm'C

Cuts at:   1790    3336    4492    1790 
   Size:       1546    1156    2573

  Fragments arranged by size: 

                2573    1546    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5275
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3006    3964     442 
   Size:        416      53      83     399      24    1589     958    1753

  Fragments arranged by size: 

                1753    1589     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5275
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2214    3935    1239 
   Size:        975    1721    2579

  Fragments arranged by size: 

                2579    1721     975
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3400    4265    4563    3400 
   Size:        865     298    4112

  Fragments arranged by size: 

                4112     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2084    3938     882 
   Size:        617     585    1854    2219

  Fragments arranged by size: 

                2219    1854     617     585
 
BsaAI yAC'GTr

Cuts at:    493    1204    3066     493 
   Size:        711    1862    2702

  Fragments arranged by size: 

                2702    1862     711
 
BsaBI GATnn'nnATC

Cuts at:   5269    5269 
   Size:       5275
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3082    3464    4099     275 
   Size:         53      83     186     936    1549     382     635    1451

  Fragments arranged by size: 

                1549    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3708    4106    4380    4527    4938    3708 
   Size:        398     274     147     411    4045

  Fragments arranged by size: 

                4045     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5050

  Fragments arranged by size: 

                5050     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2213    2399    1186 
   Size:       1027     186    4062

  Fragments arranged by size: 

                4062    1027     186
 
BseYI C'CCAG_C

Cuts at:   4478    4974    4478 
   Size:        496    4779
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2317    1163 
   Size:        573     581    4121

  Fragments arranged by size: 

                4121     581     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2725    3486    4514    1540 
   Size:        275     186     724     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     724     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2049    3336    3421    4492    2049 
   Size:       1287      85    1071    2832

  Fragments arranged by size: 

                2832    1287    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2419    2786    2879    2419 
   Size:        367      93    4815

  Fragments arranged by size: 

                4815     367      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2049    2594    3336    3421    4492    1391 
   Size:        399     259     545     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     545     399     259      85
 
BspEI T'CCGG_A

Cuts at:   4938    4938 
   Size:       5275
 
BspHI T'CATG_A

Cuts at:   2520    3164    4894    2520 
   Size:        644    1730    2901

  Fragments arranged by size: 

                2901    1730     644
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2630     844 
   Size:       1786    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3162    1394 
   Size:        429    1339    3507

  Fragments arranged by size: 

                3507    1339     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3770    3944      65 
   Size:       1183    2522     174    1396

  Fragments arranged by size: 

                2522    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3919    4106    1700 
   Size:        110    2109     187    2869

  Fragments arranged by size: 

                2869    2109     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5275
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5275
 
BssSI C'ACGA_G

Cuts at:   3335    4347    3335 
   Size:       1012    4263
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5275
 
BstBI TT'CG_AA

Cuts at:   4079    4079 
   Size:       5275
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5275
 
BstYI r'GATC_y

Cuts at:   1972    2265    2525    2605    2972    3357    3374    4826    4912 
   Size:        293     260      80     367     385      17    1452      86

Cuts at:   4912    4924    5270    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     293     260      86
                  80      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4723
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4536

  Fragments arranged by size: 

                4536     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2791    3585    3605    2791 
   Size:        794      20    4461

  Fragments arranged by size: 

                4461     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5275
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5240
 
DraI TTT'AAA

Cuts at:   2579    2579 
   Size:       5275
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4282     809 
   Size:        507    2966    1802

  Fragments arranged by size: 

                2966    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2722    3611    5240 
   Size:         54    1425      50     275      18     892     889    1629

Cuts at:   5240       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     892     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2722    1537 
   Size:        275     910    4090

  Fragments arranged by size: 

                4090     910     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3205    1353 
   Size:       1852    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3862    4236    4382     387 
   Size:       1064     138    2273     374     146    1280

  Fragments arranged by size: 

                2273    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2214    3338    3935    4722    4990    1239 
   Size:        393     582    1124     597     787     268    1524

  Fragments arranged by size: 

                1524    1124     787     597     582     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2115    2314    2115 
   Size:        199    5076
 
EcoRI G'AATT_C

Cuts at:   2584    2962    2584 
   Size:        378    4897
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2461    2461 
   Size:       5275
 
FspI TGC'GCA

Cuts at:   3781    3781 
   Size:       5275
 
HaeII r_GCGC'y

Cuts at:   1678    4422    1678 
   Size:       2744    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2502    2534    3916    3948    3990 
   Size:         32     135      32     930      32    1382      32      42

Cuts at:   3990    4022    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1382     930     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2619    2865     669 
   Size:        988     281     681     246    3079

  Fragments arranged by size: 

                3079     988     681     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5275
 
HpaI GTT'AAC

Cuts at:   2865    2865 
   Size:       5275
 
KpnI G_GTAC'C

Cuts at:   2600    2600 
   Size:       5275
 
MfeI C'AATT_G

Cuts at:   2874    2874 
   Size:       5275
 
MluI A'CGCG_T

Cuts at:   5151    5151 
   Size:       5275
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4389    4573    1305 
   Size:       3084     184    2007

  Fragments arranged by size: 

                3084    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3453

  Fragments arranged by size: 

                3453    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3233    3592    3751     518 
   Size:        648    2067     359     159    2042

  Fragments arranged by size: 

                2067    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2052    2353    2691    3368    4516    4761    1606 
   Size:        446     301     338     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     446     338     301     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4723
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5275
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5275
 
NotI GC'GGCC_GC

Cuts at:   2722    2722 
   Size:       5275
 
NspI r_CATG'y

Cuts at:   1132    1229    2633    4178    1132 
   Size:         97    1404    1545    2229

  Fragments arranged by size: 

                2229    1545    1404      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5275
 
PciI A'CATG_T

Cuts at:   4174    4174 
   Size:       5275
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2101    1108 
   Size:        993    4282
 
PmeI GTTT'AAAC

Cuts at:   2579    2579 
   Size:       5275
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3344    3376    4857    4889    3344 
   Size:         32    1481      32    3730

  Fragments arranged by size: 

                3730    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2115    2314    2115 
   Size:        199    5076
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5275
 
PsiI TTA'TAA

Cuts at:   2845    2845 
   Size:       5275
 
PstI C_TGCA'G

Cuts at:    830    2627     830 
   Size:       1797    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5275
 
PvuII CAG'CTG

Cuts at:   2052    2353    2052 
   Size:        301    4974
 
SalI G'TCGA_C

Cuts at:   1936    2617    1936 
   Size:        681    4594
 
SbfI CC_TGCA'GG

Cuts at:   2627    2627 
   Size:       5275
 
ScaI AGT'ACT

Cuts at:   3523    5235    3523 
   Size:       1712    3563
 
SfcI C'TryA_G

Cuts at:    826    1045    2623    3758    4439    4630     826 
   Size:        219    1578    1135     681     191    1471

  Fragments arranged by size: 

                1578    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5275
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5275
 
SmaI CCC'GGG

Cuts at:   1872    2602    1872 
   Size:        730    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3379    4280    4542     820 
   Size:        197     926    1436     901     262    1553

  Fragments arranged by size: 

                1553    1436     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3066     493 
   Size:       2573    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5275
 
SphI G_CATG'C

Cuts at:   1132    2633    1132 
   Size:       1501    3774
 
SspI AAT'ATT

Cuts at:      5      52    3199       5 
   Size:         47    3147    2081

  Fragments arranged by size: 

                3147    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5275
 
StyI C'CwwG_G

Cuts at:    513    1065    2714     513 
   Size:        552    1649    3074

  Fragments arranged by size: 

                3074    1649     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3302    3319    3472    3657    4102     325 
   Size:       1255    1722      17     153     185     445    1498

  Fragments arranged by size: 

                1722    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3521    5233      96 
   Size:        274      80      33      51    1443    1544    1712     138

  Fragments arranged by size: 

                1712    1544    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5235    5267    5235 
   Size:         32    5243
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5275
 
XbaI T'CTAG_A

Cuts at:   2611    2611 
   Size:       5275
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5275
 
XmaI C'CCGG_G

Cuts at:   1870    2600    1870 
   Size:        730    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2444    3404    2444 
   Size:        960    4315
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3083     276 
   Size:         53      83     186     936    1549    2468

  Fragments arranged by size: 

                2468    1549     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI    EcoRV     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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