Restriction map for pFN21AE5333
 (Linear) (Six-base) MAPSORT of: KIEE5333.seq  Check: 1678  from: 1  to: 825



KIEE5333.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     809     825 
   Size:        809      16
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     825 
   Size:        301     524
 
AfeI AGC'GCT

Cuts at:      0     607     825 
   Size:        607     218
 
AflIII A'CryG_T

Cuts at:      0     746     825 
   Size:        746      79
 
AlwNI CAG_nnn'CTG

Cuts at:      0     668     693     825 
   Size:        668      25     132

  Fragments arranged by size: 

                 668     132      25
 
AvaI C'yCGr_G

Cuts at:      0     681     700     825 
   Size:        681      19     125

  Fragments arranged by size: 

                 681     125      19
 
BanI G'GyrC_C

Cuts at:      0     710     825 
   Size:        710     115
 
BanII G_rGCy'C

Cuts at:      0     727     825 
   Size:        727      98
 
BbeI G_GCGC'C

Cuts at:      0     714     825 
   Size:        714     111
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     289     825 
   Size:        289     536
 
BlpI GC'TnA_GC

Cuts at:      0      34     825 
   Size:         34     791
 
BmgBI CAC'GTC

Cuts at:      0     747     825 
   Size:        747      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     723     825 
   Size:        444     279     102

  Fragments arranged by size: 

                 444     279     102
 
BsaHI Gr'CG_yC

Cuts at:      0     711     825 
   Size:        711     114
 
BsaWI w'CCGG_w

Cuts at:      0     240     825 
   Size:        240     585
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     610     640     825 
   Size:        610      30     185

  Fragments arranged by size: 

                 610     185      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     631     634     825 
   Size:        631       3     191

  Fragments arranged by size: 

                 631     191       3
 
BseYI C'CCAG_C

Cuts at:      0     490     825 
   Size:        490     335
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     787     825 
   Size:        145     642      38

  Fragments arranged by size: 

                 642     145      38
 
BsiEI CG_ry'CG

Cuts at:      0     720     825 
   Size:        720     105
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     664     825 
   Size:        664     161
 
Bsp1286I G_dGCh'C

Cuts at:      0     727     825 
   Size:        727      98
 
BspHI T'CATG_A

Cuts at:      0     479     825 
   Size:        479     346
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     184     809     825 
   Size:        184     625      16

  Fragments arranged by size: 

                 625     184      16
 
BsrFI r'CCGG_y

Cuts at:      0     166     574     825 
   Size:        166     408     251

  Fragments arranged by size: 

                 408     251     166
 
BsrGI T'GTAC_A

Cuts at:      0     101     825 
   Size:        101     724
 
BssSI C'ACGA_G

Cuts at:      0     328     825 
   Size:        328     497
 
BtgI C'CryG_G

Cuts at:      0     311     714     825 
   Size:        311     403     111

  Fragments arranged by size: 

                 403     311     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      60     825 
   Size:         60     765
 
BtsI GCAGTG_nn'

Cuts at:      0       6     825 
   Size:          6     819
 
EaeI y'GGCC_r

Cuts at:      0     296     360     717     825 
   Size:        296      64     357     108

  Fragments arranged by size: 

                 357     296     108      64
 
EagI C'GGCC_G

Cuts at:      0     717     825 
   Size:        717     108
 
EarI CTCTTCn'nnn_

Cuts at:      0     328     825 
   Size:        328     497
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     358     825 
   Size:        358     467
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     444     723     825 
   Size:        301     143     279     102

  Fragments arranged by size: 

                 301     279     143     102
 
FspI TGC'GCA

Cuts at:      0     292     825 
   Size:        292     533
 
FspAI rTGC'GCAy

Cuts at:      0     292     825 
   Size:        292     533
 
HaeII r_GCGC'y

Cuts at:      0     609     714     825 
   Size:        609     105     111

  Fragments arranged by size: 

                 609     111     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     615     647     825 
   Size:        615      32     178

  Fragments arranged by size: 

                 615     178      32
 
KasI G'GCGC_C

Cuts at:      0     710     825 
   Size:        710     115
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     698     755     825 
   Size:        698      57      70

  Fragments arranged by size: 

                 698      70      57
 
MslI CAynn'nnrTG

Cuts at:      0     757     825 
   Size:        757      68
 
MspA1I CmG'CkG

Cuts at:      0     716     825 
   Size:        716     109
 
NaeI GCC'GGC

Cuts at:      0     168     576     825 
   Size:        168     408     249

  Fragments arranged by size: 

                 408     249     168
 
NarI GG'CG_CC

Cuts at:      0     711     825 
   Size:        711     114
 
NcoI C'CATG_G

Cuts at:      0     311     825 
   Size:        311     514
 
NgoMIV G'CCGG_C

Cuts at:      0     166     574     825 
   Size:        166     408     251

  Fragments arranged by size: 

                 408     251     166
 
PstI C_TGCA'G

Cuts at:      0      13     698     825 
   Size:         13     685     127

  Fragments arranged by size: 

                 685     127      13
 
SacII CC_GC'GG

Cuts at:      0     717     825 
   Size:        717     108
 
SfcI C'TryA_G

Cuts at:      0       9     694     825 
   Size:          9     685     131

  Fragments arranged by size: 

                 685     131       9
 
SfoI GGC'GCC

Cuts at:      0     712     825 
   Size:        712     113
 
SspI AAT'ATT

Cuts at:      0     229     276     825 
   Size:        229      47     549

  Fragments arranged by size: 

                 549     229      47
 
StyI C'CwwG_G

Cuts at:      0     311     431     825 
   Size:        311     120     394

  Fragments arranged by size: 

                 394     311     120
 
TatI w'GTAC_w

Cuts at:      0     101     825 
   Size:        101     724
 
Tth111I GACn'n_nGTC

Cuts at:      0     475     825 
   Size:        475     350
 
XmnI GAAnn'nnTTC

Cuts at:      0     230     825 
   Size:        230     595
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII    AlwNI     AvaI     BanI    BanII
     BbeI     BbsI     BlpI    BmgBI     BpmI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I    BspHI    BspMI
    BsrFI    BsrGI    BssSI     BtgI    BtgZI     BtsI     EaeI     EagI
     EarI     EciI  Eco57MI     FspI    FspAI    HaeII    Hin4I     KasI
     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PstI
    SacII     SfcI     SfoI     SspI     StyI     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI  BsiHKAI    BsiWI     BsmI    BspEI    BsrBI
    BsrDI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI
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