(Linear) (Six-base) MAPSORT of: KIEE5333.seq Check: 1678 from: 1 to: 825 KIEE5333.orf With 182 enzymes: SgfI * January 29, 2014 11:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 809 825 Size: 809 16 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 825 Size: 301 524 AfeI AGC'GCT Cuts at: 0 607 825 Size: 607 218 AflIII A'CryG_T Cuts at: 0 746 825 Size: 746 79 AlwNI CAG_nnn'CTG Cuts at: 0 668 693 825 Size: 668 25 132 Fragments arranged by size: 668 132 25 AvaI C'yCGr_G Cuts at: 0 681 700 825 Size: 681 19 125 Fragments arranged by size: 681 125 19 BanI G'GyrC_C Cuts at: 0 710 825 Size: 710 115 BanII G_rGCy'C Cuts at: 0 727 825 Size: 727 98 BbeI G_GCGC'C Cuts at: 0 714 825 Size: 714 111 BbsI GAAGACnn'nnnn_ Cuts at: 0 289 825 Size: 289 536 BlpI GC'TnA_GC Cuts at: 0 34 825 Size: 34 791 BmgBI CAC'GTC Cuts at: 0 747 825 Size: 747 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 444 723 825 Size: 444 279 102 Fragments arranged by size: 444 279 102 BsaHI Gr'CG_yC Cuts at: 0 711 825 Size: 711 114 BsaWI w'CCGG_w Cuts at: 0 240 825 Size: 240 585 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 610 640 825 Size: 610 30 185 Fragments arranged by size: 610 185 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 631 634 825 Size: 631 3 191 Fragments arranged by size: 631 191 3 BseYI C'CCAG_C Cuts at: 0 490 825 Size: 490 335 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 787 825 Size: 145 642 38 Fragments arranged by size: 642 145 38 BsiEI CG_ry'CG Cuts at: 0 720 825 Size: 720 105 BsmBI CGTCTCn'nnnn_ Cuts at: 0 664 825 Size: 664 161 Bsp1286I G_dGCh'C Cuts at: 0 727 825 Size: 727 98 BspHI T'CATG_A Cuts at: 0 479 825 Size: 479 346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 184 809 825 Size: 184 625 16 Fragments arranged by size: 625 184 16 BsrFI r'CCGG_y Cuts at: 0 166 574 825 Size: 166 408 251 Fragments arranged by size: 408 251 166 BsrGI T'GTAC_A Cuts at: 0 101 825 Size: 101 724 BssSI C'ACGA_G Cuts at: 0 328 825 Size: 328 497 BtgI C'CryG_G Cuts at: 0 311 714 825 Size: 311 403 111 Fragments arranged by size: 403 311 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 60 825 Size: 60 765 BtsI GCAGTG_nn' Cuts at: 0 6 825 Size: 6 819 EaeI y'GGCC_r Cuts at: 0 296 360 717 825 Size: 296 64 357 108 Fragments arranged by size: 357 296 108 64 EagI C'GGCC_G Cuts at: 0 717 825 Size: 717 108 EarI CTCTTCn'nnn_ Cuts at: 0 328 825 Size: 328 497 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 358 825 Size: 358 467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 444 723 825 Size: 301 143 279 102 Fragments arranged by size: 301 279 143 102 FspI TGC'GCA Cuts at: 0 292 825 Size: 292 533 FspAI rTGC'GCAy Cuts at: 0 292 825 Size: 292 533 HaeII r_GCGC'y Cuts at: 0 609 714 825 Size: 609 105 111 Fragments arranged by size: 609 111 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 615 647 825 Size: 615 32 178 Fragments arranged by size: 615 178 32 KasI G'GCGC_C Cuts at: 0 710 825 Size: 710 115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 698 755 825 Size: 698 57 70 Fragments arranged by size: 698 70 57 MslI CAynn'nnrTG Cuts at: 0 757 825 Size: 757 68 MspA1I CmG'CkG Cuts at: 0 716 825 Size: 716 109 NaeI GCC'GGC Cuts at: 0 168 576 825 Size: 168 408 249 Fragments arranged by size: 408 249 168 NarI GG'CG_CC Cuts at: 0 711 825 Size: 711 114 NcoI C'CATG_G Cuts at: 0 311 825 Size: 311 514 NgoMIV G'CCGG_C Cuts at: 0 166 574 825 Size: 166 408 251 Fragments arranged by size: 408 251 166 PstI C_TGCA'G Cuts at: 0 13 698 825 Size: 13 685 127 Fragments arranged by size: 685 127 13 SacII CC_GC'GG Cuts at: 0 717 825 Size: 717 108 SfcI C'TryA_G Cuts at: 0 9 694 825 Size: 9 685 131 Fragments arranged by size: 685 131 9 SfoI GGC'GCC Cuts at: 0 712 825 Size: 712 113 SspI AAT'ATT Cuts at: 0 229 276 825 Size: 229 47 549 Fragments arranged by size: 549 229 47 StyI C'CwwG_G Cuts at: 0 311 431 825 Size: 311 120 394 Fragments arranged by size: 394 311 120 TatI w'GTAC_w Cuts at: 0 101 825 Size: 101 724 Tth111I GACn'n_nGTC Cuts at: 0 475 825 Size: 475 350 XmnI GAAnn'nnTTC Cuts at: 0 230 825 Size: 230 595 Enzymes that do cut: AarI AcuI AfeI AflIII AlwNI AvaI BanI BanII BbeI BbsI BlpI BmgBI BpmI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI FspI FspAI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PstI SacII SfcI SfoI SspI StyI TatI Tth111I XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsiHKAI BsiWI BsmI BspEI BsrBI BsrDI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI ZraI