(Circular) (Six-base) MAPSORT of: pFN21AE5136.seq Check: 6286 from: 1 to: 5113 pFN21AE5136 5113 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2923 278 Size: 53 83 186 936 1387 2468 Fragments arranged by size: 2468 1387 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2036 2456 1937 Size: 99 420 4594 Fragments arranged by size: 4594 420 99 Acc65I G'GTAC_C Cuts at: 2434 2434 Size: 5113 AclI AA'CG_TT Cuts at: 1563 3240 3613 1563 Size: 1677 373 3063 Fragments arranged by size: 3063 1677 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3176 4560 4828 1632 Size: 1544 1384 268 1917 Fragments arranged by size: 1917 1544 1384 268 AfeI AGC'GCT Cuts at: 2092 2092 Size: 5113 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4916 AflIII A'CryG_T Cuts at: 1203 4012 4989 1203 Size: 2809 977 1327 Fragments arranged by size: 2809 1327 977 AgeI A'CCGG_T Cuts at: 3944 3944 Size: 5113 AhdI GACnn_n'nnGTC Cuts at: 3842 3842 Size: 5113 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5113 AlwNI CAG_nnn'CTG Cuts at: 2215 2856 4428 2215 Size: 641 1572 2900 Fragments arranged by size: 2900 1572 641 ApaLI G'TGCA_C Cuts at: 3170 4326 3170 Size: 1156 3957 ApoI r'AATT_y Cuts at: 1493 2422 2648 2800 4909 1493 Size: 929 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 226 152 AseI AT'TA_AT Cuts at: 160 1032 3667 160 Size: 872 2635 1606 Fragments arranged by size: 2635 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5113 AvaI C'yCGr_G Cuts at: 1870 1943 2438 1870 Size: 73 495 4545 Fragments arranged by size: 4545 495 73 BamHI G'GATC_C Cuts at: 2443 2810 2443 Size: 367 4746 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2138 2434 3889 618 Size: 325 217 627 263 88 296 1455 1842 Fragments arranged by size: 1842 1455 627 325 296 263 217 88 BanII G_rGCy'C Cuts at: 1391 2267 2432 1391 Size: 876 165 4072 Fragments arranged by size: 4072 876 165 BbeI G_GCGC'C Cuts at: 2054 2054 Size: 5113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2009 2043 3304 3338 1903 Size: 34 72 34 1261 34 3678 Fragments arranged by size: 3678 1261 72 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3005 4215 3005 Size: 1210 3903 BclI T'GATC_A Cuts at: 1548 1548 Size: 5113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 3724 136 Size: 107 122 71 631 605 403 1649 1525 Fragments arranged by size: 1649 1525 631 605 403 122 107 71 BglII A'GATC_T Cuts at: 5108 5108 Size: 5113 BlpI GC'TnA_GC Cuts at: 2530 2530 Size: 5113 Bme1580I G_kGCm'C Cuts at: 1790 2141 3174 4330 1790 Size: 351 1033 1156 2573 Fragments arranged by size: 2573 1156 1033 351 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5113 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2844 3802 442 Size: 416 53 83 399 24 1427 958 1753 Fragments arranged by size: 1753 1427 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3773 1239 Size: 2534 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3238 4103 4401 3238 Size: 865 298 3950 Fragments arranged by size: 3950 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3776 882 Size: 617 2277 2219 Fragments arranged by size: 2277 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2904 493 Size: 711 1700 2702 Fragments arranged by size: 2702 1700 711 BsaBI GATnn'nnATC Cuts at: 5107 5107 Size: 5113 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2051 2920 3302 3937 Size: 53 83 186 936 518 869 382 635 Cuts at: 3937 275 Size: 1451 Fragments arranged by size: 1451 936 869 635 518 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3546 3944 4218 4365 4776 3546 Size: 398 274 147 411 3883 Fragments arranged by size: 3883 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4888 Fragments arranged by size: 4888 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5113 BseYI C'CCAG_C Cuts at: 4316 4812 4316 Size: 496 4617 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 2563 3324 4352 1540 Size: 275 186 134 428 761 1028 2301 Fragments arranged by size: 2301 1028 761 428 275 186 134 BsiHKAI G_wGCw'C Cuts at: 2267 3174 3259 4330 2267 Size: 907 85 1071 3050 Fragments arranged by size: 3050 1071 907 85 BsmI GAATG_Cn' Cuts at: 2624 2717 2624 Size: 93 5020 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2267 2432 3174 3259 4330 1391 Size: 399 351 126 165 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 351 165 126 85 BspEI T'CCGG_A Cuts at: 4776 4776 Size: 5113 BspHI T'CATG_A Cuts at: 3002 4732 3002 Size: 1730 3383 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2468 844 Size: 1415 209 3489 Fragments arranged by size: 3489 1415 209 BsrBI CCG'CTC Cuts at: 1394 1823 3000 1394 Size: 429 1177 3507 Fragments arranged by size: 3507 1177 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3608 3782 65 Size: 1183 2360 174 1396 Fragments arranged by size: 2360 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 2130 3757 3944 1700 Size: 110 292 28 1627 187 2869 Fragments arranged by size: 2869 1627 292 187 110 28 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5113 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5113 BssSI C'ACGA_G Cuts at: 3173 4185 3173 Size: 1012 4101 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5113 BstBI TT'CG_AA Cuts at: 3917 3917 Size: 5113 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5113 BstYI r'GATC_y Cuts at: 1972 2157 2443 2810 3195 3212 4664 4750 4762 Size: 185 286 367 385 17 1452 86 12 Cuts at: 4762 5108 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 286 185 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 2398 513 Size: 552 939 138 256 3228 Fragments arranged by size: 3228 939 552 256 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4374 Fragments arranged by size: 4374 683 56 BtsI GCAGTG_nn' Cuts at: 2629 3423 3443 2629 Size: 794 20 4299 Fragments arranged by size: 4299 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5113 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5078 DraI TTT'AAA Cuts at: 2242 2417 2242 Size: 175 4938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4120 809 Size: 507 2804 1802 Fragments arranged by size: 2804 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2097 2132 Size: 54 1425 50 275 18 237 30 35 Cuts at: 2132 2560 3449 5078 8 Size: 428 889 1629 43 Fragments arranged by size: 1629 1425 889 428 275 237 54 50 43 35 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2132 2560 1537 Size: 275 320 428 4090 Fragments arranged by size: 4090 428 320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3043 1353 Size: 1690 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2150 3700 4074 4220 387 Size: 1064 138 561 1550 374 146 1280 Fragments arranged by size: 1550 1280 1064 561 374 146 138 EcoICRI GAG'CTC Cuts at: 2265 2265 Size: 5113 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3176 3773 4560 4828 1239 Size: 393 1544 597 787 268 1524 Fragments arranged by size: 1544 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2422 2800 2422 Size: 378 4735 FspI TGC'GCA Cuts at: 2232 3619 2232 Size: 1387 3726 HaeII r_GCGC'y Cuts at: 1678 2054 2094 2215 4260 1678 Size: 376 40 121 2045 2531 Fragments arranged by size: 2531 2045 376 121 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3754 3786 3828 3860 1373 Size: 32 135 32 2182 32 42 32 2626 Fragments arranged by size: 2626 2182 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2457 2703 669 Size: 988 281 519 246 3079 Fragments arranged by size: 3079 988 519 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5113 HpaI GTT'AAC Cuts at: 2703 2703 Size: 5113 KasI G'GCGC_C Cuts at: 2050 2050 Size: 5113 KpnI G_GTAC'C Cuts at: 2438 2438 Size: 5113 MfeI C'AATT_G Cuts at: 2712 2712 Size: 5113 MluI A'CGCG_T Cuts at: 4989 4989 Size: 5113 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4227 4411 1305 Size: 2922 184 2007 Fragments arranged by size: 2922 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 10 Size: 54 1425 343 237 3054 Fragments arranged by size: 3054 1425 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 3071 3430 3589 518 Size: 648 1197 708 359 159 2042 Fragments arranged by size: 2042 1197 708 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2270 2400 2529 3206 4354 4599 1606 Size: 664 130 129 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 245 130 129 NarI GG'CG_CC Cuts at: 2051 2051 Size: 5113 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3622 Fragments arranged by size: 3622 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5113 NotI GC'GGCC_GC Cuts at: 2560 2560 Size: 5113 NspI r_CATG'y Cuts at: 1132 1229 2471 4016 1132 Size: 97 1242 1545 2229 Fragments arranged by size: 2229 1545 1242 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5113 PciI A'CATG_T Cuts at: 4012 4012 Size: 5113 PflMI CCAn_nnn'nTGG Cuts at: 1108 2215 1108 Size: 1107 4006 PmeI GTTT'AAAC Cuts at: 2417 2417 Size: 5113 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3182 3214 4695 4727 3182 Size: 32 1481 32 3568 Fragments arranged by size: 3568 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5113 PsiI TTA'TAA Cuts at: 2683 2683 Size: 5113 PstI C_TGCA'G Cuts at: 830 2286 2465 830 Size: 1456 179 3478 Fragments arranged by size: 3478 1456 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5113 SacI G_AGCT'C Cuts at: 2267 2267 Size: 5113 SacII CC_GC'GG Cuts at: 2401 2401 Size: 5113 SalI G'TCGA_C Cuts at: 1936 2455 1936 Size: 519 4594 SbfI CC_TGCA'GG Cuts at: 2465 2465 Size: 5113 ScaI AGT'ACT Cuts at: 3361 5073 3361 Size: 1712 3401 SfcI C'TryA_G Cuts at: 826 1045 2282 2461 3596 4277 4468 826 Size: 219 1237 179 1135 681 191 1471 Fragments arranged by size: 1471 1237 1135 681 219 191 179 SfoI GGC'GCC Cuts at: 2052 2052 Size: 5113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5113 SgrAI Cr'CCGG_yG Cuts at: 1700 2102 1700 Size: 402 4711 SmaI CCC'GGG Cuts at: 1872 2440 1872 Size: 568 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3217 4118 4380 820 Size: 197 926 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2904 493 Size: 2411 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5113 SphI G_CATG'C Cuts at: 1132 2471 1132 Size: 1339 3774 SspI AAT'ATT Cuts at: 5 52 3037 5 Size: 47 2985 2081 Fragments arranged by size: 2985 2081 47 StuI AGG'CCT Cuts at: 1517 2300 1517 Size: 783 4330 StyI C'CwwG_G Cuts at: 513 1065 2004 2370 2552 513 Size: 552 939 366 182 3074 Fragments arranged by size: 3074 939 552 366 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3140 3157 3310 3495 3940 325 Size: 1255 1560 17 153 185 445 1498 Fragments arranged by size: 1560 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3359 5071 96 Size: 274 80 33 51 1443 1382 1712 138 Fragments arranged by size: 1712 1443 1382 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5073 5105 5073 Size: 32 5081 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5113 XbaI T'CTAG_A Cuts at: 2449 2449 Size: 5113 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5113 XmaI C'CCGG_G Cuts at: 1870 2438 1870 Size: 568 4545 XmnI GAAnn'nnTTC Cuts at: 3242 3242 Size: 5113 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2921 276 Size: 53 83 186 936 1387 2468 Fragments arranged by size: 2468 1387 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI