(Linear) (Six-base) MAPSORT of: KIEE5136.seq  Check: 9894  from: 1  to: 408



KIEE5136.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AccI GT'mk_AC

Cuts at:      0      31     408 
   Size:         31     377
 
AfeI AGC'GCT

Cuts at:      0      87     408 
   Size:         87     321
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     408 
   Size:        210     198
 
BanI G'GyrC_C

Cuts at:      0      45     133     408 
   Size:         45      88     275

  Fragments arranged by size: 

                 275      88      45
 
BanII G_rGCy'C

Cuts at:      0     262     408 
   Size:        262     146
 
BbeI G_GCGC'C

Cuts at:      0      49     408 
   Size:         49     359
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38     408 
   Size:          4      34     370

  Fragments arranged by size: 

                 370      34       4
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70     408 
   Size:         70     338
 
Bme1580I G_kGCm'C

Cuts at:      0     136     408 
   Size:        136     272
 
BsaHI Gr'CG_yC

Cuts at:      0      46     408 
   Size:         46     362
 
BsiEI CG_ry'CG

Cuts at:      0     130     408 
   Size:        130     278
 
BsiHKAI G_wGCw'C

Cuts at:      0     262     408 
   Size:        262     146
 
Bsp1286I G_dGCh'C

Cuts at:      0     136     262     408 
   Size:        136     126     146

  Fragments arranged by size: 

                 146     136     126
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     254     408 
   Size:        254     154
 
BsrFI r'CCGG_y

Cuts at:      0      97     125     408 
   Size:         97      28     283

  Fragments arranged by size: 

                 283      97      28
 
BstYI r'GATC_y

Cuts at:      0     152     408 
   Size:        152     256
 
BtgI C'CryG_G

Cuts at:      0     137     393     408 
   Size:        137     256      15

  Fragments arranged by size: 

                 256     137      15
 
DraI TTT'AAA

Cuts at:      0     237     408 
   Size:        237     171
 
EaeI y'GGCC_r

Cuts at:      0      62      92     127     408 
   Size:         62      30      35     281

  Fragments arranged by size: 

                 281      62      35      30
 
EagI C'GGCC_G

Cuts at:      0     127     408 
   Size:        127     281
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     408 
   Size:        145     263
 
EcoICRI GAG'CTC

Cuts at:      0     260     408 
   Size:        260     148
 
FspI TGC'GCA

Cuts at:      0     227     408 
   Size:        227     181
 
HaeII r_GCGC'y

Cuts at:      0      49      89     210     408 
   Size:         49      40     121     198

  Fragments arranged by size: 

                 198     121      49      40
 
KasI G'GCGC_C

Cuts at:      0      45     408 
   Size:         45     363
 
MscI TGG'CCA

Cuts at:      0      64     408 
   Size:         64     344
 
MslI CAynn'nnrTG

Cuts at:      0     358     408 
   Size:        358      50
 
MspA1I CmG'CkG

Cuts at:      0     265     395     408 
   Size:        265     130      13

  Fragments arranged by size: 

                 265     130      13
 
NarI GG'CG_CC

Cuts at:      0      46     408 
   Size:         46     362
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     210     408 
   Size:        210     198
 
PstI C_TGCA'G

Cuts at:      0     281     408 
   Size:        281     127
 
SacI G_AGCT'C

Cuts at:      0     262     408 
   Size:        262     146
 
SacII CC_GC'GG

Cuts at:      0     396     408 
   Size:        396      12
 
SfcI C'TryA_G

Cuts at:      0     277     408 
   Size:        277     131
 
SfoI GGC'GCC

Cuts at:      0      47     408 
   Size:         47     361
 
SgrAI Cr'CCGG_yG

Cuts at:      0      97     408 
   Size:         97     311
 
StuI AGG'CCT

Cuts at:      0     295     408 
   Size:        295     113
 
StyI C'CwwG_G

Cuts at:      0     365     408 
   Size:        365      43
 

 Enzymes that do cut:

     AccI     AfeI    AlwNI     BanI    BanII     BbeI     BcgI     BglI
 Bme1580I    BsaHI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI    BstYI
     BtgI     DraI     EaeI     EagI     EciI  EcoICRI     FspI    HaeII
     KasI     MscI     MslI   MspA1I     NarI    PflMI     PstI     SacI
    SacII     SfcI     SfoI    SgrAI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI