################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp718I BbrPI BciVI BfrI BseAI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI DraI EcoRI EcoRV HpaI KpnI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI TfiI Tsp509I XbaI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### Alw44I 4449 GTGCAC G^TGCAC AocI 546 CCTGAGG CC^T[ACGT]AGG ApaLI 4449 GTGCAC G^TGCAC AvrII 2769 CCTAGG C^CTAGG BlnI 2769 CCTAGG C^CTAGG BlpI 49 GCTGAGC GC^T[ACGT]AGC Bpu1102I 49 GCTGAGC GC^T[ACGT]AGC BsaAI 1093 TACGTG [CT]AC^GT[GA] BsaWI 5798 TCCGGT [AT]^CCGG[AT] BsiWI 979 CGTACG C^GTACG Bsp1407I 4157 TGTACA T^GTACA BspLU11I 4271 ACATGT A^CATGT BsrGI 4157 TGTACA T^GTACA Bsu36I 546 CCTGAGG CC^T[ACGT]AGG CelII 49 GCTGAGC GC^T[ACGT]AGC CpoI 6545 CGGACCG CG^G[AT]CCG Eco81I 546 CCTGAGG CC^T[ACGT]AGG EcoT22I 2341 ATGCAT ATGCA^T FseI 6168 GGCCGGCC GGCCGG^CC HindIII 4841 AAGCTT A^AGCTT MseI 5422 TTAA T^TAA NsiI 2341 ATGCAT ATGCA^T RsrII 6545 CGGACCG CG^G[AT]CCG SbfI 342 CCTGCAGG CCTGCA^GG Sse8387I 342 CCTGCAGG CCTGCA^GG SspBI 4157 TGTACA T^GTACA Tru9I 5422 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 898 GTCTAC GT^[AC][GT]AC 3448 GTCTAC AflIII 4271 ACATGT A^C[GA][CT]GT 6041 ACACGT Aor51HI 1250 AGCGCT AGC^GCT 2891 AGCGCT Asp700I 2403 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3468 GAACAGGTTC BamHI 587 GGATCC G^GATCC 5748 GGATCC BbvCI 3779 CCTCAGC CCTCAGC(-5/-2) 6378 CCTCAGC BfaI 2770 CTAG C^TAG 2869 CTAG BsmI 4811 GAATGC GAATGC(1/-1) 5710 GAATGC BstEII 4503 GGTCACC G^GT[ACGT]ACC 5993 GGTCACC BstPI 4503 GGTCACC G^GT[ACGT]ACC 5993 GGTCACC DraIII 2756 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 3372 CACCCTGTG Eco47III 1250 AGCGCT AGC^GCT 2891 AGCGCT EcoO65I 4503 GGTCACC G^GT[ACGT]ACC 5993 GGTCACC MaeI 2770 CTAG C^TAG 2869 CTAG XmnI 2403 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3468 GAACAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 160 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI ApaI AspEI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbeI BbsI BbvI BcgI BclI BcnI BglI BglII BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PaeR7I PflFI PflMI PleI PpuMI PshAI PstI PvuII RcaI RsaI SacI SacII SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfoI SmaI SmlI SphI StuI StyI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI