Restriction map for
pFN21AE2484
(Linear) (Six-base) MAPSORT of: KIEE2484.seq Check: 6334 from: 1 to: 7131
KIEE2484.orf
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1363 3525 5467 7131
Size: 1363 2162 1942 1664
Fragments arranged by size:
2162 1942 1664 1363
AatII G_ACGT'C
Cuts at: 0 5962 7131
Size: 5962 1169
AccI GT'mk_AC
Cuts at: 0 1070 2966 7131
Size: 1070 1896 4165
Fragments arranged by size:
4165 1896 1070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1825 2395 2545 2619 3376 3596 4213 4948
Size: 1825 570 150 74 757 220 617 735
Cuts at: 4948 5179 5188 5860 6748 7131
Size: 231 9 672 888 383
Fragments arranged by size:
1825 888 757 735 672 617 570 383
231 220 150 74 9
AfeI AGC'GCT
Cuts at: 0 337 769 3294 3912 4749 7131
Size: 337 432 2525 618 837 2382
Fragments arranged by size:
2525 2382 837 618 432 337
AflIII A'CryG_T
Cuts at: 0 4589 6155 7131
Size: 4589 1566 976
Fragments arranged by size:
4589 1566 976
AgeI A'CCGG_T
Cuts at: 0 4379 7131
Size: 4379 2752
AhdI GACnn_n'nnGTC
Cuts at: 0 3759 5451 7131
Size: 3759 1692 1680
Fragments arranged by size:
3759 1692 1680
AleI CACnn'nnGTG
Cuts at: 0 5324 7131
Size: 5324 1807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 658 868 2875 3572 7131
Size: 658 210 2007 697 3559
Fragments arranged by size:
3559 2007 697 658 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3908 3940 7131
Size: 3908 32 3191
Fragments arranged by size:
3908 3191 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1442 1654 2757 3229 3287 4191 4425 4914
Size: 1442 212 1103 472 58 904 234 489
Cuts at: 4914 5217 6203 6435 7131
Size: 303 986 232 696
Fragments arranged by size:
1442 1103 986 904 696 489 472 303
234 232 212 58
ApaI G_GGCC'C
Cuts at: 0 1542 1635 1847 1883 2152 3190 3633 5692
Size: 1542 93 212 36 269 1038 443 2059
Cuts at: 5692 6188 6476 6622 7131
Size: 496 288 146 509
Fragments arranged by size:
2059 1542 1038 509 496 443 288 269
212 146 93 36
ApaLI G'TGCA_C
Cuts at: 0 1483 1657 1777 3344 4821 7131
Size: 1483 174 120 1567 1477 2310
Fragments arranged by size:
2310 1567 1483 1477 174 120
ApoI r'AATT_y
Cuts at: 0 735 2712 7131
Size: 735 1977 4419
Fragments arranged by size:
4419 1977 735
AvaI C'yCGr_G
Cuts at: 0 120 1364 1411 1544 1876 2012 2302 3626
Size: 120 1244 47 133 332 136 290 1324
Cuts at: 3626 3745 5150 6491 6946 7131
Size: 119 1405 1341 455 185
Fragments arranged by size:
1405 1341 1324 1244 455 332 290 185
136 133 120 119 47
BamHI G'GATC_C
Cuts at: 0 344 3716 7023 7131
Size: 344 3372 3307 108
Fragments arranged by size:
3372 3307 344 108
BanI G'GyrC_C
Cuts at: 0 25 579 997 1707 1924 3537 3617 4108
Size: 25 554 418 710 217 1613 80 491
Cuts at: 4108 6334 6392 7131
Size: 2226 58 739
Fragments arranged by size:
2226 1613 739 710 554 491 418 217
80 58 25
BanII G_rGCy'C
Cuts at: 0 802 1147 1286 1542 1635 1847 1883 2039
Size: 802 345 139 256 93 212 36 156
Cuts at: 2039 2152 2165 3008 3190 3436 3633 3726 5291
Size: 113 13 843 182 246 197 93 1565
Cuts at: 5291 5692 5724 5824 6188 6212 6476 6622 6710
Size: 401 32 100 364 24 264 146 88
Cuts at: 6710 7005 7131
Size: 295 126
Fragments arranged by size:
1565 843 802 401 364 345 295 264
256 246 212 197 182 156 146 139
126 113 100 93 93 88 36 32
24 13
BbeI G_GCGC'C
Cuts at: 0 29 1001 1711 3621 6396 7131
Size: 29 972 710 1910 2775 735
Fragments arranged by size:
2775 1910 972 735 710 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 654 2334 2584 4689 5632 6455 7131
Size: 654 1680 250 2105 943 823 676
Fragments arranged by size:
2105 1680 943 823 676 654 250
BbvCI CC'TCA_GC
Cuts at: 0 4129 5632 7131
Size: 4129 1503 1499
Fragments arranged by size:
4129 1503 1499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2265 2299 3858 3892 4708 4742 7131
Size: 2265 34 1559 34 816 34 2389
Fragments arranged by size:
2389 2265 1559 816 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 609 1778 4326 7131
Size: 609 1169 2548 2805
Fragments arranged by size:
2805 2548 1169 609
BclI T'GATC_A
Cuts at: 0 1157 4172 7131
Size: 1157 3015 2959
Fragments arranged by size:
3015 2959 1157
BfrBI ATG'CAT
Cuts at: 0 2652 7131
Size: 2652 4479
BglI GCCn_nnn'nGGC
Cuts at: 0 1546 3461 5870 5909 6390 7131
Size: 1546 1915 2409 39 481 741
Fragments arranged by size:
2409 1915 1546 741 481 39
BglII A'GATC_T
Cuts at: 0 1946 4931 6557 7131
Size: 1946 2985 1626 574
Fragments arranged by size:
2985 1946 1626 574
BlpI GC'TnA_GC
Cuts at: 0 969 1393 4740 5606 6661 7131
Size: 969 424 3347 866 1055 470
Fragments arranged by size:
3347 1055 969 866 470 424
Bme1580I G_kGCm'C
Cuts at: 0 584 857 1487 1542 1635 1661 1781 1847
Size: 584 273 630 55 93 26 120 66
Cuts at: 1847 1883 2013 2152 3190 3348 3633 4825 5692
Size: 36 130 139 1038 158 285 1192 867
Cuts at: 5692 6048 6188 6337 6476 6622 6853 7131
Size: 356 140 149 139 146 231 278
Fragments arranged by size:
1192 1038 867 630 584 356 285 278
273 231 158 149 146 140 139 139
130 120 93 66 55 36 26
BmgBI CAC'GTC
Cuts at: 0 2807 7131
Size: 2807 4324
BmrI ACTGGGnnnn_n'
Cuts at: 0 4003 4758 5508 5665 6618 7131
Size: 4003 755 750 157 953 513
Fragments arranged by size:
4003 953 755 750 513 157
BmtI G_CTAG'C
Cuts at: 0 1274 7131
Size: 1274 5857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2184 3450 3745 3771 7019 7131
Size: 2184 1266 295 26 3248 112
Fragments arranged by size:
3248 2184 1266 295 112 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 784 906 1078 1927 1935 2205 2383
Size: 514 270 122 172 849 8 270 178
Cuts at: 2383 3306 3309 3511 4708 5095 5323 5581 5632
Size: 923 3 202 1197 387 228 258 51
Cuts at: 5632 5638 5788 5848 6070 6292 6608 7131
Size: 6 150 60 222 222 316 523
Fragments arranged by size:
1197 923 849 523 514 387 316 270
270 258 228 222 222 202 178 172
150 122 60 51 8 6 3
Bpu10I CC'TnA_GC
Cuts at: 0 562 751 1355 1534 3688 4129 4165 5632
Size: 562 189 604 179 2154 441 36 1467
Cuts at: 5632 7131
Size: 1499
Fragments arranged by size:
2154 1499 1467 604 562 441 189 179
36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 1294 7131
Size: 210 1084 5837
Fragments arranged by size:
5837 1084 210
BsaI GGTCTCn'nnnn_
Cuts at: 0 1192 1365 1417 4101 5291 6622 6671 7131
Size: 1192 173 52 2684 1190 1331 49 460
Fragments arranged by size:
2684 1331 1192 1190 460 173 52 49
BsaAI yAC'GTr
Cuts at: 0 2859 3840 7131
Size: 2859 981 3291
Fragments arranged by size:
3291 2859 981
BsaBI GATnn'nnATC
Cuts at: 0 1036 2254 7131
Size: 1036 1218 4877
Fragments arranged by size:
4877 1218 1036
BsaHI Gr'CG_yC
Cuts at: 0 26 686 998 1615 1708 3618 3668 5959
Size: 26 660 312 617 93 1910 50 2291
Cuts at: 5959 6393 7131
Size: 434 738
Fragments arranged by size:
2291 1910 738 660 617 434 312 93
50 26
BsaWI w'CCGG_w
Cuts at: 0 1794 2705 3809 4379 6992 7096 7131
Size: 1794 911 1104 570 2613 104 35
Fragments arranged by size:
2613 1794 1104 911 570 104 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 204 234 904 934 1773 1803 4003 4033
Size: 204 30 670 30 839 30 2200 30
Cuts at: 4033 5935 5965 6817 6847 7131
Size: 1902 30 852 30 284
Fragments arranged by size:
2200 1902 852 839 670 284 204 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 25 46 1120 1315 2320 3442 3457
Size: 22 3 21 1074 195 1005 1122 15
Cuts at: 3457 3770 4663 4681 4795 5578 5602 6718 6810
Size: 313 893 18 114 783 24 1116 92
Cuts at: 6810 7011 7131
Size: 201 120
Fragments arranged by size:
1122 1116 1074 1005 893 783 313 201
195 120 114 92 24 22 21 18
15 3
BseYI C'CCAG_C
Cuts at: 0 332 583 630 815 1064 1401 1427 1432
Size: 332 251 47 185 249 337 26 5
Cuts at: 1432 2099 2164 3022 3183 3259 3349 5639 5730
Size: 667 65 858 161 76 90 2290 91
Cuts at: 5730 5899 6203 6251 6973 7113 7131
Size: 169 304 48 722 140 18
Fragments arranged by size:
2290 858 722 667 337 332 304 251
249 185 169 161 140 91 90 76
65 48 47 26 18 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4312 5710 7131
Size: 4312 1398 1421
Fragments arranged by size:
4312 1421 1398
BsiEI CG_ry'CG
Cuts at: 0 94 559 2046 3131 3342 7131
Size: 94 465 1487 1085 211 3789
Fragments arranged by size:
3789 1487 1085 465 211 94
BsiHKAI G_wGCw'C
Cuts at: 0 983 1487 1661 1781 2039 2573 2864 3083
Size: 983 504 174 120 258 534 291 219
Cuts at: 3083 3160 3348 3436 3587 4064 4825 4895 5291
Size: 77 188 88 151 477 761 70 396
Cuts at: 5291 5774 6175 7131
Size: 483 401 956
Fragments arranged by size:
983 956 761 534 504 483 477 401
396 291 258 219 188 174 151 120
88 77 70
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1792 4201 7131
Size: 1792 2409 2930
Fragments arranged by size:
2930 2409 1792
Bsp1286I G_dGCh'C
Cuts at: 0 584 802 857 983 1147 1286 1487 1542
Size: 584 218 55 126 164 139 201 55
Cuts at: 1542 1635 1661 1781 1847 1883 2013 2039 2152
Size: 93 26 120 66 36 130 26 113
Cuts at: 2152 2165 2573 2864 3008 3083 3160 3190 3348
Size: 13 408 291 144 75 77 30 158
Cuts at: 3348 3436 3587 3633 3726 4064 4825 4895 5291
Size: 88 151 46 93 338 761 70 396
Cuts at: 5291 5692 5724 5774 5824 6048 6175 6188 6212
Size: 401 32 50 50 224 127 13 24
Cuts at: 6212 6337 6476 6622 6710 6853 7005 7131
Size: 125 139 146 88 143 152 126
Fragments arranged by size:
761 584 408 401 396 338 291 224
218 201 164 158 152 151 146 144
143 139 139 130 127 126 126 125
120 113 93 93 88 88 77 75
70 66 55 55 50 50 46 36
32 30 26 26 24 13 13
BspEI T'CCGG_A
Cuts at: 0 6992 7131
Size: 6992 139
BspHI T'CATG_A
Cuts at: 0 1154 2246 2573 2840 4517 5129 7131
Size: 1154 1092 327 267 1677 612 2002
Fragments arranged by size:
2002 1677 1154 1092 612 327 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 518 1363 3525 3527 4031 5467 6461 7131
Size: 518 845 2162 2 504 1436 994 670
Fragments arranged by size:
2162 1436 994 845 670 518 504 2
BsrBI CCG'CTC
Cuts at: 0 22 561 2682 3381 3447 3495 4164 7131
Size: 22 539 2121 699 66 48 669 2967
Fragments arranged by size:
2967 2121 699 669 539 66 48 22
BsrDI GCAATG_nn'
Cuts at: 0 1530 2831 3603 5144 7131
Size: 1530 1301 772 1541 1987
Fragments arranged by size:
1987 1541 1530 1301 772
BsrFI r'CCGG_y
Cuts at: 0 89 458 1589 2129 2544 4379 6767 6899
Size: 89 369 1131 540 415 1835 2388 132
Cuts at: 6899 7131
Size: 232
Fragments arranged by size:
2388 1835 1131 540 415 369 232 132
89
BsrGI T'GTAC_A
Cuts at: 0 1322 7131
Size: 1322 5809
BssSI C'ACGA_G
Cuts at: 0 2293 3241 7131
Size: 2293 948 3890
Fragments arranged by size:
3890 2293 948
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1239 1442 1902 5217 6138 7131
Size: 1239 203 460 3315 921 993
Fragments arranged by size:
3315 1239 993 921 460 203
BstEII G'GTnAC_C
Cuts at: 0 4875 7131
Size: 4875 2256
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1266 2889 3882 4119 4887 6883 7131
Size: 1266 1623 993 237 768 1996 248
Fragments arranged by size:
1996 1623 1266 993 768 248 237
BstYI r'GATC_y
Cuts at: 0 344 1559 1946 2753 3716 4115 4931 5924
Size: 344 1215 387 807 963 399 816 993
Cuts at: 5924 6557 7023 7131
Size: 633 466 108
Fragments arranged by size:
1215 993 963 816 807 633 466 399
387 344 108
BtgI C'CryG_G
Cuts at: 0 208 386 2582 3881 4091 4880 4886 5777
Size: 208 178 2196 1299 210 789 6 891
Cuts at: 5777 6074 6718 7131
Size: 297 644 413
Fragments arranged by size:
2196 1299 891 789 644 413 297 210
208 178 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 303 465 3457 3535 3861 7131
Size: 303 162 2992 78 326 3270
Fragments arranged by size:
3270 2992 326 303 162 78
BtsI GCAGTG_nn'
Cuts at: 0 3471 3529 3554 6173 7131
Size: 3471 58 25 2619 958
Fragments arranged by size:
3471 2619 958 58 25
DraIII CAC_nnn'GTG
Cuts at: 0 1482 7131
Size: 1482 5649
DrdI GACnn_nn'nnGTC
Cuts at: 0 3841 4215 7131
Size: 3841 374 2916
Fragments arranged by size:
3841 2916 374
EaeI y'GGCC_r
Cuts at: 0 91 304 2235 2594 3128 3339 3564 4416
Size: 91 213 1931 359 534 211 225 852
Cuts at: 4416 4883 5039 6696 7131
Size: 467 156 1657 435
Fragments arranged by size:
1931 1657 852 534 467 435 359 225
213 211 156 91
EagI C'GGCC_G
Cuts at: 0 91 3128 3339 7131
Size: 91 3037 211 3792
Fragments arranged by size:
3792 3037 211 91
EarI CTCTTCn'nnn_
Cuts at: 0 2626 2662 2869 3091 3419 3434 4303 4640
Size: 2626 36 207 222 328 15 869 337
Cuts at: 4640 4849 5251 5389 7131
Size: 209 402 138 1742
Fragments arranged by size:
2626 1742 869 402 337 328 222 209
207 138 36 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 2497 2920 3784 7131
Size: 157 2340 423 864 3347
Fragments arranged by size:
3347 2340 864 423 157
EcoICRI GAG'CTC
Cuts at: 0 2037 3434 5289 7131
Size: 2037 1397 1855 1842
Fragments arranged by size:
2037 1855 1842 1397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 784 906 1078 1825 1927 1935 2205
Size: 514 270 122 172 747 102 8 270
Cuts at: 2205 2383 2395 2545 2619 3306 3309 3376 3511
Size: 178 12 150 74 687 3 67 135
Cuts at: 3511 3596 4213 4708 4948 5095 5179 5188 5323
Size: 85 617 495 240 147 84 9 135
Cuts at: 5323 5581 5632 5638 5788 5848 5860 6070 6292
Size: 258 51 6 150 60 12 210 222
Cuts at: 6292 6608 6748 7131
Size: 316 140 383
Fragments arranged by size:
747 687 617 514 495 383 316 270
270 258 240 222 210 178 172 150
150 147 140 135 135 122 102 85
84 74 67 60 51 12 12 9
8 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1333 2184 2761 3175 4417 5221 6590 7131
Size: 1333 851 577 414 1242 804 1369 541
Fragments arranged by size:
1369 1333 1242 851 804 577 541 414
EcoO109I rG'GnC_Cy
Cuts at: 0 117 1538 1539 1583 1631 1632 1721 1844
Size: 117 1421 1 44 48 1 89 123
Cuts at: 1844 1880 2118 3187 3485 3630 4422 5214 5331
Size: 36 238 1069 298 145 792 792 117
Cuts at: 5331 5688 5689 6184 6185 6473 6618 6619 6723
Size: 357 1 495 1 288 145 1 104
Cuts at: 6723 6889 6958 7131
Size: 166 69 173
Fragments arranged by size:
1421 1069 792 792 495 357 298 288
238 173 166 145 145 123 117 117
104 89 69 48 44 36 1 1
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3072 3528 4419 7044 7131
Size: 3072 456 891 2625 87
Fragments arranged by size:
3072 2625 891 456 87
FseI GG_CCGG'CC
Cuts at: 0 93 7131
Size: 93 7038
FspI TGC'GCA
Cuts at: 0 592 5143 7131
Size: 592 4551 1988
Fragments arranged by size:
4551 1988 592
FspAI rTGC'GCAy
Cuts at: 0 592 7131
Size: 592 6539
HaeII r_GCGC'y
Cuts at: 0 29 166 339 771 1001 1711 2551 3184
Size: 29 137 173 432 230 710 840 633
Cuts at: 3184 3266 3296 3321 3621 3891 3914 4677 4751
Size: 82 30 25 300 270 23 763 74
Cuts at: 4751 5194 6323 6396 6768 7131
Size: 443 1129 73 372 363
Fragments arranged by size:
1129 840 763 710 633 443 432 372
363 300 270 230 173 137 82 74
73 30 29 25 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 236 1080 1112 3001 3033 3235 3267
Size: 204 32 844 32 1889 32 202 32
Cuts at: 3267 3370 3402 3745 3777 4689 4721 5437 5469
Size: 103 32 343 32 912 32 716 32
Cuts at: 5469 5788 5820 5931 5935 5956 5963 5967 5988
Size: 319 32 111 4 21 7 4 21
Cuts at: 5988 6697 6729 6791 6817 6823 6849 6942 6974
Size: 709 32 62 26 6 26 93 32
Cuts at: 6974 7131
Size: 157
Fragments arranged by size:
1889 912 844 716 709 343 319 204
202 157 111 103 93 62 32 32
32 32 32 32 32 32 32 32
32 26 26 21 21 7 6 4
4
HincII GTy'rAC
Cuts at: 0 942 1071 2265 2967 5091 5529 7131
Size: 942 129 1194 702 2124 438 1602
Fragments arranged by size:
2124 1602 1194 942 702 438 129
KasI G'GCGC_C
Cuts at: 0 25 997 1707 3617 6392 7131
Size: 25 972 710 1910 2775 739
Fragments arranged by size:
2775 1910 972 739 710 25
MfeI C'AATT_G
Cuts at: 0 5406 7131
Size: 5406 1725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 4015 4808 5939 6629 7131
Size: 1277 2738 793 1131 690 502
Fragments arranged by size:
2738 1277 1131 793 690 502
MscI TGG'CCA
Cuts at: 0 2596 4418 4885 5041 7131
Size: 2596 1822 467 156 2090
Fragments arranged by size:
2596 2090 1822 467 156
MslI CAynn'nnrTG
Cuts at: 0 259 1138 2452 2887 3139 3880 4084 4594
Size: 259 879 1314 435 252 741 204 510
Cuts at: 4594 4885 5134 5206 5324 7131
Size: 291 249 72 118 1807
Fragments arranged by size:
1807 1314 879 741 510 435 291 259
252 249 204 118 72
MspA1I CmG'CkG
Cuts at: 0 210 532 693 973 1209 1221 1292 1442
Size: 210 322 161 280 236 12 71 150
Cuts at: 1442 1700 1757 1997 2556 2562 2958 3215 3305
Size: 258 57 240 559 6 396 257 90
Cuts at: 3305 4106 5031 5734 5790 6818 6830 7131
Size: 801 925 703 56 1028 12 301
Fragments arranged by size:
1028 925 801 703 559 396 322 301
280 258 257 240 236 210 161 150
90 71 57 56 12 12 6
NaeI GCC'GGC
Cuts at: 0 91 1591 2131 2546 6769 6901 7131
Size: 91 1500 540 415 4223 132 230
Fragments arranged by size:
4223 1500 540 415 230 132 91
NarI GG'CG_CC
Cuts at: 0 26 998 1708 3618 6393 7131
Size: 26 972 710 1910 2775 738
Fragments arranged by size:
2775 1910 972 738 710 26
NcoI C'CATG_G
Cuts at: 0 2582 3881 4091 4880 4886 7131
Size: 2582 1299 210 789 6 2245
Fragments arranged by size:
2582 2245 1299 789 210 6
NgoMIV G'CCGG_C
Cuts at: 0 89 1589 2129 2544 6767 6899 7131
Size: 89 1500 540 415 4223 132 232
Fragments arranged by size:
4223 1500 540 415 232 132 89
NheI G'CTAG_C
Cuts at: 0 1270 7131
Size: 1270 5861
NsiI A_TGCA'T
Cuts at: 0 2654 7131
Size: 2654 4477
NspI r_CATG'y
Cuts at: 0 264 300 591 837 2610 2652 4128 4593
Size: 264 36 291 246 1773 42 1476 465
Cuts at: 4593 5187 6027 6159 7131
Size: 594 840 132 972
Fragments arranged by size:
1773 1476 972 840 594 465 291 264
246 132 42 36
PasI CC'CwG_GG
Cuts at: 0 291 2973 3072 5866 6189 7131
Size: 291 2682 99 2794 323 942
Fragments arranged by size:
2794 2682 942 323 291 99
PciI A'CATG_T
Cuts at: 0 4589 6155 7131
Size: 4589 1566 976
Fragments arranged by size:
4589 1566 976
PflMI CCAn_nnn'nTGG
Cuts at: 0 1479 3658 4836 4914 7131
Size: 1479 2179 1178 78 2217
Fragments arranged by size:
2217 2179 1479 1178 78
PfoI T'CCnGG_A
Cuts at: 0 1562 2114 5012 5435 7131
Size: 1562 552 2898 423 1696
Fragments arranged by size:
2898 1696 1562 552 423
PmlI CAC'GTG
Cuts at: 0 3840 7131
Size: 3840 3291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6120 6152 7131
Size: 6120 32 979
Fragments arranged by size:
6120 979 32
PpuMI rG'GwC_Cy
Cuts at: 0 117 1583 1721 2118 3485 6723 6958 7131
Size: 117 1466 138 397 1367 3238 235 173
Fragments arranged by size:
3238 1466 1367 397 235 173 138 117
PspOMI G'GGCC_C
Cuts at: 0 1538 1631 1843 1879 2148 3186 3629 5688
Size: 1538 93 212 36 269 1038 443 2059
Cuts at: 5688 6184 6472 6618 7131
Size: 496 288 146 513
Fragments arranged by size:
2059 1538 1038 513 496 443 288 269
212 146 93 36
PspXI vC'TCGA_Gb
Cuts at: 0 3745 7131
Size: 3745 3386
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 940 972 7131
Size: 940 32 6159
Fragments arranged by size:
6159 940 32
PstI C_TGCA'G
Cuts at: 0 353 515 847 2555 3536 6281 7131
Size: 353 162 332 1708 981 2745 850
Fragments arranged by size:
2745 1708 981 850 353 332 162
PvuII CAG'CTG
Cuts at: 0 532 693 1221 1292 1442 1997 2562 2958
Size: 532 161 528 71 150 555 565 396
Cuts at: 2958 3305 5031 5790 6818 7131
Size: 347 1726 759 1028 313
Fragments arranged by size:
1726 1028 759 565 555 532 528 396
347 313 161 150 71
SacI G_AGCT'C
Cuts at: 0 2039 3436 5291 7131
Size: 2039 1397 1855 1840
Fragments arranged by size:
2039 1855 1840 1397
SacII CC_GC'GG
Cuts at: 0 211 7131
Size: 211 6920
SalI G'TCGA_C
Cuts at: 0 1069 2965 7131
Size: 1069 1896 4166
Fragments arranged by size:
4166 1896 1069
SanDI GG'GwC_CC
Cuts at: 0 1583 2118 3485 7131
Size: 1583 535 1367 3646
Fragments arranged by size:
3646 1583 1367 535
SapI GCTCTTCn'nnn_
Cuts at: 0 2626 2869 3091 4303 7131
Size: 2626 243 222 1212 2828
Fragments arranged by size:
2828 2626 1212 243 222
SbfI CC_TGCA'GG
Cuts at: 0 353 7131
Size: 353 6778
ScaI AGT'ACT
Cuts at: 0 499 955 2230 7131
Size: 499 456 1275 4901
Fragments arranged by size:
4901 1275 499 456
SexAI A'CCwGG_T
Cuts at: 0 1316 1478 7131
Size: 1316 162 5653
Fragments arranged by size:
5653 1316 162
SfcI C'TryA_G
Cuts at: 0 349 511 843 1884 2551 3532 6277 6738
Size: 349 162 332 1041 667 981 2745 461
Cuts at: 6738 7131
Size: 393
Fragments arranged by size:
2745 1041 981 667 461 393 349 332
162
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1546 5870 7131
Size: 1546 4324 1261
Fragments arranged by size:
4324 1546 1261
SfoI GGC'GCC
Cuts at: 0 27 999 1709 3619 6394 7131
Size: 27 972 710 1910 2775 737
Fragments arranged by size:
2775 1910 972 737 710 27
SmaI CCC'GGG
Cuts at: 0 122 1546 1878 2014 3628 6493 7131
Size: 122 1424 332 136 1614 2865 638
Fragments arranged by size:
2865 1614 1424 638 332 136 122
SmlI C'TyrA_G
Cuts at: 0 189 1309 3745 7131
Size: 189 1120 2436 3386
Fragments arranged by size:
3386 2436 1120 189
SpeI A'CTAG_T
Cuts at: 0 1818 7131
Size: 1818 5313
SphI G_CATG'C
Cuts at: 0 591 837 2610 2652 4128 5187 7131
Size: 591 246 1773 42 1476 1059 1944
Fragments arranged by size:
1944 1773 1476 1059 591 246 42
SrfI GCCC'GGGC
Cuts at: 0 2014 3628 6493 7131
Size: 2014 1614 2865 638
Fragments arranged by size:
2865 2014 1614 638
StuI AGG'CCT
Cuts at: 0 355 910 7131
Size: 355 555 6221
Fragments arranged by size:
6221 555 355
StyI C'CwwG_G
Cuts at: 0 413 2582 3062 3881 4091 4413 4451 4880
Size: 413 2169 480 819 210 322 38 429
Cuts at: 4880 4886 5582 6419 6867 6954 7019 7028 7131
Size: 6 696 837 448 87 65 9 103
Fragments arranged by size:
2169 837 819 696 480 448 429 413
322 210 103 87 65 38 9 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3036 3153 3363 3835 4452 4588 5744 7131
Size: 3036 117 210 472 617 136 1156 1387
Fragments arranged by size:
3036 1387 1156 617 472 210 136 117
TatI w'GTAC_w
Cuts at: 0 497 953 1079 1322 1918 2228 4397 4544
Size: 497 456 126 243 596 310 2169 147
Cuts at: 4544 7131
Size: 2587
Fragments arranged by size:
2587 2169 596 497 456 310 243 147
126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 531 563 1773 1805 3469 3501 7131
Size: 531 32 1210 32 1664 32 3630
Fragments arranged by size:
3630 1664 1210 531 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1522 2172 2589 3168 3595 4833 6605 7026
Size: 1522 650 417 579 427 1238 1772 421
Cuts at: 7026 7131
Size: 105
Fragments arranged by size:
1772 1522 1238 650 579 427 421 417
105
XhoI C'TCGA_G
Cuts at: 0 3745 7131
Size: 3745 3386
XmaI C'CCGG_G
Cuts at: 0 120 1544 1876 2012 3626 6491 7131
Size: 120 1424 332 136 1614 2865 640
Fragments arranged by size:
2865 1614 1424 640 332 136 120
XmnI GAAnn'nnTTC
Cuts at: 0 4312 7131
Size: 4312 2819
ZraI GAC'GTC
Cuts at: 0 5960 7131
Size: 5960 1171
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI FspAI HaeII
Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI
PsrI PstI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AscI AseI AsiSI AvrII BaeI
BsiWI BsmI BssHII BstBI BstZ17I Bsu36I ClaI CspCI
DraI EcoRI EcoRV HindIII HpaI KpnI MluI NdeI
NotI NruI PacI PmeI PshAI PsiI PvuI RsrII
SgfI SgrAI SnaBI SspI SwaI Tth111I XbaI