(Circular) (Six-base) MAPSORT of: pFN21AB5943.seq Check: 9506 from: 1 to: 6229 pFN21AB5943 6229 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2730 2804 4292 5676 5944 1632 Size: 1098 74 1488 1384 268 1917 Fragments arranged by size: 1917 1488 1384 1098 268 74 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 2350 5060 2350 Size: 2710 3519 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2079 1184 Size: 895 5334 AlwNI CAG_nnn'CTG Cuts at: 2591 3972 5544 2591 Size: 1381 1572 3276 Fragments arranged by size: 3276 1572 1381 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 2096 2643 2855 3477 3538 3764 3916 6025 Size: 603 547 212 622 61 226 152 2109 Cuts at: 6025 1493 Size: 1697 Fragments arranged by size: 2109 1697 622 603 547 226 212 152 61 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2242 3554 1870 Size: 73 299 1312 4545 Fragments arranged by size: 4545 1312 299 73 BamHI G'GATC_C Cuts at: 2008 3559 3926 2008 Size: 1551 367 4311 Fragments arranged by size: 4311 1551 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2063 3550 5005 618 Size: 325 217 627 276 1487 1455 1842 Fragments arranged by size: 1842 1487 1455 627 325 276 217 BanII G_rGCy'C Cuts at: 1391 2036 2205 2307 3548 1391 Size: 645 169 102 1241 4072 Fragments arranged by size: 4072 1241 645 169 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2107 2432 2565 2792 2901 928 Size: 971 208 325 133 227 109 4256 Fragments arranged by size: 4256 971 325 227 208 133 109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3311 3345 4420 4454 1903 Size: 34 1374 34 1075 34 3678 Fragments arranged by size: 3678 1374 1075 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BfrBI ATG'CAT Cuts at: 2334 2334 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 4840 136 Size: 107 122 71 631 605 400 2768 1525 Fragments arranged by size: 2768 1525 631 605 400 122 107 71 BglII A'GATC_T Cuts at: 2451 6224 2451 Size: 3773 2456 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 3317 4290 5446 1790 Size: 1527 973 1156 2573 Fragments arranged by size: 2573 1527 1156 973 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2452 3960 4918 Size: 416 53 83 399 24 1035 1508 958 Cuts at: 4918 442 Size: 1753 Fragments arranged by size: 1753 1508 1035 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2174 2257 2174 Size: 83 6146 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 4889 1239 Size: 1040 2610 2579 Fragments arranged by size: 2610 2579 1040 Bpu10I CC'TnA_GC Cuts at: 3164 3164 Size: 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2050 2070 4354 5219 5517 2050 Size: 20 2284 865 298 2762 Fragments arranged by size: 2762 2284 865 298 20 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2041 3366 4892 882 Size: 617 542 1325 1526 2219 Fragments arranged by size: 2219 1526 1325 617 542 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 2743 6223 2743 Size: 3480 2749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4036 4418 5053 275 Size: 53 83 186 936 2503 382 635 1451 Fragments arranged by size: 2503 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2350 2974 3006 4662 5060 5334 5481 5892 2350 Size: 624 32 1656 398 274 147 411 2687 Fragments arranged by size: 2687 1656 624 411 398 274 147 32 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2289 2319 1178 Size: 30 165 30 886 30 5088 Fragments arranged by size: 5088 886 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2068 2073 2297 2696 1186 Size: 882 5 224 399 4719 Fragments arranged by size: 4719 882 399 224 5 BseYI C'CCAG_C Cuts at: 5432 5928 5432 Size: 496 5733 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5656 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 4440 5468 1540 Size: 275 186 1678 761 1028 2301 Fragments arranged by size: 2301 1678 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2205 2307 4290 4375 5446 2205 Size: 102 1983 85 1071 2988 Fragments arranged by size: 2988 1983 1071 102 85 BsmI GAATG_Cn' Cuts at: 2336 2871 3740 3833 2336 Size: 535 869 93 4732 Fragments arranged by size: 4732 869 535 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2205 2307 3317 3548 4290 4375 Size: 399 246 169 102 1010 231 742 85 Cuts at: 4375 5446 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1010 742 399 246 231 169 102 85 BspEI T'CCGG_A Cuts at: 2974 3006 5892 2974 Size: 32 2886 3311 Fragments arranged by size: 3311 2886 32 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3584 844 Size: 2740 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3400 4116 1394 Size: 429 1577 716 3507 Fragments arranged by size: 3507 1577 716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2350 4873 5060 1700 Size: 110 540 2523 187 2869 Fragments arranged by size: 2869 2523 540 187 110 BsrGI T'GTAC_A Cuts at: 96 2443 96 Size: 2347 3882 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 2016 4289 5301 2016 Size: 2273 1012 2944 Fragments arranged by size: 2944 2273 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 3298 3298 Size: 6229 BstYI r'GATC_y Cuts at: 1972 2008 2451 3559 3926 4311 4328 5780 5866 Size: 36 443 1108 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1108 443 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3512 513 Size: 552 939 1508 3230 Fragments arranged by size: 3230 1508 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 3745 4539 4559 3745 Size: 794 20 5415 Fragments arranged by size: 5415 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 2487 3533 2487 Size: 1046 5183 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3676 4565 6194 Size: 54 1425 50 275 18 1846 889 1629 Cuts at: 6194 8 Size: 43 Fragments arranged by size: 1846 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2383 3069 4159 1353 Size: 686 344 686 1090 3423 Fragments arranged by size: 3423 1090 686 686 344 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2203 2305 2203 Size: 102 6127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2730 2804 4292 4889 5676 5944 Size: 393 647 451 74 1488 597 787 268 Cuts at: 5944 1239 Size: 1524 Fragments arranged by size: 1524 1488 787 647 597 451 393 268 74 EcoO109I rG'GnC_Cy Cuts at: 2417 2417 Size: 6229 EcoRI G'AATT_C Cuts at: 2643 3538 3916 2643 Size: 895 378 4956 Fragments arranged by size: 4956 895 378 EcoRV GAT'ATC Cuts at: 2741 2741 Size: 6229 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2499 3098 2499 Size: 599 5630 FspI TGC'GCA Cuts at: 3486 4735 3486 Size: 1249 4980 HaeII r_GCGC'y Cuts at: 1678 3385 5376 1678 Size: 1707 1991 2531 Fragments arranged by size: 2531 1991 1707 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373 Size: 32 135 32 3298 32 42 32 2626 Fragments arranged by size: 3298 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3573 3819 669 Size: 988 281 1212 423 246 3079 Fragments arranged by size: 3079 1212 988 423 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 2436 3828 2436 Size: 1392 4837 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 5343 5527 1305 Size: 1515 2523 184 2007 Fragments arranged by size: 2523 2007 1515 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4407 Fragments arranged by size: 4407 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 3296 4187 4546 4705 518 Size: 648 1458 672 891 359 159 2042 Fragments arranged by size: 2042 1458 891 672 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2907 3264 3645 4322 5470 5715 1606 Size: 1301 357 381 677 1148 245 2120 Fragments arranged by size: 2120 1301 1148 677 381 357 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4738 Fragments arranged by size: 4738 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NsiI A_TGCA'T Cuts at: 2336 2336 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6229 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2786 2818 4298 4330 5811 5843 2786 Size: 32 1480 32 1481 32 3172 Fragments arranged by size: 3172 1481 1480 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PstI C_TGCA'G Cuts at: 830 3581 830 Size: 2751 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2907 3264 2907 Size: 357 5872 SacI G_AGCT'C Cuts at: 2205 2307 2205 Size: 102 6127 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SapI GCTCTTCn'nnn_ Cuts at: 3069 3069 Size: 6229 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SfcI C'TryA_G Cuts at: 826 1045 3577 4712 5393 5584 826 Size: 219 2532 1135 681 191 1471 Fragments arranged by size: 2532 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2029 2085 4333 5234 5496 820 Size: 197 926 86 56 2248 901 262 1553 Fragments arranged by size: 2248 1553 926 901 262 197 86 56 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6229 StyI C'CwwG_G Cuts at: 513 1065 2004 2661 3668 513 Size: 552 939 657 1007 3074 Fragments arranged by size: 3074 1007 939 657 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2234 4256 4273 4426 4611 5056 325 Size: 1255 654 2022 17 153 185 445 1498 Fragments arranged by size: 2022 1498 1255 654 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2281 2443 4475 Size: 274 80 33 51 1443 304 162 2032 Cuts at: 4475 6187 96 Size: 1712 138 Fragments arranged by size: 2032 1712 1443 304 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 2900 1316 Size: 1584 4645 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 2183 2643 2801 4358 2183 Size: 460 158 1557 4054 Fragments arranged by size: 4054 1557 460 158 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI