################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI BbrPI BclI BfrI BglI BglII BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FauI FbaI FseI HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MroI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcyI 1374 GGCGCC G[GA]^CG[CT]C AflIII 301 ACATGT A^C[GA][CT]GT AocI 334 CCTGAGG CC^T[ACGT]AGG AviII 1348 TGCGCA TGC^GCA AvrII 807 CCTAGG C^CTAGG BamHI 768 GGATCC G^GATCC BanI 1374 GGCGCC G^G[CT][GA]CC BanII 330 GAGCCC G[GA]GC[CT]^C BbeI 1374 GGCGCC GGCGC^C BbsI 680 GAAGAC GAAGAC(2/6) BbvCI 1369 GCTGAGG CCTCAGC(-5/-2) BcgI 1463 GCAGGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1246 GTATCC GTATCC(6/5) BcnI 948 CCGGG CC^[GC]GG BlnI 807 CCTAGG C^CTAGG BlpI 246 GCTAAGC GC^T[ACGT]AGC Bpu1102I 246 GCTAAGC GC^T[ACGT]AGC BpuAI 680 GAAGAC GAAGAC(2/6) BsaHI 1374 GGCGCC G[GA]^CG[CT]C BspLU11I 301 ACATGT A^CATGT BspMI 523 ACCTGC ACCTGC(4/8) BsrFI 638 GCCGGC [GA]^CCGG[CT] BstAPI 852 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 708 GGTTACC G^GT[ACGT]ACC BstPI 708 GGTTACC G^GT[ACGT]ACC Bsu36I 334 CCTGAGG CC^T[ACGT]AGG CelII 246 GCTAAGC GC^T[ACGT]AGC Cfr10I 638 GCCGGC [GA]^CCGG[CT] DraII 476 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 334 CCTGAGG CC^T[ACGT]AGG EcoNI 1566 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 476 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 708 GGTTACC G^GT[ACGT]ACC FspI 1348 TGCGCA TGC^GCA HaeII 1374 GGCGCC [GA]GCGC^[CT] HgaI 1258 GACGC GACGC(5/10) Hin1I 1374 GGCGCC G[GA]^CG[CT]C HincII 139 GTTGAC GT[CT]^[GA]AC HindII 139 GTTGAC GT[CT]^[GA]AC KasI 1374 GGCGCC G^GCGCC NaeI 638 GCCGGC GCC^GGC NarI 1374 GGCGCC GG^CGCC NciI 948 CCGGG CC^[GC]GG NgoMIV 638 GCCGGC G^CCGGC PpuMI 476 AGGACCT [GA]G^G[AT]CC[CT] PvuII 1501 CAGCTG CAG^CTG ScaI 933 AGTACT AGT^ACT SfoI 1374 GGCGCC GGC^GCC SpeI 1476 ACTAGT A^CTAGT TaqI 974 TCGA T^CGA TthHB8I 974 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 519 GTCTAC GT^[AC][GT]AC 1255 GTAGAC AciI 834 CCGC CCGC(-3/-1) 1668 CCGC AfaI 934 GTAC GT^AC 1570 GTAC AlwNI 1498 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1561 CAGCTCCTG BalI 531 TGGCCA TGG^CCA 1526 TGGCCA BsmFI 1467 GGGAC GGGAC(10/14) 199 GTCCC HapII 639 CCGG C^CGG 948 CCGG HpaII 639 CCGG C^CGG 948 CCGG MluNI 531 TGGCCA TGG^CCA 1526 TGGCCA MscI 531 TGGCCA TGG^CCA 1526 TGGCCA MseI 356 TTAA T^TAA 745 TTAA MspA1I 834 CCGCTG C[AC]G^C[GT]G 1501 CAGCTG MspI 639 CCGG C^CGG 948 CCGG NcoI 824 CCATGG C^CATGG 1241 CCATGG NspI 301 ACATGT [GA]CATG^[CT] 1345 ACATGC PstI 817 CTGCAG CTGCA^G 1282 CTGCAG RsaI 934 GTAC GT^AC 1570 GTAC SfaNI 635 GATGC GCATC(5/9) 391 GCATC SfcI 817 CTGCAG C^T[GA][CT]AG 1282 CTGCAG Tru9I 356 TTAA T^TAA 745 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AcsI AluI AlwI ApoI Asp700I AspHI AvaII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI StyI TfiI TseI Tsp45I Tsp509I TspRI XhoII XmnI